수학과 교육과정 개선에 관한 연구

신현성 한국수학교육학회 1989 수학교육 Vol.28 No.2

血液培養에 關한 調査硏究 : Salmonella을 中心으로

申鉉成,李敏雄 동국대학교 대학원 1980 연구논집 Vol.10 No.

人蔘圃의 土壤細菌 分布에 關한 硏究

李敏雄,申鉉成 忠南大學校 環境問題硏究所 1995 環境硏究 Vol.3 No.2

The Correlations between environmental influences on microorganisms in soil and its effects on dis- ease development in ginseng field were studied to obtain some useful data for increasing ginseng produc- tion and effective preventive measures against the root rot caused by soil-borne plant pathogenic micor- organisms in soil. The diseased replanted ginseng fields were selected as the diseased field and the healthy plot in first planted field selected as control in three major Korean ginseng producing areas such as Kum- san, Goesan and Poonggi. The physicochemical characteristics of the soil were analyzed, and micororganism suspectable for root rot ginseng, and other general bacteria were investigated for their population density in various soil conditions. Correlations between soil microbial populations, environmental factors were investigat- ed. The results were as follows: 1. The numbers of fluorescent Pseudomonas spp. were abundant in fall season in healthy first planted field and abundant in hot summer season in the diseased replanted field. In comparison of the rela- tive ratio of the fluorescent pseudomonas spp. in replanted field to healthy field in three areas, the number of fluorescent Pseudomonas spp. were 1.1 to 3.8 times higher in replanted field, and also in ratio of fluorescent Pseudomonas spp. to total bacteria, the numbers of fluorescent Pseudomonas spp. populated in replanted field were 1.3 to 4.9 times higher. Those bacterial cells were highly populated in 10-15 ㎝ depthof rhizosphere of ginseng. 2. The numbers of Erwinia spp. were significantly high in spring and fall in Kumsan and Poonggi. In comparison of the relative ratio of Erwinia spp. in replanted field to healthy field in two areas, the numbers of Erwinia spp. were 2.6 to 4.8 times higher in replanted field, and the numbers of Er- winia spp. relative to total bacteria were 3 to 5 times higher in replanted field than first planted field. In comparison of the relative ratioof Erwinia spp. to fluorescent Pseudomonas spp. in first planted fields of two areas, the range of ratio of two different genus of bacteria was 2.5 : &, which was highly discrepant, on the other hand the ratio in replanted field was 6 : 9, which was very close between the two different genus of bacteria. 3. The numbers of total bacteria in each sampled soil were all high in June through September. There was no significant difference in the vertical distributions of total bacteria according to the depth. There was no significant difference between healthy first planted field and diseased replanted field, in the numbers of total bacteria. 4. The contents of organic matters and phosphate in healthy first planted field were somewhat high, and Phophate / Organic matter ratio and Mg content were high in diseased replanted field. All of the soil samples showed a weak acidic pH of 4.5 to 5.7. Soil moisture content was increased during winter and did not show any significant changes during the growing periods, showing 24.6% in heal- thy first planted field and 19.5% in diseased replanted field respectively. Soil temperature was high- est in July and August and lowest in January and February. The Correlations between environmental influences on microorganisms in soil and its effects on disease development in ginseng field were studied to obtain some useful data for increasing ginseng production and effective preventive measures against the root rot caused by soil-borne plant pathogenic micororganisms in soil. The diseased replanted ginseng fields were selected as the diseased field and the healthy plot in first planted field selected as control in three major Korean ginseng producing areas such as Kumsan, Goesan and Poonggi. The physicochemical characteristics of the soil were analyzed, and micororganism suspectable for root rot ginseng, and other general bacteria were investigated for their population density in various soil conditions. Correlations between soil microbial populations, environmental factors were investigated. The results were as follows: 1. The numbers of fluorescent Pseudomonas spp. were abundant in fall season in healthy first planted field and abundant in hot summer season in the diseased replanted field. In comparison of the relative ratio of the fluorescent pseudomonas spp. in replanted field to healthy field in three areas, the number of fluorescent Pseudomonas spp. were 1.1 to 3.8 times higher in replanted field, and also in ratio of fluorescent Pseudomonas spp. to total bacteria, the numbers of fluorescent Pseudomonas spp. populated in replanted field were 1.3 to 4.9 times higher. Those bacterial cells were highly populated in 10-15 ㎝ depth of rhizosphere of ginseng. 2. The numbers of Erwinia spp. were significantly high in spring and fall in Kumsan and Poonggi. In comparison of the relative ratio of Erwinia spp. in replanted field to healthy field in two areas, the numbers of Erwinia spp. were 2.6 to 4.8 times higher in replanted field, and the numbers of Erwinia spp. relative to total bacteria were 3 to 5 times higher in replanted field than first planted field. In comparison of the relative ratio of Erwinia spp. to fluorescent Pseudomonas spp. in first planted fields of two areas, the range of ratio of two different genus of bacteria was 2.5 : &, which was highly discrepant, on the other hand the ratio in replanted field was 6 : 9, which was very close between the two different genus of bacteria. 3. The numbers of total bacteria in each sampled soil were all high in June through September. There was no significant difference in the vertical distributions of total bacteria according to the depth. There was no significant difference between healthy first planted field and diseased replanted field, in the numbers of total bacteria. 4. The contents of organic matters and phosphate in healthy first planted field were somewhat high, and Phophate / Organic matter ratio and Mg content were high in diseased replanted field. All of the soil samples showed a weak acidic pH of 4.5 to 5.7. Soil moisture content was increased during winter and did not show any significant changes during the growing periods, showing 24.6% in heal- thy first planted field and 19.5% in diseased replanted field respectively. Soil temperature was high- est in July and August and lowest in January and February.

罹病地와 健全地의 人蔘圃 土壤環境에 관한 연구

申鉉成,李敏雄 동국대학교 농림과학연구소 1986 農林科學 論文集 Vol.10 No.-

本 硏究는 土壤微生物과 土壤環境에 의해 發生되는 人蔘 (panax ginseng C.A. Meyer)의 根腐病을 효율적으로 防除하고 또한 이들 病原을 효과적으로 改善, 變化시키므로 人蔘增産을 시도해보고져하였다. 土壤은 우리나라 주요 人蔘栽培地인 錦山, 槐山, 豊基등의 再作罹病圃와 初作健蔘圃場에서 採取하고 이들 土壤의 物理化學的 性質을 조사하였다. 錦山은 土壤型에 있어서 두 土壤條件 사이에 큰 차이가 없고, 土性은 시르트 質壤土 이었다. 槐山은 健全地에서 Ca, Mg의 含量과 砂土量이 적으나, 有機物, 燐酸, 粘土量은 높으며, 健全地 土性은 시르트埴壤土이나 罹病地는 시르트質壤土이었다. 豊基는 健全地에서 K, Mg, 粘土量이 적고 반대로 砂土와 시르트量이 많으나 기타에 성질은차이가 없었다. 3個地域의 罹病地의 健全地 사이에 土壤化學成分의 量을 比로 비교하면 3지역 모두에서 K/유기물, Ca/유기물, Mg/유기물, P_2O_5/유기물, K+Ca/유기물, K+Ca+Mg/유기물의 比는 모두 健全地에 그 量이 높게 함유되었다. 또한 K/Mg, Ca/Mg, P_2O_5/Mg의 比는 錦山과 豊基地域의 健全地에 그 함량이 높으나 塊山은 반대로 罹病地에 그 함량이 높았다. 土壤溫度는 3地域에서 11月 부터 2月 까지 사이에 크게 낮아졌으며 7, 8月 사이에는 높아져서 20-25℃가 되었다. 土壤水分은 큰 차이가 없으나 初作健蔘圃는 24.6%, 罹病地는 19.5%의 平均的인 차이가 있었다. 土壤酸度는 4.5∼5.7 범위에 속하는 弱酸性 土壤이었다. In this study we aimed to obtain an effective control measure against root rot caused by soil microorganisms and soil conditions, and also to increase ginseng (Panax ginseng C.A. Meyer) yield by eliminating the causative factors in that soil environments in three major korean ginseng producing areas such as Gumsan, Goesan, and Poonggi. The Physicochemical characteristics of the soil were analyzed in two different soil conditions like diseased and healthy plot of fields. Soil type of two soil conditions in Gumsan was similar in their properties, and textural class was silt loam. The contents of K, Mg, and clay were low in healthy plot in Poonggi, on the contrary the contents of sands and silt were high in healthy plot of soil. the textural class was wilt loam in two soil. In Goesan area, the contents of Ca, Mg, and sand were low in healthy plot, but the contents of organic matter, P_2O_5, and clay were high in diseased plot. The textural class in diseased plot was silt loam, and on the other hand healthy plot of soil was silt clay loam. In comparison of the ratio of inorganic material between diseased and healthy plot the value of K/Organic matter, Ca/organic matter, Mg/organic matter, P/organic matter, K+Ca/organic matter, and K-Ca-Mg/organic matter were positively high in healthy plot of three soil conditions. The values of K/Mg, Ca/Mg, P/Mg and K+Ca/Mg were also positively high in healthy plot of Gumsan and Poonggi except Goesan. The soil temperature was becoming decreased from November to Feburary and it was highest in July and August ranging 20-25℃. Soil moisture content was increased during the winter and it was not showed any significant changes during the growing season, showing 24.6% in healthy plot and 19.5% in diseased plot by mean number respectively. All the soil samples showed a weakly acidic pH of 4.5 to 5.7.

血液培養에 關한 調査硏究 : Salmonella을 중심으로 on salmonella

申鉉成,李敏雄 동국대학교 대학원 1980 大學院硏究論集-東國大學校 大學院 Vol.10 No.-

Laboratory data on blood cultures were analyzed to determine trends of bacteria isolation isolation in H. Hospital over the period of six years, from Jan, 1974 to Dec. 1979. The results obtained were summarized as follows; 1) pathogenic microorganisms of the 1585 total blood cultures were isolated in 191(12.1%), and salmonella of those in 145(9.2%). 2) The rate of incidence of positive cultures by years showed a tendency of increasing 11.6% in 74 year, 14.3% in 75 year, and 18.8% in 76 year, but decreasing tendency from 77 to 79 years. 3) In decreasing order frequency bacteria isolated from blood culture were salmonella (sal. typhi; 63.9%, Sal. paratyphi A; 12.0%), S. aureus(5.2%), E. Coli(4.7%) and pseudomonas aeruginosa etc., and Gram-bacilli more than Gram+Cocci. 4) Monthly isolation rate of salmonella showed peak in December(17.2%) and July(12.4%) bur lowest in April(0%) and March(2.7%). Seasonal frequency was 32.4% in winter, 31.7% in summer, 26.2% in Fall(autumn) and 9.7% in spring, and winter and summer were significantly different from spring. 5) The age-specific frequency of the salmonella was 41.4% in 20~29 years, 26.2% in 30~39 years while it was only 5.5% in under 10 years and 4.1% in above of 50 years old group. 6) Frequencies in sex showed 86 in Male and 59 in Female. The ratio between male and female was 1.46:1 in whole groups. 7) Widal reaction; a) The results of widal test in 134 patients showed 32.8%(44 patients) in above of 160X and 67.2%(90 patients) in under 160X. b) The age-specific frequency of widal test in above of 160X was 40.9% in 20~29 years, 29.5% in 30~39 years, while it was only 4.5% in 40~49 years old age group. c) The frequency of sex ratio in above of 160X was 30.5%(25/82) in male, 36.5%(19/52) in female. The incidence rate in male showed higher than female, but it was not significantly different from both sexes. 8) The rate of susceptibility to several antibiotics were as follow; a) susceptibility between sal. typhi and sal. paratyphi A was not marked different, and sensitive drug of above 80% was 100% for cephalothin, 97% for minocin, 96.5% for Ampicillin, 94.9% for chloramphenicol and tetracyclin, 92% for carbenicillin and 89.6% for kanamycin. b) E.Coli was sensitive to 100% for gentamicin and kanamycin, 50% for streptomycin and chloram phenicol. c) S. aureus was sensitive to 100% for orberin, 77.8% for Lincocin, 66.7% for chloramphenicol, 60% for streptomycin, and 50% for Erythromycin.

수학교육학과의 교과과정 과목설정에 대한 연구

신현성 한국수학교육학회 1992 수학교육 Vol.31 No.2

Klebsiella-Enterobacter-Serratia의분리 및 항균제에 대한 감수성

신현성,이도현,유희종,황선철 최신의학사 1978 最新醫學 Vol.21 No.11

Enteric bacteria were found to be the most predominantly isolated organisms among the total 1278 clinical isolates during the period of one-year survey. The period seems agreeable among the bacteriology laboratories at where routine clinical specal specimens are handled. The addition of biochemical tests in its identification proctocol at Korea University Hospital made possible to clarify some bacterial species, like E. cloacae, K. ozaenae, C. diversus, and non-chromogenic Serratia species, that fairly overlooked in past. Daily use of references strains along with the commercially available test kits not only brought up the confidence of the identification technique, but also made the laboratory to look into the becterial species. The susceptibility pattern of antimicrobial agents against various organism have not been much changed, though appearance of resistant strains that previously susceptible was noticed in some bacterial species.

忠南一部 地域 供血者의 ABO血液型 分布와 疾患과의 關係硏究 : 肝炎과 梅毒血淸反應 中心으로 on HBsAg and VDRL reaction

申鉉成,李敏雄 동국대학교 대학원 1979 大學院硏究論集-東國大學校 大學院 Vol.9 No.-

This investigation was carried to find out the interrelationship between the distribution of ABO group and prevalence of diseases by blood donor of Chungnam area from Jan. 1977-Sep. 1978. 1. The frequency of ABOblood group in 10388 individuals investigated was as follow; 34.5% for A group 27.4% for B, 26.4% for O and 11.7% for AB group and biochemical race index was 1.18. Frequencies of the genes were 0.266 for gene p, 0.220 for gene q and 0.514 for gene r. 2. The prevalent ratio of HBsAg by blood group were 3.07% for A, 2.78% for B, 2.73% for O and 3.11% for AB group. The difference between the higest group AB and the lowerest group O was not significant. 3. Cardiolipin slide test (VDRL) in which the biological false positive reaction was not considered, showed 0.32%, 0.25%, 0.14% and 0.14% for the B group AB group A group and O group respectively. B and O group was significantly different. 4. The postive ratio of HBsAg was 3.45% for the male and 2.23% for the female. 5. The prevalent of VDRL was 0.1% for male and 0.26% for female, but it was not significantly different. 6. The age-specific prevalent of the HBsAg was found to be 3.3% for 30~39 years, 3.07% for under 20 years, and 1.91% for 20 ~ 29 years of age group, while it was not found in the above of 40 years old group. Especially in the female, there was statistical difference between under 20 years of age and 20~29 years. 7. The age-specific prevalent of VDRL was 0.03% for under 20 years, 0.95% for 20~29 years and 1.65% for 30~39 years age group, while it was not founded in the above of 40 years old group. The positive ratio of VDRL was increased along with the increase 30~39 and 20~29 age group of female was higher then the other groups. 8. The prevalent rates of VDRL among different occupations were as follow; 5.26% for sales clerks, 1.52% for producing worker, repairman and driver, 0.42% for clerk and related worker and 0.14% for unclassified. It was not found among professional technicians and office workers, and those who are engaged in agriculture, animal husbandry, forestry and fishery.

臨床檢體에서 分離된 錄膿菌에 對한 臨床細菌學的 調査硏究

申鉉成,李敏雄 동국대학교 대학원 1981 연구논집 Vol.11 No.

忠南一部 地域 供血者의 ABO血液型 分布와 疾患과의 關係硏究 : 肝炎과 梅毒血淸反應 中心으로

申鉉成,李敏雄 동국대학교 대학원 1979 연구논집 Vol.9 No.-