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1, 2- Dimethylhydrazine 투여후 백서 대장점막 변화에 대한 광학 및 전자현미경적 연구
홍기숙,한운섭,김옥경 梨花女子大學校 醫科大學 醫科學硏究所 1987 EMJ (Ewha medical journal) Vol.10 No.1
The fact that the administration of 1,2-dimethylhydrazine 2Hcl(DMH) to rats by weekly subcutaneous injections causes the development of epithelial tumors of the large intestine has been known. But the axact processes of of early morphologic changes of colonic lesion induced by DMH treatment in the rats have not been fully testified as yet. The objective of the dissertation research is to examine early morphologic changes of colonic lesion induced by DMH treatment in the rats. Total seventy cases including 14 control rats of Sprague Dawley male rats were analyzed by histopathologic changes. One is 20mg/kg/wk DMH treated rat group and the other is 40mg/kg/wk DMH treated rat group. DMH was injected s.c. at the start of each treatment weekly during 20 weeks, and the animals were killed at 1, 3, 5, 6, 7, 9, 12, 14, 17 or 20 weeks both in 20mg/kg/wk DMH treated group and in 40mg/kg/wk DMH treated group. The following results were obtained: Hyperplasia and mitosis of the crypt are presented in early phase of DMH treatment. Cellular dysplasia, carcinoma in situ, and invasive carcinoma are shown in later phase of DMH treatment. The same changes as the above are also identified in electron microscopic findings. The more doses of DMH are injected to rats, the more evident these findings are presented. The alkaline phosphatase activity in glandular epithelial cells are observed in cellular dysplasia and neoplastic changes. The alkaline phosphatase may be considered as tumor marker.
방광에 발생된 Malignant Mixed Mesodermal Tumor : 증례보고 및 문헌고찰 A Case Report and Literature Review
홍기숙,한운섭,김옥경 梨花女子大學校 醫科大學 醫科學硏究所 1981 EMJ (Ewha medical journal) Vol.4 No.3
A malignant mixed mesodermal tumor of the bladder from 70-year-old woman is presented. Malignant mixed mesodermal tumor is a designation applied to carcinosarcoma characterized by the presence of heterologous mesenchymal elements. The tumor tends to be large, bulky, intraluminal tumors that grows rapidly and infiltrates widely. The tumor occurs predominantly in elderly men, and aggresive surgical treatment is indicated because of the poor prognosis. Histologically this neoplasm was composed of epithelial components of transitional and squamous cell carcinoma, and mesnechymal components of fibrosarcoma, chondrosarcoma, and osteogenic sarcoma.
홍기숙,김옥경 梨花女子大學校 醫科大學 醫科學硏究所 1991 EMJ (Ewha medical journal) Vol.14 No.3
Three new developed enzyme immunoassays(EIA) for urinary pregnancy tests were compared with the three older latex agglutination assays(LATEX) in the urine samples of 138 patients. The examined kits included EIA (Testpack hCG-Urine. Tandem ICON Ⅱ hCG Urine, Target hCG) and direct LATEX (Betatex direct, Pregnancy Quick test, Synchron hCG). The results were as follows: 1) The coincidence rate between EIA and direct LATEX is 91%. The coincidence rate between EIA and radioimmunoassay(RIA) is 100%. 2) The range of the discrepancy between EIA and LATEX is 79㎖U/㎖ to 855㎖U/㎖. 3) When the EIA is considered to be the standard method. the sensitivity and specificity of Latex are 78% and 100%. 4) The hCG of the urine specimen which were found to be negative by EIA and LATEX is below 50㎖U/㎖. 5) There is no false positive result in proteinuria(2+) and bloody urine(1+) in the EIA and Latex. We conclude EIA is the nost sensitive procedure(lowest detection limits)for qualitatively detecting hCG in urine samples secreted by early pregnancy.
1, 2-Dimethylhydrazine으로 대장암이 유발된 백서의 혈청 및 대장 alkaline phosphatase 활성도에 관한 실험적 연구
洪起淑 梨花女子大學校 韓國生活科學硏究院 1990 韓國生活科學硏究院 論叢 Vol.45 No.-
1, 2-dimethylhydrazine(DMH)투여로 백서대장 점막의 암발생을 유도한후 혈청 및 대장점막내 alkaline phosphatase(ALP)활성도를 측정한바 다음과 같은 결론을 얻었다. 1. DMH투여 20주동안 대장암의 발생율은 21.7%였고 간장으로의 전이는 없었다. 2. 혈청내 총 ALP활성은 DMH투여군에서 증가되었고, Vitamin A를 같이 투여한 군에서 감소되었다. 3. 혈청내 ALT는 DMH투여군에서 증가추세를 보였으나 AST, r-GT는 의의가 없었다. 4. 혈청의 열저해검사시 DMH투여군들에서 높은 경향을 보였고, 10mMol phenylalanine 저해시 ALP-2 band가 감소되는 것을 관찰하였다. 5. 대장점막의 ALP염색상 이형성 및 침습암이 있는 경우 ALP염색상이 증가되는 경향을 보였다. 이상의 결과로 혈청내 ALP증가가 대장암의 표식자로 보기 보다는 대장점막 및 간장의 염증반응에 의한 증가로 사료되었다. An alkaline phosphatase activity in serum, colonic mucosa and colonic carcinoma was studied in 76 rats which were induced colonic carcinogenesis by the 1, 2-dimethylhydrazine. Total serum alkaline phosphatase was more increased in dimethylhydrazine treated group than in normal control rats. Liver metastases was not present during 20 weeks 1, 2-dimethylhydrazine treatment. Serum alanine amino transferase was increased in DMH treated group, but aspartate amino transferase and gamma glutamyl transferase activity were not significant. After heat inactivation, the remained serum alkaline phosphatase was more increased in 1, 2-dimethylhydrazine treated rats. After phenylalanine treatment, the electrophoresis of alkaline phosphatase show decreased in alkaline phosphatase 2 band. The alkaline phosphatase activity in glandular epithelial cells were observed in cellular dysplasia and neoplastic change. All thses findings show the serum alkaline phosphatase activity do not appear to be influenced by the enzyme production in cancer tissue.
단일클론항체를 이용한 면역진단법의 연구 : Flow Cytometry에 의한 세표표면 표현형 검사 Flow Cytometry for Cell Surface Phenotyping
홍기숙 梨花女子大學校 醫科大學 醫科學硏究所 1992 EMJ (Ewha medical journal) Vol.15 No.1
With the development of monoclonal antibodies. they have various utilities for in-vitro diagnosis in differentiating tumor cell type, and for therapeutic purposes. I experimented the reaction of many known and new monoclonal antibodies using flow cytometry. The results are as follows: 1) There were 18 cases of B-lineage Acute lymphoblastic leukemia(ALL)(64%), 8 cases of T-lineage ALL(28%), 1 case of B-myloid ALL(4%) and 1 case of B cell All(4%) in 28 cases of ALL. 2) The reactions with anti-CD9, CD10, CD19, CD24, CD34, CD72 and HLA-DR were positive in most cases of non-T-All, and the reaction with ani-CD13, CD33, CD40 were relatively positive in a few cases of non-T-ALL. 3) The reaction with anti-CD24, CD19, CD20, CD72 and HLA-DR was strong positive in 1 case of Hairy cell leukemia(HCL). 4) There were 17 cases of B-Chronic lymphoytic leukemia(CLL)(71%), and 4 cases of T-CLL(17%) in 24 cases of CLL. 5) There were 8 cases of B-non Hodgkin's malignant lymphoma(NHL)(57%), and 4 cases of T-NHL(29%) in cases of MHL. 6) There were positive reaction with anti-CD2 in normal bone marrow, fetal bone marrow with anti-CD19, tonsil with anti-CD4, anti-CD19, and thymus with anti-CD2. Immunophenotype with panel of monoclonal antibodies using flow cytometry is very helpful to take diagnosis and treatment for hematologic malignancies.