結核患者에서 分離된 非定型抗酸菌의 微生物學的 硏究

崔大卿,張明雄,朴鼎圭 충남대학교 의과대학 지역사회의학연구소 1980 충남의대잡지 Vol.7 No.2

Attentions have been devoted to the biological and chemotherapeutical studies on the acid fast bacilli isolated from 220 patients who were admitted to the National Kongju Hospital from March to June in 1980. The results were summerized as follows: 1. The sex distribution of 220 patients were 152 males and 68 females. 2. Seventy (31.8%) strains of acid fast bacilli were isolated from 220 patients sputa. 3. Culture were considerablely more sensitive than direct smear in detecting the presence of mycobacteria. 78.6 percent of all culture positive were positive in the direct smear. 4. When the 70 isolated strains classified by Runyon groups, 6 strains (8.5%) were belong to the rapid growers and the rest 64 strains (91.5%) were belong to the M. tuberculosis. 5. In drug sensistivity test, drug resistant strains were found 16. 6 percent of isoniazid(1㎍/ml), 37.0 percent of streptomycin (10㎍/ml) and 40.3 percent of ethambutol (2㎍/ml). From the above results, it was suggested that the majorities of tuberculosis patients were caused by the infection of M. tuberculosis, that strains were resistant to the anti-tuberculosis drugs. It is mainly caused by the increasing occurrence of resistant bacilli which is attributable to inadequate drug medication. The correct chemotherapy based on reliable sensitivity test. It prevents the occurence of resistant bacilli, relapses and stops the progress of the diseases.

건강인에서 결핵균 30-kDa항원이 말초혈액 임파구증식에 미치는 영향

최대경,고석신,김화중,조은경,백태현,신철식 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

T lymphocytes are thought to play a central role in cell mediated immune response. To Study the T lymphocyte proliferative response to 30-kDa antigen purified from Mycobacterium tuberculosis H37Rv, peripheral blood lymphocytes isolated from healthy subjects were stimulated with the 30kDa antigen, crude antigen or PHA. Healthy subjects were divided into three groups so that PPD(+), PPD(±) and PPD(-). The proliferations of lymphocytes were measured by the incroporation of ^3H-thyrnidine. The results were summarized as follows: 1. For the PPD(+) person, at concentration of < 1 ㎍/ml, the proliferative response to 30-kDa antigen resulted in a mild reduction but crude antigen showed relatively rapid reduction. For the PPD(-) person, decreasing the concentration of the both antigens showed rapid reduction of proliferation 2. The lymphocytes proliferations to 30-kDa antigen and crude antigen were significantly increased in PPD(+) group when compared to those in PPD(-) group, but PHA response was no significant difference. 3. The sensitivity and specificity of lymphocyte proliferation assay to 30-kDa antigen and crude antigen when compared with skin test results were 92.3%, 100% and 76.9%, 50%, respectively.

抗生物質 : 化學療法과 微生物學的 問題點

崔大卿 中央醫學社 1974 中央醫學 Vol.26 No.2

Colchicine이 細菌增殖에 미치는 影響

崔大卿,張明雄,朴鼎圭 충남대학교 의과대학 지역사회의학연구소 1981 충남의대잡지 Vol.8 No.2

Attentions have been devoted to the effects of colchicine on the growth and biochemical characteristics of Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (phage type 29) in BHI broth containing 1 and 5 mg per ml of colchicine. The results were summarized as follows: 1. Growth of Bacillus subtillis was decreased after 12 hours exposure to the 1 mg per ml of colchicine but growth of Staphylococcus aureus was not influenced to it. 2. Growth of Bacillus subtilis was remarkably decreased after 8 hours exposure to the 5mg per ml of colchicine but Staphylococcus aureus also remarkably decreased after 2 hours exposure to it. 3. One and 5mg per ml of colchicine was not influenced to the biochemical characteristics of Bacillus subtillis and Staphylococcus aureus. As above the result, it was suggested that 1 and 5mg per ml of colchicine was inhibited to growth of some bacteria but its effects was different to the bacterial species or strains. It was not influenced to the biochemical characteristics of some kind of bacteria.

다분절 척추 유합술의 유합 분절 범위가 요추에 미치는 생체역학적 영향

최대경,김경수,김윤혁 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.4-4

CFD 해석을 이용한 누설 포집 시스템 해석 방법 수립 및 평가

최대경,박원만,어동진,권태순,최청열 한국유체기계학회 2023 한국유체기계학회 논문집 Vol.26 No.5

Suppose a small amount of leakage exceeds the limit of the operating conditions of a nuclear power plant. In that case, losses due to the nuclear power plant shutdown may occur, or accidents due to leakage may occur. Consequently, there has been a need to develop a system capable of quickly detecting even small amounts of leakage, and research has been conducted to address this need. To develop such a system, it is necessary to understand the thermal-hydraulic characteristics of the system. This study established a CFD-based evaluation process for evaluating leak detection systems. A numerical analysis model was used to simulate leakage, and a two-step CFD analysis was performed to assess its applicability. In the first step, a study of the leakage behavior in the insulation material was conducted, and the effect of the gap between the pipe insulation material and the outer cover was evaluated. In the second step, the collection loop was analyzed using the results of the leakage behavior analysis in the insulation as an input condition. The results of this study were used to construct the collection loop experimental device and will help in understanding the heat flow characteristics in the collection loop.

결핵균체 단백항원에 대한 항결핵균 IgG 항체에 관한 연구

최대경,백태현,권지순,심운택,이동배 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

In order to study the level of IgG antibody binding to sonicate antigen of Mycobacteriumn tuberculosis H_34Rv for ammonium sulfate 30∼40 fraction in sera isolated from 209 patients and. 135 controls were measured by plastic tube enzyme-linked immunosorbent assay (ELISA). The levels of IgG antibody binding to Mycobacterium tuberculosis H_37Rv antigen in sera from all patients and controls were measured after absorption of 1 : 400 diluted sera with ,Wycobacteriuez bovis and dMlycobacterium Kansasii for ammonium sulfate 30∼40 fraction. The authors conducted the survey from August 1984 to January 1985. The results obtained were as follows : 1. At the serum dilution of 1:400, the mean ELISA values of IgG antibody to Mycobacterium tuberculosis H_37Rv after absorption with Mycobacterium kansasii antigen from far advanced patients and control were 0.8666±0.4903 and 0.2058±0.1353, respectively. 2. The mean ELISA value of IgG antibody to Mycobacterium tuberculosis H_37Rv afer absorption with Mycobacterium kansasii antigen from moderate patients was 0.6488±0.3918, the ratio of ammonium sulfate fraction 30∼40 in sera from patients and controls was 3.153. 3. The mean ELISA value of IgG antibody to Mycobacterium tuberculosis H_37Rv after absorption with Mycobacterium kansasii antigen from minimal patients was 0.4205±0.3155, the ratio of ammonium sulfate fraction 30∼40 in sera from patients and controls was 2.043.

병원내에서 분리된 다제 내성 포도구균에 관한 연구

최대경,장명웅,박정규,김세겸,나상연,민병무 충남대학교 의과대학 지역사회의학연구소 1980 충남의대잡지 Vol.7 No.1

In order to know the multiple antibiotic resistance of isolated Staphylococci in the air of various places of Chungnam National University Hospital, 2, 679 bacterial strains were collected at the department of Microbiology, Chungnam National University School of Medicine, during July, 1979. The isolation rate, biochemical character and antibiotic resistance of isolated Staphylococci were studied. The results were summerized as follows: 1. The isolation rate of Staphylococci in the Hospital air were 16.1%(432 strains out of total 2,679 strains). 2. Coagulase activity of the isolated Staphylococci were positive in 314 strains out of 432 strains isolated(72.7%). Among of coagulase positive strains, 126 strains fermented mannitol(29.2%). 3. In the coagulase positive and mannitol fermenter, the isolation rate of resistant strains by one antibiotic was 69.1% (C.P.), 57.9% (L.M.), 35.7% (C.C.), 31.7% (S.M.), 22.2%(K.M.), 19.8%(A.P.), 11.0%(C.D.), and 18.3%(G.M.). 4. The multiple antibiotic resistance of Staphylococci isolated were resistant to 9 anti biotics(1.4%), 8 antibiotics(6.5%), 7 antibiotics(4.6%), 6 antibiotics(5.1%), 5 antibiotics(5.3%), 4 antibiotics(8.6%) 3 antibiotics(14.1%), 2 antibiotics(15.0%) and all antibiotics sensitive(19.9%)

癩患者로 부터 癩菌檢出 方法의 改善(Ⅱ)

崔大卿,張明雄,朴鼎圭 충남대학교 의과대학 지역사회의학연구소 1982 충남의대잡지 Vol.9 No.1

For the improvement of detection of leprosy bacilli from patient, author's attention of the bacterial detection ratio have been devoted to compared the skin smear method with the nuclepore filter method from 200 patients who were registrated in Chungnam Skin Clinic of Korean Leprosy Association. One hundred patients out of 200 registered were positive in bacterial detection, the others were negative. For the dispersion of bacterial globi in nuclepore filter method, the skin scrapped materials were treated with 0.025% trypsin and 0. 002 % tween 80 for 30 minutes at 37℃ incubator. The efficiency of bacterial detection between the nuclepore 13 mm diameter of the filter and the 25 mm filter were investigated in the broth cultured M. fortuitum. The cultured M. fortuitum was transferedl ml into each 10 tubes, and then was carried out ten-fold dilution, respectively. The specimen for comparative counting of acid fast bacilli were made on 10^-4 dilution in using 25 mm diameter of filter and 10^-5 dilution in using 13 mm diameter of filter and cultural method. The results were summarized as follows: 1. The ratio of bacterial detection by the skin smear and nuclepore filter methods from 100 patients who were registrated with bacterial detection positive cases were not observed any difference, in all cases were detected leprosy bacilli with both methods. 2. In 100 patients who were registrated with bacterial detection negative, 19 patients were detected leprosy bacilli by the skin smear method and 33 patients were detected leprosy bacilli by the nuclepore filter method. The ratio of bacterial detection by two methods were observed statistically significant difference. (0.01<p<O. 05). 3. Between bacterial indices by skin smear and bacterial numbers of nuclepore filter methods were observed perfect positive corelation. 4. There was close corelation between 25 mm filter and the culture method, however, 13 mm filter was more higher than other two methods. From the above results, it was suggested that the nuclepore filter method with well digesting specimens with 0.025% trypsin and 0.002% tween 80 would be more accurate and higher detection ratio of M. leprae than skin smear method. The nuclepore 13mm filter was more efficient to detection of acid fast bacilli than 25 mm filter.

癩菌의 實驗的 硏究

崔大卿 세계평화교수협의회 1976 廣場 Vol.33 No.-