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오현우,박호용,Oh, Hyun-Woo,Park, Ho-Yong 한국현미경학회 2004 Applied microscopy Vol.34 No.4
초파리 돌연변이를 이용한 신경연접에서의 신경충격의 전달을 알아보기 위하여 배양한 초파리 배자 신경세포의 신경연접 미세구조를 관찰하여 분석하였다. 배양된 Wild-type 초파리 배자 신경세포의 신경연접(synapse)은 신경연접간극(synaptic cleft)에 의해 구분되면서 평행하게 뻗어있는 신경연접전 돌기(presynaptic area)의 세포막과 신경연접후 세포(postsynaptic cell)의 세포막 구조에 의해서 확인하였다. Presynaptic active zones과 postsynaptic densities는 각 세포막부분의 전자밀도에 의해 구분하였다. 특히 두 개의 세포막이 서로 근접하여 있으면서, 하나 또는 그 이상의 전자밀도가 높은 presynaptc densities 를 가지고 있고 그 주위에 투명한 신경연접소포들(clear core synaptic vesicles)이 모여있을 경우 이를 신경연접전 돌기로 보았다. 신경연접전 돌기에는 평균 $35.1{\pm}1.44$ nm 직경의 작고 투명한 신경연접소포들이 모여있었다. 신경연접소포들 중 일부는 세포막이나 세포막의 전자밀도가 높은 부분에 직접 접촉하고 있었는데 이를 신경전달물질이 방출되기 직전인 morphologically docked vesicles로 보았다. 이외에도 신경연접전 돌기에서는 내부가 전자밀도가 높은 물질로 채워져 있고 직경이 큰 dense core 신경연접소포들도 관찰할 수 있었다. To identify the structural basis of mutations that affect synaptic transmission we have begun quantitative ultrastructural descriptions of synapses in cultured Drosophila embryonic neurons. In wild-type cultures, synapses are distinguished by the parallel arrangement of a thickened pre- and post synaptic membrane separated by a synaptic cleft. The presynaptic active zones and postsynaptic densities are defined by electron dense material close to the membrane. Presynaptic regions are also characterized by the presence of one or more electron dense regions, presynaptic densities, around which a variable number of small, clear core synaptic vesicles (mean $35.1{\pm}1.44$ nm in diameter) are clustered. Subsets of these vesicles are in direct contact with either the presynaptic density or the membrane and are considered morphologically docked. A small number of larger, dense core vesicles are also observed in most presynaptic profiles.
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오현우 서울大學校人文大學 1976 人文論叢 Vol.1 No.-
Si le schema dramatique du Rouge et le Noir est tres voisin de celui de l'affaire Berthet, le heros du roman est peut-etre plus proche de l'affaire de Lafargue, tel, du moins, que Stendhal a voulu voir ce personnage. On doit considerer que l'argument remanesque du Rouge et le Noir puise a desx sources diverses. Nous avons considere jusqu's a maintenant des faits qui, quoique oublies, furent de notoriete publique et qui pouvaient donc etre consideres comme des sources proprement historiques du Rouge et le Noir. Mais le roman est riche, en outre de toute une experience particuliere, qui est celle du remancier. Retrouver les elements epars de cette experience, tel est l'objet de notre etude, que se referera souvent a des realites obscures, mais proches de Stendhal, et qui fournira ainsi de nouvelles donnees pour l'enquete de genese. Parmi ces elements, les uns sont etrangers a la vie personnelle de l'ecrivain, qui s'inspire parfois de teztes anterieurs, d'autres, qu'il s'agisse de paysages ou de personnages, sont etroitement meles a ses aventures; beaucoup lui sont consubstantiels au point de nous imposer la necessite d'un parallele entre son heros et lui-meme. Ls passion essentielle, c'est naturellement l'amour. Stendhal en distingue quatre formes. Une des theses classiques de l'amour est celle de la "cristallisation des sentiments". L'idee de Stendhal, c'est que l'amour, sentiment subjectif, depend plus de celui que aime que de la personne qu'il aime. Donc, subjectivite de la vie amoureuse. L'amour n'est qu'une forme de folie, puisque, dans l'amour, nous voyons les etres autrement qu'ils ne sont, mais, pense Stendhal, c'est une folie delicieuse et c'est ha seule qui rende la vie digne d'etre vecue. Ces idees, Stendhal a essaye de les exprimer a l'aide de Julien Sorel. Julien, des qu'il a revu Madame Reanl parfaitement simple et naturelle, qui jamais n'enfle ses sentiments, a su qu'il n'aimait qu'elle. Enfin, il comprend. Le seul vrai bonheur est dans ces instants de tendresse, passes a dire des riens entrecoupes de baisers. L'amour vrai, ce parfait accord avec soi-mome qui est l'effet de la sincerite, voila les seuls biens dont l'homme puisse etre sur.
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Hydrofluoric acid 용액을 이용한 유리 커버글라스에 배양된 신경세포의 전자현미경 시료제작법
오현우,박호용,Oh, Hyun-Woo,Park, Ho-Yong 한국현미경학회 2005 Applied microscopy Vol.35 No.4
신경연접 구조 관찰을 위한 방법으로 hydrofluoric acid 용액을 이용하여 유리 커버글라스 위에 배양된 신경세포의 전자현미경 관찰을 위한 편리한 시료제작법을 제시하였다. 배양된 신경세포의 TEM관찰을 위한 전체적인 시편제작 방법은 일반적인 전자현미경 시편 준비법과 큰 차이가 없으나 시료 블록을 제작한 후 유리 커버글라스를 효과적으로 제거하는 것이 가장 큰 차이점이다. 유리 커버글라스의 제거를 위해 열중합 반응이 끝난 시료 블록은 유리 커버글라스를 완전히 노출시켜 48% hydrofluoric acid 용액이 들어있는 플라스틱 비이커에 넣어 $5{\sim}10$분간 처리하여 유리 커버글라스를 완전히 제거한 후, 흐르는 물로 충분히 세척하고 상온에서 건조하여 사용하였다. 유리 커버글라스가 제거된 시료 블록은 실체 현미경을 이용하여 관찰하고자 하는 부위를 선정한 후 절삭하여 절편을 제작하였다. Cultured neurons from Drosophila brain on a glass coverslip to understand the structural basis of synapse were prepared for TEM observations. Neurons on a coverslip were fixed, dehydrated and embedded in Epon without separating from coverslip. After polymerization, the block was placed in 49% hydrofluoric acid to remove the coverslip. The block was examined under a light microscope to select exact neurons, then trimmed and sectioned for TEM observation.
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오현우 서울大學校 人文學硏究所 1981 人文論叢 Vol.7 No.-
Un courant thematique s'immisce dans la critique litteraire. Nous le savons notamment depuis Bachelard, Poulet, Rousset et Starobinski, auxquels s'apparente encore Proust au sens large de la thematique. Dans un passage d' A la recherche du temps perdu, il a remarque: "mais vous verriez dans Stendhal un certain sentiment de l'altitude se liant a la vie spirituelle: le lieu eleve ou Julien Sorel est prisonnier, la tour au haut de laquelle est enferme Fabrice, le colcher ou l'abbe Blanes s'occupe d'astrologie et d'ou Fabrice jette un si beau coup d'oeil." Ainsi notre etude se permet-elle de savoir si cette remarque preliminaire pourrait etre justifiee meme par d'autres critiques thematiques de l'oeuvre de Stendhal.
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다중 팔렛 시스템에 사용되는 서보 모터의 제어에 관한 연구
오현우 대한임베디드공학회 2019 대한임베디드공학회논문지 Vol.14 No.6
Multi-axis servo systems are widely used in various fields such as industiral systems for improving production efficiency, robotics and complex systems where many mechanical devices and sensor systems are connected. Such a servo system requires that the servo control technique to realize the synchronization of the drive shaft in the steady state and transient conditions and to control so as to follow the target track in order to improve product precision and production efficiency. In addition, embedded type hardware is required for smooth control of the entire multi-axis system. Therefore, this paper uses hardware based on FPGA which is widely used in digital signal processing field and various control system because hardware design change is easy and parallel processing is possible. In addition, Labview based servo motor control program was studied that can control the servo motor by ensuring the performance and flexibility of the FPGA and follow the target trajectory according to various speed processing and accurate timing synchronization.
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SERVPERF와 고객여정지도를 활용한 의료서비스 개선 요건 파악
오현우,함동한 대한안전경영과학회 2017 대한안전경영과학회지 Vol.19 No.4
This paper is aimed at proposing a new approach to connecting the measurements of customer satisfaction on healthcare services with the prioritized identification of healthcare service processes to be improved. As customers’ requirements for healthcare services have become too diverse and healthcare service systems have been increasingly complex, there has been growing interest in the customer-oriented evaluation of healthcare service quality and the systematic improvement of healthcare service processes. Most of the previous studies on service quality evaluation are based on SERVQUAL model. However, because of the unique characteristics and constraints inherent in healthcare service systems, it has been reported that SERVQUAL would be inadequate to be applied to healthcare service systems. As an alternative, SERVPERF has recently been widely used in the evaluation of healthcare service quality. However, there is a lack of studies on how to use the measurements of healthcare service quality systematically to improve service functions and processes. With this issue in mind, we firstly measured the customer-perceived satisfaction on the healthcare service quality from the six dimensions based on SERVPERF. Then we identified the relationships between the subjective measurements and healthcare service processes through brainstorming and expert interview. By using the relationships, we developed a customer journey map in healthcare services that visually describe the interaction between customers and healthcare service systems. The developed customer journey map would help service designers easily identify a healthcare service process that needs to be improved with priority. It is expected that the design improvement process proposed in this study would be a useful method for enhancing the quality of healthcare services.
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