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Ethephon과 Gibbrellin 處理가 오이의 性發現과 이에 따른 蛋白質含量, Peroxidase活性 및 Isoperoxidase Pattern에 미치는 影響
具禹書,金暎來 충남대학교 농업과학연구소 1985 農業技術硏究報告 Vol.12 No.1
This experiment was conducted to study sex expression and subsequent changes in protein and peroxidase after three cultivars of cucumber plants were treated with ethephon and gibberellin. The three cultivars of cucumber used in this study included 'Sayeup' (monoecious type), 'Sinrokdadaki' (gynoecious type), and 'Seonghowon' (intermediate type). The ethephon at 250ppm and gibberellin at 100 ppm were treated at the 2-leaf and 4-leaf stages, and subsequent sex expression and changes in protein contents, peroxidase activities and isoperoxidase banding patterns by disc polyacrylamide gel electrophoresis were studied. The results can be summarized as follows: 1. Ethephon treatment slightly increased number of pistillate flowers and significantly decreased number of staminate flowers in the three cultivars, while gibberellin treatment significantly increased number of staminate flowers in both gynoecious 'Sinrokdadaki' and intermediate 'Seonghowon' and did not increase number of staminate flowers in monecious 'Sayeup' 2. There were some differences among three cultivars in protein contents, protein banding and isoperoxidase banding patterns of seeds and germinating seeds, However, it was not obvious to differentiate monoecious from gynoecious cultlvars by these characters. 3. Protein contents in the leaves and stem apex after ethephon and gibberellin treatment increased gradually at the 2-leaf stage, but decreased at the 4-leaf stage. Protein contents in stern apex at the 4-leaf stage without treatment were much higher in 'Sinrokdadaki' and `Seonghowon' than in monoecious 'Sayeup'. Protein contents in the stem apex at the 4-leaf stage were increased in the ethephon-treated monoecious 'Sayeup' and decreased in 'Sinrokdadaki' and 'Seonghowon' compared with untreated plants. 4. Peroxidase activities in the leaves and stem apex gradually decreased at the 2-leaf stage, but increased at the 4-leaf stage. Peroxidase activities in stem apex at the 4-leaf stage were significantly increased by ethephon treatment. 5. The number of protein bands in the three cuitivars after treatment gradually decreased in leaves and stem apex at the 2-leaf stage, but increased in the 4-leaf stage. The protein banding pat terns of stern apex of the ethephon- treated monoecious ' Sayeup' at the 4- leaf stage were gradually shifted to the banding
오이의 性發現에 미치는 Ethephon과 Gibberellin의 自葉期 處理 效果
鄭淳宰,具禹書 東亞大學校 1993 東亞論叢 Vol.30 No.1
오이는 性發現에 관한 基礎資料를 얻고져 飛節成種인 四葉과 節成種인 신록다다기, 그 中間形인 聖護院을 공사하여 種子와 崔芽종자의 蛋白質含量의 品種간 차이와 子葉展開期의 ethephon(250ppm)과 Gibberellin(100ppm)處理에 의한 蛋白質 含量과 peroxidase活性의 變化, 蛋白質 banding pattern 및 perosidase isoenzyme의 banding pteern의 變化 關係를 調査하여 다음과 같은 結果를 얻었다. 1) 種子와 崔牙種子인 蛋白質含量, 蛋白質 banding pattern 및 peroxidase의 banding pattern에서 飛節成과 節成品種간에 뚜렷한 차이를 볼 수 없었다. 2) 子葉期의 蛋白質 含量과 蛋白質 banding pattern에서는 3品種 모두 ethephon處理區에서 蛋白質含量이 높았고, 蛋白質 banding pattern에서는 處理 後 日數가 지남에 따라 變異를 보이다가 處理後 5日째 四葉의 ethephon 處理區의 Rf 0.33-0.34의 位置에 强度높은 band가 形成되었으며, 聖護院, 신록다다기의 無處理區에서 形成된 band의 位置와 强度가 同一하였다. 3) 子葉期의 peroxidase 活性 및 peroxidase banding pattern에서는 ethephon處理區에서 3品種 모두 peroxidase activity가 높게 나타났으며, ethephon 處理後 1일째는 3品種 모두 거의 같은 band의 樣相을 나타내었고, 處理 後 3일째는 變化의 樣相을 보이다가 處理後 5일째는 四葉의 ethephon 處理區와 聖護院, 신록다다기의 control區가 거의 같은 banding pattern을 나타내었다. This experiment was conducted to obtain the basic information the patterns of change in protein contents, peroxidase activity and of sex expression between three cucumber varieties(monoecious type;Sayeupl gynoecious type; Sinrokdadaki, intermediate type; Seonghowon) by treating ethephon with 250ppm and gibberellin with 100ppm at the maturing stage of their cotyledons. The patterns of changes in protein contents and peroxidase activity were analyzed using disk polyacrylamide gel electrophoresis and the results are summarized as follows. 1. No difference was found in changes of the protein contents, band pattern of peroxidase and iso peroxidase activities between mature seeds and germinating seeds of Sayeup(monoecious type) and Sinrokdadaki(gynoecious type). 2. High protein contents were obtained in cotyledons of all varieties treated with ethephon, and a variety of band patterns of protein isolated were shown on the gel during the initial stages after ethephon treatment. After 5 days of ethephon treatment, however, a strong band was observed at the Rf value of 0.33-0.34 on the gel analyzed from the proteins isolated from Sayeup cotyledons with etyephon treatment and the band intensity of their proteins was similar to ones from Seonghowonand Sinrokdadaki with no ethephon treatment. 3. As a result of gel analysis, high activity and strong band of peroxidase isolated from ethephon treated carieties was examined. Similar band patterns of proteins isolated were observed in the cotyledons of all carieties used after one day of ethephon treatment. After 3 days of ethephon treatment, all varieties showed various band patterns, while after 5 days, the band pattern of proteins isolated from ethephon treated Sayeup similar one of proteins from both Seonghowon and Sinrokdadaki with no ethephon.
鄭淳宰,具禹書,鄭景泰 東亞大學校 大學院 1992 大學院論文集 Vol.17 No.-
Experiments wer conducted to investigate of rootstocksk on the growth, yield and quality of oriental melon cultivars and to find out problems associated with successful growing of grafted seedlings. Major results can be summarized as follows. 1. The percentage of healthy plants, sintozwa and chamtozwa were 100, while hongtozwa and chambak were less than 11.8. 2. The growth of oriental melon was promoted by non-grafted and sintozwa root stock. 3. Soluble solid contents of non-grafted and chamtozwa rootstock were hight than sintozwa rootstock. 4. Glucose and Fructose contents of sintozwa rootstock was hihter than chamtozwa rootstock. Whereas, sucrose contents of non-grafted was hiher than sintozwa, chamtozwa rootstock. 5. Grafting onto sintozwa rootstock increased the contents of most mineral element except Ca in the leaves.
박흥식,오주성,구우서,정순재 東亞大學校附設 農業生命科學硏究所 1999 農業生命資援硏究 Vol.8 No.1
Recently government and general public have realized that recent agricultural practices have caused problems on soil properties, natural ecological systems, and environmental pollution also, due to the rapid economic growth, trade policy has to be advanced, import restrictions to protect domestic agricultural products are getting difficult in the international society. Trade liberalization will general marketing competition of our domestic agricultural products, and up with surplus problem. Now, environmental protectionism is getting popular, and organic farming is first initiated with main concern and submitted the national. proposal in the convention meeting of all agricultural societies. The aim of this paper is trying to report and discuss with you about present situation of organic farming research progress, on-going research, production aspects and problems or constrains in Korea.