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Lim, Wang-Jin,Choi, Kyung-Min,Hwang, Se Young 한국미생물 · 생명공학회 1993 Journal of microbiology and biotechnology Vol.3 No.4
A novel system has been developed to produce δ-aminolevulinic acid (ALA) using the intact cells of late logarithmic phase of Rhodocyclus gelatinosus KUP-74. The system was shown optimum yield of extracellular ALA under a condition of anaerobic light irradiation (4 Klux) at 30℃ with no variation in cell mass. The rate of extracellular ALA formation was stimulated by low doses of either C_4 or C_5 ALA biosynthetic precursors, where 5 mM (C_4) and 3 mM (C_5) of each precursors were appeared to generate the maximum rates of 3.3 and 4.0 nmoles of ALA per 0.35 ㎎ cells per hr, respectively. Half-life of the system was 10 hr in a sense of an ability of portage transport of L-glutamate, and sequential dose of this compound was resulted in promising recovery of the ALA.
Lim, Wang Jin 한국농화학회 1991 Applied Biological Chemistry (Appl Biol Chem) Vol.34 No.4
Six stains of methylotrophic yeast were examined for production of L-methionine-enriched cells. Candida boidinii (Kloeckera sp.) No. 2201, which accumulated 0.54 ㎎/g-dry cell weight (DCW) of free L-methionine (pool methionine), was selected as the parental strain for breeding L-methionine-enriched mutants. Ethionine-resistant mutants derived from the strain by W irradiation. A mutant strain, E500-78, which was resistant to 500㎍/㎖ of DL-ethionine, accumulated 6.02㎎/g-DCW of pool methionine. The culture conditions for mutannt strain E500-78 to increase pool methionine accumulation were optimized. The mutant strain accumulated 8.80 ㎎/g-DCW of pool methionine and contained 16.02 ㎎/g-DCW total methionine. L-methionine-enriched cells production of mutant strain E50078 was greatly improved by the control of pH and by feeding of methanol and other medium components during cultivation in a jar fermentor. Under the optimal conditions, 38.5g (as dry weight) of cells and 282 ㎎ of pool methionine per ℓ of culture broth were obtained after 11 days of cultivation. With limited methanol in continuous cultivation, pool methionine productivity reached a maximum value of 1.14 ㎎/ℓ/h at a dilution rate of 0.05 h^(-1). The effects of an ethionine-resistant mutation in a methylotrophic yeast, Candida boidinii, were studied. In mutant strain E500-78 (ethionine-resistant), SAM synthetase activity was low and was only slightly repressed by L-methionine. Formyltetrahydrofolate synthetase and serine hydroxymethyltransferase were involved in synthesis of the methyl group of L-methionine. The activities of the methyl group transferring enzymes and homocysteine transmethylation were repressed by L-methionine in the wild type strain, but not in the mutant. The activities of the methyl group transferring enzymes were markedly stimulated when the mutant was grown in methanol medium.
Wang, Guanlin,Lim, Do-Seon,Choi, Baik-Dong,Park, Jin-Ju,Jeong, Soon-Jeong,Kim, Jin-Soo,Kim, Jae-Duk,Park, Jung-Su,Kim, Eung-Kwon,Kim, Byung-Hoon,Ham, Joo-Hyun,Jeong, Moon-Jin The Korean Society for Integrative Biology 2011 Animal cells and systems Vol.15 No.2
Secretory leukocyte protease inhibitor (SLPI) plays an important role in promoting the invasion and metastasis of a range of cancer cells. However, there are no reports of the expression and function of SLPI in oral carcinoma cells. In this study, the oral carcinoma cell line KB was used to determine whether SLPI affects the proliferation, migration and invasion of oral carcinoma cells. RT-PCR and Western blotting revealed high levels of endogenous SLPI expression in KB cells as well as a strong increase in SLPI secretion after wounding compared to immortalized normal oral keratinocytes (INOK). The wound healing assay revealed more migration of KB cells than INOK cells, and the SLPI treatment increased the migration of KB cells. KB cell proliferation was increased significantly by the SLPI protein but decreased by SLPI-siRNA. SLPI strongly increased the migration and invasion of KB cells. On the other hand, SLPI-siRNA decreased the migration and invasion of KB cells. This suggests that SLPI plays an important role in the metastasis of oral carcinoma cells.
Development of Two-Dimensional Scanning Videokymography for Analysis of Vocal Fold Vibration
Wang, Soo-Geun,Lee, Byung-Joo,Lee, Jin-Choon,Lim, Yun-Sung,Park, Young Min,Park, Hee-June,Roh, Jung-Hoon,Jeon, Gye-Rok,Kwon, Soon-Bok,Shin, Bum-Joo The Korean Society of Laryngology 2013 대한후두음성언어의학회지 Vol.24 No.2
Objectives : We developed two-dimensional (2D) scanning videokyomography to evaluate the mucosal wave of whole vocal cords in real time to overcome the limit of preexisting stroboscopy and line scanning videokymography which could not evaluate it. Methods : We implemented a continuous light source with high brightness, a high-definition CMOS camera, and capture board for saving the data. We created the software program to analyze the image data from the system. The test of the functionality of the 2D scanning videokymography camera was performed in one of the authors (P.H.J 32 years old male). Vocal cord images were obtained during normal phonation and falsetto phonation. Images were obtained also during cough, diplophonia. Results : The system made it possible to measure objective parameters, including fundamental frequency, amplitude, regularity, mucosal wave, and phase difference, medial and lateral peak, opening versus closing duration related to vocal fold vibration. Simultaneously, it enabled analysis of the whole mucosal wave of the entire vocal fold in real time. 2D scanning videokymography was also effective for evaluating the dynamic status of the vocal fold when the subject phonated aperiodic voice. Conclusion : In conclusion, 2D scanning videokymography can support the analysis of the whole mucosal wave of the entire vocal cord with objective vocal parameters, overcoming the limitations of stroboscopy and previous line scanning videokymography techniques.
Lim, Sungil,Park, Myoung Jun,Phuntsho, Sherub,Tijing, Leonard D.,Nisola, Grace M.,Shim, Wang-Geun,Chung, Wook-Jin,Shon, Ho Kyong Elsevier 2017 Polymer Vol.110 No.-
<P><B>Abstract</B></P> <P>A novel thin-film composite (TFC) forward osmosis (FO) membrane with dual-layered substrate membrane was fabricated by a double-blade casting technique using different polysulfone (PSf) concentrations for top (15 wt%) and bottom (7 wt%) substrate layers. Graphene oxide (GO) was incorporated in the substrate layer, and the dual casting approach resulted in a membrane support with a highly porous bottom structure and a dense top skin layer on which the polyamide active layer was effectively formed. The dual-layered TFC PSf/GO membrane (TFC-PSf<SUB>d</SUB>GO) exhibited high water permeability, and ion selectivity was enhanced by the presence of well dispersed hydrophilic GO in the PSf substrate. The TFC-PSf<SUB>d</SUB>GO also exhibited the lowest specific reverse salt flux (<I>J</I> <SUB> <I>s</I> </SUB> <I>/J</I> <SUB> <I>v</I> </SUB> = 0.19 g L<SUP>-1</SUP>) and a more favorable structural parameter (S = 130 μm) compared to GO-free membranes. Using deionized water as feed solution and 1 M NaCl as draw solution (DS), TFC-PSf<SUB>d</SUB>GO had <I>J</I> <SUB> <I>v</I> </SUB> = 33.8 L m<SUP>−2</SUP> h<SUP>−1</SUP> and <I>J</I> <SUB> <I>s</I> </SUB> = 6.9 g<SUP>−2</SUP> h<SUP>−1</SUP> under AL-FS mode, and <I>J</I> <SUB> <I>v</I> </SUB> = 61.5 L m<SUP>−2</SUP>h<SUP>−1</SUP> and <I>J</I> <SUB> <I>s</I> </SUB> = 14.0 g<SUP>−2</SUP> h<SUP>−1</SUP> under AL-DS mode. The potential of TFC-PSf<SUB>d</SUB>GO for commercial application was further evaluated by fabricating it with a fabric backing support (denoted as TFC-PSf<SUB>d</SUB>GO<SUB>f</SUB>). Compared to TFC-PSf<SUB>d</SUB>GO, TFC-PSf<SUB>d</SUB>GO<SUB>f</SUB> exhibited only 14% decline in its water flux. The overall results reveal that, fabrication of TFC substrate membrane via dual-blade casting approach along with GO incorporation produced high-performance TFC FO membranes which likely reduced the internal concentration polarization effects.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The dual-layered PSf/GO membrane was developed to mitigate the ICP effect. </LI> <LI> The dual-layered PSf substrates exhibited higher porosity and water permeability. </LI> <LI> Incorporation of GO further improved hydrophilicity of membrane substrate. </LI> <LI> The dual-layered PSf/GO membrane demonstrated the best membrane performances. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Analysis of Ploidy Levels of Korean Wild Asteraceae Species Using Chromosome Counting
Wang Yan,Jin Hee Lim,Jae A Jung,Won Hee Kim,Ki-Byung Lim,Raisa Aone M. Cabahug,Yoon-Jung Hwang 한국화훼학회 2019 화훼연구 Vol.27 No.4
Because of their attractive and colorful flowers, many species from the genus Aster serve as garden plants. Chrysanthemum owes its popularity to its ornamental and medicinal herb value. It can be used as a cut flower, potted plant, vegetable, and herbal tea. Plant breeders have attempted to identify the available species and produce new cultivars to improve the quality of chrysanthemum for commercial purposes. The use of cytogenetic studies has paved the way for identifying compatibility, ancestry, and other useful information for this undertaking. Thus, an investigation was conducted into the chromosome numbers of 23 wild Asteraceae species in Republic of Korea to determine their genetic characteristics and variations. The somatic chromosome spread has been used for chromosome counting. The results revealed that Asteraceae species have a chromosome range from 18 (diploid) to 54 (hexaploid). These findings provide primary and important information on the chromosome numbers in chrysanthemum plants that can be used to select the right variety for cultivation.
Controlled Folding of Single Crystal Graphene
Wang, Bin,Huang, Ming,Kim, Na Yeon,Cunning, Benjamin V.,Huang, Yuan,Qu, Deshun,Chen, Xianjue,Jin, Sunghwan,Biswal, Mandakini,Zhang, Xu,Lee, Sun Hwa,Lim, Hyunseob,Yoo, Won Jong,Lee, Zonghoon,Ruoff, Rod American Chemical Society 2017 Nano letters Vol.17 No.3
<P>Folded graphene in which two layers are stacked with a twist angle between them has been predicted to exhibit unique electronic, thermal, and magnetic properties. We report the folding of a single crystal monolayer graphene film grown on a Cu(111) substrate by using a tailored substrate having a hydrophobic region and a hydrophilic region. Controlled film delamination from the hydrophilic region was used to prepare macroscopic folded graphene with good uniformity on the millimeter scale. This process was used to create many folded sheets each with a defined twist angle between the two sheets. By identifying the original lattice orientation of the monolayer graphene on Cu foil, or establishing the relation between the fold angle and twist angle, this folding technique allows for the preparation of twisted bilayer graphene films with defined stacking orientations and may also be extended to create folded structures of other two-dimensional nanomaterials.</P>
Processing generates 3′ ends of RNA masking transcription termination events in prokaryotes
Wang, Xun,N, Monford Paul Abishek,Jeon, Heung Jin,Lee, Yonho,He, Jin,Adhya, Sankar,Lim, Heon M. National Academy of Sciences 2019 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.116 No.10
<P><B>Significance</B></P><P>Transcription termination by RNA polymerase in prokaryotes is well understood in contrast to similar mechanisms in higher organisms. Despite the in vitro occurrence of two types of demonstrable transcription termination events in prokaryotes at the end of transcription units, they are obscured in vivo in two ways: suppression of termination by traversing of the RNA polymerase through the termination sites when coupled to translation, or by further processing of the actual terminated RNA 3′ ends by RNases, as in eukaryotes.</P><P>Two kinds of signal-dependent transcription termination and RNA release mechanisms have been established in prokaryotes in vitro by: (<I>i</I>) binding of Rho to cytidine-rich nascent RNA [Rho-dependent termination (RDT)], and (<I>ii</I>) the formation of a hairpin structure in the nascent RNA, ending predominantly with uridine residues [Rho-independent termination (RIT)]. As shown here, the two signals act independently of each other and can be regulated (suppressed) by translation–transcription coupling in vivo. When not suppressed, both RIT- and RDT-mediated transcription termination do occur, but ribonucleolytic processing generates defined new 3′ ends in the terminated RNA molecules. The actual termination events at the end of transcription units are masked by generation of new processed 3′ RNA ends; thus the in vivo 3′ ends do not define termination sites. We predict generation of 3′ ends of mRNA by processing is a common phenomenon in prokaryotes as is the case in eukaryotes.</P>