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Cloning and Characterization of the IgA Fc Receptor from Swine
( Yumei Chen ),( Yunchao Liu ),( Gaiping Zhang ),( Hua Feng ),( Pengchao Ji ),( Guoqiang Wang ),( Chang Liu ),( Yapeng Song ),( Yunfang Su ),( Songlin Qiao ),( Aiping Wang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12
The myeloid-specific IgA Fc receptor (FcαR) is a cell surface molecule on immunocytes that provides a fundamental connection between humoral and cellular immunity. In this study, the full-length cDNA sequence of swine FcαRI (swFcαRI) was isolated and characterized and found to contain a 792-base-pair open reading frame, encoding a 264-amino-acid transmembrane glycoprotein with a predicted molecular mass of 29.4 kDa. The swFcαRI shares high amino acid sequence homology (>50%) with its counterparts from cattle, seal, and horse. Rosetting analysis confirmed that COS-7 cells transfected with an swFcαRI expression plasmid was able to combine with chicken erythrocytes sensitized with porcine IgA, but not IgG.
Chen, Chao,Wang, Yumei,Su, Chun,Zhao, Xinqing,Li, Ming,Meng, Xiaowei,Jin, Yingyu,Yang, Seung-Hwan,Ma, Yushu,Wei, Wei,Joo-Won, Suh 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.1
Passalora fulva (or Fulvia fulva) is the causal microorganism of tomato leaf mold, the outbreak of which occurs worldwide in greenhouse especially when humidity is high. However, studies on antifungal agents of P. fulva are still very limited. In this study, a marine-derived Streptomyces albidoflavus strain L131 showing potent inhibitory activities against P. fulva was identified and characterized. The active antifungal components were obtained, and studies on the antifungal mechanisms of the crude extract showed that the antifungal metabolites of L131 caused damage of hyphae and spore development, as well as plasma membrane of P. fulva. In addition, accumulation of endogenous reactive oxygen species of the leaf pathogen was also observed after treatment by culture extracts of L131. To our knowledge, this is the first report on the studies of the antifungal mechanisms against P. fulva, which benefit further development of biocontrol agent against tomato leaf mold disease.
Chen Song,Jinpeng Yu,Lin Zhao,Yumei Ma 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.2
This paper proposes a dynamic surface control-based neuroadaptive finite-time control method for induction motors (IMs) with full-state constraints and iron losses. Firstly, the barrier Lyapunov function is introduced to constrain the state variables of IMs, which ensures that the rotor angular velocity and stator current of motor are always in the given range. Secondly, the neural networks (NNs) and dynamic surface control technology are applied to approximate the unknown nonlinear functions and solve the “complexity of differentiation” problem, respectively. Then the finite-time control technology is utilized to accelerate the response speed of the system and realize the fast and effective tracking of the desired signal. At last, the effectiveness of the method is illustrated by simulation results.
Dynamic Magnetostriction Characteristics of an Fe-Based Nanocrystalline FeCuNbSiB Alloy
Lei Chen,Ping Li,Yumei Wen 한국자기학회 2011 Journal of Magnetics Vol.16 No.3
The dynamic magnetostriction characteristics of an Fe-based nanocrystalline FeCuNbSiB alloy are investigated as a function of the dc bias magnetic field. The experimental results show that the piezomagnetic coefficient of FeCuNbSiB is about 2.1 times higher than that of Terfenol-D at the low dc magnetic bias Hdc = 46 Oe. Moreover, FeCuNbSiB has a large resonant dynamic strain coefficient at quite low Hdc due to a high mechanical quality factor, which is 3-5 times greater than that of Terfenol-D at the same low Hdc. Based on such magnetostriction characteristics, we fabricate a new type of transducer with FeCuNbSiB/PZT-8/FeCuNbSiB. Its maximum resonant magnetoelectric voltage coefficient achieves ~10 V/Oe. The ME output power reaches 331.8 ㎼ at an optimum load resistance of 7 ㏀ under 0.4 Oe ac magnetic field, which is 50 times higher than that of the previous ultrasonic-horn-substrate composite transducer and it decreases the size by nearly 86%. The performance indicate that the FeCuNbSiB/PZT-8/FeCuNbSiB transducer is promising for application in highly efficient magnetoelectric energy conversion.
Chao Chen,Yumei Wang,Chun Su,Xinqing Zhao,Ming Li,Xiaowei Meng,김영우,양승환,Yushu Ma,Dong-Zhi Wei,서주원 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.1
Passalora fulva (or Fulvia fulva) is the causalmicroorganism of tomato leaf mold, the outbreak of whichoccurs worldwide in greenhouse especially when humidityis high. However, studies on antifungal agents of P. fulvaare still very limited. In this study, a marine-derivedStreptomyces albidoflavus strain L131 showing potentinhibitory activities against P. fulva was identified andcharacterized. The active antifungal components wereobtained, and studies on the antifungal mechanisms of thecrude extract showed that the antifungal metabolites ofL131 caused damage of hyphae and spore development, aswell as plasma membrane of P. fulva. In addition, accumulationof endogenous reactive oxygen species of the leafpathogen was also observed after treatment by cultureextracts of L131. To our knowledge, this is the first reporton the studies of the antifungal mechanisms againstP. fulva, which benefit further development of biocontrolagent against tomato leaf mold disease.
马玉梅 ( Ma-yumei ),陈梦瑶 ( Chen-mengyao ) 경상대학교 교육연구원 2022 중등교육연구 Vol.34 No.2
未成年人犯罪案件频发, 其犯罪的恶性程度已经远超出大众的想象, 并且已日渐成为一个世界性的难题。种种迹象表明, 未成年人犯罪日趋严重且未成年人犯罪年龄低龄化已然是一种现状, 虽然中国通过实施《刑法修正案(十一)》降低了刑事责任年龄以期进行减少犯罪和改善环境, 但这并不能从根本上解决未成年人犯罪低龄化的问题。本文通过分析未成年人犯罪的特点、低龄化趋势及成因, 认为家庭教育缺失、学校教育不足、社会风气不良、法律规制不完善等外部因素是导致低龄未成年人犯罪的主要客观原因, 未成年人自身方面心智不成熟和失衡也是犯罪的主要内因。想要从根本上预防低龄未成年人犯罪, 仅靠降低刑事责任年龄是难以达到理想治理效果的, 需要通过个人、家庭、学校、社会各界的共同推动, 同时司法方面也要积极推进, 学习借鉴各国优秀的有关未成年人犯罪的制度完善法律规制, 全面探索新时期低龄未成年人犯罪的预防和教育路径。只有依靠全社会的共同努力, 才能有效预防低龄未成年人犯罪, 保障社会秩序。 Article Abstract:Juvenile crimes occur frequently, and the malignant degree of their crimes has far exceeded the imagination of the public, and has increasingly become a worldwide problem. There are indications that juvenile delinquency is becoming more and more serious and the younger age of juvenile delinquency is already a status quo. Although China has lowered the age of criminal responsibility through the implementation of the Criminal Law Amendment (11) in order to reduce crime and improve the environment, this cannot fundamentally solve the problem of younger age of juvenile delinquency. By analyzing the characteristics, the trend and the causes of juvenile delinquency, this paper holds that the external factors, such as the lack of family education, the inadequate school education, the bad social atmosphere and the imperfect legal regulations, are the main objective causes of juvenile delinquency, and the immature and imbalance of minors' own minds are also the main internal cause of the crime. In order to fundamentally prevent juvenile delinquency, it is difficult to achieve the ideal governance effect only by lowering the age of criminal responsibility. It requires the joint efforts of individuals, families, schools and all sectors of society. And at the same time, the judiciary should actively promote, learn from the excellent systems of various countries on juvenile delinquency, improve the legal regulations, and comprehensively explore the path of prevention and education of juvenile delinquency in the new era. Only relying on the joint efforts of the whole society can we effectively prevent juvenile delinquency and ensure social order.
Involvement of Orai1 in tunicamycin-induced endothelial dysfunction
Hui Yang,Yumei Xue,Sujuan Kuang,Mengzhen Zhang,Jinghui Chen,Lin Liu,Zhixin Shan,Qiuxiong Lin,Xiaohong Li,Min Yang,Hui Zhou,Fang Rao,Chunyu Deng 대한약리학회 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.2
Endoplasmic reticulum (ER) stress is mediated by disturbance of Ca2+ homeostasis. The store-operated calcium (SOC) channel is the primary Ca2+ channel in non-excitable cells, but its participation in agent-induced ER stress is not clear. In this study, the effects of tunicamycin on Ca2+ influx in human umbilical vein endothelial cells (HUVECs) were observed with the fluorescent probe Fluo-4 AM. The effect of tunicamycin on the expression of the unfolded protein response (UPR)-related proteins BiP and CHOP was assayed by western blotting with or without inhibition of Orai1. Tunicamycin induced endothelial dysfunction by activating ER stress. Orai1 expression and the influx of extracellular Ca2+ in HUVECs were both upregulated during ER stress. The SOC channel inhibitor SKF96365 reversed tunicamycin-induced endothelial cell dysfunction by inhibiting ER stress. Regulation of tunicamycin-induced ER stress by Orai1 indicates that modification of Orai1 activity may have therapeutic value for conditions with ER stress-induced endothelial dysfunction.