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구현우,선수림,박미화,이재수,이용규,이금석,홍영식,한보광 동국대학교 전자불전연구소 2007 전자불전 Vol.9 No.-
본 연구는 한글대장경 전산화 6차 사업으로 한글대장경 30책 분량을 전산화하여 검색 시스템을 구축하는데 목적이 있다. 고려대장경의 우리말 번역본인 한글대장경을 전산화하기 위해 개역된 고문헌을 입력하여 데이터베이스로 구축하고, 인터넷을 통해 그 내용을 검색할 수 있도록 한다. 한글대장경 고문헌은 확장한자, 누락문자, 특수문자 등을 포함하고 있어서, 본 연구에서는 효과적인 입력과 저장을 위해 유니코드(Unicode)를 사용하며, 유니코드로 표현하지 못하는 문자들은 이미지 폰트를 생성하여 표현한다. 데이터베이스를 구축하기 위해서 DBMS로는 MS-SQL 7.0을 사용하고, 운영체제로는 윈도우 2000 서버를, 웹 서버로는 IIS(Internet Information Server)를 사용하여 검색 시스템을 구축하였다. 또한 다양한 검색 방법을 제공하는 검색 엔진을 개발하여, 유니코드로 저장된 한글대장경 고문헌의 내용을 웹(http://ebtc.dongguk.ac.kr/)을 통해 보다 쉽게 전 세계에서 접근할 수 있도록 한다. This research aims for constructing retrieval system by digitizing thirty books quantities of Hangul Tripitaka in the 6th Hangul Tripitaka Digitization Project. For digitizing Hangul Tripitaka which is a Korean version of the Tripitaka Korean, we can input, store the retranslated archaic documents in database, and search their contents through the Internet. Because the archaic documents of Hangul Tripitaka includes extension characters of Chinese origin, missing characters and special characters, we input and store documents use Unicode and make image fonts that cannot be represented by Unicode. Also, for developing retrieval system in this research, we use MS-SQL 7.0 and IIS(Internet Information Server) on Windows 2000 Server. By developing search engine which provides variety of retrieval methods, people who concern the archaic documents in the whole world can access Hangul Tripitaka saved as Unicode using the Web.
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PCR-Select cDNA Subtraction을 이용한 Allomyces macrogynus의 팡이실의 성장 관련 유전자의 분리
박중규,구용범 인제대학교 1998 仁濟論叢 Vol.14 No.1
Allomyces macrogyuns의 이배체 꼬리홀씨들은, 공세포로 전환된 후, 실뿌리에 이어 팡이실을 형성하는데, 팡이실로 성장하는 과정에서 대기중의 산소의 고갈로 인하여 길이 성장이 급격히 촉진된다. 본 연구에서는, 길이 성장에 관련하는 유전자를 분리하기 위해서 보통의 대기 조건에서 자란 팡이실(Normoxia)과 하나의 탈산소제를 처리한 조건에서 자란 팡이실(Hypoxia)을 수확하여 PCR을 이용한 cDNA subtraction(PVR-select cDNA subtraction)을 수행하였다. PCR로 선택된 subtracted cDNA는 pGEM-T vector에 삽입 후, 대장균에 클로닝하여, 115개의 형질전환체를 무작위적으로 골라 slot blot hybridization을 하였다. 그 결과, 대부분의 클론이 Normoxic 조건에서 보다 Hypoxic 조건에서 다량 발현되는 유전자로나타났으며, 이들 중에서 60개의 클론을 DNA 염기 서열 분석하였더니 19가지의 서로 다른 염기 서열로 동정되었다. 이 유전자들을 Genbank Database와의 유사성을 조사했을 때, 염기 서열상 이미 알려진 유전자와의 유사성이 적었으나, 아미노산 서열상으로는 몇 가지 단백질들과 유사한 것으로 나타났다. 60개의 클론 중에서 높은 빈도로 나타나는 4가지 클론을 northern hybridization하였더니, 대략적으로 각각 0.9kb, 1.9kb, 1.9kb, 2.8kb의 mRNA로 전사되는 것으로, Hypoxic 조건의 팡이실에서 특이적으로 발현되는 것으로 나타났다. Diploid zoospore of Allomyces macrogynus form hyphae after encystment and rhizoid formation. It was the linear growth of hyphae that was stimulated on their growth by oxygen depletion in the atmosphere. In this study, CDNA subtraction with PCR(PCR-select cDNA subtraction) between hyphae incubated under normal atmosphere(Normoxia) and hyphae incubated with an oxygen absorbent(Hypoxia) were performed in order to isolate the genes related to the linear growth. PCR-select Subtracted cDNAs were cloned into E. coli JM 109 using pGEM-T vector. Total 115 transformants were randomly selected and analysed by slot blot hybridization. Most of them were found to be expressed in higher level in Hypoxic condition than in Normoxic condition. Of them, 60 clones were sequenced. They were identified as 19 clones, since nucleotide sequence of 60 clones was identical. Nucleotide homology search of these clones showed no significant homology with known genes, whereas amino acid sequence homology revealed that they had some homology with known proteins. Northern hybridization analysis of 4 frequently appeared clones among 60 clones confirmed that they were expressed in higher level in Hypoxic condition and their mRNA sizes were approximately 0.9kb, 1.9kb, 1.9kb, 2.8kb, respectively.
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Aflatoxin 中毒에 의한 白鼠腺胃 粘膜의 形態的 變化에 關한 實驗的 硏究
朴龍九,梁文浩 慶熙大學校 1985 論文集 Vol.14 No.-
An experimental study for the effect of Aflatoxin B1 to the glandular stomach of the Wister rat has been carried out by autroadiography after the injection of 3H-thymidine. The Aflatoxin B1 was dissolved in dimethylsulfoxide and daily administered intraorally through catheter. 1. Hyperchromasia of the surface epithelial cell was appeared from 12 hours and getting severer after 1 week a administration. 2. Distorsion of the foveolar glands were begun to appear as early as 12 hours and getting severer after 1 week administration. 3. Focal adenomatous hyperplasia was noticed from 1 week and more evident after 6 to 8 weeks administration. 4. Nuclear atypia of the foveolar glandular cell was recognized after 1 week administration. 5. Autoradiographic labelling indices were slightly increased at the 3 days and 1 week group and then decreased at the 2 weeks and 3 weeks group. These labelling indices were persistently increased from the 4 weeks group. In conclusion, this experimental study may suggest that the Aflatoxin B1 is one of the possible substance inducing gastric carcinoma. However, further studies must be needed in order to make firm conclusion.
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디지탈 영상처리를 위한 데이타 취득 시스템에 관한 연구
박규태,진용옥,김장복,박명구,신태민,김한주 연세대학교 산업기술연구소 1983 논문집 Vol.15 No.2
A computer vision system for recognition and processing any visible objects is designed. Analog video images are digitized and stored in the internal memory of a microcomputer system and these digitized data are output through a bit-image printer. The kirsch edge operator is used for the processing. In order to compare the result of Kirsch edge enhancement, two images "a Man" and "a figure" are tested. Because of the limitations of the speed of A/D converter and the capacity of internal memory, the operation of the system is a still-picture mode. Upper 4 bit date of the memory are used for printing out the image, so as to the gray levels are sixteen. The output size of the bit-image printer is 120x120, and 2 horizontal lines are printed simultaneously per 1 carriage return because the printer has a 8 pin head. The system is designed to have compatibility with any microcomputer system which has a S-100 bus structure.
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Silicone-pyridine에 대한 Chlorophyll-a와 -b의 혼합착물의 형광연구(Ⅳ)
박면용,박택규,정구춘 建國大學校基礎科學硏究所 1987 理學論集 Vol.12 No.-
클로필-a와 -b를 혼합물의 상태로 1:2, 1:4, 1:6의 polydimethylethylsilane(0-2 -pyridylethyl methylsilane)에 Mg-pyridine 착물로 디에틸에테르중에서 결합시키고 중합체 사슬을 통한 에너지 이동을 연구하였다. Chl-b에 대한 Chl-a의 첨가량이 증가할수록 Chl-a의 혼합비(5:1, 3:1, 1:1)에 따라 형광수득율이 증가하였다. 반대로 Chl-b가 Chl-a에 비하여 첨가량이 증가할수록 형광수득율이 감소하였다. 최대 형광세기는 Chl-a와 Chl-b에 대하여 결합한 pyridine의 당량농도에서 나타났다. Chl-a에 의하여 흡수된 에너지는 중합체의 사슬을 통하여 Chl-b로 이동하였고 그 이동경향은 dimer>tetramer>hexamer의 순서로 증가하였다. The chlorophyll-a and -b have been bound 1:2, 1:4 and 1:6 polydimethyl ethyl silane(0-2-pyridylehylmethylsilane) as the mixed state to form the complex compound of Mg atom on chlorophyll and pyridine on the polymer in diethyl ether, and studied about the energy migration through the polymer chain in the same solvent. The more chl-a were added to chl-b the more increased fluorescence yields of chl-b were shown it to match for the mixed ratios of chl-a: chl-b in the order of 5:1, 3:1 and 1:1, while chl-a was shown the more decreased fluorescence yields according as the more chl-b were added. The maximum fluorescence intensity were appeared at equivalent pyridine concentration bound to the chl-a and -b. The absorbed energy by chl-a were transfered to chl-b through the chain, and the migration tendencies were on the increase of dimer>tetramer>hexamer.
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朴冕用,李文壽,鄭求春 建國大學校附設 應用科學硏究所 1978 理學論集 Vol.4 No.-
An infra-red absorption which is characteristics to the novolac resin is occurred at 1,000 ??, and this peak is useful for the identification of novolac phenol resin from resolic phenol resin. The relative intensities of infra-red absorption peaks at 813.01 and 751.88 ?? of novolac and resolic phenol resin samples did not show the significant changes for 60 min. at 140℃ and 120℃, but they were changed as a function of heat-treated time from 60 min. thereafter.
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