감시ㆍ단속적 근로자 문제점에 대한 고찰:시설경비업 중심으로

박용구,김동제,박수현 한국민간경비학회 2017 한국민간경비학회보 Vol.16 No.4

Study based on grounded theory through literature review and investigation on preceding studies and drew out problems and directions for improvement through related articles, cases and in-depth interviews with experts. Study classified drawn-out problems into 4 categories, which are salary, working hours, security work and recognition; First, intermittent security workers are afraid to be dismissed although their salary is gradually rising, which they perceive may make their employment unstable. This problem of salary basically comes from the bidding method called ‘the lowest-bidding price system.’ Second, security guards are working longer hours than other jobs, which causes various problems. Long working hours lowers work efficiency and causes health problems for the security guards and they do not have proper free time to take rest and have meals. As a measure to solve these problems, various methods of work shift can be utilized. Third, security guards are spending longer hours and making more efforts on cleaning and taking care of door-to-door packages than security works, which can lead to confusion of occupational identity and negligence of security responsibilities. Fourth, owing to the negative images of ‘employed hooligans’ or ‘servicemen’, residents are not aware of the importance of security guards and verbally abuse or even assault them and have them do what they are not supposed to do as if it were natural. Residents simply do not consider them as professionals to keep the facilities safe.

이태리포푸라 Ⅰ-214 엽육조직에서 (葉肉組織) 원형질체 분리에 미치는 몇가지 요인

박용구,손성호 ( Young Goo Park,Sung Ho Son ) 한국산림과학회 1986 한국산림과학회지 Vol.74 No.1

A method isolating Populus euramericana cv. I-214 mesophyll protoplasts was developed to facilitate application of genetic engineering techniques to this species. The suitable medium for shoot multiplication in vitro was MS basal medium with 0.1 ㎎/ℓ BAP. The effects of several factors influencing protoplast isolation could be evaluated quickly by using leaf in vitro and known volumes of maceration and washing media. The best yields of mesophyll protoplasts were obtained using leaves in vitro in 2.0% Cellulase R-10, 0.8% Macerozyme R-10, 1.2% Hemicellulase, 2.0% Driselase, 0.05% Pectolyase Y-23, and O.6M Mannitol in addition to DTT and MES buffer adjusted to pH 5.6. Over 2.4 × 10^6 protoplasts per gram of leaf were produced using these conditions. For protoplast purification, the most favorable sucrose concentration of floating solution was 0.6M after washing them with CPW solution. This method of screening factors affecting protoplast isolation could be applicable to other species.

Indonesia임업(Java를 중심으로)

박용구 한국산림경영인협회 1992 山林經營 Vol.76 No.-

아까시나무의 밀원식물 육성

박용구 경북대학교 농업과학기술연구소 한국양봉협회 한국아까시나무연구회 1993 양봉기술강습회 및 아까시나무 연구발표회 Vol.7 No.-

CT검사의 방사선 피폭과 저감화 방법

박용구,정승은 대한의사협회 2011 대한의사협회지 Vol.54 No.12

There has been a recent increase in attention focused on the potential risk of radiationinduced carcinogenesis from diagnostic radiology, with a particular emphasis on computed tomography (CT). After the rapid adoption of multidetector CT (MDCT), radiation doses from CT are now the single largest source of diagnostic radiation exposure to patients, and the carcinogenesis risk from diagnostic CT radiation dose exposure can no longer be ignored by physicians. To understand the exposure risk and monitor radiation dose exposure, an understanding and interest in CT dose reports is necessary. Almost all MDCTs now show and allow storage of the volume CT dose index (CTDIvol), dose length product (DLP), and effective dose estimations on dose reports, which are essential to assess patient radiation exposure and risks. To decrease these radiation exposure risks, the principles of justification and optimization should be followed. Justification means that the examination must be medically indicated and useful. Optimization means that the imaging should be performed using doses that are as low as reasonably achievable (ALARA), consistent with the diagnostic task. Optimization includes understanding and changing CT protocols to perform the same diagnostic task with the minimal amount of radiation exposure while maintaining diagnostic accuracy. Physicians and radiologists must be aware of the radiation risks associated with CT exams, and understand and implement the principles for patient radiation dose reduction.

잣나무의 기내배양에 관한 연구 (Ⅰ) - Callus 의 유발과 생장 -

박용구,김우룡 ( Young Goo Park,Oue Ryong Kim ) 한국산림과학회 1983 한국산림과학회지 Vol.59 No.1

Embryo tissue from Pinus koraiensis was established in culture on G.D. and W.S. basal medium supplemented with the auxin (NAA, 2,4-D, IBA) and kinetin. The various combinations of growth regulators were studied in order to determine the specific requirements of the callus tissue in vitro. The inorganic nutrient combination of G.D. medium was found to be better than that of W,S. medium. G,D. basal medium supplemented with 0.1 ppm N.AA and 0.1 ppm kinetin was the most successful nutrient combination for the growth of callus induced from the embryo of P. koraiensis.

한국에서의 지역학 논의의 재성찰 - 교토대학 동남아시아연구소의 초창기 활동에 비추어 -

박용구 한국국제지역학회 2004 한국국제지역학회보 Vol.3 No.-

포푸라 내염성(耐塩性) 개체(個體)의 기내선발(器內選拔)

박용구,손성호 박수정 경북대학교 농업과학기술연구소 1988 慶北大農學誌 Vol.6 No.-

포푸라(양황철)의 기내배양(器內培養)에서 대량증식(大量增殖)한 유전적(遺傳的)으로 균일(均一)한 시료(試料)를 이용(利用), NaCl내성(耐性)을 검정(檢定)한 결과(結果) 50mM이 생육한계(生育限界)임을 밝혀냈다. 엽표피(葉表皮)를 침(針)으로 뚫어 MS 기본배지(基本培地)에 Auxin과 Cytokinin의 효과(效果)를 검정(檢定)한 결과(結果) Cytokinin에서는 많은 개체(個體)가 발생(發生)하였으며 특(特)히 BAP 에서는 127.6개의 많은 개체(個體)가 발생(發生)하였다. NaCl 내성개체(耐性個體) 선발(選拔)을 위(爲)해 같은 배지(培地)에 NaCl 10mM부터 100mM까지 10단계로 처리(處理)하여 침(針)으로 자극한 시료(試料)를 접종(接種)한 결과(結果) 50mM 이상(以上)에서 13.7~15.7개의 여러가지 개체(個體)를 얻었다. 특(特)히 90mM에서 10.7개 100mM에서도 8.3개의 개체(個體)를 얻었다. 비선발(非選拔) 개체(個體)는 50mM 이상(以上)의 NaCl 첨가(添加)배지에서 생장(生長)이 완전(完全)히 중지(中止)된 반면(反面)에 기내선발개체(器內選拔個體)는 고농도(高濃度) NaCl의 계대배양에서도 생장(生長)를 계속하였다. 이상(以上)의 결과(結果)에서 임목(林木)에 있어서도 기내배양법(器內培養法)를 이용(利用)하여 내성(耐性) 개체(個體)를 선발(選拔)할 수 있는 가능성(可能性)을 추정(推定)할 수 있었다. The concentration of 50 mM Nacl inhibited the shoot growth of P. nigra maximowiczii in viro culture. The punctured leaves have produced so many individuals on MS basal medium with cytokinin. Especially MS basal medium with BAP 0.8mg/l showed the best shoot performance in which the average number of shoots were 127.6. For selection of NaCl tolerance shoot of poplar, punctured leaves were inoculated on MS basal medium with BAP 0.8mg/l and various concentrations of NaCl(from 10 mM to 100 mM graded by 10 mM). On the medium with over 50mM of NaCl, 13.7 to 15.7 shoots were obtained. Especially on the medium with go mM and 100mM. 10.7 and 8.3 shoots, respectively. The shoots derived from control medium (non-NaCl) were depressed growth, while the selected shoots from MS with NaCl showed good growth performance on MS basal medium with 50 mM of NaCl. From this results, we suggested that the possibility of in vitro selection to tolerance for inorganic salts in forest tree species.

부석사 조사당 선비화(禪扉花)

박용구 숲과문화연구회 2020 숲과 문화 Vol.29 No.6

수원포플러와 구아디 포플러 원형질체(原形質體) 융합(融合)에 의한 체세포잡종체(體細胞雜種體) 유도(誘導)

박용구,김정희,손성호,Park, Young Goo,Kim, Jung Hee,Son, Sung Ho 한국산림과학회 1992 한국산림과학회지 Vol.81 No.3

유망(有望) 속성수(速成樹)로 개발중(開發中)인 수원포플러(P. koreana ${\times}$ nigra var. italica)와 포플러 낙엽병(落葉病)에 내성(耐性)을 가진 구아디포플러(P. euramericana cv. Guardi)의 엽육조직(葉肉組織)에서 원형질체(原形質體)를 분리융합(分離融合)하여 잡종식물체(雜種植物體)를 생산(生?)하였다. 실험재료(實驗材料)인 수원포플러는 BA $0.5{\mu}M$, 구아디포플러는 BA $2.0{\mu}M$ 처리(處理)된 MS 배지(培地)에서 대량(大量) 증식(增殖)시킨 후 1/2 MS배지에서 계대배양(繼代培養)하여 전개(展開)된 엽조직(葉組織)을 사용(使用)하였다. 수원포플러는 효소용액(酵素溶液) I (Cellulase 2.0%, Macerozyme 0.4%, Hemicelluase 1.2%, Driselase 2.0%, Pectolyase 0.05%)에서 구아디포플러는 효소용액(酵素溶液) II (Cellulase 1.0%, Macerozyme 0.4%, Hemicellulase 1.2%, Driselase 2.0%, Pectolyase 0.05%)에서 원형질체(原形質體)를 분리(分離)하여 각각 $4.04{\times}10^7$, $2.45{\times}10^7$개의 높은 수율(收率)을 얻었다. 양수종간(兩樹種間)의 원형질체(原形質體) 융합율(融合率)은 PEG 40%가 포함(包含)된 융합용액(融合溶液)에 20분 처리(處理)하고 $Ca^{2+}$ 30mM 첨가(添加)된 희석액(稀釋液)(pH 10.5)을 사용(使用)하였을때 양수종간(兩樹種間) 1:1 융합율(融合率)이 30%정도로 높게 나타났다. 융합(融合)된 원형질체(原形質體)는 0.6M sucrose, $4.5{\mu}M$ 2, 4-D, $0.5{\mu}M$ BA가 첨가(添加)된 8p-KM에서 정치(定置) 혹은 진탕배양(震湯培養)하여 2개월후 캘루스를 얻을 수 있었으며, $5.0{\mu}M$ zeatin 처리구(處理區)에서 평균 4개의 식물체(植物體)가 재분화(再分化)되었다. 융합산물(融合?物)에서 유래(由來)한 식물체(植物體)의 잡종성(雜種性) 여부(與否)를 확인(確認)하기 위해 SDS-PAGE를 실시(實施)하였다. 모수(母樹)인 수원포플러와 구아디포플러간에는 단백질형에 차이(差異)가 있었으며 재분화(再分化) 개체중(個體中) 양친(兩親)의 중간형(中間型)을 나타내는 개체(個體)는 세포융합(細胞融合)에 의한 재분화개체(再分化個體)로 추정(推定)할 수 있었다. Protoplasts isolated from leaf mesophyll tissues of Populus koreana ${\times}$ P. nigra var. italica were fused with those of P. euramericana cv. Guardi. Well expended healthy leaves of 5 to 7 week-old-plantlet grown in vitro were used as source materials. Leaves from P. koreana ${\times}$ P. nigra var. italica and P. euramericana cv. Guardi were digested in enzyme solution I (2.0% Cellulase, 1.2% Hemicellulase, 0.4% Macrozyme, 2.0% Driselase, 0.05% Pectolyase ; w/v) and enzyme solution II (1.0% Cellulase, 1.2% Hemicellulase, 0.4% Macrozyme, 2.0% Driselase, 0.05% Pectolyase ; w/v), respectively, The highest frequency of fusion among the protoplasts originated from the two source materials was approximately 21% using 40% PEG or 15% dextran. In addition, fusion frequency was enhanced by incorporating 30mM of $Ca^{2+}$ in eluting solution at pH 10.5. Dividing cells and/or mint-calli were obtained by culturing the fusion products in a liquid 8p-KM medium supplemented with 0.6M sucrose, $0.45{\mu}M$ 2, 4-D, and $0.5{\mu}M$ BA. Shoots were regenerated from the fusion product-derived calli after culture on MS medium containing $5.0{\mu}M$ zeatin. To verify the putative hybrid or cybrid, SDS-PAGE was carried out. From the 24 regenerants, just two plants showed intermediate protein band patterns compared with those of the original source plants.