Biological Control of Apple Ring Rot on Fruit by Bacillus amyloliquefaciens 9001

Li, Yan,Han, Li-Rong,Zhang, Yuanyuan,Fu, Xuechi,Chen, Xinyi,Zhang, Lixia,Mei, Ruhong,Wang, Qi The Korean Society of Plant Pathology 2013 Plant Pathology Journal Vol.29 No.2

Apple ring rot disease, caused by Botryosphaeria dothidea (Moug. ex. Fr) Ces. et de Not., is one of the most important diseases on apple fruits. In this study, strain 9001 isolated from healthy apple fruits from an infested orchard was evaluated for its biocontrol activity against apple ring rot in vitro and in vivo. Strain 9001 showed obvious antagonistic activity to B. dothidea YL-1 when plated on potato dextrose agar. Soaking healthy apples in the bacterial suspensions of strain 9001 prior to artificial inoculation of fungal pathogen resulted in a dramatic decrease in disease incidence when compared to the control. Moreover, either field application in the growth season or postharvest treatment of apples from infected orchards with bacterial suspensions of strain 9001 resulted in significantly reduced disease incidence within the storage period for 4 months at room temperature. Based on the phylogenetic analysis of 16S rRNA and the gyrA gene, strain 9001 was identified as Bacillus amyloliquefaciens. These results indicated that B. amyloliquefaciens 9001 could be a promising agent in biocontrol of apple ring rot on fruit, which might help to minimize the yield loss of apple fruit during the long postharvest period.

Tool life evaluation of CFRP drilling with three kinds of drill

Xinyi Qiu,Pengnan Li,Changping Li,Qiulin Niu,Shujian Li,Tae Jo Ko,Lingyan Tang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.7

Serious tool wear in CFRP drilling is one of the key problems to be solved urgently. Firstly, a suitable indirect evaluation index of tool life is selected according to the literature. The critical delamination force was obtained by blind hole pushing experiment. Then, tool wear experiments were carried out with double point angle drill, stepped drill and reverse edge compound drill to analyze the variation rules of the thrust force, exit burr, exit delamination and tear with tool wear. Threshold values of exit delamination, tearing and critical thrust force were compared with each evaluation index one by one to study the causes of drills failure. The results indicated that the maximum tool wear position was the outer corner. Among the three types drills, the thrust force, delamination factor and burr angle of the reverse edge compound drill are smaller. The double point angle drill fails due to the excessive thrust force, while the stepped drill and the reverse edge compound drill fail due to the hole exit delamination exceeding the threshold. The number of drilled holes of the reverse edge compound drill is 100 % and 25 % higher than that of the double point angle drill and the stepped drill, respectively. Therefore, the reverse edge compound drill is suitable for drilling CFRP.

A Heuristic Time Sharing Policy for Backup Resources in Cloud System

( Xinyi Li ),( Yong Qi ),( Pengfei Chenand Xiaohui Zhang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.7

Cloud computing promises high performance and cost-efficiency. However, most cloud infrastructures operate at a low utilization, which greatly adheres cost effectiveness. Previous works focus on seeking efficient virtual machine (VM) consolidation strategies to increase the utilization of virtual resources in production environment, but overlook the under-utilization of backup virtual resources. We propose a heuristic time sharing policy of backup VMs derived from the restless multi-armed bandit problem. The proposed policy achieves increasing backup virtual resources utilization and providing high availability. Both the results in simulation and prototype system experiments show that the traditional 1:1 backup provision can be extended to 1:M (M≫1) between the backup VMs and the service VMs, and the utilization of backup VMs can be enhanced significantly.

Group Rejuvenation : A New Software Rejuvenation Framework

Li Su,Pengfei Chen,Yong Qi,Xinyi Li 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.6

With cloud computing continues to mature and widely used, the availability guarantee of cloud system becomes one of the key issues. In the cloud system based on virtualization, reliability of virtual machine is an important premise to guarantee the quality of service of cloud computing. Recent research shows, applications deployed inside the virtual machine will appear performance degradation even failure after long-term operation, namely "the aging phenomenon". Prediction, diagnosis and rejuvenation of the virtual machine is a relatively complete technical framework to guarantee reliability of the cloud computing system. In this paper, we propose a software rejuvenation framework based on group migration of virtual machine to guarantee reliability of the distributed system. First construct the dependency relationship of the virtual machines; then diagnosis the software aging of virtual machine; decide the optimal virtual machine set which need to migration. Compared with the traditional single computing nodes restart/ recovery mode, downtime of group rejuvenation can be 61.53% of downtime of traditional mode. In the cloud computing environment, group rejuvenation method can ensure the availability of cloud systems more effectively than the conventional rejuvenation method.

Hydrothermal Synthesis of PbTiO3 Nanocrystals with a pH-Adjusting Agent of Ammonia Solution

Xinyi Li,Zhi-Xiong Huang,Lian-Meng Zhang,Dongyun Guo 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.5

The PbTiO3nanocrystals were synthesized by a hydrothermal method, and ammonia solution was firstly used as a pH-adjustingagent. The effect of ammonia concentration on formation and morphologies of PbTiO3nanocrystals was investigated. At low ammonia concentration (0–2.2 mol/L), no perovskite PbTiO3phase was formed. When the ammonia concentrationwas 4.4 mol/L, the rod-like PbTiO3nanocrystals with highly crystalline were successfully synthesized. As the ammoniaconcentration further increased to 13.2 mol/L, the flake-like PbTiO3nanocrystals were formed.

Tannin–Thioctic Acid Network Hydrogels with Antibacterial and Hydrophobic Properties

Xinyi Hu,Que Kong,Rong Li,Change Zhou,Zhiguang Li 한국섬유공학회 2023 Fibers and polymers Vol.24 No.10

Making a multifunctional hydrogel-based wound dressing is a long-term research goal. Therefore, it is of great significance to develop an ideal wound dressing with desirable biocompatibility, adhesive property, antibacterial property and simple production. Herein, molecular network crosslinking hydrogels were achieved through the nucleophilic addition of polyphenol-sulfur radical using tannic acid and thioctic acid as matrix. The multifunctional tannic–thioctic acid (TATA) hydrogels with excellent rheological properties were prepared by further modification of antibacterial and hydrophobic properties. The successful cross-linking of the molecules was confirmed by infrared spectroscopy, Raman spectroscopy and energy dispersive X-ray spectroscopy (EDX). Complex hydrogels had certain hydrophobic properties, which can slowly absorb wound tissue fluid and keep the wetness of the wound. In addition, the hydrogel had good rheological, antibacterial and antioxidant properties, and also improved blood clotting effect and biocompatibility. These results indicated that TATA hydrogel had the potential prospect to be used as a novel wound dressing for a wide range of medical applications.

New Compound Drill Bit for Damage Reduction in Drilling CFRP

Xinyi Qiu,Pengnan Li,Changping Li,Qiulin Niu,Anhua Chen,Puren Ouyang,Tae Jo Ko 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.6 No.1

This paper focuses on finding a suitable drill bit for drilling Carbon Fiber Reinforced Plastic (CFRP). The drilling characteristics of the dagger drill, the double point angle drill, and the candle stick drill are studied. The results show that the side edge has poor ability to remove burrs, and the tip structure of the outer corner of the candle stick drill can greatly reduce the entry damage. However, the outer corner small tip structure of the candle stick drill cannot effectively remove the uncut fiber around θ = 0° of hole exit. The long secondary cutting edge of the dagger drill makes a small thrust force at the drilling exit stage and reduces the dropping speed of the thrust force, leading a lower impact on the laminate bottom. Then, a new tool is developed for drilling CFRP based on the advantages of the three kinds of drill bits. The drill has a long secondary cutting edge, a small tip diameter and a small tip structure of the outer corner. And its drilling characteristics are analyzed. The result shows that the new compound drill bit can effectively remove fibers and reduce thrust force at the drilling exit stage, form burr-free and small delamination hole. The drill reduces the waste of CFRP in manufacturing process since the exit damage is reduced substantially, which is in line with the concept of green manufacturing.

The updated role of exosomal proteins in the diagnosis, prognosis, and treatment of cancer

Wang Xinyi,Huang Jing,Chen Wenjie,Li Genpeng,Li Zhihui,Lei Jianyong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Exosomes are vesicles encompassed by a lipid bilayer that are released by various living cells. Exosomal proteins are encapsulated within the membrane or embedded on the surface. As an important type of exosome cargo, exosomal proteins can reflect the physiological status of the parent cell and play an essential role in cell–cell communication. Exosomal proteins can regulate tumor development, including tumor-related immune regulation, microenvironment reconstruction, angiogenesis, epithelial–mesenchymal transition, metastasis, etc. The features of exosomal proteins can provide insight into exosome generation, targeting, and biological function and are potential sources of markers for cancer diagnosis, prognosis, and treatment. Here, we summarize the effects of exosomal proteins on cancer biology, the latest progress in the application of exosomal proteins in cancer diagnosis and prognosis, and the potential contribution of exosomal proteins in cancer therapeutics and vaccines.

Novel Environmentally Friendly Manufacturing Method for Micro-Textured Cutting Tools

Changping Li,Xinyi Qiu,Zhen Yu,Shujian Li,Pengnan Li,Qiulin Niu,Rendi Kurniawan,고태조 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.8 No.1

Surface texturing on cutting tools has a great influence on reducing the friction force between tool and chip interfaces. In this study, a grinding method was developed to fabricate micro-groove textures on cutting tools using a special diamond grinding wheel on the tool rake face. A comparative experiment was done using a laser and other grinding method to fabricate micro-grooves. The developed grinding method does not produce harmful gases, it is environmentally friendly, and it fabricates groove textures with high efficiency and quality. Various micro-grooves with depths of 30–90 μm, groove pitches of 0.5–1 mm, and groove angles of 30–60 o were made on the rake faces of non-coated end-mill tools using the developed grinding method. Milling experiments on aluminum alloy (Al6061-T6) and tool steel (SKD11) with non-water-soluble coolant were carried out using the textured tools, and the cutting force and tool wear were investigated. In most cases, the cutting force produced by the micro-grooved tools is significantly reduced. In addition, the wear resistance of the micro-groove cutting tool is better than that of a conventional cutting tool, and there is no chipping.

Biological Control of Apple Ring Rot on Fruit by Bacillus amyloliquefaciens 9001

Yan Li,Li-Rong Han,Yuanyuan Zhang,Xuechi Fu,Ruhong Mei,Qi Wang,Lixia Zhang,Xinyi Chen 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.2

Apple ring rot disease, caused by Botryosphaeria dothidea (Moug. ex. Fr) Ces. et de Not., is one of the most important diseases on apple fruits. In this study, strain 9001 isolated from healthy apple fruits from an infested orchard was evaluated for its biocontrol activity against apple ring rot in vitro and in vivo. Strain 9001 showed obvious antagonistic activity to B. dothidea YL-1 when plated on potato dextrose agar. Soaking healthy apples in the bacterial suspensions of strain 9001 prior to artificial inoculation of fungal pathogen resulted in a dramatic decrease in disease incidence when compared to the control. Moreover, either field application in the growth season or postharvest treatment of apples from infected orchards with bacterial suspensions of strain 9001 resulted in significantly reduced disease incidence within the storage period for 4 months at room temperature. Based on the phylogenetic analysis of 16S rRNA and the gyrA gene, strain 9001 was identified as Bacillus amyloliquefaciens. These results indicated that B. amyloliquefaciens 9001 could be a promising agent in biocontrol of apple ring rot on fruit, which might help to minimize the yield loss of apple fruit during the long postharvest period.