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- 저자 : ( Taiying Li ) (검색결과 12 건)
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Taiying Li,Jin-Hyun Kim,Boknam Jung,Sungyeon Ji,Mun Won Seo,You Kyoung Han,Sung Woo Lee,Yeoung-Seuk Bae,Hong-Gyu Choi,Seung-Ho Lee,Jungkwan Lee 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.1
Background: The ascomycete fungi Cylindrocarpon destructans (Cd) and Fusarium solani (Fs) causeginseng root rot and significantly reduce the quality and yield of ginseng. Cd produces the secondarymetabolite radicicol, which targets the molecular chaperone Hsp90. Fs is resistant to radicicol, whereasother fungal genera associated with ginseng disease are sensitive to it. Radicicol resistance mechanismshave not yet been elucidated. Methods: Transcriptome analyses of Fs and Cd mycelia treated with or without radicicol were conductedusing RNA-seq. All of the differentially expressed genes (DEGs) were functionally annotated using theFusarium graminearum transcript database. In addition, deletions of two transporter genes identified byRNA-seq were created to confirm their contributions to radicicol resistance. Results: Treatmentwith radicicol resulted in upregulation of chitin synthase and cellwall integrity genes in Fsand upregulation of nicotinamide adenine dinucleotide dehydrogenase and sugar transporter genes in Cd. Genes encoding an ATP-binding cassette transporter, an aflatoxin efflux pump,ammonium permease 1 (mep1),and nitrilasewere differentially expressed in both Fs and Cd. Among these four genes, only the ABC transporterwas upregulated in both Fs and Cd. The aflatoxin efflux pump and mep1 were upregulated in Cd, but downregulatedin Fs, whereas nitrilase was downregulated in both Fs and Cd. Conclusion: The transcriptome analyses suggested radicicol resistance pathways, and deletions of thetransporter genes indicated that they contribute to radicicol resistance.
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Genetic Diversity of Epicoccum nigrum and its Effects on Fusarium graminearum
Li Taiying,Im Jihyeon,Lee Jungkwan 한국균학회 2022 Mycobiology Vol.50 No.6
Epicoccum nigrum is a saprophytic or endophytic fungus that is found worldwide. Because of the antagonist effects of E. nigrum on many plant pathogens, current studies on E. nigrum have focused on the development of biological control agents and the utilization of its vari- ous metabolites. In this study, E. nigrum was collected from a wheat field, and its genetic diversity was analyzed. Phylogenetic analyses identified 63 isolates of E. nigrum divided into seven groups, indicating a wide genetic diversity. Isolates antagonized the wheat pathogen Fusarium graminearum, and reduced disease symptoms caused by F. graminearum in wheat coleoptiles. Moreover, pretreatment of wheat coleoptiles with E. nigrum induced the upregu- lation of pathogen-related (PR) genes, PR1, PR2, PR3, PR5, PR9, and PR10 in wheat coleoptiles responding to F. graminearum invasion. Overall, this study indicates that E. nigrum isolates can be used as biological pathogen inhibitors applied in wheat fields.
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Taiying Li,Boknam Jung,Sook-Young Park,Jungkwan Lee 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.5
Survival factor 1 (Svf1) is a protein involved in cell survival pathways. In Saccharomyces cerevisiae, Svf1 is required for the diauxic growth shift and survival under stress conditions. In this study, we characterized the role of FgSvf1, the Svf1 homolog in the homothallic ascomycete fungus Fusarium graminearum. In the FgSvf1 deletion mutant, conidial germination was delayed, vegetative growth was reduced, and pathogenicity was completely abolished. Although the FgSvf1 deletion mutant produced perithecia, the normal maturation of ascospore was dismissed in deletion mutant. The FgSvf1 deletion mutant also showed reduced resistance to osmotic, fungicide, and cold stress and reduced sensitivity to oxidative stress when compared to the wild-type strain. In addition, we showed that FgSvf1 affects glycolysis, which results in the abnormal vegetative growth in the FgSvf1 deletion mutant. Further, intracellular reactive oxygen species (ROS) accumulated in the FgSvf1 deletion mutant, and this accumulated ROS might be related to the reduced sensitivity to oxidative stress and the reduced resistance to cold stress and fungicide stress. Overall, understanding the role of FgSvf1 in F. graminearum provides a new target to control F. graminearum infections in fields.
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Li, Taiying,Kim, Jin-Hyun,Jung, Boknam,Ji, Sungyeon,Seo, Mun Won,Han, You Kyoung,Lee, Sung Woo,Bae, Yeoung Seuk,Choi, Hong-Gyu,Lee, Seung-Ho,Lee, Jungkwan The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1
Background: The ascomycete fungi Cylindrocarpon destructans (Cd) and Fusarium solani (Fs) cause ginseng root rot and significantly reduce the quality and yield of ginseng. Cd produces the secondary metabolite radicicol, which targets the molecular chaperone Hsp90. Fs is resistant to radicicol, whereas other fungal genera associated with ginseng disease are sensitive to it. Radicicol resistance mechanisms have not yet been elucidated. Methods: Transcriptome analyses of Fs and Cd mycelia treated with or without radicicol were conducted using RNA-seq. All of the differentially expressed genes (DEGs) were functionally annotated using the Fusarium graminearum transcript database. In addition, deletions of two transporter genes identified by RNA-seq were created to confirm their contributions to radicicol resistance. Results: Treatment with radicicol resulted in upregulation of chitin synthase and cell wall integrity genes in Fs and upregulation of nicotinamide adenine dinucleotide dehydrogenase and sugar transporter genes in Cd. Genes encoding an ATP-binding cassette transporter, an aflatoxin efflux pump, ammonium permease 1 (mep1), and nitrilase were differentially expressed in both Fs and Cd. Among these four genes, only the ABC transporter was upregulated in both Fs and Cd. The aflatoxin efflux pump and mep1 were upregulated in Cd, but downregulated in Fs, whereas nitrilase was downregulated in both Fs and Cd. Conclusion: The transcriptome analyses suggested radicicol resistance pathways, and deletions of the transporter genes indicated that they contribute to radicicol resistance.
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Diagnosis of Cylindrocarpon destructans Using Enzyme-Linked Immunosorbent Assay
Li, Taiying,Ji, Sungyeon,Jung, Boknam,Kim, Bo Yeon,Lee, Kwang Sik,Seo, Mun Won,Lee, Sung Woo,Lee, Jungkwan,Lee, Seung-Ho The Korean Society of Plant Pathology 2019 식물병연구 Vol.25 No.3
Cylindrocarpon destructans causes ginseng root rot and produces radicicol that has an antifungal effect. In this study, we developed a method to detect this fungus using enzyme-linked immunosorbent assay (ELISA). Secreted proteins of C. destructans were used as antigens to obtain C. destructans-specific IgG from mouse. Out of 318 monoclonal antibodies generated from mouse, two antibodies (Cd7-2-2 and Cd7-2-10) showed highest specificity and sensitivity. Indirect ELISA using both antigens successfully detected C. destructans in soils, but direct ELISA using IgG conjugated with horseradish peroxidase failed to detect antigens in soils. The indirect ELISA developed here can efficiently detect the fungus and help manage ginseng root rot disease in fields.
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Li, Taiying,Jung, Boknam,Park, Sook-Young,Lee, Jungkwan The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.5
Survival factor 1 (Svf1) is a protein involved in cell survival pathways. In Saccharomyces cerevisiae, Svf1 is required for the diauxic growth shift and survival under stress conditions. In this study, we characterized the role of FgSvf1, the Svf1 homolog in the homothallic ascomycete fungus Fusarium graminearum. In the FgSvf1 deletion mutant, conidial germination was delayed, vegetative growth was reduced, and pathogenicity was completely abolished. Although the FgSvf1 deletion mutant produced perithecia, the normal maturation of ascospore was dismissed in deletion mutant. The FgSvf1 deletion mutant also showed reduced resistance to osmotic, fungicide, and cold stress and reduced sensitivity to oxidative stress when compared to the wild-type strain. In addition, we showed that FgSvf1 affects glycolysis, which results in the abnormal vegetative growth in the FgSvf1 deletion mutant. Further, intracellular reactive oxygen species (ROS) accumulated in the FgSvf1 deletion mutant, and this accumulated ROS might be related to the reduced sensitivity to oxidative stress and the reduced resistance to cold stress and fungicide stress. Overall, understanding the role of FgSvf1 in F. graminearum provides a new target to control F. graminearum infections in fields.
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Diagnosis of Cylindrocarpon destructans Using Enzyme-Linked Immunosorbent Assay
Taiying Li,Sungyeon Ji,Boknam Jung,Bo Yeon Kim,Kwang Sik Lee,Mun Won Seo,Sung Woo Lee,Jungkwan Lee,Seung-Ho Lee 한국식물병리학회 2019 식물병연구 Vol.25 No.3
Cylindrocarpon destructans causes ginseng root rot and produces radicicol that has an antifungal effect. In this study, we developed a method to detect this fungus using enzyme-linked immunosorbent assay (ELISA). Secreted proteins of C. destructans were used as antigens to obtain C. destructans-specific IgG from mouse. Out of 318 monoclonal antibodies generated from mouse, two antibodies (Cd7-2-2 and Cd7-2-10) showed highest specificity and sensitivity. Indirect ELISA using both antigens successfully detected C. destructans in soils, but direct ELISA using IgG conjugated with horseradish peroxidase failed to detect antigens in soils. The indirect ELISA developed here can efficiently detect the fungus and help manage ginseng root rot disease in fields.
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인삼내생균 Bacillus velezensis CH-15의 인삼뿌리썩음병 방제 효과
김도현,Taiying Li,이정관,이승호 한국식물병리학회 2022 식물병연구 Vol.28 No.1
Ginseng is an important medicinal plant cultivated in East Asia for thousands of years. It is typically cultivated in the same field for 4 to 6 years and is exposed to a variety of pathogens. Among them, ginseng root rot is the main reason that leads to the most severe losses. In this study, endophytic bacteria were isolated from healthy ginseng, and endophytes with antagonistic effect against ginseng root rot pathogens were screened out. Among the 17 strains, three carried antagonistic effect, and were resistant to radicicol that is a mycotoxin produced by ginseng root rot pathogens. Finally, Bacillus velezensis CH-15 was selected due to excellent antagonistic effect and radicicol resistance. When CH-15 was inoculated on ginseng root, it not only inhibited the mycelial growth of the pathogen, but also inhibited the progression of disease. CH-15 also carried biosynthetic genes for bacillomycin D, iturin A, bacilysin, and surfactin. In addition, CH-15 culture filtrate significantly inhibited the growth and conidial germination of pathogens. This study shows that endophytic bacterium CH-15 had antagonistic effect on ginseng root rot pathogens and inhibited the progression of ginseng root rot. We expected that this strain can be a microbial agent to suppress ginseng root rot.
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Mengfei Li,Huan Yang,Rongxiang Zhao,Taiying Zheng,Yi Lu,Yong Yang,Caomingzhe Si 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5
Back-to-back modular multilevel converter (B2B MMC) and modular multilevel matrix converter (M<SUP>3</SUP>C) are generally considered as two promising topologies used for soft open point (SOP) in medium-voltage distribution network (DN). SOP is utilized to control the power flowing through its grid-tied point and thus its working performance is closely related to the operating area of its topology. In this paper, considering the conditions of DN and limitations of topologies themselves, the steady-state operating areas of B2B MMC and M<SUP>3</SUP>C are calculated and analyzed in detail, which provides theoretical basis for topology selection. After establishing the equivalent circuit model, operating constraints are explored. Finally, the power regulation capacities of the two topologies in different cases are presented intuitively by P-Q graphs. The results demonstrate that B2B MMC has an advantage over M3C in operating area and it is more economical than M<SUP>3</SUP>C for transformerless SOP. The causes are also explained.
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