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Park, Jinbong,Jeon, Yong-Deok,Kim, Hye-Lin,Lim, Hara,Jung, Yunu,Youn, Dong-Hyun,Jeong, Mi-Young,Kim, Hyun-Ju,Kim, Sung-Hoon,Kim, Su-Jin,Hong, Seung-Heon,Um, Jae-Young Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-
<P>Obesity has become a major health threat in developed countries. However, current medications for obesity are limited because of their adverse effects. Interest in natural products for the treatment of obesity is thus rapidly growing. Korean Medicine (KM) is characterized by the wide use of herbal formulas. However, the combination rule of herbal formulas in KM lacks experimental evidence. According to <I>Shennong's Classic of Materia Medica</I>, the earliest book of herbal medicine, <I>Veratrum nigrum</I> (VN) has antagonistic features against <I>Panax ginseng</I> (PG), and the PG-VN pair is strictly forbidden. In this study, we have shown the effects of PG, VN, and their combination on obesity in high-fat (HF) diet-induced obese mice and in 3T3-L1 cells. PG, VN, and PG-VN combination significantly reduced weight gain and the fat pad weight in HF diet-induced obese mice. They also significantly decreased lipid accumulation and the expressions of two major adipogenesis factors, PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I>, in 3T3-L1 cells. In addition, the PG-VN combination had synergistic effects compared with the mixture of extracts of PG and VN on inhibition of PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I> expressions at lower doses. These results indicate a new potential anti-obese pharmacotherapy and also provide scientific evidence supporting the usage of herbal combinations instead of mixtures in KM.</P>
( Jung Heon Yoo ),( Jung Il Choi ),( Kyung Ock Park ),( Hye Jin Kim ),( Eun Hee Lee ),( Ha Eun Kim ),( Hyun Ju Jung ),( Sung Hoon Kwon ),( Sung Weon Ryoo ),( Yong Gyun Jung ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Due to the lack of rapid drug susceptibility test (DST) which determines the appropriate drug prescription for the TB patients, the drug resistant TB which threaten the world`s health such as multi drug-resistant (MDR) TB and extensively drug-resistant (XDR) TB are increasing. Methods: we invented a new chamber which makes a thin layer of agar mixture for rapid DST. Agarose was used for immobilizing the Mycobacterium tuberculosis (MTB) strain and 3D culture. Agarose in liquid state was mixed with MTB stock solution and the mixture was loaded in the MTB culture chamber. After solidifi cation of agarose mixture, the culture medium and drugs were supplied into the chamber. The medium and drugs were diffused into the agarose matrix and the growth of MTB was tracked by the time-lapse microscopic imaging method. The acquired images were analyzed using quantitative digital information about the growth of MTB and the DST results were provided. Results: we tested the 129 MTB strains including 27 PanS (all susceptible), 54 MDR and 48 XDR with the 4 fi rst-line TB drugs: isoniazid, rifampin, streptomycin and ethambutol. The DST results were compared with the conventional DST method with the Lowenstein-Jensen medium. There was no disagreement in 108 cases of PanS, 12 disagreements in 216 cases of MDR and 12 disagreements in 192 cases of XDR. The total disagreement rate was 4.9% in 516 cases. Conclusions: we could determine drug susceptibilities using the single cell tracking method only in fi ve days. This rapid DST method can reduce DST time dramatically and be used for fast and accurate treatments for TB patients leading the increase of the cure rate.
Oxidative modification of ferritin induced by methylglyoxal
( Sung Ho An ),( Myeong Seon Lee ),( Jung Hoon Kang ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.3
Methylglyoxal (MG) was identified as an intermediate in non-enzymatic glycation and increased levels were reported in patients with diabetes. In this study, we evaluated the effects of MG on the modification of ferritin. When ferritin was incubated with MG, covalent crosslinking of the protein increased in a time- and MG dose-dependent manner. Reactive oxygen species (ROS) scavengers, N-acetyl-L-cysteine and thiourea suppressed the MG-mediated ferritin modification. The formation of dityrosine was observed in MG-mediated ferritin aggregates and ROS scavengers inhibited the formation of dityrosine. During the reaction between ferritin and MG, the generation of ROS was increased as a function of incubation time. These results suggest that ROS may play a role in the modification of ferritin by MG. The reaction between ferritin and MG led to the release of iron ions from the protein. Ferritin exposure to MG resulted in a loss of arginine, histidine and lysine residues. It was assumed that oxidative damage to ferritin caused by MG may induce an increase in the iron content in cells, which is deleterious to cells. This mechanism, in part, may provide an explanation or the deterioration of organs under diabetic conditions. [BMB reports 2012; 45(3): 147-152]
( Jung Kyu Lee ),( Hee Jung Choi ),( Sun Mi Choi ),( Jin Woo Lee ),( Young Sik Park ),( Chang Hoon Lee ),( Jae Joon Yim ),( Chul Gyu Yoo ),( Young Whan Kim ),( Sung Koo Han ),( Sang Min Lee ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-
Background: There is limited data on serum activin-A level as a biomarker in the sepsis patients. We aimed to elucidate the diagnostic and prognostic values of serum activin-A in the management of critically ill patients with sepsis. Methods: Seventy-seven adult patients who were clinically diagnosed as sepsis and admitted in the intensive care unit (ICU) from January 2013 to March 2014, were investigated and compared with age- and sex-matched controls with tuberculosis. Serum concentration of activin-A was determined from blood sampled within 48 hours after ICU admission. Primary and secondary outcomes were the diagnostic value as biomarker of sepsis, and the prognostic value to predict the clinical outcomes of sepsis, respectively. Results: Serum activin-A level showed insignificant differences between mild sepsis and severe sepsis, but significant differences between non-sepsis and mild sepsis, and between severe sepsis and septic shock. The risk of sepsis and septic shock was significantly higher in patients with serum activin-A level more than 255 pg/mL and 432 pg/mL, respectively (aOR 50.8, 95% CI 14.8-173.8; aOR 18.8, 95% CI 2.8-124.7). Serum activin-A level had a positive correlation with SOFA score (β±SE 58.3±20.7, P=0.006), and significantly associated with ICU mortality (aOR 3.57, 95% CI 1.05-12.15). Conclusions: Serum activin-A has a diagnostic and prognostic value in critically ill patients with sepsis, and discriminant power for the sepsis severity. Serum activin-A level in early phase of sepsis is associated with SOFA score on ICU admission and ICU mortality.
Coumarins from Angelica gigas Roots having Rat Lens Aldose Reductase Activity
(Sang Hyun Lee),(Sang Hoon Jung),(Yeon Sil Lee),(Kuk Hyun Shin) 한국응용약물학회 2002 Biomolecules & Therapeutics(구 응용약물학회지) Vol.10 No.2
N/A Systematic fractionation of Angelica gigas roots led to the isolation of linear coumarins such as decursinol angelate (1), decursin (2) and nodakenin (3). These compounds were tested for their effects on rat lens aldose reductase. Nodakenin (3) was demonstrated to exhibit significant inhibition of rat lens aldose reductase activity with IC_50 value of 7.33 μM.
Jung, Jinhong,Yoon, Sang Min,Kim, So Yeon,Cho, Byungchul,Park, Jin-hong,Kim, Su Ssan,Song, Si Yeol,Lee, Sang-wook,Ahn, Seung Do,Choi, Eun Kyung,Kim, Jong Hoon BioMed Central 2013 Radiation oncology Vol.8 No.-
<P><B>Background</B></P><P>To investigate the clinical and dose–volumetric parameters that predict the risk of radiation-induced liver disease (RILD) for patients with small, unresectable hepatocellular carcinoma (HCC) treated with stereotactic body radiotherapy (SBRT).</P><P><B>Methods</B></P><P>Between March 2007 and December 2009, 92 patients with HCC treated with SBRT were reviewed for RILD within 3 months of completing treatment. RILD was evaluated according to the Common Terminology Criteria for Adverse Events, version 3.0. A dose of 10–20 Gy (median, 15 Gy) per fraction was given over 3–4 consecutive days for a total dose of 30–60 Gy (median, 45 Gy). The following clinical and dose–volumetric parameters were examined: age, gender, Child-Pugh class, presence of hepatitis B virus, gross tumor volume, normal liver volume, radiation dose, fraction size, mean dose to the normal liver, and normal liver volumes receiving from < 5 Gy to < 60 Gy (in increments of 5 Gy).</P><P><B>Results</B></P><P>Seventeen (18.5%) of the 92 patients developed grade 2 or worse RILD after SBRT (49 patients in grade 1, 11 in grade 2, and 6 in ≥ grade 3). On univariate analysis, Child-Pugh class was identified as a significant clinical parameter, while normal liver volume and normal liver volumes receiving from < 15 Gy to < 60 Gy were the significant dose–volumetric parameters. Upon multivariate analysis, only Child-Pugh class was a significant parameter for predicting grade 2 or worse RILD.</P><P><B>Conclusions</B></P><P>The Child-Pugh B cirrhosis was found to have a significantly greater susceptibility to the development of grade 2 or worse RILD after SBRT in patients with small, unresectable HCC. Additional efforts aimed at testing other models to predict the risk of RILD in a large series of HCC patients treated with SBRT are needed.</P>
Jung-Man Namgoong,Shin Hwang,Gil-Chun Park,Hyunhee Kwon,Yong Jae Kwon,Sang Hoon Kim 한국간담췌외과학회 2020 Annals of hepato-biliary-pancreatic surgery Vol.24 No.3
Orifice size of the left hepatic vein trunk (LHV) in left lateral segment (LLS) grafts is often too small to perform direct anastomosis. A small superficial branch of LHV is encountered in approximately 30% of LLS grafts. Unification venoplasty of the LHV trunk and its superficial vein branch makes the orifice size of LLS outflow vein larger than the original size. We present refined surgical techniques for LHV unification venoplasty with a superficial LHV branch. The patient was a 5-month-old 9 kg-weighing girl with biliary atresia. Her general condition deteriorated, but there was low possibility of deceased donor liver allocation, thus living donor liver transplantation was performed using her mother’s LLS. The graft hepatic vein was widened through unification venoplasty of LHV and its superficial branch. Recipient hepatic vein orifice was widened through unification of three hepatic veins. The graft and recipient hepatic vein orifices were well matched in size, and they were anastomosed with 5-0 continuous sutures. The portal vein was reconstructed with interposition of cold-preserved external iliac vein homograft. The graft left hepatic artery was reconstructed using the recipient right hepatic artery and hepaticojejunostomy was performed. This patient recovered uneventfully and is doing well for 3 months to date. The unification venoplasty with LHV trunk and its superficial vein branch makes the size of LLS outflow vein definitely larger than the original size, thus it can be a useful technical option to reduce the risk of hepatic vein outflow obstruction in pediatric liver transplantation using a LLS graft.