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Takayama, Takuya,Shin, Sohyun,Kang, SoYoung,Kim, Suk Jin,Kim, Won Seog,Ko, Young Hyeh Elsevier 2018 Human pathology Vol.73 No.-
<P>The cellular lineage of extranodal NK/T-cell lymphoma, nasal-type (ENKTL), is determined by expression of T-cell receptor (TR) or TR gene rearrangement. In ENKTL, from TR immunohistochemistry, it may often be difficult to decide whether TR-positive cells are tumor cells or not, especially when TR is expressed in a subset of tumor cells. To analyze TR expression pattern and TR rearrangement in T lineage ENKTL, we performed double immunofluorescence staining for Epstein-Barr virus encoded small RNAs (EBER)/T-cell receptor (TCR) beta F1 and CD56/TCR beta F1 in 12 cases of ENKTL that showed TCR beta F1 expression in immunohistochemistry. TR gene rearrangement was analyzed using a commercial BIOMED-2 multiplex polymerase chain reaction system. Immunohistochemistry showed that all 12 cases expressed TCR beta F1 in a wide range of infiltrating cells from 100% to <1%. Two of them expressed both TCR beta F1 and TCR c gamma M1. EBER/TCR-beta F1 positivity was confirmed in 10 cases by double staining. One case failed to show EBER/TCR-beta F1 positive cells but showed a CD56/TCR beta F1 positive result. Among 12 cases, 5 had poor-quality DNA, 3 of them showed no polymerase chain reaction product, and 2 cases showed nonspecific peak of low height. Five of 7 cases with good DNA quality demonstrated monoclonal TR gene rearrangement. Based on TR expression and TR gene rearrangement, 10 of 12 cases of ENKTL were decided as a T-lineage tumor. In conclusion, because of common TR silence and poor DNA quality, consideration of both immunohistochemistry and TR gene rearrangement is necessary to determine the lineage of ENKTL. (C) 2017 Elsevier Inc. All rights reserved.</P>
Small Angle Neutron Scattering Study on the Structural Variation of Lysozyme in Bioprotectants
Shota Koda,Haruki Takayama,Tomohiko Shibata,Tatsuya Mori,Seiji Kojima,In-Sung Park,Tae-Gyu Shin 한국물리학회 2015 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.66 No.9
The thermal denaturation and subsequent structural variation of lysozyme in various bioprotectant candidate solutions such as trehalose and choline acetate have been investigated by using small angle neutron scattering and differential scanning calorimetry. The gyration radius shows little change with the addition of additives in a native state at room temperature. On heating the lysozyme solution, a remarkable increase in the gyration radius is observed at temperatures above the denaturation temperature without any bioprotectants. Such an increase is suppressed by the additives owing to the intermolecular interactions between the lysozyme molecules and the bioprotectants of trehalose and choline acetate. The fractal dimension of lysozyme varies slightly with the addition of the bioprotectant solutions, and shows a remarkable drop in the vicinity of the denaturation temperature for all the solutions.
GABAergic transmission in developmental and degenerative hippocampus
Jin wook Kim,Yeong hoon Son,Ju hwan Kim,Su eun Lee,So hi Kang,Kyung hwan Park,Sung Ho Kim,Jong Choon Kim,Jeong tae Kim,Chitoshi Takayama,Heh In Im,Mi young Yang,Tae kyun Shin,Chang jong Moon 대한수의학회 2015 대한수의학회 학술대회발표집 Vol.2015 No.-