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Effect of Repeated Administration of Silk Peptide on the Immune System of Rats
Jae Myun Ryu,Tae Myoung Kim,Im Kwon Seo,Seongho Yeon,Woo-Taek Lim,Jeong-Yong Lee,Seock-Yeon Hwang,Namgil O,Jugyeong Song,Jinsoo Lee,Yun-Bae Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.3
In order to elucidate the possibility of influence on the immune system, silk peptide was administered to rats for 4 weeks. Male and female Sprague-Dawley rats were orally treated with low (500 ㎎/㎏) or high (2,000 ㎎/㎏) doses of silk peptide, and complete blood counts, organ weights, histopathology and cellularity of immune systems were examined. Silk peptide did not cause clinical signs, although the body weight gain at the high dose was slightly reduced. In hematological analysis, the number of white blood cells (WBC) and differential counts, the number of red blood cells (RBC) and RBC indices, and the number of platelets and mean platelet volume (MPV) were not affected by the administration of silk peptide. Especially, there were no differences in the number and rate (%) of immune cells such as lymphocytes as well as monocytes and granulocytes between vehicle and silk peptide-treated groups. Silk peptide neither affected the thymus and spleen weights, in addition to the cellularity in thymus, nor induced gross and microscopic lesions of thymus, spleen and lymph node. Taken together, 4-week administration of silk peptide up to 2,000 ㎎/㎏, the upper-limit dose in repeated-dose toxicity study, did not exhibit adverse effects on the immune systems, leading to no observed adverse effect level (NOAEL) higher than 2,000 ㎎/㎏. Without influence on the production, maturation and death process of immune cells at 20-fold dose of clinical dose (100 ㎎/㎏), silk peptide was evaluated to be a practically non-toxic material.
hnRNPK-regulated PTOV1-AS1 modulates heme oxygenase-1 expression via miR-1207-5p
( Chang Hoon Shin ),( Seongho Ryu ),( Hyeon Ho Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.4
Antisense transcripts were initially identified as transcriptional noise, but have since been reported to play an important role in the quality control of miRNA functions. In this report, we tested the hypothesis that heterogeneous nuclear ribonucleoprotein K (hnRNPK) regulates miRNA function via competitive endogenous RNAs, such as pseudogenes, long non-coding RNAs, and antisense transcripts. Based on analyses of RNA sequencing data, the knockdown of hnRNPK decreased the antisense PTOV1-AS1 transcript which harbors five binding sites for miR-1207-5p. We identified heme oxygenase-1 (HO-1) mRNA as a novel target of miR-1207-5p by western blotting and Ago2 immunoprecipitation. The knockdown of hnRNPK or PTOV1-AS1 suppressed HO-1 expression by increasing the enrichment of HO-1 mRNA in miR-1207-5p-mediated miRISC. Downregulation of HO-1 by a miR-1207-5p mimic or knockdown of hnRNPK and PTOV1- AS1 inhibited the proliferation and clonogenic ability of HeLa cells. Taken together, our results demonstrate that hnRNPKregulated PTOV1-AS1 modulates HO-1 expression via miR- 1207-5p. [BMB Reports 2017; 50(4): 220-225]
김양일(Kim, Yangil),류성호(Ryu, Seongho),신중선(Shin, Jungsun),오석환(Oh, Seokwhan) 한국신재생에너지학회 2011 한국신재생에너지학회 학술대회논문집 Vol.2011 No.05
2010년말 기준으로 약 3,200MW의 신재생에너지 발전소가 전력시장에 참여하여 전력거래를 실시하고 있다. 이 논문을 통해 2010년 1년간 운전한 신재생에너지 발전소들의 발전실적을 월별, 지역별 등으로 분석해 보고, 또한 과거 발전 실적과 비교 분석해 보고자 한다.
Chae, Ari,Jo, Seongho,Choi, Yujin,Ryu, Bowon,Choi, Cheong A.,Park, Sung Young,In, Insik Elsevier 2019 APPLIED SURFACE SCIENCE - Vol.474 No.-
<P><B>Abstract</B></P> <P>Highly water-dispersible and strongly near IR absorbing chemically reduced graphene oxide (rGO)/CP750 assembly was firstly prepared through noncovalent approach from graphene oxide (GO) and CP750, a cyclotriphosphazene ring-based tripodal amphiphile. Through the stable noncovalent interaction by tripodal CP750 amphiphiles, rGO/CP750 assembly presented both high absorption coefficient of 2260 L g<SUP>−1</SUP> m<SUP>−1</SUP> and high near infrared (NIR) absorbance (1.07 at rGO concentration of 0.05 wt.%) in aqueous media without forming precipitates for a long term storage. Upon NIR laser (808 nm) irradiation, rGO/CP750 presented high photothermal heat generating ranging from +34.1 to +78.0 °C depending on the assembly concentration. Finally, excellent photothermal bactericidal performance of nearly 100% was accomplished toward both <I>E. coli</I> and <I>S. aureus</I> even at a low concentration of assembly (0.1 mg/mL) within 10 min of NIR irradiation. In overall, our study shows that high photothermal bactericidal performance that has been mainly claimed by other strongly NIR absorbing nanomaterials such as gold nanorods and semiconducting polymers can be easily accomplished from rGO-based nanomaterials with the proper utilization of tripodal amphiphiles.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A tripodal cyclotriphosphazene ring-based tripodal amphiphile for rGO/CP750 assembly. </LI> <LI> Highly soluble and strongly NIR absorbing properties of rGO/CP750 assembly. </LI> <LI> Enhanced photothermal heat generation of rGO/CP750 assembly. </LI> <LI> Nearly 100% bactericidal performance of rGO/CP750 towards <I>E. coli</I> and <I>S. Aureus</I>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Cheol-ho Hyun,Heesoo Kim,Seongho Ryu,Won Kim 한국미생물학회 2019 The journal of microbiology Vol.57 No.11
While several methods for determining postmortem submersion interval (PMSI) in drowning cases have been suggested, the estimation of PMSI remains difficult. Next-generation sequencing (NGS) technology enables simultaneous identification of multiple taxa from environmental samples. Although NGS has been applied to estimate time since death, this application has been mainly focused on terrestrial cases. As a case study, we investigated microeukaryotic biodiversity and community structures in submerged car bonnet and drowned pig using NGS technology. NGS analysis showed that the microeukaryotic biodiversity in pig carcass was relevantly lower than that in car bonnet. NGS results also revealed that water molds and algae were related to decomposition. Relative abundances of Filobasidium, Achlya, Saprolegnia, Hydrodicton, Lobosphaera, and Scenedesmus varied with decomposition period. This data indicated that these taxa might be useful as good indicators to estimate PMSI. This study showed microeukaryotic community analysis using NGS technology may help solve drowning cases in forensic investigation.
Tae Myoung Kim,Jae Myun Ryu,Im Kwon Seo,Kyoung Mi Lee,Seongho Yeon,Shinjung Kang,Seok-Yeon Hwang,Yun-Bae Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.1
The purpose of this study was to confirm the combinational effect of red ginseng powder (RGP) and silk peptide (SP) on hypercholesterolemia and atherosclerosis in rabbits. Male New Zealand White rabbits were fed only high-cholesterol diet (HCD) containing 0.5% cholesterol and 1% corn oil for 2 weeks, and then RGP (3%), SP (1% or 3%) or their combinations were added to a diet containing 0.25% cholesterol and 1% corn oil for additional 8 weeks. Blood total cholesterol and low-density lipoproteins (LDL) were greatly increased by 2-week feeding HCD to 13-15 folds of control, in contrast to the reduction of highdensity lipoproteins (HDL) to a half level. Additional 8-week feeding HCD caused thick atheromatous plaques, covering 65.8-67.3% of the aortic wall, and hepatocytic lipid peroxidation, leading to vacuolar and fatty degeneration. Such a hypercholesterolemia was significantly reduced by addition of RGP, in comparison with a mild effect of SP. In addition, HCD-induced decrease in HDL and increase in triglycerides were reversed by RGP and SP. Moreover, the atheroma area on the aortic wall as well as hepatocytic lipid peroxidation and degeneration were markedly attenuated by RGP. Especially, the effects of RGP on blood lipid profiles, atheroma formation and hepatocytic damage were potentiated by the combination of SP. The results suggest that RGP improves hypercholestarolemia and ensuing atherosclerosis, probably by both controlling lipid metabolism and reducing free radical reactions, which is synergistically enhanced by SP.
Four-Week Repeated-Dose Toxicity of Silk Amino Acids in Rats
Tae Myoung Kim,Jae Myun Ryu,Im Kwon Seo,Seongho Yeon,Woo-Taek Lim,Jeong-Yong Lee,Seock-Yeon Hwang,Yun-Bae Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.4
This study was performed to confirm the safety of the silk amino acids (SAA). Male and female Sprague-Dawley rats were orally administered with SAA at dose levels of 500, 1,000 or 2,000 ㎎/㎏ for 4 weeks. There was no significant difference in the body weight gain between vehicle control and SAA-treated rats. Also, significant changes in daily feed and water intakes were not observed. In hematological analysis, SAA decreased the rates of monocytes and eosinophils, while increasing that of lymphocytes, suggestive of reduced inflammatory response and immune potentiation. In particular, SAA significantly increased the numbers of red blood cells, showing microcytic erythropoiesis, and platelets, implying an enhanced hematopoiesis. In blood biochemistry, alanine transaminase, aspartate transaminase and lactate dehydrogenase were significantly reduced by SAA treatment, in contrast to the increases in glucose and albumin. Blood urea nitrogen and creatine phosphokinase also significantly decreased following SAA treatment. In spite of the decreases in the markers of liver, kidneys, skeletal and heart muscles, no remarkable lesions were observed in these organs. Such effects of SAA on the blood markers might be due to the stabilization or protective activities on the active organs, which may lead to enhanced resistance to hyperactivity, hypermotility or excitatory stress. Since no observed adverse-effect level of SAA was found to be higher than the upper-limit dose (2,000 ㎎/㎏), a 20-fold higher level of a clinicallyrecommended dose (100 ㎎/㎏), it is suggested that SAA possess a long-term safety, and that it could be a candidate for the improvement of hematopoiesis and stamina.