http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Qingfeng Xie ),( Zhuo Cao ),( Weiling You ),( Xiaoping Cai ),( Mei Shen ),( Zhangyong Yin ),( Yiwei Jiang ),( Xin Wang ),( Siyu Ye ) 한국미생물생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.2
New anti-lung cancer therapies are urgently required to improve clinical outcomes. Since ganodermanontriol (GDNT) has been identified as a potential antineoplastic agent, its role in lung adenocarcinoma (LUAD) is investigated in this study. Concretely, lung cancer cells were treated with GDNT and/or mycophenolate mofetil (MMF), after which MTT assay, flow cytometry and Western blot were conducted. Following bioinformatics analysis, carboxylesterase 2 (CES2) was knocked down and rescue assays were carried out in vitro. Xenograft experiment was performed on mice, followed by drug administration, measurement of tumor growth and determination of CES2, IMPDH1 and IMPDH2 expressions. As a result, the viability of lung cancer cells was reduced by GDNT or MMF. GDNT enhanced the effects of MMF on suppressing viability, promoting apoptosis and inducing cell cycle arrest in lung cancer cells. GDNT up-regulated CES2 level, and strengthened the effects of MMF on down-regulating IMPDH1 and IMPDH2 levels in the cells. IMPDH1 and IMPDH2 were highly expressed in LUAD samples. CES2 was a potential target for GDNT. CES2 knockdown reversed the synergistic effect of GDNT and MMF against lung cancer in vitro. GDNT potentiated the role of MMF in inhibiting tumor growth and expressions of CES2 and IMPDH1/2 in lung cancer in vivo. Collectively, GDNT suppresses the progression of LUAD by activating CES2 to enhance the metabolism of MMF.
Phylogenetic Diversity of Microbial Communities in Real Drinking Water Distribution Systems
Pinpin Lu,Chao Chen,Qingfeng Wang,Zhao Wang,Xiaojian Zhang,Shuguang Xie 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.1
Microbial regrowth in drinking water distribution systems (DWDS) is a major concern in the water supply industry. Detailed knowledge of the microbial community in DWDS will be of great importance for assessing the microbiological risks of drinking water. The spatial heterogeneity of microbial community structures in the bulk waters of a large real DWDS was investigated using 16S rRNA clone library analysis. The results indicate that high residual chlorine in drinking water could not control microbial regrowth in DWDS. The bacterial communities in the bulk waters were spatially heterogenic, mainly composed of Alphaproteobacteria and Betaproteobacteria (or Cyanobacteria). Microorganisms from the genera Acinetobacter, Sphingomonas and Gemella were detected,implying there is microbiological risk from drinking water. This work provides new insight into microbial ecology in DWDS.
Identification of DNA methylation and genetic alteration simultaneously from a single blood biopsy
Chen Xiaomin,Liu Jiahui,Li Jun,Xie Yinpeng,Yu Zichen,Shen Lu,Liu Qingfeng,Wu Wei,Zhao Qiang,Lin Haoxiang,Liu Gaotong,Luo Qiuping,Yang Ling,Huang Yi,Zhao Meiru,Yi Xin,Xia Xuefeng 한국유전학회 2023 Genes & Genomics Vol.45 No.5
Background High-throughput sequencing of blood cell-free DNA (cfDNA) techniques offer an opportunity to characterize and monitor cancer rapidly in a non-invasive and real-time manner. Nonetheless, there lacks a tool within therapeutic arsenal to identify multi-omics alterations simultaneously from a single biopsy. In current times, bisulfite-based sequencing detects 5mC and 5hmC at single-base resolution is the golden standard of DNA methylation, while the degradation of DNA and biased sequencing data are the problems of this method. Objective To identify the consistency analysis of methylation and genetic variation with single library, we presented a platform detecting multi-omics data simultaneously from a single blood biopsy using bisulfite-free method of genomic methylation sequencing (GM-seq) mediated by TET enzyme. Methods We detected methylomic and genetic changes simultaneously from a single blood biopsy in NA12878 and randomly chose ten blood biopsies from colorectal cancer or lung cancer patients to validate the ability of GM-seq. Results Similar cytosine methylation level between whole genome bisulfite sequencing (WGBS) and GM-seq were identified in NA12878. Moreover, longer insert size, CpGs coverage and GC distribution were outperformed than WGBS. In addition, the comparison of the single nucleotide polymorphism (SNP), insertion-deletion (Indel) and copy number variation (CNV) in NA12878 or ctDNA from liver cancer between GM-seq and whole genome sequencing (WGS) show a good consistency, indicating that this method is feasible for detecting genetic variation in blood. Conclusion In conclusion, our work demonstrated a method for identification of the methylated modification and genetic variations simultaneously from a single blood biopsy.
DNMT1 mediates chemosensitivity by reducing methylation of miRNA-20a promoter in glioma cells
Daoyang Zhou,Yingfeng Wan,Dajiang Xie,Yirong Wang,Junhua Wei,Qingfeng Yan,Peng Lu,Lianjie Mo,Jixi Xie,Shuxu Yang,Xuchen Qi 생화학분자생물학회 2015 Experimental and molecular medicine Vol.47 No.-
Although methyltransferase has been recognized as a major element that governs the epigenetic regulation of the genome during temozolomide (TMZ) chemotherapy in glioblastoma multiforme (GBM) patients, its regulatory effect on glioblastoma chemoresistance has not been well defined. This study investigated whether DNA methyltransferase (DNMT) expression was associated with TMZ sensitivity in glioma cells and elucidated the underlying mechanism. DNMT expression was analyzed by western blotting. miR-20a promoter methylation was evaluated by methylation-specific PCR. Cell viability and apoptosis were assessed using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and TdT-mediated dUTP-biotin nick end labeling assays, respectively. The results showed that compared with parental U251 cells, DNMT1 expression was downregulated, miR-20a promoter methylation was attenuated and miR-20a levels were elevated in TMZ-resistant U251 cells. Methyltransferase inhibition by 5-aza-2′-deoxycytidine treatment reduced TMZ sensitivity in U251 cells. In U251/TM cells, DNMT1 expression was negatively correlated with miR-20a expression and positively correlated with TMZ sensitivity and leucine-rich repeats and immunoglobulin-like domains 1 expression; these effects were reversed by changes in miR-20a expression. DNMT1 overexpression induced an increase in U251/TM cell apoptosis that was inhibited by the miR-20a mimic, whereas DNMT1 silencing attenuated U251/TM cell apoptosis in a manner that was abrogated by miR-20a inhibitor treatment. Tumor growth of the U251/TM xenograft was inhibited by pcDNA-DNMT1 pretreatment and boosted by DNMT1-small hairpin RNA pretreatment. In summary, DNMT1 mediated chemosensitivity by reducing methylation of the microRNA-20a promoter in glioma cells.
Qiuling Ma,Huixuan Zhang,Yuqi Cui,Xiaoyong Deng,Ruonan Guo,Xiuwen Cheng,Mingzheng Xie,Qingfeng Cheng 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.57 No.-
Cu2O decorated TiO2 nano-tube arrays (Cu2O/TiO2 NTAs) photoelectrode was fabricated throughelectrodeposition strategy. Meanwhile, physicochemical properties of the resulting photoelectrode weresystematically studied by series of measurements. Results indicated that Cu2O/TiO2 NTAs photoelectrodeperformed a significantly enhanced light absorbance in the visible region between 400 and 800 nm,transient photocurrent density of 0.162 mA Cm 2, photogenerated charge separation efficiency and 99.9%of photoelectrocatalytic efficiency for decomposition of 2,4,6-trichlorophenol within 120 min Xenonillumination with the help of +1.0 V (vs. SCE) potential. Furthermore, the enhanced visible lightresponsible photoelectrocatalytic mechanism was proposed and discussed.
Jianfeng Gou,Xiaohui Li,Huixuan Zhang,Ruonan Guo,Xiaoyong Deng,Xiuwen Cheng,Mingzheng Xie,Qingfeng Cheng 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.59 No.-
Silver/silver chloride/exfoliated graphite (Ag/AgCl/EG) nano-photocatalyst was synthesized through precipitation and photoreduction processes. Afterwards, physicochemical properties of Ag/AgCl/EG were systematically studied by series of techniques. Results indicated that spherical-like Ag0 and AgCl with a diameter of 18.6 nm were deposited onto the surface of EG, resulting in the large surface area, strong visible absorbance and high charge separation efficiency. In addition, Ag/AgCl/EG exhibited high visible light photocatalytic (PC) activity for degradation of RhB (99.5%) and phenol (89.1%) solution. Also, the enhanced visible light PC mechanism was proposed and confirmed. Furthermore, Ag/AgCl/EG nano-photocatalyst performed good reusability even after six recycles utilization.
Yuqi Cui,Qi Meng,Xiaoyong Deng,Qiuling Ma,Huixuan Zhang,Xiaoli Li,Mingzheng Xie,Qingfeng Cheng,Xiuwen Cheng 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.43 No.-
In this study, Pt NCs/TiO2 NTAs photoelectrode was fabricated through anodization process, followed byrecycle pulse electrodeposition strategy. Subsequently, physicochemical properties of the resultingsamples were studied systematically. Results indicated that Pt NCs/TiO2 NTAs photoelectrode exhibitedintense light absorbance both in the UV and visible region, high transient photocurrent density of0.089 mA cm 2 and open circuit potential of 0.275 V cm 2. In addition, 98.3% of aspirin could beeliminated within 4 h Xenon illumination with the help of +0.4 V potential. Furthermore, it can beconcluded that two tentative pathways for PEC degradation of aspirin were proposed and confirmed.
Jianfeng Gou,Qiuling Ma,Yuqi Cui,Xiaoyong Deng,Huixuan Zhang,Xiuwen Cheng,Xiaoli Li,Mingzheng Xie,Qingfeng Cheng,Huiling Liu 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.49 No.-
In this study, high visible light response and charge separation efficiency of body-centered cubic Ag3PO4sub-microcrystals were fabricated through ultrasound-precipitation process. Additionally, the parameterswere optimized by photocatalytic (PC) removal degradation of diclofenac based on response surfacemethodology. Results indicated that the PC removal efficiency of diclofenac was significantly affected byAg3PO4 content and pH value. Under the optimized conditions, 99.9% of diclofenac could be degradedwithin 16 min Xenon illumination. Furthermore, three PC removal degradation pathways of diclofenacwere proposed, including OH-adduct to aromatic ring, direct oxidation by photoinduced holes anddecarboxylation of side chain from the parent compound.