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양막 및 탈락막 유래 중간엽 줄기세포의 분리 및 특성 규명
윤영선 ( Young Sun Yoon ),정현철 ( Hyun Chul Jeong ),황종하 ( Jong Ha Hwang ),지현준 ( Hyun Jun Jee ),석예선 ( Oye Sun Seok ),조정윤 ( Jung Youn Jo ),김윤정 ( Yun Joung Kim ),이재관 ( Jae Kwan Lee ) 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.11
Objectives: The purpose of this study is to isolate a population of multipotent cells from human amnion and decidua, respectively. Methods: Human placentas (gestational age, 30~42 weeks) were obtained after vaginal or cesarean deliveries. Amnions and deciduas were divided mechanically. The collected cells from the amnion and decidua were cultured. Cultured cells were immunophenotypically characterized. The adipogenic, osteogenic and neurogenic differentiation capacities were tested, and their growth kinetics were analyzed. Results: We successfully isolated MSCs from both the amnion and decidua. The phenotype of MSCs cultured from different fetal and maternal parts of the placenta was comparable. The growth kinetics of MSCs derived from amnions and deciduas were similar. Isolated MSCs were differentiated into various cell lines such as adipogenic, osteogenic, myogenic and neurogenic cells. Conclusions: The human amnion and decidua could be an excellent source of MSC because they are easily obtainable after delivery and showed a higher expansion capacity than that of MSCs from adult bone marrow.
Hwang, Jong Ha,Seok, Oye Sun,Song, Hae-Ryong,Jo, Jung Youn,Lee, Jae Kwan Mary Ann Liebert, Inc 2009 Cloning & stem cells Vol.11 No.2
<P>The HOX family of genes plays a fundamental role in the morphogenesis of vertebrate embryonic cells. HOX genes are thought to be important for the regulation of stem cells. We investigated HOX gene expression in mesenchymal stem cells (MSCs) from human placentas. We isolated MSCs from human placentas and confirmed stemness by fluorescence-activated cell sorting (FACS) analysis and differentiation studies. Using reverse transcription PCR, mRNA expression of 39 Class I HOX genes was measured in the MSCs. The expression of HOXB6, C4, C8, C10, D3, D4, and D10 were measured by Western blot analysis. HOXC10 was expressed in 10 of 10 amnion-derived MSCs but in only 2 of 10 decidua-derived MSCs. HOXC4 and D10 were expressed in 100% of both amnion-derived MSCs and deciduas-derived MSCs. HOXD4 was silent in all amnion-derived MSCs and deciduas-derived MSCs (n = 10). HOX gene activation patterns might be a useful indicator for the detection of MSCs of different tissue origins. We demonstrated that HOXC10 is a gene that may discriminate between amnion-derived MSCs and decidua-derived MSCs.</P>
The altered vascular reactivity in offspring from pregnant mice model of preeclampsia
( Geum Joon Cho ),( Hye Mi Jin ),( Oye Sun Seok ),( Min Woo Kim ),( Suhng Wook Kim ),( Suk Hyo Suh ),( Soon Cheol Hong ),( Hai Joong Kim ),( Min Jeong Oh ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: The aim of this was to evaluate the effect of maternal preeclampsia on vascular reactivity of offspring in a pregnant mouse model of preelampsia. 방법: At day 8 of gestation pregnant CD-1 mice were randomly allocated to injection using the tail vein of the lentivirus carring sFlt-1 (109 plaque- forming units in 100 microliters; sFlt-1 group) or the mock lentivirus (109 plaque-forming units in 100 microliters; mock group used as a control for the virus) or control group. After delivery, nursing dams and offspring were kept together in individual cages. Offspring from each group were sacrificed at 8 weeks of age, and abdominal aorta was harvested for the study of vascular reactivity. 결과: The vasorelaxant responses to acetylcholine (endothelium-dependent relaxant) were decreased in offspring from sFlt-1 group compared with offspring from control and mock groups. However, there was no difference in vasorelaxant responses to sodium nitroprusside (the endothelium- independent relaxant) between four groups. 결론: Maternal preeclampsia has detrimental effects on vascular reactivity in offspring. Our findings show hypertension of fetal programming in offspring born to preeclamptic mothers.
Hwang, Jong Ha,Shim, Soung Shin,Seok, Oye Sun,Lee, Hang Young,Woo, Sang Kyu,Kim, Bong Hui,Song, Hae Ryong,Lee, Jae Kwan,Park, Yong Kyun The Korean Academy of Medical Sciences 2009 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.24 No.4
<P>Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation into lineages of mesenchymal tissues that are currently under investigation for a variety of therapeutic applications. The purpose of this study was to compare cytokine gene expression in MSCs from human placenta, cord blood (CB) and bone marrow (BM). The cytokine expression profiles of MSCs from BM, CB and placenta (amnion, decidua) were compared by proteome profiler array analysis. The cytokines that were expressed differently, in each type of MSC, were analyzed by real-time PCR. We evaluated 36 cytokines. Most types of MSCs had a common expression pattern including MIF (GIF, DER6), IL-8 (CXCL8), Serpin E1 (PAI-1), GROα(CXCL1), and IL-6. MCP-1, however, was expressed in both the MSCs from the BM and the amnion. sICAM-1 was expressed in both the amnion and decidua MSCs. SDF-1 was expressed only in the BM MSCs. Real-time PCR demonstrated the expression of the cytokines in each of the MSCs. The MSCs from bone marrow, placenta (amnion and decidua) and cord blood expressed the cytokines differently. These results suggest that cytokine induction and signal transduction are different in MSCs from different tissues.</P>
The effect of pravastatin on vascular reactivity in pregnant mice model of preeclampsia
( Geum Joon Cho ),( Ji Hye Heo ),( Min Woo Lee ),( Suhng Wook Kim ),( Oye Sun Seok ),( Suk Hyo Suh ),( Soon Cheol Hong ),( Hai Joong Kim ),( Min Jeong Oh ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-
The aim of this was to evaluate the effect of pravastatin on vascular reactivity in a pregnant mouse model of preelampsia. At day 8 of gestation pregnant CD-1 mice were randomly allocated to injection using the tail vein of the lentivirus carring sFlt-1 (109 plaque- forming units in 100 microliters; sFlt-1 group) or the mock lentivirus (109 plaque-forming units in 100 microliters; mock group used as a control for the virus) or control group and then to receive pravastatin (5 mg/kg/d) dissolved in drinking water (sFlt-1-pravastatin group). The mice in four groups (sFlt-1, sFlt-1-pravastatin, mock and control) were killed at day 18 of gestation and abdominal aorta was harvested for the study of vascular reactivity. The vasorelaxant responses to acetylcholine (endothelium-dependent relaxant) were decreased in sFlt-1 group compared with control and mock groups. However, the vasorelaxant responses were increased in sFlt-1-pravastatin group compared with sFlt-1 group. There was no difference in vasorelaxant responses to sodium nitroprusside (the endothelium-independent relaxant) between four groups. In this study, pravastatin improved the detrimental effect of over-expression of sFlt-1 on vascular reactivity in a pregnant mouse model of preeclampsia. Pravastatin may provide potential therapeutic approach for the prevention and treatment of preeclampsia.a