Roles of Immunohistochemical Staining in Diagnosing Pulmonary Squamous Cell Carcinoma

Yan, Yue,Zhang, Ya-Xiong,Fang, Wen-Feng,Kang, Shi-Yang,Zhan, Jian-Hua,Chen, Nan,Hong, Shao-Dong,Liang, Wen-Hua,Tang, Yan-Na,He, Da-Cheng,Wu, Xuan,Zhang, Li Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Background: Differentiating morphologic features based on hematoxylin-eosin (HE) staining is the most common method to classify pathological subtypes of non-small-cell lung cancer (NSCLC). However, its accuracy and inter-observer reproducibility in pathological diagnosis of poorly differentiated NSCLC remained to be improved. Materials and Methods: We attempted to explore the role of immunohistochemistry (IHC) staining in diagnosing pulmonary squamous cell carcinoma (SQCC) with poorly differentiated features by HE staining or with elevated serum adenocarcinoma-specific tumor markers (AD-TMs). We also compared the difference of epidermal growth factor receptor (EGFR) mutation rate between patients with confirmed SQCC and those with revised pathological subtype. Logistic regression analyses were used to test the association between different factors and diagnostic accuracy. Results: A total of 132 patients who met the eligible criteria and had adequate specimens for IHC confirmation were included. Pathological revised cases in poor differentiated subgroup, biopsy samples and high-level AD-TMs cases were more than those with high/moderate differentiation, surgical specimens and normal-level AD-TMs. Moreover, biopsy sample was a significant factor decreasing diagnostic accuracy of pathological subtype (OR, 4.037; 95% CI 1.446-11.267, p=0.008). Additionally, EGFR mutation rate was higher in patients with pathological diagnostic changes than those with confirmed SQCC (16.7% vs 4.4%, p=0.157). Conclusions: Diagnosis based on HE staining only might cause pathological misinterpretation in NSCLC patients with poor differentiation or high-level AD-TMs, especially those with biopsy samples. HE staining and IHC should be combined as pathological diagnostic standard. The occurrence of EGFR mutations in pulmonary SQCC might be overestimated.

CMTM5-v1, a four-transmembrane protein, presents a secreted form released via a vesicle-mediated secretory pathway

( He Nan Li ),( Xiao Huan Guo ),( Lu Ning Shao ),( Markus Plate ),( Xiao Ning Mo ),( Yu Wang ),( Wen Ling Han ) 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.3

The CKLF-like MARVEL transmembrane domain-containing family (CMTM) is a novel family of proteins linking classical chemokines and the transmembrane 4 superfamily (TM4SF). Our earlier studies indicated several CMTM members (such as CKLF1 and CMTM2) have a secreted form. This is the first report of the secreted form of CMTM5-v1, the major RNA splicing form of CMTM5, which is produced as small vesicles (<100 nm diameter) and floats at a peak density of 1.19 g/ml on continuous sucrose gradients. CMTM5-v1 has no obvious co-localization with CD63 or Golgi complex. In addition, brefeldin A but not wortmannin can inhibit the secretion of CMTM5-v1. Our results suggest that CMTM5-v1 might be secreted via a different vesicle-mediated secretory pathway, which will be helpful for the studies of vesicle-mediated secretion and MARVEL domain-containing proteins. [BMB reports 2010; 43(3): 182-187]

2-Hydroxy-3-methylanthraquinone from Hedyotis diffusa Willd induces apoptosis in human leukemic U937 cells through modulation of MAPK pathways

Nan Wang,Dong-Yang Li,Hui-Yan Niu,Yi Zhang,Ping He,Jia-He Wang 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.6

The herb of Hedyotis diffusa Willd (H. diffusaWilld), an annual herb distributed in northeastern Asia, hasbeen known as a traditional orientalmedicine for the treatmentof cancer. Recently, Chinese researchers have discovered thattwo anthraquinones isolated from a water extract of H. diffusaWilld showed apoptosis-inducing effects against cancer cells. However, the cellular and molecular mechanisms responsiblefor this phenomenon are poorly understood. The current studydetermines the role of mitogen-activated protein kinases(MAPK) in human leukemic U937 cells apoptosis inducedby 2-hydroxy-3-methylanthraquinone from H. diffusa. Ourresults showed that 2-hydroxy-3-methylanthraquinone decreasedphosphorylation-ERK1/2 (p-ERK1/2), and increasedp-p38MAPK, but did not affect expressions of p-JNK1/2 inU937 cells. Moreover, treatment of U937 cells with2-hydroxy-3-methylanthraquinone resulted in activation ofcaspase-3. Furthermore, PD98059 (ERK1/2 inhibitor)significantly enhanced 2-hydroxy-3-methylanthraquinoneinducedapoptosis in U937 cells, whereas caspase-3 inhibitoror SB203580 (p-p38MAPK inhibitor), decreased apoptosis inU937 cells. Taken together, our study for the first time suggeststhat 2-hydroxy-3-methylanthraquinone is able toenhance apoptosis of U937 cells, at least in part, throughactivation of p-p38MAPK and downregulation of p-ERK1/2. Moreover, the triggering of caspase-3 activation mediatedapoptotic induction.

Focus on Traditional Clothing Culture

He Nan Li 한국콘텐츠학회 2012 ICCC International Digital Design Invitation Exhib Vol.8 No.1

Routine Screening and Consultation Facilitate Improvement of Metabolic Syndrome

Yoon, Nan-He,Yoo, Seunghyun,Kim, Hyekyeong,Han, Yoonjung The Korean Academy of Medical Sciences 2015 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.30 No.8

Competing Narratives, Identity Politics, And Cross-Strait Reconciliation

( Yi Nan He ) 경남대학교 극동문제연구소 2010 ASIAN PERSPECTIVE Vol.34 No.4

After nearly sixty years of political confrontation, hopes for cross-Taiwan Strait reconciliation have run high since the traditionally pro-unification Nationalist Party (the Kuomintang, KMT) returned to power in Taiwan in May 2008. However, obstacles to reconciliation remain daunting, due to a fundamental disjuncture between the ideological beliefs of the two sides, in particular because China and Taiwan still lack a shared memory of Taiwanese history that can serve as the foundation for their reconciliation. This article examines a wide variety of sources from Taiwan and China over recent decades. It illustrates their conspicuous memory gap over the history of the island. Cross-Strait reconciliation needs to begin with recognizing rather than ignoring or covering up the memory gap. Dialogue and joint studies should be carried out to better understand each other`s political perspective and emotional appeal associated with historical memory.

Solving UTP Containing Combining Classes using GA

Zhang He-nan,ZhangShao-wen 보안공학연구지원센터(IJUNESST) 2014 International Journal of u- and e- Service, Scienc Vol.7 No.4

생애말기 노인의 장기요양서비스 이용 특성과 영향요인

윤난희 ( Nan-he Yoon ),김홍수 ( Hongsoo Kim ),권순만 ( Soonman Kwon ) 한국보건행정학회 2016 보건행정학회지 Vol.26 No.4

Background: The purpose of this study is to examine the characteristics of and factors associated with long-term care (LTC) utilization under public long-term care insurance (LTCI) among end-of-life older adults in Korea. Methods: Using a 5% sample of older people aged 65 or older and their health and LTC insurance data, two-part model analyses were conducted. We compared LTC uses and their determinants during the last year of life among decedents in the year 2010 with those of survivors. We also compared the medical uses of the same sample with their LTC uses. Results: The end-of-life elderly were more likely to use LTC, and their expenditure on LTC was higher than their counterparts. Whether or not older people used LTC during their last year of life was significantly affected by age, sex, health insurance, household income, and living alone; however, LTC costs of the decedents were only affected by functional status, which may have been due to the reimbursement scheme of the current LTCI, which is mainly based on functional dependency level. For the survivors, having chronic diseases significantly increased the likelihood of LTC use, which was not the case for the decedents. End-of-life elderly with relatively low social economic status were more likely to use the LTC other than medical services, while the health conditions affected their medical uses most significantly. Conclusion: The study findings provide key information for predicting demand related to the increasing LTC needs of Korean older people at the end of life.

아동의 비만과 부모의 영향요인

윤난희 ( Nan-he Yoon ) 대한보건협회 2018 대한보건연구 Vol.44 No.3

연구목적: 본 연구는 아동의 비만과 그에 영향을 미치는 부모의 요인을 확인하고, 부모의 비만 여부에 따른 아동의 비만 발생 추정 확률을 비교하고자 수행되었다. 연구방법: 본 연구는 제6기(2013-2015년) 국민건강영양조사 자료를 활용하였으며, 조사 응답 가구 중 부모 모두의 정보가 수집된 만 3세 이상 13세 미만 아동 1,064명을 대상으로 분석을 실시하였다. 아동의 성별과 연령, 거주지역, 가구소득, 부모의 교육수준 등의 일반적 특성과 부모의 식생활 및 신체활동 실천 요인들에 따른 비만율의 분포를 확인하고 카이제곱 검정을 통해 비교하였다. 그리고 로지스틱회귀분석을 활용하여 이러한 잠재적 영향요인들을 통제한 후 부모의 비만이 아동의 비만에 미치는 영향을 확인하였으며, 학령기와 학령전기 아동을 구분하여 층화분석을 실시하였다. 로지스틱회귀분석 결과를 통해 아동의 비만 발생 추정 확률을 산출하였고, 부모의 비만 여부에 따른 차이를 일원배치분산분석을 통해 확인하고 Tukey 사후검정 방법을 적용하여 비교하였다. 연구결과: 전체 연구대상자 1,064명 중 64명의 아동(6.0%)이 비만이었다. 이들의 인구사회학적 특성과 부모의 건강행태 요인 등을 통제한 로지스틱회귀분석 결과, 부모가 비만인 아동에게서 비만이 유의하게 많은 것으로 확인되었으며, 층화분석 결과에 따르면 특히 학령전기 아동에게서 이러한 경향이 더 강하게 나타났다. 한편 아동과 부모의 잠재적 영향요인들을 보정하여 산출한 아동의 비만 발생 추정 확률을 살펴보면, 부모가 모두 비만인 경우에 아동이 비만일 확률이 가장 높았다. 이러한 경향은 학령기와 학령전기를 구분해도 동일하게 나타났으나 어머니가 비만인 학령기 아동의 비만 발생 추정 확률은 아버지가 비만인 경우에 비해 유의하게 높은 것으로 확인되었다. 결론: 전 세계적으로 급증하는 아동 비만을 효과적으로 예방하고 관리하기 위하여 부모의 비만 여부에 따라 아동 비만 관리의 고위험군 대상 집단을 선정하고 부모와 가족들이 함께 참여하는 전략의 마련이 필요하다. Objective: This study aimed to identify the influences of parental factors on childhood obesity in Korea. Methods: Data from 2013-2015 Korea National Health and Nutrition Examination Survey were used for the analysis. Multivariate logistic regression analysis was conducted to analyze effects of the parental factors on childhood obesity. Stratified analysis was also conducted to compare the effects between preschool-aged and school-aged children. Predicted probabilities of childhood obesity were calculated after adjusting for covariates including sociodemographic characteristics, parents’ dietary behaviors, and physical activity. The probabilities were compared according to the obesity of parents using one-way ANOVA and Tukey's honestly significant difference (HSD) post-hoc test. Results: Of the 1,064 respondents, 64 (6.0%) were obese. After controlling for their sociodemographic factors and parents’ health behaviors, the children with obese parents were more likely to be obese than their counterparts. According to the stratified analysis, the effects of parental factors on childhood obesity were more significant among preschool-aged children. The predicted probability of obesity was highest among children whose both parents were obese, and the predicted probability among school-aged children whose mothers were obese were higher than those whose fathers were obese. Conclusion: To prevent and manage childhood obesity effectively, it is necessary to involve their parents and family targeting high risk group from those whose both parents’ are obese.

Involvement of Phosphatidylinositol 3-Kinase/Akt Signaling Pathway in β1 Integrin-Mediated Internalization of Staphylococcus aureus by Alveolar Epithelial Cells

Jia-He Wang,Ke Zhang,Nan Wang,Xiao-Min Qiu,Yi-Bing Wang,Ping He 한국미생물학회 2013 The journal of microbiology Vol.51 No.5

The invasion of Staphylococcus aureus into alveolar epithelial cells is regarded as the key step for S. aureus lung infection. However, the mechanism of internalization of S. aureus by alveolar epithelial cells is not clear, and was the aim of this investigation Human lung adenocarcinomic epithelial cells and A549 cells were used. Human β1 integrin and rat β1 integrin were detected by real-time reverse transcription (RT)-PCR. The expressions of β1 integrin, Akt and p-Akt were detected by Western blot analysis. To further investigate the role of β1 integrin in S. aureus internalization by alveolar epithelial cells, we next performed siRNA-mediated knockdown of β1 integrin expression. In this study, we found that S. aureus invades human alveolar epithelial cells and rat primary alveolar epithelial cells. The β1 integrin ligand competitive inhibitor, GRGDS-peptide, blocked the internalization of S. aureus by A549 cells. Knockdown of β1integrin also inhibited the internalization of S. aureus. In addition, the PI3K/Akt signaling pathway in alveolar epithelial cells was activated by the infection with S. aureus. Furthermore, Akt phosphorylation was abolished by transient transfection with β1 integrin siRNA in A549 cells challenged with S. aureus. Our results suggest that the phosphatidylinositol 3-kinase/Akt signaling pathway plays an important role in β1 integrin-mediated internalization of S. aureus by alveolar epithelial cells.