CdSe@ZnS/ZnS quantum dots loaded in polymeric micelles as a pH-triggerable targeting fluorescence imaging probe for detecting cerebral ischemic area

Yang, Hong Yu,Fu, Yan,Jang, Moon-Sun,Li, Yi,Yin, Wen Ping,Ahn, Tae Kyu,Lee, Jung Hee,Chae, Heeyeop,Lee, Doo Sung Elsevier 2017 Colloids and surfaces. B, Biointerfaces Vol.155 No.-

<P><B>Abstract</B></P> <P>High photoluminescence (PL) quantum yield (QY), photostability CdSe@ZnS/ZnS core/multishell quantum dots (CM-QDs) were first applied for bioimaging. The solubility, stability and biocompatible of the fluorescence imaging probes were constructed by self-assembly of CM-QDs and pH-responsive methoxy poly(ethylene glycol)-poly(β-amino ester/amidoamine)-dodecylamine (mPEG-PAEA-DDA) multiblock copolymers. The resulting CM-QDs-loaded mPEG-PAEA-DDA micelles (CM-QDs-PEG-PAEA-DDA) exhibited lower cell cytotoxicity and higher fluorescence intensity than the core/shell CdSe@ZnS QDs-encapsulated mPEG-PAEA-DDA micelles (CS-QDs-PEG-PAEA-DDA). Moreover, the in vivo fluorescence imaging ability confirmed that the CM-QDs-PEG-PAEA-DDA can be employed as a pH-triggerable targeting imaging probe for detection of a rat bearing cerebral ischemia disease. Therefore, we believed that the CM-QDs-PEG-PAEA-DDA may be the next generation of fluorescence imaging probes for targeted diagnosis acidic pathological areas, using pH as a stimulus.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CdSe@ZnS/ZnS QDs are synthesized and first applied for bioimaging. </LI> <LI> mPEG-PAEA-DDA copolymer showed pH-responsive property and the hydrolysis stability. </LI> <LI> CM-QDs-PEG-PAEA-DDA exhibited lower toxicity and higher fluorescent intensity. </LI> <LI> CM-QDs-PEG-PAEA-DDA has ability to target image for acidic brain ischemic area. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Expression of Endogenous Hypoxia Markers in Vulvar Squamous Cell Carcinoma

Li, Yu-Zhu,Li, Shu-Ling,Li, Xia,Wang, Li-Jie,Wang, Jiu-Ling,Xu, Jia-Wen,Wu, Zhi-Hong,Gong, Li,Zhang, Xiao-Dan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Objective: To investigate the expression of endogenous hypoxia-related markers identified as being involved in vulvar squamous cell carcinoma (VSCC). Methods: We performed immunohistochemical staining of hypoxia-inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$), glucose transporter-1 (GLUT-1), carbonic anhydrase 9 (CA-9) and vascular endothelial growth factor (VEGF), on tissue sections of 25 VSCC patients, 10 vulvar intraepithelial neoplasia (VIN) patients and 12 healthy controls. Results: HIF-$1{\alpha}$ expression was found in all sections, with no significant difference between controls, VIN and VSCC sections (all P<0.05). Glut-1 expression was found in 25% of control, 90% of VIN and 100% of VSCC sections. A significant difference between control and VIN or VSCC was observed (all P<0.05), while no difference was found between VIN and VSCC sections (P>0.05). CA-9 expression was negative in control sections, but it was found in 30% of VIN sections and 52% of VSCC sections with strong staining. Similarly, CA-9 expression also showed obvious differences between controls and VIN or VSCC sections (all P<0.05). However, there was no significant difference between VIN and VSCC (P>0.05). There were only 25% of control sections with weak VEGF expression, while strong staining was found in about 60% of VIN sections and 25% of VSCC sections (all P<0.05). In addition, a difference was also found between VIN and VSCC sections (P<0.05). Conclusion: Expression of endogenous hypoxia markers (HIF-$1{\alpha}$, GLUT-1, CA-9 and VEGF) might be involved in the malignant progression of VSCC.

T-SPOT.TB for Detection of Tuberculosis Infection among Hematological Malignancy Patients and Hematopoietic Stem Cell Transplant Recipients

Qin, Li-Li,Wang, Qin-Rong,Wang, Qian,Yao, Hong,Wen, Li-Jun,Wu, Li-Li,Ping, Na-Na,Xie, Jun-Dan,Chen, Mei-Yu,Chen, Su-Ning Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

The diagnosis of latent Mycobacterium tuberculosis infection (LTBI) is recommended in hematological malignancy patients and before hematopoietic stem cell transplantation (Guidelines for the prevention and management of infectious complications of solid organ transplantation, 2004). Compared to traditional methods such as tuberculin skin test (TST), T-SPOT.TB has been shown to be more specific. In the present study we enrolled 536 patients for whom T-SPOT.TB was performed, among which 295 patients also received the TST test. The agreement (79%) between T-SPOT.TB and TST was poor (x=0.274, P<0.001). The patients with positive T-SPOT.TB results numbered 62 (11.6%), in which only 20 (48.8%) of the 41 receiving the TST test had positive results. A majority of the patients with T-SPOT.TB positive results had some other evidence ofTB, such as TB history, clinical symptoms and an abnormal chest CT scan. Active TB was found in 9 patients, in which 2 had negative TST results. We followed up the patients and no one developed active TB. Our study suggested that the T-SPOT.TB may be more useful for screening LTBI and active TB in hematological malignancy patients and hematopoietic stem cell transplant recipients than the TST test.

BRCA1 Gene Exon 11 Mutations in Uighur and Han Women with Early-onset Sporadic Breast Cancer in the Northwest Region of China

Cao, Yu-Wen,Fu, Xin-Ge,Wan, Guo-Xing,Yu, Shi-Ying,Cui, Xiao-Bin,Li, Li,Jiang, Jin-Fang,Zheng, Yu-Qin,Zhang, Wen-Jie,Li, Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

The prevalence of BRCA1 gene mutations in breast cancer differs between diverse ethnic groups. Relatively little information is known about patterns of BRCA1 mutations in early-onset breast cancer in women of Uighur or Han descent, the major ethnic populations of the Xinjiang region in China. The aim of this study was to identify BRCA1 mutations in Uighur and Han patients with early-onset (age <35 years), and sporadic breast cancer for genetic predisposition to breast cancer. For detection of BRCA1 mutations, we used a polymerase chain reaction single-stranded conformation polymorphism approach, followed by direct DNA sequencing in 22 Uighur and 13 Han women with early-onset sporadic breast cancer, and 32 women with benign breast diseases. The prevalence of BRCA1 mutations in this population was 22.9% (8/35) among early-onset sporadic breast cancer cases. Of these, 31.8% (7/22) of Uighur patients and 7.69% (1/13) of Han patients were found to have BRCA1 mutations. In 7 Uighur patients with BRCA1 mutations, there were 11 unique sequence alterations in the BRCA1 gene, including 4 clearly disease-associated mutations on exon 11 and 3 variants of uncertain clinical significance on exon 11, meanwhile 4 neutral variants on intron 20 or 2. None of the 11 BRCA1 mutations identified have been previously reported in the Breast Cancer Information Core database. These findings reflect the prevalence of BRCA1 mutations in Uighur women with early-onset and sporadic breast cancer, which will allow for provision of appropriate genetic counseling and treatment for Uighur patients in the Xinjiang region.

High-accuracy electrohydraulic control system for the position and orientation of the primary mirror for a large telescope

Li Yu-Xia,Wang Jian-Li,Guo Peng-Fei,Li Hong-Wen,Cao Yu-Yan 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.79 No.7

Due to variations in gravity, temperature, and external disturbances, the optical axes of a telescope’s primary mirrors can shift and, consequently, become misaligned with the light path. Such misalignment significantly reduces image quality; thus, the position and orientation of primary mirror (POPM) control systems must maintain the optical axis in an ideal position. Therefore, in this paper, we proposes a novel high-accuracy electrohydraulic control system for the position and orientation of the primary mirror (POPM) of a large telescope. To this end, a POPM control system with five electrohydraulic partitions is adopted, and a mathematical model of the POPM is derived. In addition, a mathematical model of each partition of the electrohydraulic system is derived for the telescope controller design. A linear active disturbance rejection controller (LADRC) and a sliding mode controller (SMC) are adopted in each electro-hydraulic partition to ensure positioning accuracy. Experiments are carried out on 4 and 1.2 m large telescopes. The corresponding results show that by both keeping constant and varying the elevation of the large telescopes, the position error of the primary mirror can be limited to less than 1 μm, and the orientation of the primary mirror can be maintained with an error of less than 0.05 arcsec, even in the presence of external disturbances. This control accuracy can guarantee both the inalterability of the optical axis of the primary mirror and the possibility to adjust the light according to the requirement in order to obtain high-resolution images.

Modal identification of time-varying vehicle-bridge system using a single sensor

Wen-Yu He,Yilin Li,Wei-Xin Ren,Junfei Li,Zhi-Wei Chen 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.30 No.1

Modal parameters are widely used in bridge damage detection, finite element model (FEM) updating and design optimization. However, the conventional modal identification approaches require large number of sensors, enormous data processing workload, but normally result in mode shapes with low accuracy. This paper proposes a modal identification method of time-varying vehicle-bridge system using a single sensor. Firstly, the essential physical relationship between the instantaneous frequency of the vehicle-bridge system and the bridge mode shapes are derived. Subsequently, based on the synchroextracting transform, the instantaneous frequency of the system is tracked through the dynamic response collected by a single sensor, and further the modal parameters are estimated by using the derived physical relationship. Then numerical and experimental examples are conducted to examine the feasibility and effectiveness of the proposed method. Finally, the modal parameters identified by the proposed method are applied in bridge FEM updating. The results manifest that the proposed method identifies the modal parameters with high accuracy via a single sensor, and can provide reliable data for the FEM updating.

The Impact of Health Promotion Activities on the Physiological, Psychological, and Social Functions of Inpatients With Chronic Mental Illness

Yu Li Lan,Lieh Yung Ping,Li Wen Su,Chien Chih Chen 대한신경정신의학회 2022 PSYCHIATRY INVESTIGATION Vol.19 No.3

Objective To explore the impact of the participation of patients with chronic mental illness in health promotion activities on their physical, psychological, and social functions.Methods This study included inpatient with chronic mental illness from a hospital in Eastern Taiwan. According to the experimental research design, the selected subjects were randomly divided into a health promotion group and a control group, with 60 people in each group. The health promotion team conducts health promotion activities twice a week, each for approximately 50 minutes, over the course of eight weeks (16 times in total). The measurement instrument adopts the Adult Mental Health Scale (AMHS) scale. Five experts and scholars in related fields are invited to conduct expert validity.Results Using independent sample t-tests to analyze the changes in the two groups after 8 weeks, the physical, psychological and social problems of the health promotion group were significantly improved compared with the control group.Conclusion Health promotion activities can alleviate the physical illness, anxiety, irritability, depression, and social distress of chronic hospitalized mental patients, and can also increase their positive and optimistic mood. It can provide chronic mental health care institutions to plan patients’ physical activities or exercises.

Role of Grain Refinement Mechanism on Microstructure and Performance in AZ31B Alloy During Interactive Alternating Forward Extrusion (AFE) Process

Yu Wang,Feng Li,Ye Wang,Qiang Chen,Xue Wen Li,Wen Bin Fang 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.4

In this study, interactive alternate forward extrusion (AFE) experiments with varying number of extrusion passes of AZ31magnesium alloy were carried out. The evolution of microstructure, and texture and their effects on the mechanical propertieswere studied. The results show that with the increase of loading passes, the grains are significantly refined, and thedynamic recrystallisation (DRX) structure is uniformly distributed in the product, which is the main reason for the increasein microhardness, compressive strength and failure strain. Further, slip and twinning induced DRX behaviour are consideredto be the main deformation methods in the early stage of deformation. Whereas continuous DRX is considered to be the maindeformation methods for the change in grain morphology in the later stage. During the extrusion process, the deflection angleof the base pole decreases. Finally, the fibre texture is formed; the texture strength is significantly reduced because of theeffect of recrystallisation. The AZ31 magnesium alloy interactive AFE process is discussed with respect to the technologicalexperiment and the microstructure deformation, thus providing a vital scientific basis for further application.

Identification of QTLs associated with heat tolerance at the heading and flowering stage in rice (Oryza sativa L.)

Li, Mao-mao,Li, Xia,Yu, Li-qin,Wu, Jin-wen,Li, Hui,Liu, Jin,Ma, Xiao-ding,Jo, Su-min,Park, Dong-Soo,Song, Youchun,Shin, Dongjin,Han, Long-zhi Springer-Verlag 2018 Euphytica Vol.214 No.4

Ortho-topolin riboside induces apoptosis in Acute myeloid leukemia HL-60 cells

Li Wang,Dong Li Yu,Han Wen Zhang,Lei Yu He,Lei Wu,Li Wang 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.2

6-(2-hydroxybenzylamino)-9-D-ribofuranosylpurine (ortho-topolin riboside, oTR), a naturally occurring cytokinin and nucleoside analog has potential anticancer effects. However, the molecular mechanisms remain elusive. We found that oTR strongly inhibited Acute myeloid leukemia HL-60 cell proliferation, altered the cell cycle, induced cytochrome c release from mitochondria into the cytosol, and increased caspase-3 activity. Apoptosis was confirmed by DNA ladder formation following gel electrophoresis. These results indicated that oTR induced apoptosis through activation of the intrinsic mitochondrial pathway. Moreover, the apoptosis was significantly suppressed by the adenosine transporter inhibitor dipyridamole and adenosine kinase inhibitor A-134974. These data indicated that cellular uptake of oTR was an active process involving an adenosine transporter, and subsequently phosphorylated by an adenosine kinase. Taken together, Our study suggests that oTR is taken up by HL-60 cells, converted to the phosphorylated form, and induces apoptosis.