Development of high-quality soybean cultivars using genome sequence information

Suk-Ha Lee,Kyujung Van,Moon Young Kim,Yeong-Ho Lee,Yangjae Kang 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The purposes of this research project are to identify quantitative trait loci (QTLs) associated with yield-related traits using a recombinant inbred line (RIL) population derived from a cross between a high-yield soybean genotype SS0404-T5-76 and Daewonkong and to develop high-yield soybean and lodging-resistant sprout soybean cultivars. For development of DNA markers and identification of functional sequence variations, firstly, whole genome of five soybean genotypes, Sinpaldalkong 2, SS2-2, Pungsanamulkong, SS0404-T5-76 and Daewongkong, were sequenced using Illumina Hi-Seq technology. SS2-2 is a EMS-induced mutant of Sinpadalkong 2. SS0404-T5-76 showing high-yield is a F8 RIL derived from a cross of Pungsanamulkong x SS2-2. Daewonkong is a elite cultivar with high-protein. Furthermore, to construct a genetic linkage map, we are advancing F4 lines of SS0404-T5-76 x Daewonkong by single seed-descent. Secondly, we developed high-protein and high-yield soybean lines and lodging-resistant sprout lines. Area-adaptability tests of these promising lines are performing in three different locations including Jeju, Naju, and Suwon. Based on the results of area adaptability tests, we are planing to conduct cultivar registration of the promising soybean lines.

Effect of Gyro Sensor to the Position Error in Mobile Robot

Kyujung Lee,Choong Ho Lee 보안공학연구지원센터 2015 International Journal of Software Engineering and Vol.9 No.3

A mobile robot generates position error when it changes directions. To reduce the position error a gyro sensor can be used. However, the effect of gyro sensor is not fully measured precisely for various conditions. This paper focuses on the effect of gyro sensor by measuring position error which is comprised of angular error and distance error. In the experimentation, the position errors of mobile robots for five different courses are measured for the robot with a gyro sensor and those without it. The closed courses include three, five, seven, nine and eleven direction changes which cause to generate considerable position errors. The experimental result shows that the angular error is reduced to about 14.3%~41.4% after a gyro sensor is used. Further, the position errors in distance are reduced to 22.4~31.8% when a gyro sensor is used. The remarkable difference between the two kind of errors is that the angular error increases steadily whereas the distance error increases rapidly but does not increase anymore over a certain level as the number of direction change increases.

변형속도에 따른 M1 마그네슘 합금의 고온변형 중 미세조직 형성 거동

이규정 ( Kyujung Lee ),김권후 ( Kwonhoo Kim ) 한국열처리공학회 2019 熱處理工學會誌 Vol.32 No.1

In this study, microstructure evolution and crystallographic orientation are investigated under various deformation conditions in M1 magnesium alloy. M1 magnesium ingot was rolled at 673 K with the rolling reduction of 30%. The compression test specimens were machined out from rolled plate, and then the specimens were annealed at 823 K for 1h. Uniaxial compression tests were conducted at 723 K and under the strain rate ranging from 5.0 × 10^-4 s^-1 to 5.0 × 10^-2 s^-1 up to a true strain of -1.0. For observation of crystal orientation distribution, EBSD measurement was performed. Occurrence of the dynamic recrystallization and grain boundary migration were confirmed in all case of the specimens. The distribution of the grains is not uniformed in the experimental conditions. (Received January 2, 2019; Revised January 15, 2019; Accepted January 22, 2019)

Association analysis between genomic region of isoflavone reductase gene and maturity in soybean

Solmoe Lee,Myounghai Kwak,Kyujung Van,Moon Young Kim,Suk-Ha Lee 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

The control of earliness has an agronomic importance since it reduces growing and harvesting time. Earliness is controlled by multiple genes in multiple pathways and influenced by the environment. In Arabidopsis thaliana, many earliness related genes were identified. Among them, Arabidopsis Frigida (FRI) gene confers late flowering phenotype, which is reversed to earliness by vernalization. Blast search using FRI against soybean EST database at TIGR identified Isoflavone reductase-like gene (TC217830). Fifty seven SNPs were identified in a total of 4,242 bp lengths in genomic region of Isoflavone reductase-like from 62 soybean genotypes (31 early maturity group and 31 late maturity group). From the obtained sequences, we identified 6 haplotypes of Isoflavone reductase-like gene. Among them, three haplotypes showed a significant association with maturity, suggesting that Isoflavone reductase-like gene is tightly linked to flowering time or actual gene it-self. Thus, to delimit a putative genomic region for maturity and flowering time, SSR markers near Isoflavone reductase-like gene were designed and analyzed for their genetic diversity, assuming that highly selected regions might posses lower genetic diversity. Through these experiments, the region related to maturity and flowering was delimited to nearby ac_satc_4 in scaffold 16.

전 방향 이동장치를 이용한 홀로노믹 구현과 색상 영상을 이용한 목표물 추적 알고리즘 연구

이규정(Kyujung Lee),이충호(Choong Ho Lee) 한국정보과학회 2012 한국정보과학회 학술발표논문집 Vol.39 No.2D

Soybean cultivars screening for resistance to Phomopsis seed decay in Korea

Suli Sun,Kyujung Van,Moon Young Kim,Yeong-Ho Lee,Ahra Bae,Hyun Ju Jang,Suk-Ha Lee 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Phomopsis seed decay (PSD), primarily caused by Phomopsis longicolla, is a major contributor to poor soybean seed quality and significant yield loss, particularly in early maturing soybean genotypes. However, it is not yet known whether PSD resistance is associated with early maturity. This study was conducted to identify quantitative trait loci (QTLs) for resistance to PSD and maturity time using a recombinant inbred line (RIL) population derived from a cross between the PSD-resistant Taekwangkong and the PSD-susceptible SS2-2. Based on a genetic linkage map incorporating 117 simple sequence repeat markers, QTL analysis revealed two and three QTLs conferring PSD resistance and maturity time, respectively, in the RIL population. Two QTLs (PSD-6-1 and PSD-10-2) for PSD resistance were identified in the intervals of Satt100-Satt460 and Sat_038-Satt243 on chromosomes (Chrs) 6 and 10, respectively. These QTLs do not overlap with any previously reported loci for PSD resistance in other soybean genotypes. Two QTLs explained phenotypic variances in PSD resistance of 46.3% and 14.1%, respectively. Among three QTLs for maturity time, two (Mat-6-2 and Mat-10-3) were located at positions similar to the PSD resistance QTLs. The identification of the QTLs linked to both PSD resistance and maturity time indicates a biological correlation between these two traits. The newly identified QTLs for resistance to PSD associated with maturity time in Taekwangkong will help improve soybean resistance to P. longicolla.

텍스트를 이미지화하기 위한 Interface 제작에 관한 연구 : 작품"Yesterday to Today"를중심으로

이우현(Woohyun Lee),이성영(Sungyoung Lee),김규정(Kyujung Kim) 한국HCI학회 2007 한국HCI학회 학술대회 Vol.2007 No.2

Identification of QTLs Associated with Resistance to Riptortus clavatus Thunberg (Heteroptera: Alydidae) in Soybean (Glycine max L. Merr.)

Li, Wenxin,Van, Kyujung,Zheng, Da-Hao,Liu, Weixian,Lee, Yeong-Ho,Lee, Sue-Yeon,Lee, Joon-Ho,Lee, Suk-Ha The Korean Society of Crop Science 2008 Journal of crop science and biotechnology Vol.11 No.4

The bean bug Riptortus clavatus Thunberg (Heteroptera: Alydidae) is an important pest, causing serious yield loss in soybean. But the information on mechanism of resistance to R. clavatus is limited. The objective of this study was to identify QTLs for R. clavatus resistance using simple sequence repeat (SSR) markers in a soybean population of recombinant inbred lines (RILs) developed from the cross PI 171451 ${\times}$ Hwaeomputkong. A genetic map from this population was constructed with a total of 136 SSR markers covering 1073.9 cM on 20 linkage groups (LGs). With 126 $F_5$ RILs, two independent QTLs for resistance to R. clavatus were mapped on LGs B1 and C2. The amount of phenotypic variation explained by these QTLs ranged from 12 to 16%. PI 171451 showed an escape response to R. clavatus. Under feeding conditions, 14.4% of RILs showed greater resistance to R. clavatus than the resistant parent. The resistance to R. clavatus in soybean from PI 171451 was incomplete and quantitatively inherited and the QTLs for resistance to R. clavatus detected in the RIL population were not significantly affected by epistatic interactions.

Genetic Variation in Sprout-related Traits and Microsatellite DNA Loci of Soybean

Kyujung Van,Moon Young Kim,Jae-Gyun Gwag,Kyung-Geun Bae,Young Jin Oh,Kyong Ho Kim,Ho Ki Park,Suk-Ha Lee 韓國作物學會 2003 Korean journal of crop science Vol.48 No.5

Genetic diversity and soybean sprout-related traits were evaluated in a total of 72 soybean accessions (60 Glycine max, 7 Glycine soja, and 5 Glycine gracilis). 100-seed weight (SW) was greatly varied and ranged from 3.2g to 32.3g in 72 soybean accessions. Positive correlation was observed between GR and hypocotyl length (HL), whereas negative correlation was observed between SW and hypocotyl diameter (HD). Re-evaluation by discarding two soybean genotypes characterized with low GR indicated that much higher correlation of sprout yield (SY) with HD and SW. Based on the principal component analysis (PCA) for sprout-related traits, 57 accessions were classified. Soybean genotypes with better traits for sprout, such as small size of seeds and high SY, were characterized with high PCA 1 and PCA 2 values. The seed size in second is small but showed low GR and SY, whereas the third has large seed, high GR and more than 400% SY. In genetic similarity analysis using 60 SSR marker genotyping, 72 accessions were classified into three major and several minor groups. Nine of twelve accessions that were identified as the representatives of soybean for sprout based on PCA were in a group by the SSR marker analysis, indicating the SSR marker selection of parental genotypes for soybean sprout improvement program.

Rapid and real-time diagnosis of hypoalbuminemia using an extraordinary optical transmission biosensor

Lee, Yeji,Song, Hyerin,Ahn, Heesang,Choi, Jong-ryul,Kim, Kyujung Elsevier 2018 Sensors and actuators. B Chemical Vol.274 No.-

<P><B>Abstract</B></P> <P>A hypoalbuminemia diagnosis kit, based on an extraordinary optical transmission sensor, for accurate and fast measurements of albumin concentrations in human serum was demonstrated. First, using nanohole arrays, the sensitivity of the sensor was confirmed to be approximately 460 nm/RIU by theoretical simulations and using standard solutions with specific refractive indices. In the preliminary study of albumin measurements, non-labeled detection of a difference in albumin concentration of approximately 10 mg/ml was achieved with a spectral peak shift of 0.8972 nm and proved to be capable of diagnosing hypoalbuminemia without a surface modification process. Additionally, we successfully classified 100 samples with different concentrations into normal and hypoalbuminemia groups with a probability greater than 98%. According to the results, the hypoalbuminemia diagnosis kit has a potential to be practically employed for real-time, preclinical, label-free diagnosis with integration of advanced microfluidics and miniaturized optics.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A hypoalbuminemia diagnosis kit based on an EOT sensor was demonstrated. </LI> <LI> The sensitivity of the sensor with nanohole arrays is 463.6 nm/RIU. </LI> <LI> Label free detections for the difference of albumin concentrations about 10 mg/ml was achieved. </LI> <LI> Normal and hypoalbuminemia groups was distinguished with a probability of over 98%. </LI> </UL> </P>