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Kim, Eun-A,Kim, Seo-Young,Ye, Bo-Ram,Kim, Junseong,Ko, Seok-Chun,Lee, Won Woo,Kim, Kil-Nam,Choi, Il-Whan,Jung, Won-Kyo,Heo, Soo-Jin Elsevier 2018 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.59 No.-
<P><B>Abstract</B></P> <P>In this study, we confirmed the anti-inflammatory effect of Apo-9-fucoxanthinone (AF) in <I>in vitro</I> RAW 264.7 cells and <I>in vivo</I> zebrafish model. In lipopolysaccharide (LPS)-stimulated zebrafish, AF significantly decreased the production of reactive oxygen species (ROS), nitric oxide (NO) and cell death. In addition, the mRNA expression of inducible nitric oxide synthase (iNOS), suppressed cyclooxygenase-2 (COX-2) and an inflammatory cytokines; IL-1β, TNF-α were shown reduction. And AF significantly inhibited NO production and expression of iNOS in LPS-stimulated RAW 264.7 cells. Further, AF suppressed COX-2, prostaglandin E2 (PGE<SUB>2</SUB>), and pro-inflammatory cytokines such as interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) at 25, 50 and 100 μg/mL, respectively. Further mechanistic studies showed that AF suppressed the nuclear factor-kB (NF-kB) pathway and phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). According to the results, AF can be used and applied as a useful anti-inflammatory agent of nutraceutical or pharmaceutical.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Anti-inflammatory effect of Apo-9-fucoxanthinone in <I>in vitro</I> RAW 264.7 cells and <I>in vivo</I> zebrafish </LI> <LI> Apo-9-fucoxanthinone suppressed NO production through NF-kB and MAPKs pathway. </LI> <LI> In LPS-stimulated zebrafish, Apo-9-fucoxanthinone significantly decreased ROS, NO, cell death and pro-inflammatory cytokines. </LI> <LI> Apo-9-fucoxanthinone can be extremely useful as an effective anti-inflammatory agent. </LI> </UL> </P>
SoxF Transcription Factors Are Positive Feedback Regulators of VEGF Signaling
Kim, Kangsan,Kim, Il-Kug,Yang, Jee Myung,Lee, Eunhyeong,Koh, Bong Ihn,Song, Sukhyun,Park, Junseong,Lee, Sungsu,Choi, Chulhee,Kim, Jin Woo,Kubota, Yoshiaki,Koh, Gou Young,Kim, Injune Grune & Stratton 2016 Circulation research Vol.119 No.7
<P>Rationale: Vascular endothelial growth factor (VEGF) signaling is a key pathway for angiogenesis and requires highly coordinated regulation. Although the Notch pathway-mediated suppression of excessive VEGF activity via negative feedback is well known, the positive feedback control for augmenting VEGF signaling remains poorly understood. Transcription factor Sox17 is indispensable for angiogenesis, but its association with VEGF signaling is largely unknown. The contribution of other Sox members to angiogenesis also remains to be determined. Objective: To reveal the genetic interaction of Sox7, another Sox member, with Sox17 in developmental angiogenesis and their functional relationship with VEGF signaling. Methods and Results: Sox7 is expressed specifically in endothelial cells and its global and endothelial-specific deletion resulted in embryonic lethality with severely impaired angiogenesis in mice, substantially overlapping with Sox17 in both expression and function. Interestingly, compound heterozygosity for Sox7 and Sox17 phenocopied vascular defects of Sox7 or Sox17 homozygous knockout, indicating that the genetic cooperation of Sox7 and Sox17 is sensitive to their combined gene dosage. VEGF signaling upregulated both Sox7 and Sox17 expression in angiogenesis via mTOR pathway. Furthermore, Sox7 and Sox17 promoted VEGFR2 (VEGF receptor 2) expression in angiogenic vessels, suggesting a positive feedback loop between VEGF signaling and SoxF. Conclusions: Our findings demonstrate that SoxF transcription factors are indispensable players in developmental angiogenesis by acting as positive feedback regulators of VEGF signaling.</P>
Copper-Catalyzed Selective Arylations of Benzoxazoles with Aryl Iodides
Kim, Donghae,Yoo, Kwangho,Kim, Se Eun,Cho, Hee Jin,Lee, Junseong,Kim, Youngjo,Kim, Min American Chemical Society 2015 Journal of organic chemistry Vol.80 No.7
<P>A copper-catalyzed direct ring-opening double N-arylation of benzoxazoles with aryl iodides has been developed. The present system exhibits high selectivity despite competition from C-arylation. The selectivity between ring-opening N-arylation and C-arylation was controlled by the choice of reaction vessel. The nitrile bound bis(triphenylphosphine)copper cyanide was identified as the active catalytic species for both reactions, and when combined with a nitrile-containing solvent, enhanced the reaction efficiency.</P>
( Junseong Kim ),( Youn Kyung Choi ),( Ji-hyeok Lee ),( Seo-young Kim ),( Hyun-soo Kim ),( You-jin Jeon ),( Soo-jin Heo ) 한국키틴키토산학회 2017 한국키틴키토산학회지 Vol.22 No.3
Red tide Heterosigma akashiwo (H. akashiwo), a microscopic alga of the class Raphidophyceae, causes extensive damage to all marine ecosystems. It is essential to reduce the damage to marine ecosystems for them to be used as a resource. In this study, we used organic solvent fractionation to obtain an ethyl acetate-methanol extract from H. akashiwo (HAEM80) and then evaluated its anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and a zebrafish model. HAME80 markedly inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2). It also down-regulated the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and decreased the secretion of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. HAME80 reduced yolk edema and improved the survival rate of LPS-stimulated zebrafish embryos; in addition, the extract significantly reduced the production of ROS and NO and attenuated cell death in this model. Gas chromatography-mass spectrometry (GC-MS) of the extract was used to confirm the identity of peaks 1-20. Taken together, our data suggest that H. akashiwo is a beneficial anti-inflammatory agent.
Kwak, Jiyong,Shim, Jin-Kyoung,Kim, Dong Seok,Lee, Ji-Hyun,Choi, Junjeong,Park, Junseong,Shin, Kyoung-Jin,Kim, Se-Hoon,Kim, Pilnam,Huh, Yong-Min,Kim, Eui Hyun,Chang, Jong Hee,Kim, Sun Ho,Kang, Seok-Gu Spandidos Publications 2016 International journal of oncology Vol.49 No.2
<P>The existence of tumorspheres (TSs) might confer treatment resistance to pineoblastoma (PB). The existence of PB TSs with cellular immortalization potential has not yet been reported. We developed a procedure for isolating TSs from recurrent PB (rPB) and tested whether their properties made them suitable for use as a patient-derived xenograft (PDX). Immunocytochemical staining, RT-PCR and quantitative real-time PCR showed that, among stemness proteins, CD133, musashi and podoplanin were expressed at elevated levels in rPB TSs, but nestin was not. rPB TSs cultured under neuro-glial differentiation conditions expressed TUBB3, but not GFAP, MBP or NeuN. Unlike glioblastoma TSs, rPB TSs showed no clear evidence of invasion in 3D invasion assay or increased expression of genes associated with epithelial-mesenchymal transition. An orthotopic xenograft showed that tumor xenografts replicated the histopathological features of the patient tumor and expressed similar genome profiles, as determined by short tandem repeat genotyping. These data demonstrate the isolation and the characterization of rPB TSs for the first time. Using an orthotopic xenograft, we showed that rPB TSs could replicate the patient tumor, demonstrating their potential as a PDX for precision medicine.</P>
Zirconocene Complexes as Catalysts for the Cycloaddition of CO<sub>2</sub> to Propylene Oxide
Kim, So Han,Ahn, Duseong,Kang, Yi Young,Kim, Min,Lee, Ki‐,Soo,Lee, Junseong,Park, Myung Hwan,Kim, Youngjo WILEY‐VCH Verlag 2014 European journal of inorganic chemistry Vol.2014 No.30
<P><B>Abstract</B></P><P>Six zirconocene derivatives were systematically designed; one of the cyclopentadienyl (Cp) ligands of zirconocene dichloride [Cp<SUB>2</SUB>ZrCl<SUB>2</SUB>] was silylated and this group was then incrementally increased in size; furthermore, one derivative with a 4,4′‐disilylbiphenyl bridge between the two zirconocene fragments was prepared. One zirconium complex was characterized by single‐crystal X‐ray analysis. All zirconium complexes were effective catalysts for the cycloaddition of CO<SUB>2</SUB> to propylene oxide. Two complexes containing a biphenyl group attached to a SiMe<SUB>2</SUB> group showed higher activity than the other complexes. In addition, the dinuclear complex containing the biphenylenebis(dimethylsilyl) bridge showed the highest activity among all the zirconium complexes reported. Furthermore, these complexes are the first examples of zirconocene catalytic systems for the cycloaddition reaction of CO<SUB>2</SUB> to propylene oxide.</P>
A Case Study on Network Status Classification based on Latency Stability
( Junseong Kim ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.11
Understanding network latency is important for providing consistent and acceptable levels of services in network-based applications. However, due to the difficulty of estimating applications` network demands and the difficulty of network latency modeling the management of network resources has often been ignored. We expect that, since network latency repeats cycles of congested states, a systematic classification method for network status would be helpful to simplify issues in network resource managements. This paper presents a simple empirical method to classify network status with a real operational network. By observing oscillating behavior of end-to-end latency we determine networks` status in run time. Five typical network statuses are defined based on a long-term stability and a short-term burstiness. By investigating prediction accuracies of several simple numerical models we show the effectiveness of the network status classification. Experimental results show that around 80% reduction in prediction errors depending on network status.
Dinuclear Aluminum Complexes as Catalysts for Cycloaddition of CO<sub>2</sub> to Epoxides
Kim, So Han,Ahn, Duseong,Go, Min Jeong,Park, Myung Hwan,Kim, Min,Lee, Junseong,Kim, Youngjo American Chemical Society 2014 Organometallics Vol.33 No.11
<P>The synthesis and characterization of dinuclear aluminum complexes bearing multidentate aliphatic aminoethanol-based ligands are presented. Single-crystal X-ray analyses, NMR data, and mass data reveal that four aluminum complexes synthesized are all dimeric in the solid, solution, and gas states. Especially, <SUP>27</SUP>Al NMR spectra have demonstrated that they exist as both five-coordinate Al(III) species in benzene-<I>d</I><SUB>6</SUB> solution. All aluminum complexes are effective catalysts for the cycloaddition of CO<SUB>2</SUB> to propylene oxide in the presence of <I>n</I>-Bu<SUB>4</SUB>NI as a cocatalyst. Complexes <B>1</B> and <B>3</B>, which have two methyl groups per aluminum center, are better catalytic systems than the corresponding complexes <B>2</B> and <B>4</B> with a mono methyl group per aluminum. In addition, complexes <B>3</B> and <B>4</B> containing a pendant −CH<SUB>2</SUB>CH<SUB>2</SUB>NMe<SUB>2</SUB> group attached to nitrogen showed the higher activity than those <B>1</B> and <B>2</B> with a pendant −CH<SUB>2</SUB>CH<SUB>2</SUB>OMe group did. As expected, the catalytic activity for <B>3</B> increases as the reaction temperature increases up to 130 °C. In addition, compound <B>3</B> showed the highest activity for the cycloaddition of CO<SUB>2</SUB> with propylene oxide in the presence of tetrabutylphosphonium bromide as a cocatalyst.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/orgnd7/2014/orgnd7.2014.33.issue-11/om500257j/production/images/medium/om-2014-00257j_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/om500257j'>ACS Electronic Supporting Info</A></P>