BF-30 effectively inhibits ciprofloxacin-resistant bacteria in vitro and in a rat model of vaginosis

Wang, Jing,Li, Bing,Li, Yang,Dou, Jie,Hao, Qingru,Tian, Yuwei,Wang, Hui,Zhou, Changlin 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.7

Bacterial infections are becoming increasingly difficult to treat due to the increasing number of multidrug-resistant strains. Cathelicidin-BF (BF-30) is a cathelicidin-like antimicrobial peptide and exhibits broad antimicrobial activity against bacteria. In the present study, the antibacterial activity of BF-30 against ciprofloxacin-resistant Escherichia coli and Staphylococcus aureus was examined, and the protective effects of this peptide against these bacteria in rats with bacterial vaginosis were identified for the first time. The data showed that BF-30 had effective antimicrobial activities against ciprofloxacin-resistant E. coli and S. aureus. The minimal inhibitory concentrations for both bacterial strains were $16{\mu}g/ml$, and the minimal bactericidal concentrations were 64 and $128{\mu}g/ml$, respectively. A time course experiment showed that the CFU counts rapidly decreased after BF-30 treatment, and the bacteria were nearly eliminated within 4 h. BF-30 could reduce the fold change (CFU/ml) in local colonization by drug-resistant E. coli and S. aureus to 0.01 at a dose of 0.8 mg/kg/day in the rats' vaginal secretions. In addition, BF-30 induced membrane permeabilization and bound to the genomic DNA, interrupting protein synthesis. Taken together, our data demonstrate that BF-30 has potential therapeutic value for the prevention and treatment of bacterial vaginosis.

BF-30 effectively inhibits ciprofloxacin-resistant bacteria in vitro and in a rat model of vaginosis

Jing Wang,Bing Li,Yang Li,Jie Dou,Qingru Hao,Yuwei Tian,Hui Wang,Changlin Zhou 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.7

Bacterial infections are becoming increasinglydifficult to treat due to the increasing number of multidrugresistantstrains. Cathelicidin-BF (BF-30) is a cathelicidinlikeantimicrobial peptide and exhibits broad antimicrobialactivity against bacteria. In the present study, the antibacterialactivity of BF-30 against ciprofloxacin-resistantEscherichia coli and Staphylococcus aureus was examined,and the protective effects of this peptide against these bacteriain rats with bacterial vaginosis were identified for thefirst time. The data showed that BF-30 had effective antimicrobialactivities against ciprofloxacin-resistant E. coliand S. aureus. The minimal inhibitory concentrations forboth bacterial strains were 16 lg/ml, and the minimal bactericidalconcentrations were 64 and 128 lg/ml, respectively. A time course experiment showed that the CFUcounts rapidly decreased after BF-30 treatment, and thebacteria were nearly eliminated within 4 h. BF-30 couldreduce the fold change (CFU/ml) in local colonization bydrug-resistant E. coli and S. aureus to 0.01 at a dose of0.8 mg/kg/day in the rats’ vaginal secretions. In addition,BF-30 induced membrane permeabilization and bound to thegenomic DNA, interrupting protein synthesis. Taken together,our data demonstrate that BF-30 has potential therapeuticvalue for the prevention and treatment of bacterialvaginosis.

Adaptive-and-Resolvable Fractional Repetition Codes Based on Hypergraph

Tiantian Wang,Jing Wang,Haipeng Wang,Jie Meng,Chunlei Yu,Shuxia Wang 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.4

Fractional repetition (FR) codes can achieve exact uncoded repair for multiple failed nodes, with lower computational complexity and bandwidth overhead, and effectively improve repair performance in distributed storage systems (DSS). The actual distributed storage system is dynamic, that is, the parameters such as node storage overhead and number of storage nodes will change randomly and dynamically. Considering that traditional FR codes cannot be flexibly applied to dynamic distributed storage systems, a new construction scheme of adaptive-and-resolvable FR codes based on hypergraph coloring is proposed in this paper. Specifically, the linear uniform regular hypergraph can be constructed based on the heuristic algorithm of hypergraph coloring proposed in this paper. Then edges and vertices in hypergraph correspond to nodes and coded packets of FR codes respectively, further, FR codes is constructed. According to hypergraph coloring, the FR codes can achieve rapid repair for multiple failed nodes. Further, FR codes based on hypergraph coloring can be generalized to heterogeneous distributed storage systems. Compared with Reed-Solomon (RS) codes, simple regenerating codes (SRC) and locally repairable codes (LRC), adaptive-and-resolvable FR codes have significant advantages over repair locality, repair bandwidth overhead, computational complexity and time overhead during repairing failed nodes.

Prognostic Significance of Interactions Between ER Alpha and ER Beta and Lymph Node Status in Breast Cancer Cases

Han, Shu-Jing,Guo, Qing-Qing,Wang, Ting,Wang, You-Xin,Zhang, Yu-Xiang,Liu, Fen,Luo, Yan-Xia,Zhang, Jie,Wang, You-Li,Yan, Yu-Xiang,Peng, Xiao-Xia,Ling, Rui,He, Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Objective: Both estrogen receptors, ER alpha ($ER{\alpha}$) and ER beta ($ER{\beta}$), are expressed in 50-70% of breast cancer cases. The role of $ER{\alpha}$ as a prognostic marker in breast cancer has been well established as its expression is negative correlated with tumor size and lymph node metastasis. $ER{\beta}$ is also a favorable prognostic predictor although this is less well documented than for $ER{\alpha}$. Materials and Methods: To explore whether ERs independently or together might influence clinical outcome in breast cancer, the correlation between the ERs with the clinicopathological features was analyzed in 84 patients. Results: $ER{\alpha}$ expression negatively correlated with tumor stage (r=-0.246, p=0.028) and tended to be negatively correlated with lymph node status (r=-0.156, p=0.168) and tumor size (r=-0.246, p=0.099). Also, $ER{\beta}$ was negatively correlated with nodal status (r=-0.243, p=0.028), as was coexpression of $ER{\alpha}$ and $ER{\beta}$ (p=0.043, OR=0.194, 95% CI= 0.040-0.953). Conclusion: Coexpression of ERs might serve as an indicator of good prognosis in breast cancer patients.

Clinical Study of Thalidomide Combined with Dexamethasone for the Treatment of Elderly Patients with Newly Diagnosed Multiple Myeloma

Chen, Hai-Fei,Li, Zheng-Yang,Tang, Jie-Qing,Shen, Hong-Shi,Cui, Qing-Ya,Ren, Yong-Ya,Qin, Long-Mei,Jin, Ling-Juan,Zhu, Jing-Jing,Wang, Jing,Ding, Jie,Wang, Ke-Yuan,Yu, Zi-Qiang,Wang, Zhao-Yue,Wu, Tian Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Objective: To investigate the relationship between the efficacy and safety of different doses of thalidomide (Thal) plus dexamethasone (Dex) as the initial therapy in elderly patients with newly diagnosed multiple myeloma (MM). Methods: Clinical data of 28 elderly patients with newly diagnosed MM who underwent the TD regimen as the initial therapy were analyzed retrospectively. The patients were divided into two groups according to the maximal sustained dose of Thal: lower dose (group A) and higher dose (group B). The overall response rate (ORR), progression free survival (PFS), overall survival (OS), and adverse events (AES) were compared between the two groups. Results: A total of 28 patients were followed up with a median of 18 months. The ORR was 60.1%. The median response time and PFS were 2.0 and 17.0 months, respectively. The mean sustained dose of Thal in group B was significantly higher than group A (292.9 mg v 180.4 mg, P=0.01). There was no significantly difference in ORR (57.1% v 64.3%, P=1.00) and PFS (9.63months v 17.66 months, P=0.73) between groups A and B. During the follow up, only five patients died (<40%) and, therefore, median OS values were not available. It is estimated, however, that the mean survival time in the two groups was 35.6 and 33.4 months (P>0.05), respectively. All of the patients tolerated the treatment well. The incidence of AES in patients with a grading above 3 in group B was significantly higher than in group A (P=0.033). Conclusions: The TD regimen results in a high response rate and manageable AES as the initial therapy in elderly patients with MM. TD should be considered as the front line regimen for the treatment of elderly patients with MM in areas with financial constraints. The clinical response can be achieved at a low dose Thal with minimal toxicity.

Glulathione-S-transferases Gene Polymorphism in Prediction of Gastric Cancer Risk by Smoking and Helicobacter Pylori Infection Status

Jing, Chen,Huang, Zhi-Jie,Duan, Yu-Qin,Wang, Pei-Hong,Zhang, Ru,Luo, Ke-Shu,Xiao, Xin-Rong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Aim: To evaluate the association of glutathione S-transferases gene polymorphisms with the risk of gastric cancer, with reference to smoking and Helicobacter pylori infection. Methods: We conducted a 1:1 matched case-control study with 410 gastric cancer cases and 410 cancer-free controls. Polymorphisms of GSTM1, GSTT1 and GSTP1 were determined using PCR-CTPP. Results: The GSTM1 and GSTT1 null genotypes were significantly associated with the risk of gastric cancer after adjusting for potential confounding factors (OR=1.68, 95% CI=1.32-2.23 for null GSTM1, OR=1.73; 95% CI=1.24-2.13 for null GSTT1). The combination of null GSTM1 and null GSTT1 conferred an elevated risk (OR=2.54, 95% CI=1.55-3.39). However, no association was found for GSTP1 polymorphism The smoking modified the association of GSTM1 and GSTT1 null genotypes with the risk of gastric cancer. Conclusion: GSTM1 and GSTT1 null genotypes are associated with increased risk of gastric cancer, and smoking modifies the association.

Equivalent Magnetic Circuit Model for Consequent Pole Hybrid Excitation Synchronous Machine with AC Field Control

Jie Wu,Jing Yin,Baixing Zhuang,Mingjie Wang,Jitao Zhang 한국자기학회 2019 Journal of Magnetics Vol.24 No.4

At present, almost all hybrid excitation machines (HEMs) apply DC field current to control the air-gap flux. However, the DC field control mode results in a problem that the capability of field-weakening is not equal to that of field-strengthening. This paper briefly explains the mechanism of asymmetric bidirectional field control capability in present HEMs, proposes a consequent pole hybrid excitation synchronous (CPHES) machine with AC field control mode to solve the asymmetric problem. The structure and principles of CPHES machine are presented. Additionally, the equivalent magnetic circuit model of CPHES machine is derived to reduce the computational complexity of analyzing CPHES structure by using three-dimensional finite element analysis (3DFEA). The proposed equivalent model and 3D-FEA are compared, the results verify the proposed model is basically consistent with the 3D-FEA, and the proposed model is able to reduce the computational complexity greatly.

The inhibitory effect of sodium baicalin on oseltamivir-resistant influenza A virus via reduction of neuraminidase activity

Jing Jin,Yuanjin Chen,Dechuan Wang,Lingman Ma,Min Guo,Changlin Zhou,Jie Dou 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.6

Baicalin was identified as a neuraminidase (NA)inhibitor displaying anti-influenza A virus (IAV) activity. However, its poor solubility in saline has limited its use inthe clinic. We generated sodium baicalin and showed that itexhibited greatly increased solubility in saline. Its efficacyagainst oseltamivir-resistant mutant A/FM/1/47-H275Y(H1N1-H275Y) was evaluated in vitro and in vivo. Resultsshowed that 10 lM of sodium baicalin inhibited A/FM/1/47 (H1N1), A/Beijing/32/92 (H3N2) and H1N1-H275Y inMDCK cells in a dose-dependent manner, with inhibitoryrates of 83.9, 75.9 and 47.7%, respectively. Intravenousadministration of sodium baicalin at 100 mg/kg/d enabledthe survival of 20% of H1N1-H275Y-infected mice. Thetreatment alleviated body weight loss and lung injury. Moreover, sodium baicalin exerted a clear inhibitory effecton NAs. The IC50 values of sodium baicalin against H1N1-H275Y and cells-expressing A/Anhui/1/2013-R294K(H7N9-R294K) NA protein (N9-R294K) were 214.4 lMand 216.3 lM. Direct interactions between sodium baicalinand NA were observed, and we simulated the interactionsof sodium baicalin with N9-R294K and N9 near the activesites of OC-N9-R294K and OC-N9. The residues responsiblefor the sodium baicalin-N9-R294K and sodiumbaicalin-N9 interactions were the same, confirming thatsodium baicalin exerts effects on wild-type and oseltamivir-resistant viral strains.

The microRNA-127-3p directly targeting Vamp2 in C2C12 myoblasts

Jie Li,Gaofu Wang,Jing Jiang,Lin Fu,Peng Zhou,Hangxing Ren 한국통합생물학회 2018 Animal cells and systems Vol.22 No.5

MicroRNAs (miRNAs) have been reported that can regulate skeletal muscle growth and development. Previously, we demonstrated that miR-127-3p were differently expressed in skeletal muscle and muscle cells. However, the molecular mechanism of miR-127-3p regulation of skeletal myogenesis are not well elucidated. In this study, we transfected miR-127-3p into C2C12 cells, and found miR-127-3p induces myogenesis by targeting Vamp2. Moreover, the regulatory mechanism of Vamp2 in myoblasts proliferation and differentiation was further confirmed. In conclusion, our data providedevidences that miR-127-3p reciprocally regulated myoblasts proliferation and differentiation through directly targeting Vamp2.

Imaging Cell Surface Glycans ofCardiomyocytes in Intact Rat Heart

Jie Rong,Jing Han,Lianshun Feng,Yanhong Tan,Qiwei Wang,Yingying Chen,Shiqiang Wang,Xing Chen 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

Glycosylation is essential for proper cell signaling and embryonic development. Changes in glycosylation are often a hallmark of disease states. Cardiovascular disease has become one of the top causes of death. Many studies using rat models have related heart diseases and regulations of heart physiological functions to altered glycosylations. However, the dynamic regulation and molecular mechanism of glycosylation in heart are not clear. Rat is an important model organism for human cardiovascular disease studies. Isolated intact rat hearts are often used in physiological and pathological studies. In situ imaging biomolecules in intact heart avoid possible damage of myocyte and biased loss of cardiac myocytes during enzymatic isolation. Here, we applied the metabolic glycan labeling technique for imaging sialylation and O-linked glycosylation in living cardiomyocytes and intact rat hearts. The effects of unnatural sugars on the function of cardiomyocytes and hearts were evaluated. We found unnatural sugars did not perturb the function of cardiomyocytes and hearts. Glycan imaging revealed an interesting distribution pattern: sialic acid or O- linked glycan enriched at intercalated discs of adult rat cardiomyocytes. We also observed that the glycosylations of cardiomyocytes were altered in isoproterenol-induced cardiac hypertrophy rat models. This work extends the application of metabolic glycan labeling technique to probe glycosylations in rats and provides the first example of using this technique for in situ cellular glycans imaging in mammalian model organisms. The biological significance of altered glycosylations in cardiac hypertrophy heart is under investigation in our lab.