Rapid Detection and Monitoring Therapeutic Efficacy of Mycobacterium tuberculosis Complex Using a Novel Real-Time Assay

( Jiang Li Juan ),( Wen Juan Wu ),( Hai Wu ),( Son Sik Ryang ),( Jian Zhou ),( Wei Wu ),( Tao Li ),( Jian Guo ),( Hong Hai Wang ),( Shui Hua Lu ),( Yao Li ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9

We combined real-time RT-PCR and real-time PCR (R/P) assays using a hydrolysis probe to detect Mycobacterium tuberculosis complex (MTBC)-specific 16S rRNA and its rRNA gene (rDNA). The assay was applied to 28 nonrespiratory and 207 respiratory specimens from 218 patients. Total nucleic acids (including RNA and DNA) were extracted from samples, and results were considered positive if the repeat RT-PCR threshold cycle was ≤35 and the ratio of real-time RT-PCR and real-time PCR load was ≥1.51. The results were compared with those from existing methods, including smear, culture, and real-time PCR. Following resolution of the discrepant results between R/P assay and culture, the overall sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV) of all samples (including nonrespiratory and respiratory specimens) were 98.2%, 97.2%, 91.7%, and 99.4%, respectively, for R/P assay, and 83.9%, 89.9%, 72.3%, and 94.7%, respectively, for real-time PCR. Furthermore, the R/P assay of four patient samples showed a higher ratio before treatment than after several days of treatment. We conclude that the R/P assay is a rapid and accurate method for direct detection of MTBC, which can distinguish viable and nonviable MTBC, and thus may guide patient therapy and public health decisions.

The Prediction Research of Population Density Based on Deep Learning in Grain Stored Insects

Wu Jian-Jun,Dang Hao,Li Miao,Sun Fu-Yan,Zhu Yu-Hua,Zhen Tong,Zou Bing-Qiang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.10

Precision of pests, in stored grain insect population density, has been a hot and difficult research in pest detection and control system. The accuracy of prediction of pest density will directly affect to warehouse grain temperature and the food quality etc. In order to improve the accuracy, the paper which using the depth study method, established an insects density prediction mode with the depth of the belief network as the core. The model is applied to the algorithm of deep learning predictive control. According to the temperature and humidity of the grain obtained from the actual measurement and the initial density of the pest, we predicted the pest density. Simulation results show that the root mean square error is small between the predictive value and actual value, high prediction accuracy. The deep learning algorithm is applied to the population density of pests is effective.

Cutting performance and wear mechanism of WC-Co ultrafine cemented carbide during cutting of HT250 gray cast iron

Jian Chen,Man Feng Gong,Shang Hua Wu 한양대학교 세라믹연구소 2015 Journal of Ceramic Processing Research Vol.16 No.2

WC-3TiN-0.8Cr3C2-8Co cemented carbides were sintered in de-nitriding and nitriding conditions to use as the cutting tools for HT250 gray cast iron, respectively. Their microstructures and mechanical properties were studied, and the influence of feed rate on tool wear mechanism was investigated. Tool wear mechanism was analyzed by SEM and EDS. Denitriding cemented carbides with the same composition and grain size were prepared for comparison. The results showed that the inserts of nitriding WC-3TiN-0.8Cr3C2-8Co cemented carbides possess higher hardness, and better cutting performance than denitriding inserts under the same cutting condition. There were abrasive wear, oxidative wear and obviously abrasive wear on the flank of nitriding inserts. As for denitriding inserts, there were combination of adhesive wear, oxidative wear, built-upedge (BUE) and abrasive wear on the flank face. Their dominant failure mechanism was abrasive wear. In addition, adhesive wear also played a role in the tools failure to some extent for both nitriding and denitriding cemented carbides cutting tools.

PROGRAMMABLE INDUCTANCE AND CAPACITANCE GENERATOR

Hua, Shang Qing,Hua, Wu Li,Sheng, Qu Yan,Jian, Ma Huai 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1

This paper introduces an impedance converter principle of simulated pure inductance and capacitance by using operational amplifiers, in addition, it provides the circuitry configurations according to this principle. The generator can produce equivalent inductance from 0.1H to 6553500H or equivalent capacitance from 10^(-10) F to 65535 × 10^(-7) by programming control. Its circuit is very simple, this generator can be programmed control conveniently and integrated easily. It especially has certain advantages in simulating large inductance or capacitance and programming control.

Application of RBF Neural Network in Fault Diagnosis of FOG SINS

Wu Lei,Sun Rong-Ping,Cheng Jian-Hua 제어로봇시스템학회 2008 제어로봇시스템학회 국제학술대회 논문집 Vol.2008 No.10

Taking FOG SINS (fiber-optic gyroscope strapdown inertial system) as an object, a new fault diagnostic scheme based on RBF(radial basis function) neural network is proposed. Being capable of training and simulating data off-line, neural networks provide a solution to overcome some drawbacks of the quantitative fault diagnosis. The fault tree of FOG SINS is analyzed, which is the basis of the study of neural network fault diagnosis technology. The structure and inferential mechanism of RBF network used for elementary fault diagnosis are discussed in detail. Training simulation results of the neural network are given and an improved effect with real data is obtained, which show the feasibility of the proposed scheme.

Three New 29 Carbon Skeletons Pentacyclic Triterpenoids and S-equol from Biogas Slurry

Jian-Feng Xu,Hui-Bin Wu,Ding-Cai Liu,Long Sha,Wen-Hui Wu,Hua Fan,Yishan Song,Hong-Guang Zhu 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.12

Bioactive natural products were firstly obtained from biogas slurry. Three new 29 carbon skeletons of the pentacyclic triterpenoid compounds 24-norolean-12-ene-3,22-dione (1), 3β-hydroxy-24-norolean-12-ene-22-one (2), 3α-hydroxy-24-norolean-12-ene-22-one (3), as well as one known compound S-equol (4) were isolated and purified from the MeOH extract of chicken manure biogas slurry. The molecular structures of the four compounds were elucidated based on the extensive spectroscopic data analysis, and the structure of Compound 1 was further confirmed by single-crystal X-ray diffraction. The structures of Compounds 1, 2, and 3 are similar with oleanolicum and hederagenin that has excellent anti-tumor activities. The cytotoxicity against five cancer cell lines (Hela, A549, MCF7, PC3, and B16) of Compounds 1–4 was tested. Similar to Compound 4 (S-equol), Compounds 1–3 (pentacyclic triterpenoids) showed cytotoxicity activity against different tumor cell lines. Compounds 1–3 showed slightly lower cytotoxicity activities than Compound 4. The IC50 of Compound 4 was determined to be 9.7–27.6 μM, while the IC50 values of 1–3 were 17.6–65.3 μM. There are no significant differences in the cytotoxicity capacities between Compounds 1, 2, and 3.

OTX1 Contributes to Hepatocellular Carcinoma Progression by Regulation of ERK/MAPK Pathway

Hua Li,Qian Miao,Chun-wei Xu,Jian-hui Huang,Yue-fen Zhou,Mei-juan Wu 대한의학회 2016 Journal of Korean medical science Vol.31 No.8

Orthodenticlehomeobox 1 (OTX1) overexpression had previously been associated with the progression of several tumors. The present study aimed to determine the expression and role of OTX1 in human hepatocellular carcinoma (HCC). The expression level of OTX1 was examined by quantitative real-time PCR (qRT-PCR) in 10 samples of HCC and paired adjacent non-cancerous tissues, and by immunohistochemistry (IHC) analysis in 128 HCC samples and matched controls. The relationship between OTX1 expression and the clinicopathological features werealso analyzed. Furthermore, the effects of OTX1 knockdown on cell proliferation and migration were determined in HCC cell lines. Axenograft mouse model was also established to investigate the role of OTX1 in HCC tumor growth. TheqRT-PCR and IHC analyses revealed that OTX1 was significantly elevated in HCC tissues compared with the paired non-cancerous controls. Expression of OTX1 was positively correlated with nodal metastasis status (P = 0.009) and TNM staging (P = 0.001) in HCC tissues. In addition, knockdown of OTX1 by shRNA significantly inhibited the proliferation and migration, and induced cell cycle arrest in S phase in vitro. Tumor growth was markedly inhibited by OTX1 silencing in the xenograft. Moreover, OTX1 silencing was causable for the decreased phosphorylation level of ERK/MAPK signaling. In conclusion, OTX1 contributes to HCC progression possibly by regulation of ERK/MAPK pathway. OTX1 may be a novel target for molecular therapy towards HCC.

VISUALIZATION ON VORTEX OF CROSS FLOW PAST A CIRCULAR CYLINDER NEAR A WALL BY PIV

JIAN WU,HUANG SHE-HUA,QI E-RONG,WEI LI 한국수자원학회 2005 한국수자원학회 학술대회 Vol.2005 No.1

Synthesis, properties, calculations and applications of small molecular host materials containing oxadiazole units with different nitrogen and oxygen atom orientations for solution-processable blue phosphorescent OLEDs

Hua Ye,Hongyu Wu,Liangyuan Chen,Songhua Ma,Kaifeng Zhou,Guobing Yan,Jiazhong Shen,Dongcheng Chen,Shi‑Jian Su 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.2

A series of new small molecules based on symmetric electron-acceptor of 1,3,4-oxadiazole moiety or its asymmetric isomerof 1,2,4-oxadiazole unit were successfully synthesized and applied to solution-processable blue phosphorescent organic lightemittingdiodes for the first time, and their thermal, photophysical, electrochemical properties and density functional theorycalculations were studied thoroughly. Due to the high triplet energy levels (ET, 2.82–2.85 eV), the energy from phosphorescentemitter of iridium(III) bis[(4,6-difluorophenyl)-pyridinate-N,C2′]picolinate (FIrpic) transfer to the host molecules couldbe effectively suppressed and thus assuring the emission of devices was all from FIrpic. In comparison with the para-modeconjugation in substitution of five-membered 1,3,4-oxadiazole in 134OXD, the meta-linkages of 1,2,4-isomer appending withtwo phenyl rings cause the worse conjugation degree and the electron delocalization as well as the lower electron-withdrawingability for the other 1,2,4-oxadiazole-based materials. Noting that the solution-processed device based on 134OXD containing1,3,4-oxadiazole units without extra vacuum thermal-deposited hole/exciton-blocking layer and electron-transportinglayer showed the highest maximum current efficiency (CEmax) of 8.75 cd/A due to the excellent charge transporting ability of134OXD, which far surpassed the similar devices based on other host materials containing 1,2,4-oxadiazole units. Moreover,the device based on 134OXD presented small efficiency roll-off with current efficiency (CE) of 6.26 cd/A at high brightnessup to 100 cd/m2. This work demonstrates different nitrogen and oxygen atom orientations of the oxadiazole-based hostmaterials produce major impact on the optoelectronic characteristics of the solution-processable devices.

Laparoscopic left hepatectomy in swine: a safe and feasible technique

Hua Zhang,Tao Liu,Yue Wang,Hai-feng Liu,Jian-tao Zhang,Yan-shuang Wu,Lei Lei,Hong-bin Wang 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.3

A purely laparoscopic four-port approach was created forleft hepatectomy in pigs. A polyethylene loop was placed onthe left two hepatic lobes for traction and lift. Next,penetrating ligation of the lobes using of a double row of silksutures was performed to control bleeding. A direct hepatictransection was completed using a monopolar hook electrodewithout meticulous dissection of the left hepatic vein. Theraw surface of the liver was coagulated and sealed with fibringlue. Lobes were retrieved through an enlarged portal. Laparoscopic hepatic lobectomy was completed in all pigswithout the use of specialized instruments and with a meanoperative time of 179 ± 9 min. No significant perioperativecomplications were observed. The average weight of eachresected lobe was 180 ± 51 g. Complete blood count as well asserum organics and enzyme levels normalized after about 2weeks. During necropsy, adhesion of the hepatic raw surfaceto the gastric wall and omentum were observed. No otherabnormalities were identified. This minimally invasive lefthepatectomy technique in swine could serve as a usefulmodel for investigating liver diseases and regeneration, andoffer preclinical information to improve hepatobiliary surgical procedures.