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( Hyeongwon Choi ),( Min Kyung Shin ),( Hye Jin Ahn ),( Youngsook Son ),( Hun Kuk Park ),( Kyung Sook Kim ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2
Background: Chemical agents that can potentially cause skin irritation are normally assessed in vitro by performing cell viability or cytokine expression assays. However, these methods do not always provide decisive translational results and are not sensitive enough to detect toxicity. Objectives: We investigate the mechanical properties of keratinocytes as novel endpoints for safety assessment of chemical agents (sodium lauryl sulphate) using human keratinocytes.Methods: Cell proliferation, membrane integrity, release of cytokines, and cell morphology were observed using biological methods. Changes in stiffness and surface roughness as biomechanical parameters were investigated by atomic force microscopy. Results: Cell viability decreased to 96.26 【 3.87%, 97.78 【 2.16%, 81.18 【 1.19%, and 42.38 【 2.13 at SLS concentrations of 5, 10, 25, and 50 レM, respectively.When the SLS concentration was increased from 0 to 10 レM, stiffness decreased from 0.067 【 0.008 to 0.025 【 0.004 at the center, 0.101 【 0.008 to 0.028 【 0.003 at the periphery, and 0.076 【 0.013 to 0.026 【 0.003 at the junction. Conclusion: Physiological changes were seen at a relatively high dose of SLS (? 25 レM), while the mechanical properties of keratinocytes responded linearly to SLS, even at low doses (÷ 10 レM). These results indicate that the mechanical properties of keratinocytes can be used as sensitive endpoints for in vitro cytotoxic testing of potential chemical irritants.
Choi, Hyeongwon,Shin, Min Kyung,Ahn, Hey Jin,Lee, Tae Ryong,Son, Youngsook,Kim, Kyung Sook John Wiley Sons, Inc. 2018 Microscopy research and technique Vol.81 No.11
<P><B>Abstract</B></P><P>Chemical agents that can potentially cause skin irritation are typically tested in animal models or in vitro assays of cell viability or cytokine expression. However, these methods do not always provide translatable results and are not sufficiently sensitive for subtoxicity detection. Here, we introduce the mechanical properties of keratinocytes as novel endpoints for the safety assessment of chemical agents at the subtoxicity level. Human primary keratinocytes were treated with various concentrations of sodium lauryl sulfate (SLS) and their biological properties (proliferation, membrane integrity, inflammatory response, and morphology) were observed. Their biomechanical and geometrical parameters (stiffness and surface roughness) were also investigated by atomic force microscopy. Keratinocyte morphophysiological changes and inflammatory responses were significant at ≥25 μM SLS. The keratinocytes became less stiff due to changes in the distribution of F‐actin filaments and α‐tubulin; these changes were significant even at lower doses of SLS (≤10 μM). The morphophysiological changes of keratinocytes were clearly seen at a relatively high dose of SLS, while the mechanical properties of keratinocytes responded linearly to SLS at lower doses. Therefore, changes in mechanical properties can be used as new endpoints for in vitro toxicity testing with keratinocytes.</P>
Choi, Hyeongwon,Kim, Dong-jin,Nam, Seungwoo,Lim, Sunki,Hwang, Jae-Sung,Park, Ki Sook,Hong, Hyun Sook,Won, Younsun,Shin, Min Kyung,Chung, Eunkyung,Son, Youngsook Elsevier 2018 JOURNAL OF DERMATOLOGICAL SCIENCE Vol.89 No.3
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Atopic dermatitis (AD) is a chronic inflammatory skin disorder characterized by intense pruritus and eczematous lesion. Substance P (SP) is an 11-amino-acid endogenous neuropeptide that belongs to the tachykinin family and several reports recently have supported the anti-inflammatory and tissue repairing roles of SP.</P> <P><B>Objective</B></P> <P>In this study, we investigated whether SP can improve AD symptoms, especially the impaired skin barrier function, in 2, 4, 6-trinitrochlorobenzene (TNCB)-induced chronic dermatitis of NC/Nga mice or not.</P> <P><B>Method</B></P> <P>AD-like dermatitis was induced in NC/Nga mice by repeated sensitization with TNCB for 5 weeks. The experimental group designations and topical treatments were as follows: vehicle group (AD-VE); SP group (AD-SP); and SP with NK1R antagonist CP99994 (AD-SP-A) group. Histological analysis was performed to evaluate epidermal differentiation, dermal integrity, and epidermal nerve innervation in AD-like lesions. The skin barrier functions and pruritus of NC/Nga mice were evaluated by measuring transepidermal water loss (TEWL) and scratching behavior, respectively.</P> <P><B>Result</B></P> <P>Topical SP treatment resulted in significant down-regulation of Ki67 and the abnormal-type keratins (K) K6, K16, and K17, restoration of filaggrin and claudin-1, marked reduction of TEWL, and restoration of basement membrane and dermal collagen deposition, even under continuous sensitization of low dose TNCB. In addition, SP significantly reduced innervation of itch-evoking nerve fibers, gelatinase activity and nerve growth factor (NGF) expression in the epidermis but upregulated semaphorin-3A (Sema3A) expression in the epidermis, along with reduced scratching behavior in TNCB-treated NC/Nga mice. All of these effects were completely reversed by co-treatment with the NK1R antagonist CP99994. In cultured human keratinocytes, SP treatment reduced expression of TGF-α, but upregulated TGF-β and Sema3A.</P> <P><B>Conclusion</B></P> <P>Topically administered SP can restore normal skin barrier function, reduce epidermal infiltration of itch-evoking nerve fibers in the AD-like skin lesions, and alleviate scratching behavior. Thus, SP may be proposed as a potential medication for chronic dermatitis and AD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Topical Substance P treatment reduced AD-like symptoms in TNCB-induced NC/Nga mice. </LI> <LI> Topical Substance P treatment reduced epidermal acanthosis and expressed normal keratin profiles in AD-like mice. </LI> <LI> Topical Substance P treatment regulated both small nerve attraction and repulsion factors in AD-like skin. </LI> <LI> Topical Substance P treatment recovered skin barrier function and reduced pruritus in AD-like mice. </LI> </UL> </P>
강정훈(JeongHoon Kang),채철승(Chulseoung Chae),강형원(Hyeongwon Kang),김창우(Changwoo Kim),최효섭(HyoSeop Choi) 대한전자공학회 2020 대한전자공학회 학술대회 Vol.2020 No.8
In this paper, we have implemented a framework that simply writes a pre-processing task and a simple driver function without re-defining the task of querying and pre-processing data from the DB and storing it in the DB every time. It consists of a system that divides the preprocessing part and the part stored in the DB and modularizes each task and function. This makes it possible to process lost data at a faster rate. Also, in the multi-processing process, when each process is finished, it is not stopped and the next task can be executed immediately, so that no time is wasted. It also facilitates deployment by configuring the configuration software as a docker container. Also, to distribute the design pattern and framework configuration system, the container structure that builds the execution environment of the application was applied using docker and docker-compose, which are open source platforms.
( Hyung-jin Park ),( Yong-yon Won ),( Tae In Kim ),( Hyeongwon Choi ),( Ki-heon Jeong ),( Ji-youn Sung ),( Min Kyung Shin ),( Mu-hyoung Lee ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.2
Background: Urticarial dermatitis has urticarial and eczematous features with varying predominance at different times. Many clinicians still consider cases as other dermatitis including adult-onset atopic dermatitis due to the overlap occasionally found between them. Objectives: To evaluate the basement membrane status in urticarial dermatitis and compared it with those in normal skin and adult-onset atopic dermatitis. Methods: Basement membrane thickness was evaluated using Periodic-Acid-Schiff (PAS) staining, and immunohistochemical staining of type IV collagen and integrin α6 was performed to study basement membrane, in urticarial dermatitis, adult-onset atopic dermatitis, and normal skin. Results: Urticarial dermatitis and normal groups did not show significant difference in PAS-positive basement membrane thickness (p=0.374) and mean grades of staining intensity for type IV collagen (p=0.539) and integrin α6 in basement membrane (p=0.839). Basement membrane thickness and the mean grades of staining intensity for type IV collagen and integrin α6 in basement membrane of adult-onset atopic dermatitis group were significantly lower than those observed in normal and urticarial dermatitis groups (all, p<0.05). Conclusion: Basement membrane status in urticarial dermatitis was intact compared with normal skin. Histological parameters using special staining can be useful criteria in differentiating urticarial dermatitis from adult-onset atopic dermatitis.