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The Diagnosis and Pharmacologic Management of Arrhythmia
Heon Kil Lim Korean Society of Critical Care Medicine 2002 Acute and Critical Care Vol.17 No.2
Arrhythmias are categorized as due to abnormal impulse formation, abnormal impulse propagation or combined abnormalities of impulse formation and propagation. The primary tools used in the diagnosis of cardiac arrhythmias are the history, physical examination,12-lead electrocardiogram,24-hour continuous electrocardiographic recording, exercise test,intermittent electrocardiographic recording and clinical electrophysiologic study. Optimal management of cardiac arrhythmias requires knowledge of their mechanism,etiology, natural history and effect on the hemodynamic state.And the antiarrhythmic treatment must be monitored closely for its initial and continued effectiveness and for adverse effects.
Polymerase Chain Reaction에 의한 Methicillin-Resistant Staphylococcus mecA유전자의 신속 동정과 클로닝
임헌길(Heon-Kil Lim),강현(Hyun Kang),한유진(You-Jin Han),김수영(Soo-Young Kim),강영숙(Young-Sook Kang),육동인(Dong-In Yuk),이경원(Kyung-Won Lee),정윤섭(Yun-Sop Chong),박기호(Ki-Ho Park),김의종(Eui-Chong Kim),이형환(Hyung-Hoan Lee) 大韓微生物學會 1997 大韓微生物學會誌 Vol.32 No.2
林龍進,崔興辰,尹南植,朴憲吉 慶北大學校 1981 論文集 Vol.31 No.-
Concentrations of carbon monoxide and nitrogen oxides in the exhaust gas of gasoline engine with a water droplet system were determined quantitatively and compared with those of the normal engine. The concentration of nitrogen oxides was increased with the engine speed, but that of carbon monoxide was decreased. And all of these gases' formtaion were dropped remarkably in the water supplied engine compared with the normal one. This result seems to indicate that the negative enthalpy change of decomposition of water decreases the maximum temperature in the combustion chamber partially, and the formation of water gas contributes to reducing the amount of carbon monoxide by trapping unreacted carbon particles.
Kim, Kil Hyun,Lim, Seungmo,Kang, Yang Jae,Yoon, Min Young,Nam, Moon,Jun, Tae Hwan,Seo, Min-Jung,Baek, Seong-Bum,Lee, Jeom-Ho,Moon, Jung-Kyung,Lee, Suk-Ha,Lee, Su-Heon,Lim, Hyoun-Sub,Moon, Jae Sun,Park The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.2
Virus-induced gene silencing (VIGS) is an effective tool for the study of soybean gene function. Successful VIGS depends on the interaction between virus spread and plant growth, which can be influenced by environmental conditions. Recently, we developed a new VIGS system derived from the Soybean yellow common mosaic virus (SYCMV). Here, we investigated several environmental and developmental factors to improve the efficiency of a SYCMV-based VIGS system to optimize the functional analysis of the soybean. Following SYCMV: Glycine max-phytoene desaturase (GmPDS) infiltration, we investigated the effect of photoperiod, inoculation time, concentration of Agrobacterium inoculm, and growth temperature on VIGS efficiency. In addition, the relative expression of GmPDS between non-silenced and silenced plants was measured by qRT-PCR. We found that gene silencing efficiency was highest at a photoperiod of 16/8 h (light/dark) at a growth temperature of approximately $27^{\circ}C$ following syringe infiltration to unrolled unifoliolate leaves in cotyledon stage with a final SYCMV:GmPDS optimal density $(OD)_{600}$ of 2.0. Using this optimized protocol, we achieved high efficiency of GmPDS-silencing in various soybean germplasms including cultivated and wild soybeans. We also confirmed that VIGS occurred in the entire plant, including the root, stem, leaves, and flowers, and could transmit GmPDS to other soybean germplasms via mechanical inoculation. This optimized protocol using a SYCMV-based VIGS system in the soybean should provide a fast and effective method to elucidate gene functions and for use in large-scale screening experiments.