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Rolled Fingerprint Construction Using MRF-Based Nonrigid Image Registration
Dongjin Kwon,Il Dong Yun,Lee, S U IEEE 2010 IEEE TRANSACTIONS ON IMAGE PROCESSING - Vol.19 No.12
<P>This paper proposes a new rolled fingerprint construction approach incorporating a state-of-the-art nonrigid image registration method based upon a Markov random field (MRF) energy model. The proposed method finds dense correspondences between images from a rolled fingerprint sequence and warps the entire fingerprint area to synthesize a rolled fingerprint. This method can generate conceptually more accurate rolled fingerprints by preserving the geometric properties of the finger surface as opposed to ink-based rolled impressions and other existing rolled fingerprint construction methods. To verify the accuracy of the proposed method, various comparative experiments were designed to reveal differences among the rolled construction methods. The results show that the proposed method is significantly superior in various aspects compared to previous approaches.</P>
Dongjin Shin,Soo-Kwon Park,Un-Ha Hwang,Jong-Hee Lee,Sang-Ik Han,Min-Hee Nam,Dong-Soo Park 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Understanding salt tolerance mechanisms is important for the increase of crop yields, and so, several screening approaches were developed to identify plant genes which are involved in salt tolerance of plants. Here, we transformed the Arabidopsis cDNA library into a salt-sensitive calcineurin (CaN)-deficient (cnbD) yeast mutant and isolated the colonies which can suppress salt-sensitive phenotype of cnbD mutant. Through this functional complementation screen, a total of 34 colonies functionally suppressed the salt-sensitive phenotype of cnbD yeast cells, and sequencing analysis revealed that these are 9 genes, including CaS, AtSUMO1 and AtHB-12. Among these genes, the ectopic expression of CaS gene increased salt tolerance in yeast, and CaS transcript was up-regulated under high salinity conditions. CaS-antisense transgenic plants showed reduced root elongation under 100 mM NaCl treatment compared to the wild type plant, which survived under 150 mM NaCl treatment, whereas CaS-antisense transgenic plant leaves turned yellow under 150 mM NaCl treatment. These results indicate that the expression of CaS gene is important for stress tolerance in yeast and plants.
Dongjin Shin,Soo-Kwon Park,Un-Ha Hwang,Dong-Soo Park 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Glycolysis is responsible for the conversion of glucose into pyruvate and for supplying reducing power and several metabolites. Fructose-1,6-bisphosphate aldolase (AtFBA1), a central enzyme in the glycolysis pathway, was isolated by functional complementation of the salt-sensitive phenotype of a calcineurin (CaN)-deficient yeast mutant. Under high salinity conditions, aldolase activity and the concentration of NADH were compromised. However, expression of AtFBA1 maintained aldolase activity and the NADH level in yeast cells. AtFBA1 shares a high degree of sequence identity with known class I type aldolases, and its expression was negatively regulated by stress conditions including NaCl. The fusion protein GFP-AtFBA1 was localized in the cytosol of Arabidopsis protoplasts. The seed germination and root elongation of AtFBA1 knock-out plants exhibited sensitivity to ABA and salt stress. These results indicate that AtFBA1 expression and aldolase activity is important for stress tolerance in yeast and plants.
Development of marker-free transgenic rice expressing wheat storage protein, Glu-Dx5
Soo-Kwon Park,Woon-Ha Hwang,DongJin Shin,Ji-Yoon Lee,Jun-Hyeun Cho,Myung-Hee Kim,Min-Hee Nam,Dong-Soo Park 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Development of transgenic plant increasing crop yield or disease resistance is good way to solve the world food shortage. However, the persistence of marker genes in crops leads to serious public concerns about the safety of transgenic crops. In the present study, we developed marker-free transgenic rice inserted high molecular-weight glutenin subunit (HMW-GS) gene (Dx5) from the Korean wheat cultivar ‘Jokyeong’ using Agrobacterium-mediated co-transformation method. The Dx5’s own promoter was used for protein expression. Two expression cassettes comprised of separate DNA fragments containing only the Dx5 and hygromycin resistance (HPTII) genes were introduced separately into Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Dx5 or HPTII was infected into rice calli at a 3: 1 ratio of EHA105 with Dx5 gene and EHA105 with HPTII gene expressing cassette. Then, among 270 hygromycin-resistant transformants, we obtained 27 transgenic lines inserted with both the Dx5 and HPTII genes into the rice genome. We reconfirmed integration of the Dx5 gene into the rice genome by Southern blot analysis. Wheat Dx5 transcripts in T1 rice seeds were examined with semi-quantitative RT-PCR. Protein expression of the Dx5 was analyzed with Western blot using polyclonal antibody recognising x-type of glutenin subunits in T1 seeds. It was suggested that the protein-processing system was conserved between rice and wheat. Finally, the marker-free plants containing only the Dx5 gene were successfully screened at the T1 generation.
Soo-Kwon Park(박수권),Tackmin Kwon(권택민),Jong-Hee Lee(이종희),DongJin Shin(신동진),Woon-Ha Hwang(황운하),You-Chun Song(송유천),Jun-Hyun Cho(조준현),Min-Hee Nam(남민희),Seung-Ho Jeon(전승호),Sang-Yeol Lee(이상열),Dong-Soo Park(박동 한국생명과학회 2012 생명과학회지 Vol.22 No.9
작물의 수확량이나 병 저항성을 증가시키는 형질전환 식물체 개발은 세계 식량 부족을 해결하는 좋은 방법이다. 하지만 항생제나 제초제의 사용은 형질전환 작물의 안전에 대해서 일반 사람들의 심각한 우려를 초래한다. 본 연구에서는, 아그로박테리움을 이용한 동시 형질전환 방법을 이용하여 한국의 밀 재배종인 ‘조경밀’의 유전자인, 고분자 글루테닌 서브유닛[high molecular-weight glutenin subunit (HMW-GS)] Dx5가 삽입된 마커프리 형질전환벼를 개발하였다. 각각 Dx5 유전자와 하이그로마이신(HPTII) 저항성 유전자만으로 구성된 두 종류의 발현 카셋트(Two expression cassettes)를 독립적으로 아그로박테리움 EHA105에 도입하였고, Dx5와 HPTII가 도입된 각각의 EHA105 아그로박테리움을 3:1 비율로 혼합하여 벼 캘러스에 접종하였다. 66개의 HPTII 저항성 형질전환체 중에서 벼 게놈에 Dx5와 HPTII가 모두 삽입된 2개의 형질전환 라인을 획득하였다. Dx5와 HPTII가 벼 게놈에 도입된 것을 Southern blot을 통해서 다시 확인하였다. 또한, semi-quantitative RT-PCR을 통해 형질전환벼 T1 세대 종자의 밀 Dx5 전사여부를 확인하였고 결국, Dx5 유전자만을 가지는 마커프리 형질전환벼를 T1 세대에서 선발할 수 있었다. 본 연구 결과는 두 종류의 발현 카셋트를 사용한 아그로박테리움 동시 접종 시스템이 마커프리 형질전환벼를 생산하기 위한 효과적인 전략이 될 수 있음을 보여준다. Development of transgenic plant increasing crop yield or disease resistance is good way to solve the world food shortage. However, the persistence of marker genes in crops leads to serious public concerns about the safety of transgenic crops. In the present paper, we developed marker-free transgenic rice inserted high molecular-weight glutenin subunit (HMW-GS) gene (Dx5) from the Korean wheat cultivar ‘Jokyeong’ using Agrobacterium-mediated co-transformation method. Two expression cassettes comprised of separate DNA fragments containing only the Dx5 and hygromycin resistance (HPTII) genes were introduced separately into Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Dx5 or HPTII was infected into rice calli at a 3: 1 ratio of EHA105 with Dx5 gene and EHA105 with HPTII gene expressing cassette. Then, among 66 hygromycin-resistant transformants, we obtained two transgenic lines inserted with both the Dx5 and HPTII genes into the rice genome. We reconfirmed integration of the Dx5 and HPTII genes into the rice genome by Southern blot analysis. Wheat Dx5 transcripts in T1 rice seeds were examined with semi-quantitative RT-PCR. Finally, the marker-free plants containing only the Dx5 gene were successfully screened at the T1 generation. These results show that a co-infection system with two expression cassettes could be an efficient strategy to generate marker-free transgenic rice plants.
The effective method to screen high Fe content brown rice
Woon-Ha Hwang,Soo-Kwon Park,Dongjin Shin,Min-Hee Nam,In-Jung Lee,Dong-Soo Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Even rice is one of the most important food crops in the world, its micronutrient contents including iron is not enough to solve mineral malnutrition which is a significant public health issue in most developing countries. Iron deficiency is probably the most widespread micronutrient deficiency in humans. Experts estimate that a rice based diet should contain 14.5 ppm iron in endosperm. However, Cesar P et al reported that average iron content in milled rice was 2 ~ 3 ppm, whease it was 10 ~ 11 ppm in brown rice. Fe content of rice is usually measured by inductively plasma spectrometry (ICP). It takes times and could make error while sample processing. To breed high iron contained rice variety, the effective screen method for select high iron contained elite line is essential. To develop more effective method in screening high Fe contained brown rice, we investigate the relation the leaf chlorophyll content with iron content in brown rice. Result of analyzing leaf chlorophyll content of OsNAS3-OX which contain more Fe than wild-type plant after cultivated on Fe limited MS medium, those of OsNAS3-OX was higher than those of wild-type plant in 0 and 20 % Fe contained MS medium. After measured Fe content in twenty kinds of brown rice, we cultivated those in Fe limited MS medium then investigate the relation of leaf chlorophyll content with Fe content of brown rice. In 0 and 5 % Fe contained MS medium, the leaf chlorophyll content was highly related with Fe content of brown rice as 0.66 and 0.79. Though these result, analyze of leaf chlorophyll content cultivated in 5 % Fe content in MS media was effect on screening high Fe contained.
A large-scale screening analysis for the evaluation of Bakanae disease in rice
Myung-Hee Kim,Saet-Byeol Lee,Tackmin Kwon,Un-Ha Hwang,Soo-Kwon Park,Yeong-Nam Youn,Jong-Hee Lee,Jun-Hyun Cho,Dongjin Shin,Sang-Ik Han,Un-Sang Yeo,You-Chun Song,Min-Hee Nam,Dong-Soo Park 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Bakanae disease of rice, caused by Fusarium moniliforme Sheldon, the imperfect stage of Gibberella fujikuroi, is one of the most important rice diseases worldwide, but no rice variety has been found to be completely resistant to this fungus. Cultivation of resistant cultivars is the most beneficial way of reducing quantitative or qualitative losses to for bakanae disease in rice. To facilitate the study of this disease, accurate and large scale screening methods were developed for the inoculation and evaluation of Bakanae disease. Even and large scale infection was achieved by using F. moniliforme spore in tissue embedding cassette and seedling tray. The efficiency of F. moniliforme infection with the concentration of 1×106 spore/ml caused better distribution (F-value=33.96) than 1×102 (F-value=10.69), and 1×104 spore/ml (F-value=2.63). We established new criteria of healthy and non-healthy plant, and introduced calculation of proportion of healthy plants to meet fast evaluation of resistance level of each variety. The effect of F. moniliforme strains containing different genetic background was also evaluated with rice varieties to figure out the stability of resistance level. GA3 response of rice variety was significantly correlated with bakanae disease, but it did not adequate for direct indicator of bakanae disease resistance. These results indicated that a large scale infection method developed in this study is fast and reproducible, as well as a disease evaluation system provides an accurate measurement of bakanae disease resistance of rice.