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Kim, Chung-Hui,Cuong, Dang-Van,Kim, Jong-Su,Kim, Na-Ri,Kim, Eui-Yong,Han, Jin The Korean Society of Pharmacology 2003 The Korean Journal of Physiology & Pharmacology Vol.7 No.1
Recent studies indicated that cancer cells become resistant to ionizing radiation (IR) and chemotherapy drugs by enhanced DNA repair of the lesions. Therefore, it is expected to increase the killing of cancer cells and reduce drug resistance by inhibiting DNA repair pathways that tumor cells rely on to escape chemotherapy. There are a number of key human DNA repair pathways which depend on multimeric polypeptide activities. For example, Ku heterodimer regulatory DNA binding subunits (Ku70/Ku80) on binding to double strand DNA breaks (DSBs) are able to interact with 470-kDa DNA-dependent protein kinase catalytic subunit (DNA-PKcs), and are essential for DNA-dependent protein kinase (DNA-PK) activity. It has been known that DNA-PK is an important factor for DNA repair and also is a sensor-transmitting damage signal to downstream targets, leading to cell cycles arrest. Our ultimate goal is to develop a treatment of breast tumors by targeting proteins involved in damage-signaling pathway and/or DNA repair. This would greatly facilitate tumor cell cytotoxic activity and programmed cell death through DNA damaging drug treatment. Therefore, we designed a domain of Ku80 mutants that binds to Ku70 but not DNA end binding activity and used the peptide in co-therapy strategy to see whether the targeted inhibition of DNA-PK activity sensitized breast cancer cells to irradiation or chemotherapy drug. We observed that the synthesized peptide (HNI-38) prevented DNA-PKcs from binding to Ku70/Ku80, thus resulting in inactivation of DNA-PK activity. Consequently, the peptide treated cells exhibited poor to no DNA repair, and became highly sensitive to IR or chemotherapy drugs, and the growth of breast cancer cells was inhibited. Additionally, the results obtained in the present study also support the physiological role of resistance of cancer cells to IR or chemotherapy.
Detection of Mitochondrial ATP-Sensitive Potassium Channels in Rat Cardiomyocytes
Dang Van Cuong,Nari Kim,Euiyong Kim,Youngsuk Lee,Hyunju Kim,Sunghyun Kang,Dae Young Hur,Hyun Joo,Young Shik Park,Yong Geun Hong,Sangkyung Lee,Joonyong Chung,Daehyun Seog,Jin Han 대한생리학회-대한약리학회 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.4
Mitochondrial ATP-sensitive potassium (mitoK<SUB>ATP</SUB>) channels play a role in early and late ischemic preconditioning. Nevertheless, the subunit composition of mitoK<SUB>ATP</SUB> channels remains unclear. In this study, we investigated the subunit composition of mitoK<SUB>ATP</SUB> channels in mitochondria isolated from rat cardiac<FONT COLOR=RED><SPAN STYLE= font-size:11pt; > </SPAN>myocytes. Mitochondria were visualized using the red fluorescence probe, Mitrotracker Red, while mitoK<SUB>ATP</SUB> channels were visualized using the green fluorescence probe, glibenclamide-BODIPY. The immunofluorescence confocal microscopy revealed the presence of Kir6.1, Kir6.2 and SUR2 present in the cardiac mitochondria. Western blot analysis was carried to further investigate the nature of mitoK<SUB>ATP</SUB> channels. For SUR proteins, a 140-kDa immunoreactive band that corresponded to SUR2, but no SUR1 was detected. For Kir6.2, three bands (∼44, ∼46, and ∼30 kDa) were detected, and a specific ∼46-kDa immunoreactive band corresponding to Kir6.1 was also observed. These observations suggest that the subunits of mitoK<SUB>ATP</SUB> channels in rat myocytes include Kir6.1, Kir6.2, and a<FONT COLOR=RED><SPAN STYLE= font-size:11pt; > </SPAN>SUR2-related sulfonylurea-binding protein.
Deep neural network-based prediction for low-energy beam transport tuning
Kim Dong-Hwan,Kim Han-Sung,Kwon Hyeok-Jung,Lee Seung-Hyun,Yun Sang-Pil,Kim Seung-Geun,Yu Yong-Gyun,Dang Jeong-Jeung 한국물리학회 2023 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.83 No.8
Time-varying characteristics of an ion source are induced by environmental change or aging of parts inevitably, making a data-driven prediction model inaccurate. We consider non-invasively measured beam profles as important features to represent initial beam from ion sources in real time. Beam-induced fuorescence monitor was tested to confrm change of beam properties by ion source operating conditions during a beam commissioning phase. Machine learning-based regression models were built with beam dynamics simulation datasets over a range of input parameters in the RFQ-based accelerator. Best prediction for the low-energy beam tuning was obtained by deep neural networks model. The methodology presented in the study can help develop advanced beam tuning models with non-invasive beam diagnostics in time-varying systems.
Detection of Mitochondrial ATP-Sensitive Potassium Channels in Rat Cardiomyocytes
Cuong, Dang Van,Kim, Na-Ri,Kim, Eui-Yong,Lee, Young-Suk,Kim, Hyun-Ju,Kang, Sung-Hyun,Hur, Dae-Young,Joo, Hyun,Park, Young-Shik,Hong, Yong-Geun,Lee, Sang-Kyung,Chung, Joon-Yong,Seog, Dae-Hyun,Han, Jin The Korean Society of Pharmacology 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.4
Mitochondrial ATP-sensitive potassium $(mitoK_{ATP})$ channels play a role in early and late ischemic preconditioning. Nevertheless, the subunit composition of $mitoK_{ATP}$ channels remains unclear. In this study, we investigated the subunit composition of $mitoK_{ATP}$ channels in mitochondria isolated from rat cardiac myocytes. Mitochondria were visualized using the red fluorescence probe, Mitrotracker Red, while $mitoK_{ATP}$ channels were visualized using the green fluorescence probe, glibenclamide-BODIPY. The immunofluorescence confocal microscopy revealed the presence of Kir6.1, Kir6.2 and SUR2 present in the cardiac mitochondria. Western blot analysis was carried to further investigate the nature of $mitoK_{ATP}$ channels. For SUR proteins, a 140-kDa immunoreactive band that corresponded to SUR2, but no SUR1 was detected. For Kir6.2, three bands $({\sim}44,\;{\sim}46,\;and\;{\sim}30\;kDa)$ were detected, and a specific ${\sim}46-kDa$ immunoreactive band corresponding to Kir6.1 was also observed. These observations suggest that the subunits of $mitoK_{ATP}$ channels in rat myocytes include Kir6.1, Kir6.2, and a SUR2-related sulfonylurea-binding protein.
Korean Text to Gloss: Self-Supervised Learning approach
Thanh-Vu Dang(Thanh-Vu Dang),김진영(JinYoung Kim),유광현(Gwang-Hyun Yu),김지용(Ji Yong Kim),박영환(Young Hwan Park),이칠우(ChilWoo Lee) 한국스마트미디어학회 2023 스마트미디어저널 Vol.12 No.1
Natural Language Processing (NLP) has grown tremendously in recent years. Typically, bilingual, and multilingual translation models have been deployed widely in machine translation and gained vast attention from the research community. On the contrary, few studies have focused on translating between spoken and sign languages, especially non-English languages. Prior works on Sign Language Translation (SLT) have shown that a mid-level sign gloss representation enhances translation performance. Therefore, this study presents a new large-scale Korean sign language dataset, the Museum-Commentary Korean Sign Gloss (MCKSG) dataset, including 3828 pairs of Korean sentences and their corresponding sign glosses used in Museum-Commentary contexts. In addition, we propose a translation framework based on self-supervised learning, where the pretext task is a text-to-text from a Korean sentence to its back-translation versions, then the pre-trained network will be fine-tuned on the MCKSG dataset. Using self-supervised learning help to overcome the drawback of a shortage of sign language data. Through experimental results, our proposed model outperforms a baseline BERT model by 6.22%.
Changes of Cytosolic $Ca^{2+}$ under Metabolic Inhibition in Isolated Rat Ventricular Myocytes
Kang, Sung-Hyun,Kim, Na-Ri,Joo, Hyun,Youm, Jae-Boum,Park, Won-Sun,Warda, Mohamed,Kim, Hyung-Kyu,Von Cuong, Dang,Kim, Tae-Ho,Kim, Eui-Yong,Han, Jin The Korean Society of Pharmacology 2005 The Korean Journal of Physiology & Pharmacology Vol.9 No.5
To characterize cytosolic $Ca^{2+}$ fluctuations under metabolic inhibition, rat ventricular myocytes were exposed to $200{\mu}M$ 2,4-dinitrophenol (DNP), and mitochondrial $Ca^{2+}$, mitochondrial membrane potential (${\Delta}{\Psi}m$), and cytosolic $Ca^{2+}$ were measured, using Rhod-2 AM, TMRE, and Fluo-4 AM fluorescent dyes, respectively, by Laser Scanning Confocal Microscopy (LSCM). Furthermore, the role of sarcolemmal $Na^+$/$Ca^{2+}$ exchange (NCX) in cytosolic $Ca^{2+}$ efflux was studied in KB-R7943 and $Na^+$-free normal Tyrode's solution (143 mM LiCl ). When DNP was applied to cells loaded with Fluo-4 AM, Fluo-4 AM fluorescence intensity initially increased by $70{\pm}10$% within $70{\pm}10$ s, and later by $400{\pm}200$% at $850{\pm}45$ s. Fluorescence intensity of both Rhod-2 AM and TMRE were initially decreased by DNP, coincident with the initial increase of Fluo-4 AM fluorescence intensity. When sarcoplasmic reticulum (SR) $Ca^{2+}$ was depleted by $1{\mu}M thapsigargin plus $10{\mu}M ryanodine, the initial increase of Fluo-4 AM fluorescence intensity was unaffected, however, the subsequent progressive increase was abolished. KB-R7943 delayed both the first and the second phases of cytosolic $Ca^{2+}$ overload, while $Na^+$-free solution accelerated the second. The above results suggest that: 1) the initial rise in cytosolic $Ca^{2+}$ under DNP results from mitochondrial depolarization; 2) the secondary increase is caused by progressive $Ca^{2+}$ release from SR; 3) NCX plays an important role in transient cytosolic $Ca^{2+}$ shifts under metabolic inhibition with DNP.
Lee, Young-Suk,Kim, Na-Ri,Kim, Hyun-Ju,Joo, Hyun,Kim, Young-Nam,Jeong, Dae-Hoon,Cuong, Dang Van,Kim, Eui-Yong,Hur, Dae-Young,Park, Young-Shik,Hong, Yong-Geun,Lee, Sang-Kyung,Chung, Joon-Yong,Seog, Dae The Korean Society of Pharmacology 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.4
The purpose of the present study was to evaluate the expression of cardiac marker protein in rabbit cardiac tissue that was exposed to ischemic preconditioning (IPC), or ischemiareperfusion injury (IR) using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). We compared 2DE gels of control (uninjured) cardiac tissue with those of IPC and IR cardiac tissue. Expression of one protein was detected in IR heart tissue, however the protein was not detected in the samples of control and IPC tissue. To further characterize the detected protein molecule, the protein in the 2D gel was isolated and subjected to trypsin digestion, followed by MALDI-MS. The protein was identified as myoglobin, which was confirmed also by Western blot analysis. These results are consistent with previous studies of cardiac markers in ischemic hearts, indicating myoglobin as a suitable marker of myocardial injury. In addition, the present use of multiple techniques indicates that proteomic analysis is an appropriate means to identify cardiac markers in studies of IPC and IR.
Youngsuk Lee,Nari Kim,Hyunju Kim,Hyun Joo,Youngnam Kim,Daehoon Jeong,Dang Van Cuong,Euiyong Kim,Dae Young Hur,Young Shik Park,Yong Geun Hong,Sangkyung Lee,Joonyong Chung,Daehyun Seog,Jin Han 대한생리학회-대한약리학회 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.4
The purpose of the present study was to evaluate the expression of cardiac marker protein in rabbit cardiac tissue that was exposed to ischemic preconditioning (IPC), or ischemiareperfusion injury (IR) using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). We compared 2DE gels of control (uninjured) cardiac tissue with those of IPC and IR cardiac tissue. Expression of one protein was detected in IR heart tissue, however the protein was not detected in the samples of control and IPC tissue. To further characterize the detected protein molecule, the protein in the 2D gel was isolated and subjected to trypsin digestion, followed by MALDI-MS. The protein was identified as myoglobin, which was confirmed also by Western blot analysis. These results are consistent with previous studies of cardiac markers in ischemic hearts, indicating myoglobin as a suitable marker of myocardial injury. In addition, the present use of multiple techniques indicates that proteomic analysis is an appropriate means to identify cardiac markers in studies of IPC and IR.