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가축의 장내용물에서 Listeria 속균의 분포도 조사
정병열,임현숙,김봉환 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1
We surveyed the prevalence of Listeria spp. in cecal contents of pig and chicken collected from abattoirs. only 4 L. lnnocua isolates were detected in a total of 100 pig samples. From the 100 chicken samples, 47 were confirmed positive for Listeria spp. Twenty eight of these 47 positive samples contained only L. lnnocua; 15 contained both L. monocytogenes and L. lnnocua; 4 contained only L. mnocytogenes. No other Listeria spp. were detected in this study. Listeria spp. were detected in 471115 (40.9%) blackened Fraser broth samples and in 4/85 (4.7%) samples in which Fraser broth did not blacken. Fourteen (73.7%) L. monocytogenes isolates were recovered after 48 h, not 24 h, Fraser broth secondary enrichment.
경북지역 가축에서 분리한 Salmonella 속균의 혈청형 분포 및 약제 감수성
김규태,정병열,김봉환 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1
The present study was conducted to investigate the prevalence of serotypes and antimicrobial susceptibility of Salmonella isolated from various domestic animals in Gyeongbuk Province during the period from January to December 2000. From the 3,141 animal samples, 51 (1.6%) Salmonella spp. were isolated and belonged to 8 serotypes. Serotypes with decreasing order of prevalence were S. enteritidis (17 isolates; 29.6%), S. agona (11; 20.3%), S. rissen (8; 14.8%), S. gallinarum (4; 7.4%), S. derby (3; 5.5%), S. tvphirmvium (2; 3.7%), S. travis (1; 1.8%), S. montevideo (1; 1.8%) and untypable (4; 12.9%). Antimicrobial susceptibility tests showed that most isolates were susceptible to amikacin (loo%), ampicillin (92.2%), cephalothin (90.2%), chloramphenicol (96.1%), ciprofloxacin (92.2%), genta- micin (92.2%), kanamycin (98.0%), norfloxacin (92.2%), bimethoprim-sulfamethoxazole (100%) and tobramycin (100%). However, antimicrobials to which the high percentage of isolates were resistant were sbeptomycin (37.2%) and tetracycline (47.1%).
Byeong Yeal Jung(정병열),Bum Soo Park(박범수),Ha-Young Kim(김하영),Jae Won Byun(변재원),Aeran Kim(김애란),Albert Byungyun Jeon(전병윤),In Cheul Kim(김인철),Ki Hwa Chung(정기화) 한국생명과학회 2012 생명과학회지 Vol.22 No.8
돼지 정액의 세균오염은 정자활력 감소나 수태율 저하 등을 유발하는데, 특히 Stenotrophomonas maltophilia는 자연환경에 널리 존재하여 비위생적으로 채취된 정액에 많이 오염될 뿐 아니라 사람에게도 병원성을 일으킨다. 본 연구에서는 S. maltophilia의 검사 시간 단축과 정확도를 높이고자 PCR 기법을 개발하였으며, 돼지 원정액에서 이들 세균의 오염율을 조사하고 유효 항생제를 선발하고자 하였다. 돼지 원정액에서 분리 빈도가 높은 18 균종을 대상으로 PCR을 적용한 결과, S. maltophilia에서만 445 bp의 특이 유전자 증폭산물이 확인되었다. 또한 순수 배양된 S. maltophilia는 1.5×10³ CFU/ml까지, 원정액에 혼합된 S. maltophilia에 대해서는 1.5×10⁴ CFU/ml까지 검출이 가능하였다. 2009년에 116건과 2011년 324건 등 총 440건의 원정액 중 26건(5.9%)에서 S. maltophilia가 분리되었으며, 여름과 가을철에 오염율이 상대적으로 높게 나타났다. 균분리법과 PCR간의 일치율은 98.9%, kappa value는 0.906이었으며, PCR의 민감도와 특이도는 각각 100%, 98.7%로 나타났다. 분리균주의 항생제 감수성 시험 결과, enrofloxacin (100%)과 florfenicol (92.3%)에 높은 감수성을 보였으나 amoxicillin/clavulanic acid, apramycin, ceftiofur, penicillin, spectinomycin에는 내성율이 높게 나타났다. 결론적으로 개발된 PCR기법은 민감도, 특이도, 일치율이 높아 균분리법을 보조하고 신속?정확하게 정액 내 오염세균을 확인할 수 있어 효율적인 정액관리가 가능할 것으로 기대된다. Bacteria are frequently contaminated during the collection and processing procedures of boar semen. Of the contaminants, Stenotrophomonas (S.) maltophilia is a Gram-negative bacterium that is widely distributed in a variety of habitats. Although PCR assays have been developed for the detection of S. maltophilia, they cross-react with some species of Xanthomonas. In this study, we designed a primer set for the detection of S. maltophilia in order to target the chiA (GenBank accession no. NC_010943) gene. The specific PCR products were amplified from S. maltophilia only, not from other tested strains that are frequently found in semen. The detection limit of the PCR was 1.5×10³ CFU/ml with pure-cultured S. maltophilia and 1.5×10⁴ CFU/ml with S. maltophilia spiked in semen. Twenty-six (5.9%) S. maltophilia were isolated from 440 semen samples. The PCR results exhibited 98.9% agreement with a comparison of S. maltophilia isolation. Also, the sensitivity and specificity of the PCR were 100% and 98.7%, respectively. In the antimicrobial susceptibility test, S. maltophilia isolates were highly susceptible to enrofloxacin and florfenicol, while the majority of them were resistant to amoxicillin/clavulanic acid, apramycin, ceftiofur, penicillin, and spectinomycin. These results indicated that the PCR using the chiA gene was proven to be reliable and effective for the detection of S. maltophilia with high levels of sensitivity and specificity.
Jung, Byeong-yeal,Jung, Suk-chan,Cho, Dong-hee,Kim, Jong-yeom,Kim, Bong-hwan The Korean Society of Veterinary Science 1999 大韓獸醫學會誌 Vol.39 No.2
Twenty three strains of Escherichia (E) coli O157 : H7 isolated from Korea, Japan, USA were analyzed by pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA and multiplex polymerase chain reaction. Various PFGE patterns of E. coli O157 : H7 were found on the same farm. Most of the E, coli O157 : H7 strains had shiga-like toxin (slt) II gene only (43.5%) or both slt I and slt II genes(30.4%). eaeA gene was highly conserved in the E. coli O157 : H7. There was no correlation between PFGE and slt gene patterns. The results indicate that various genotypes of E. coli O157 : H7 have spread throughout the country and genomic DNA patterns generated by PFGE are highly specific for different strains and have significant value in epidemiologic investigations of infectious disease outbreaks.
Modified sorbitol MacConkey agar for the rapid isolation of Escherichia coli O157:H7
Jung, Byeong-yeal,Jung, Suk-chan,Lee, Na-kyung,Cho, Seong-kun,Cho, Dong-hee,Her, Moon,Yoon, Yong-dhuk,Kim, Bong-hwan The Korean Society of Veterinary Science 1999 大韓獸醫學會誌 Vol.39 No.4
Unlike most Escherichia coli strains, E coli O157 : H7 didn't ferment sorbitol within 24h of incubation and showed a negative reaction for $\beta$-glucuronidase. We developed a new medium for the rapid isolation of E coli O157 : H7 using sorbitol MacConkey agar with cefixime, potassium tellurite and 4-methylumbelliferyl-${\beta}$-D-glucuronide (MUG) as a primary plating medium. The addition of $20{\mu}g/ml$ of vancomycin in enrichment broth for E coli O157 : H7 inhibited lots of Gram positive bacteria. Three strains (10.3%) of 29 non-O157 E coli strains and 3 strains (8.3%) of 36 Salmonella spp were inhibited at the $0.05{\mu}g/ml$ of cefixime and 23 strains (79.3%) of 29 non-O157 E coli strains and 12 strains (33.3%) of 36 Salmonella spp were inhibited at the $2.0{\mu}g/ml$ of potassium tellurite. But none of the E coli O157 : H7 was affected at these concentration. The addition of MUG at $100{\mu}g/ml$ level to sorbitol MacConkey agar with cefixime and potassium tellurite (CTM-SMAC) aided in the rapid isolation of E coli O157 : H7 from samples by checking sorbitol-negative and $\beta$-glucuronidase negative phenotypes simultaneously. In conclusion, inoculation of a positive in the O157 screening test from enrichment broth on CTM-SMAC appeared to be a rapid, cost-effective and sensitive method for the isolation of E coli O157 : H7.
Seroprevalence of <i>Leptospira</i> spp. in Clinically Healthy Racing Horses in Korea
JUNG, Byeong Yeal,LEE, Kyung Woo,HA, Tae Young Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.2
<P>Leptospirosis is a zoonotic disease of global importance, and has a worldwide distribution. The present study aimed to determine leptospiral seroprevalence in clinically healthy racing horses from all three racecourses in Korea. Serum samples from 1,226 racing horses were examined using a microscopic agglutination test to detect the presence of antibodies against 18 <I>Leptospira</I> serovars. Of the tested samples, 307 (25.0%) were found to be positive. The distribution of seroprevalence differed significantly by racecourse (<I>P</I>=0.004); the Jeju course had the highest incidence (31.1%), followed by the Seoul (25.2%) and Busan (19.5%) racecourses. Seasonal variation in seropositivity was also apparent (<I>P</I>=0.000), being lower in spring (13.0%) and winter (12.5%), and higher in summer (36.7%) and autumn (34.7%). No significant age- or gender-related difference in seroprevalence was noted in this study (<I>P</I>>0.05). Seroprevalence was higher (<I>P</I>=0.006) among ponies than among thoroughbreds. Sejroe was the most frequently detected serovar (n=236), followed by Bratislava (n=35), Ballum (n=16), Autumnalis (n=10), and Canicola (n=10). The majority of serum titers were relatively low; most values ranged from 1:100 (n=217) to 1:200 (n=69). These results suggest that the Sejroe serovar may be maintained in the racing horse population in Korea.</P>