http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Deep learning for stage prediction in neuroblastoma using gene expression data
Park, Aron,Nam, Seungyoon Korea Genome Organization 2019 Genomics & informatics Vol.17 No.3
Neuroblastoma is a major cause of cancer death in early childhood, and its timely and correct diagnosis is critical. Gene expression datasets have recently been considered as a powerful tool for cancer diagnosis and subtype classification. However, no attempts have yet been made to apply deep learning using gene expression to neuroblastoma classification, although deep learning has been applied to cancer diagnosis using image data. Taking the International Neuroblastoma Staging System stages as multiple classes, we designed a deep neural network using the gene expression patterns and stages of neuroblastoma patients. Despite a small patient population (n = 280), stage 1 and 4 patients were well distinguished. If it is possible to replicate this approach in a larger population, deep learning could play an important role in neuroblastoma staging.
Eun-Bi Bae,Il-Kwon Kim,Aron Paek,Hee Yun Park,Seong Eun Jeong 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04
A cDNA (814 bp) encoding ornithine decarboxylase (ODC) antizyme (Az) was cloned from the Tobacco cutworm, Spodoptera litura. The open reading frame (ORF) showed an internal stop codon (TGA) on bases 223-224-225, encoding 270 amino acids except for the 223-T. A modified ODC Az cDNA (Slit ODC AzM) by point mutation for removal of the 223-T was introduced into a yeast (Saccharomyces cerevisiae) strain expressing a cloned ODC from the Tobacco cutworm. The transformed strain with ODC AzM exhibited a remarkable inhibition on ODC action, showing 56 % lowered cellular proliferation rate and the decreased cellular polyamine levels by 40.1 % (putrescine), 64.1 % (spermidine) and 58.6 % (spermine) at 48 hrs of cultivation in comparison with the original ODC Az cDNA. These results suggest that the modified ODC Az is expressed regardless of the frame-shifting and the expressed functional protein effectively inhibits the ODC activity.
Park, Sarah S.,Hendon, Christopher H.,Fielding, Alistair J.,Walsh, Aron,O’Keeffe, Michael,Dincă,, Mircea American Chemical Society 2017 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.139 No.10
<P>The structure-directing role of the inorganic secondary building unit (SBU) is key for determining the topology of metal organic frameworks (MOFs). Here we show that organic building units relying on strong Ir interactions that are energetically competitive with the formation of common inorganic SBUs can also play a role in defining the topology. We demonstrate the importance of the organic SBU in the formation of Mg2H6(H3O)(TTFTB)(3) (MIT-25), a mesoporous MOF with the new ssp topology. A delocalized electronic hole is critical in the stabilization of the TTF triad organic SBUs and exemplifies a design principle for future MOF synthesis.</P>
Park, Sarah S.,Hontz, Eric R.,Sun, Lei,Hendon, Christopher H.,Walsh, Aron,Van Voorhis, Troy,Dincă,, Mircea American Chemical Society 2015 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.137 No.5
<P>Isostructural metal–organic frameworks (MOFs) M<SUB>2</SUB>(TTFTB) (M = Mn, Co, Zn, and Cd; H<SUB>4</SUB>TTFTB = tetrathiafulvalene tetrabenzoate) exhibit a striking correlation between their single-crystal conductivities and the shortest S···S interaction defined by neighboring TTF cores, which inversely correlates with the ionic radius of the metal ions. The larger cations cause a pinching of the S···S contact, which is responsible for better orbital overlap between p<SUB><I>z</I></SUB> orbitals on neighboring S and C atoms. Density functional theory calculations show that these orbitals are critically involved in the valence band of these materials, such that modulation of the S···S distance has an important effect on band dispersion and, implicitly, on the conductivity. The Cd analogue, with the largest cation and shortest S···S contact, shows the largest electrical conductivity, σ = 2.86 (±0.53) × 10<SUP>–4</SUP> S/cm, which is also among the highest in microporous MOFs. These results describe the first demonstration of tunable intrinsic electrical conductivity in this class of materials and serve as a blueprint for controlling charge transport in MOFs with π-stacked motifs.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2015/jacsat.2015.137.issue-5/ja512437u/production/images/medium/ja-2014-12437u_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja512437u'>ACS Electronic Supporting Info</A></P>
Aron Paek,정성은,Hee Yun Park 한국분자세포생물학회 2012 Molecules and cells Vol.33 No.5
Chitinase is a rate-limiting and endo-splitting enzyme involved in the bio-degradation of chitin, an important component of the cuticular exoskeleton and peritrophic matrix in insects. We isolated a cDNA-encoding chitinase from the last larval integument of the cabbage moth, Mamestra brassicae (Lepidoptera; Noctuidae), cloned the ORF cDNA into E. coli to confirm its functionality, and analyzed the deduced amino acid sequence in comparison with previously described lepidopteran chitinases. M. brassicae chitinase expressed in the transformed E. coli cells with the chitinase-encoding cDNA enhanced cell proliferation to about 1.6 times of the untransformed wild type strain in a colloidal chitin-including medium with only a very limited amount of other nutrients. Compared with the wild type strain, the intracellular levels of chitin degradation derivatives, glucosamine and N-acetylglucosamine were about 7.2 and 2.3 times higher, respectively, while the extracellular chitinase activity was about 2.2 times higher in the transformed strain. The ORF of M. brassicae chitinase-encoding cDNA consisted of 1686 nucleotides (562 amino acid residues) except for the stop codon, and its deduced amino acid composition revealed a calculated molecular weight of 62.7 and theoretical pI of 5.3. The ORF was composed of N-terminal leading signal peptide (AA 1-20), catalytic domain (AA 21-392), linker region (AA 393-498), and C-terminal chitin-binding domain (AA 499-562) showing its characteristic structure as a molting fluid chitinase. In phylogenetic analysis, the enzymes from 6 noctuid species were grouped together, separately from a group of 3 bombycid and 1 tortricid enzymes, corresponding to their taxonomic relationships at both the family and genus levels.
Development of a Simple Enzymatic Method for Screening Sucrose Content in Legume Seeds
( Gyutae Kim ),( Aron Park ),( Woon Ji Kim ),( Chang Yeok Moon ),( Byeong Hee Kang ),( Seong-hoon Kim ),( Yu-mi Choi ),( Bo-keun Ha ) 한국육종학회 2021 Plant Breeding and Biotechnology Vol.9 No.3
The soluble sugar content of legume seeds affects the final flavor of the legume and legume products. The purpose of this study was to develop a rapid, simple, and low-cost colorimetric method for high-throughput screening of sucrose content in legume seeds. This colorimetric method was based on the enzymatic reactions of invertase (INV) and glucose oxidase (GOD). Briefly, 20 different soybean and cowpea varieties were used in this study. For sucrose extraction, solvent-to-sample ratios of 10:1 and 5:1 were found to result in optimal absorbance values for determining sucrose content in soybean and cowpea, respectively. The extraction efficiency was also evaluated under various extraction temperatures (25℃ and 50℃) and incubation times (15 minutes, 2 hours, 8 hours, and 24 hours) and the sucrose content was found to increase with increasing temperature and time. Accordingly, the optimal extraction conditions were 24 hours of incubation at 50℃. Under this condition, the GOD/INV method had significant positive correlations (r = 0.91** for soybean and r = 0.87** for cowpea) with the high-performance liquid chromatography method. Overall, this colorimetric method is a fast, simple, and inexpensive tool for quantitative determination of sucrose content in legumes.