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      • KCI등재

        Application of harmonic radar to analyze dispersal behavior of the Japanese pine sawyer beetle, Monochamus alternatus (Coleoptera Cerambycidae)

        Zi-Yi ZHANG,Yu-Ping ZHA,San-ShanCAI,Cheng-Hao HONG,Peng Liang,Jing-Yuan CHEN 한국곤충학회 2020 Entomological Research Vol.50 No.1

        Monochamus alternatus (Hope) is a severe wood-boring pest in coniferous forests and a major vector of pine wilt disease in East Asia. Harmonic radar is a powerful tool for studying the dispersal behavior of insects and it could be applied to control pine wilt disease. In this study, we validated the application of harmonic radar for analyzing the dispersal behavior of M. alternatus beetles in a field environment. We determined the wing capacities of the beetles and the effects of electronic tagging and marking on their movement, flight ability, survivorship, and food consumption in the laboratory to confirm the suitability of this technique. The detection rate and recovery rate for beetles were analyzed separately using radar on caged pine stands and in the field environment. The results showed that the minimum wing capacity of the Japanese pine sawyer was 24.9 mg, which was seven times the weight of the electronic tag (3.5 mg). Marking and tagging the beetles had no significant adverse effects on their movement, flight capacity, food consumption, and survivorship. The detection rate using the radar system and recovery rate based on visual observations of the beetles in caged pines were both 95.6%. However, in the field environment, the detection and recovery rates were only 55.6% and 37.8% after one week, respectively, and 33.3% and 7.8% after two weeks. Harmonic radar is a promising technique for studying the dispersal behavior of the Japanese pine sawyer, but its performance is not satisfactory and major improvements are required for both the radar system and electronic tags.

      • KCI등재

        Automatic Detection and Classification of Rib Fractures on Thoracic CT Using Convolutional Neural Network: Accuracy and Feasibility

        Zhou Qing-Qing,Wang Jiashuo,Tang Wen,Hu Zhang-Chun,Xia Zi-Yi,Xue-Song Li,Zhang Rongguo,Yin Xindao,Zhang Bing,Zhang Hong 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.7

        Objective: To evaluate the performance of a convolutional neural network (CNN) model that can automatically detect and classify rib fractures, and output structured reports from computed tomography (CT) images. Materials and Methods: This study included 1079 patients (median age, 55 years; men, 718) from three hospitals, between January 2011 and January 2019, who were divided into a monocentric training set (n = 876; median age, 55 years; men, 582), five multicenter/multiparameter validation sets (n = 173; median age, 59 years; men, 118) with different slice thicknesses and image pixels, and a normal control set (n = 30; median age, 53 years; men, 18). Three classifications (fresh, healing, and old fracture) combined with fracture location (corresponding CT layers) were detected automatically and delivered in a structured report. Precision, recall, and F1-score were selected as metrics to measure the optimum CNN model. Detection/diagnosis time, precision, and sensitivity were employed to compare the diagnostic efficiency of the structured report and that of experienced radiologists. Results: A total of 25054 annotations (fresh fracture, 10089; healing fracture, 10922; old fracture, 4043) were labelled for training (18584) and validation (6470). The detection efficiency was higher for fresh fractures and healing fractures than for old fractures (F1-scores, 0.849, 0.856, 0.770, respectively, p = 0.023 for each), and the robustness of the model was good in the five multicenter/multiparameter validation sets (all mean F1-scores > 0.8 except validation set 5 [512 x 512 pixels; F1-score = 0.757]). The precision of the five radiologists improved from 80.3% to 91.1%, and the sensitivity increased from 62.4% to 86.3% with artificial intelligence-assisted diagnosis. On average, the diagnosis time of the radiologists was reduced by 73.9 seconds. Conclusion: Our CNN model for automatic rib fracture detection could assist radiologists in improving diagnostic efficiency, reducing diagnosis time and radiologists’ workload.

      • SCIEKCI등재
      • KCI등재

        Electroacupuncture Promotes Motor Function Recovery in MCAO/R Rats by Activating Astrocyte-Related PI3K/AKT Pathway

        Zhang Xiao-Qing,Wang Yi-He,Sun Li,Dong Bao-Qiang,Sui Yue-Jiao,Dong Jia-Zi,Han Yang 사단법인약침학회 2022 Journal of Acupuncture & Meridian Studies Vol.15 No.5

        Background: Electroacupuncture (EA) is a widely used traditional Chinese medicine method to manage various diseases, including cerebral ischemia-reperfusion injury (CIRI). Objectives: We assessed the neuroprotective effects of EA and examined its mechanism in a rat model of the middle cerebral artery occlusion-reperfusion (MCAO/R). The gait analysis was performed to evaluate the neuroprotective effects. Western blot and immunohistochemistry assays were carried out to determine the molecular mechanisms of EA. Methods: Male SD rats were randomly divided into the sham operation group, right MCAO/R group, and EA group. EA was administered every day (4/20 Hz, 10 min/1 d) at the following acupoints: Baihui (DU20), Yintang (EX-HN3), and Zusanli (ST36). Gait and motor function were analyzed from day 8 onward. Results: The plantar support and balance coordination of MCAO/R rats decreased, and the cellular structure of the ischemic penumbra was unclear. EA improved the gait dynamics of the rats, adjusted the cell structure, further activated astrocytes, and increased the expression and phosphorylation of phosphoinositide 3-kinase/protein kinase B (PI3K/PKB or AKT). Conclusion: EA promoted astrocyte-related effects in the rat model. Our findings suggest that the neuroprotective mechanism of EA may be related to the activation of the PI3K/ AKT signaling pathway. The intervention enhanced brain protection and improved motor functions.

      • KCI등재

        Identification and expression analysis of the E2F/DP genes under salt stress in Medicago truncatula

        Tian-Yi Ma,Jing Guo,Zi-Wei Li,Shu-Yong Zhang,Guo-Ting Liang 한국유전학회 2014 Genes & Genomics Vol.36 No.6

        Cell cycle control in plants converges on theRb/E2F/DP pathway, which is regulated by cyclin-dependentkinases. Plants can coordinate their cell cycles duringsalt stress to benefit their growth and development. However,the mechanism underlying cell cycle control undersalt stress is still unclear. Here, we identified five predictedE2F/DP genes in the Medicago truncatula genome,including three E2Fs, one DP, and one DEL. The conserveddomains of the E2F/DP proteins were relativelywell conserved with those of Arabidopsis thaliana andOryza sativa. Intron/exon organization analyses indicatedthat Medtr;E2Fc and Medtr;DPa gained/lost introns in theconserved domains during recent evolutionary process. Furthermore, an expression analysis showed that thesegenes were expressed with varied transcription levels in allof the tissues tested. Contrasting gene expression changesin response to salt treatment in salt-tolerant versus saltsensitivegenotypes indicated that Medtr;DPa might be acandidate gene underlying the salt tolerance. This studywill contribute to the understanding of the E2F/DP transcriptionfactors in M. truncatula and of the mechanismorganizing cell cycle regulation and salt stress.

      • KCI등재

        Refolding with Simultaneous Purification of Recombinant Core Streptavidin Using Single-step High-performance Hydrophobic Interaction Chromatography

        Siyao Wang,Yuejuan Zhang,Dong Gao,Jing Zi,Wenpeng Wang,Nianzhe Zhang,Yi Wan,Lili Wang 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.4

        Streptavidin has applied to many areas including detection, purification, labeling, crosslinking and immobilization resulting in a high demand on its production. In this study, we report a method for preparation of recombinant core streptavidin (cSAV) protein using highperformance hydrophobic interaction chromatography (HPHIC). Firstly the cSAV was successfully cloned and expressed in Escherichia coli as inclusion bodies. A bifunctional stationary phase mainly working as HIC mode accompanied by weak anion exchange chromatography (WAX) was prepared using β-phenylethylamine (PEA) as a ligand. The denatured cSAV was then refolded and simultaneously purified by PEA hydrophobic interaction chromatography (PEA-HIC). The mass recovery and purity of cSAV by single-step were 30.2% and 98%, respectively. The bioactivity was determined to be 13.2 U/mg by biotin binding capacity assay. This method provides a new possibility for fast separation with simultaneous renaturation of cSAV.

      • SCIESCOPUSKCI등재

        Mode Switching Smooth Control of Transient Process of Grid-Connected 400 Hz Solid-State Power Supply System

        Zhu, Jun-Jie,Nie, Zi-Ling,Zhang, Yin-Feng,Han, Yi The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.6

        The mode-switching control of transient process is important to grid-connected 400 Hz solid-state power supply systems. Therefore, this paper analyzes the principle of on-grid and islanding operation of the system with or without local loads in the grid-connected process and provides a theoretical study of the effect of different switching sequences on the mode-switching transient process. The conclusion is that the mode switch (MS) must be turned on before the solid-state switch (STS) in the on-grid process and that STS must be turned off before the MS in the off-grid process. A strategy of mode-switching smooth control for transient process of the system is proposed, including its concrete steps. The strategy utilizes the average distribution of peak currents and the smooth adjustment of peak currents and phases to achieve a no-shock grid connection. The simulation and experimental results show that the theoretical analysis is correct and that the method is effective.

      • SCIESCOPUSKCI등재

        Mode Switching Smooth Control of Transient Process of Grid-Connected 400 ㎐ Solid-State Power Supply System

        Jun-Jie Zhu,Zi-Ling Nie,Yin-Feng Zhang,Yi Han 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.6

        The mode-switching control of transient process is important to grid-connected 400 ㎐ solid-state power supply systems. Therefore, this paper analyzes the principle of on-grid and islanding operation of the system with or without local loads in the grid-connected process and provides a theoretical study of the effect of different switching sequences on the mode-switching transient process. The conclusion is that the mode switch (MS) must be turned on before the solid-state switch (STS) in the on-grid process and that STS must be turned off before the MS in the off-grid process. A strategy of mode-switching smooth control for transient process of the system is proposed, including its concrete steps. The strategy utilizes the average distribution of peak currents and the smooth adjustment of peak currents and phases to achieve a no-shock grid connection. The simulation and experimental results show that the theoretical analysis is correct and that the method is effective.

      • SCIESCOPUSKCI등재

        Two Maternal Lineages Revealed by Mitochondrial DNA D-loop Sequences in Chinese Native Water Buffaloes (Bubalus bubalis)

        Lei, Chu-Zhao,Zhang, Wei,Chen, Hong,Lu, Fan,Ge, Qing-Lan,Liu, Ruo-Yu,Dang, Rui-Hua,Yao, Yun-Yi,Yao, Li-Bo,Lu, Zi-Fan,Zhao, Zhong-liang Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.4

        Little is known about the origin and genetic diversity of swamp buffaloes in China. To obtain more knowledge on genetics of the water buffalo in China, the complete mitochondrial D-loop sequences of 30 samples from 6 native types were investigated. The results revealed 12 mitochondrial haplotypes with 50 polymorphic sites. Among these polymorphic sites, there were 49 transitions and 1 transversion. The average nucleotide diversity and haplotype diversity estimated from mtDNA D-loop region in 6 Chinese water buffalo types were 0.00684 and 0.798, respectively, showing rather abundant mitochondrial genetic diversity. The Neighbor-Joining (NJ) tree of mtDNA of Chinese water buffaloes was constructed according to the 12 haplotypes. The NJ tree indicated two lineages being designated lineage A and lineage B, in which lineage A was predominant, and lineage B was at low frequency. The new lineage B was first discovered and defined in 6 Chinese water buffalo types. These results showed that two different maternal lineages were involved in the origin of domestic swamp buffaloes in China and the lineage B was probably an introgression from Southeast Asian buffaloes.

      • KCI등재

        Management strategy for Spodoptera frugiperda (Lepidoptera:Noctuidae) in mountain maize planting areas in China

        Yao Yao,He Yanyan,Zhang Lilan,Wang Kun,Li Bin,Du Guangzu,Zhu Chunyue,Yi Xiaocui,Liu Yiyi,Zi Shengjiang,Chen Bin,Xiao Guanli 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4

        The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is a major maize pest worldwide and has caused huge economic loss. To explore the control tactics of S. frugiperda in mountain maize planting fields, studies were performed in China from 2020 to 2021 for screening the S. frugiperda-resistant maize variety, seed coating agents, chemical insecticides, and biological agents. The results showed that 75 g/hm 2 Emamectin benzoate had better controlling efficacy (with a control efficacy of 91.05% at 1d) than other chemicals, so it was recommended in the high incidence period of pests. Compared with other seed coating treatments, 50% Lumi ngwei TM625FS (with a control efficacy of 76.19% at 10d) was the best seed coating against S. frugiperda. The biological control “Telenomus remus + Picromerus lewisi” and “T. remus + Eocanthecona furcellata” had good control efficacy on S. frugiperda with 76.01%, 64.10% at 20d, respectively. Additionally, there were lower numbers of S. frugiperda in maize variety GuiDan6, BaoYu9, and YanBaiYu8. In summary, this research rec ommended four effective pest management strategies (GuiDan6-resistant maize variety, 50% Lumingwei TM625FS-seed coating agents, 5% emamectin benzoate-chemical pesticide, and “T. remus + P. lewisi”-biological control) in mountain maize planting fields. The study provided effective control strategies for

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