Ziwuliuzhu Acupuncture Modulates Clock mRNA, Bmal1 mRNA and Melatonin in Insomnia Rats

Huang Ao,Xiao Gefang,Chen Yiliu,Hu Zuying,Lee Pin-Hsuan,Huang Yusen,Zhuang Zifeng,Zhang Yuling,Qing Peng,Zhao Canghuan 사단법인약침학회 2023 Journal of Acupuncture & Meridian Studies Vol.16 No.3

Background: In clinics, Ziwuliuzhu acupuncture is widely considered an effective method of treating insomnia; however, there is currently limited information available regarding its possible mechanisms. Although the method of Ziwuliuzhu acupuncture possesses a unique rhythmic pattern. Objectives: In this study, we have creatively combined the traditional Chinese medicine of Ziwuliuzhu with a modern biological rhythm to investigate the internal mechanism of insomnia. Methods: Pathological tissue from the hypothalamus was analyzed using hematoxylin– eosin staining. The level of TNF (tumor necrosis factor)-α in the SCN (suprachiasmatic nucleus) area of the hypothalamus was detected in situ using the TUNEL fluorescence staining assay. The concentration of hypothalamic melatonin was detected using the enzyme-linked immunosorbent assay (ELISA). The mRNA expression of Clock and Bmal1 was measured using RT-qPCR. Results: In the Ziwuliuzhu acupuncture groups, the structural damage in the hypothalamic neurons was alleviated compared to the model group and the expression of inflammatory factors was reduced. The mRNA expression levels of Clock and Bmal1 were significantly increased (p < 0.05). The concentration of melatonin was significantly increased (p < 0.001). Although there were no significant differences between the treatment groups (diazepam group, Nazi group, Najia group, and routine group) (p > 0.05). Conclusion: Ziwuliuzhu acupuncture alleviated neuronal damage and modulated the inflammatory reaction in the hypothalamus of rats with insomnia. Moreover, Ziwuliuzhu acupuncture increased the expression levels of Clock and Bmal1 mRNA, and MT content. This study has potentially highlighted one of

Novel DNAH1 Mutation Loci Lead to Multiple Morphological Abnormalities of the Sperm Flagella and Literature Review

Zhuang Bao-Jun,Xu Su-Yun,Dong Liang,Zhang Pei-Hai,Zhuang Bao-Lin,Huang Xiao-Peng,Li Guang-Sen,You Yao-Dong,Chen Di'Ang,Yu Xu-Jun,Chang De-Gui 대한남성과학회 2022 The World Journal of Men's Health Vol.40 No.4

The protein encoded by dynein axonemal heavy chain 1 (DNAH1) is a part of dynein, which regulates the function of cilia and sperm flagella. The mutant of DNAH1 causes the deletion of inner dynein arm 3 in the flagellum, leading to multiple morphological abnormalities of the sperm flagella (MMAF) and severe asthenozoospermia. However, instead of asthenozoospermia and MMAF, the result caused by the mutation of DNAH1 remains unknown. Here we report a male infertility patient with severe asthenozoospermia and teratozoospermia. We found two heterozygous mutations in DNAH1 (c.6912C>A and c.7076G>T) and which were reported to be associated with MMAF for the first time. We next collected and analyzed 65 cases of DNAH1 mutation and found that the proportion of short flagella is the largest, while the bent flagella account for the smallest, and the incidence of head deformity is not high in the sperm of these patients. Finally, we also analyzed 31 DNAH1 mutation patients who were treated with intracytoplasmic sperm injection (ICSI) and achieved beneficial outcomes. We hope our research will be helpful in the diagnosis and treatment of male infertility caused by DNAH1 mutation.

Spatial Regeneration for Preservation of Historic Urban Features and Improvement of Living Conditions in High-density Historic Districts: Urban Renewal Design Strategies for Shanghai's Old Town

Huang, Na,Cai, Yongjie,Zhuang, Mingyu,Zhou, Yi,Zhou, Jun Council on Tall Building and Urban Habitat Korea 2021 International journal of high-rise buildings Vol.10 No.1

Being the origin of Shanghai City, the Old Town presents a high-density spatial texture, a characteristic of local living conditions in the Shanghai context. However, the Old Town is faced with competing interests: the preservation of historic urban features and the improvement of contemporary living conditions. In view of its high density and poor living conditions, this paper focuses on two types of blocks for urban design research, and proposes two spatial regeneration strategies, as "overlapping lilong" and "texture continuity". It is expected to inherit the regional characteristics of urban space, improve the plot ratio and supplement the mix of functions, through the translation of the traditional lilong typology and the reproduction of historical streets and alleys, so as to provide operable spatial strategies and design methods for the organic renewal of Old Town and other historic districts.

Comparative Study and Electrochemical Properties of LiFePO₄F Synthesized by Different Routes

Huang, Bin,Liu, Suqin,Li, Hongliang,Zhuang, Shuxin,Fang, Dong Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.7

To improve the performance of $LiFePO_4F$, a novel sol-gel process is developed. For comparison, ceramic process is also implemented. From X-ray diffraction results we know that each sample adopts a triclinic $P{\bar{1}}$ space group, and they are isostructural with amblygonite and tavorite. The scanning electron microscope images show that the homogeneous grains with the dimension of 300-500 nm is obtained by the sol-gel process; meanwhile the sample particles obtained by ceramic process are as big as 1000-3000 nm. By galvanostatic tests and at electrochemical impedance spectroscopy method, the sample obtained by sol-gel process presents better electrochemical properties than the one obtained by ceramic process.

Bioprospecting of Novel and Bioactive Metabolites from Endophytic Fungi Isolated from Rubber Tree Ficus elastica Leaves

( Zhuang Ding ),( Tao Tao ),( Lili Wang ),( Yanna Zhao ),( Huiming Huang ),( Demeng Zhang ),( Min Liu ),( Zhengping Wang ),( Jun Han ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.5

Endophytic fungi are an important component of plant microbiota, and have the excellent capacity for producing a broad variety of bioactive metabolites. These bioactive metabolites not only affect the survival of the host plant, but also provide valuable lead compounds for novel drug discovery. In this study, forty-two endophytic filamentous fungi were isolated from Ficus elastica leaves, and further identified as seven individual taxa by ITS-rDNA sequencing. The antimicrobial activity of these endophytic fungi was evaluated against five pathogenic microorganisms. Two strains, Fes1711 (Penicillium funiculosum) and Fes1712 (Trichoderma harzianum), displayed broad-spectrum bioactivities. Our following study emphasizes the isolation, identification and bioactivity testing of chemical metabolites produced by T. harzianum Fes1712. Two new isocoumarin derivatives (1 and 2), together with three known compounds (3-5) were isolated, and their structures were elucidated using NMR and MS. Compounds 1 and 2 exhibited inhibitory activity against Escherichia coli. Our findings reveal that endophytic fungi from the rubber tree F. elastica leaves exhibit unique characteristics and are potential producers of novel natural bioactive products.

Comparative Study and Electrochemical Properties of LiFePO4F Synthesized by Different Routes

Bin Huang,Suqin Liu,Hongliang Li,Shuxin Zhuang,Dong Fang 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.7

To improve the performance of LiFePO4F, a novel sol-gel process is developed. For comparison, ceramic process is also implemented. From X-ray diffraction results we know that each sample adopts a triclinic P1 space group, and they are isostructural with amblygonite and tavorite. The scanning electron microscope images show that the homogeneous grains with the dimension of 300-500 nm is obtained by the sol-gel process; meanwhile the sample particles obtained by ceramic process are as big as 1000-3000 nm. By galvanostatic tests and at electrochemical impedance spectroscopy method, the sample obtained by sol-gel process presents better electrochemical properties than the one obtained by ceramic process.

Circular RNA hsa_circ_0075828 promotes bladder cancer cell proliferation through activation of CREB1

( Chengle Zhuang ),( Xinbo Huang ),( Jing Yu ),( Yaoting Gui ) 생화학분자생물학회(구 한국생화학분자생물학회) 2020 BMB Reports Vol.53 No.2

Circular RNAs (circRNAs), one kind of non-coding RNA, have been reported as critical regulators for modulating gene expression in cancer. In this study, microarray analysis was used to screen circRNA expression profiles of bladder cancer (BC) 5637 cells, T24 cells and normal control SV-HUC-1 cells. The data from the microarray showed that hsa_circ_0075828 (named circCASC15) was most highly expressed in 5637 and T24 cells. circCASC15 was highly expressed in BC tissues and cells. Overexpression of circCASC15 was closely associated with BC tumor stage and promoted cell proliferation significantly in vitro and in vivo. Mechanistically, circCASC15 could act as miR-1224-5p sponge to activate the expression of CREB1 to promote cell proliferation in BC. In short, circCASC15 promotes cell proliferation in BC, which might be a new molecular target for BC diagnosis and therapy. [BMB Reports 2020; 53(2): 82-87]

SALT-INDUCED CHLOROPLAST PROTEIN (SCP) is Involved in Plant Tolerance to Salt Stress in Arabidopsis

Yong Zhuang,Yangxuan Liu,Yuxiang Li,Ming Wei,Yuying Tang,Penghui Li,Zhijian Liu,Hui Li,Weizao Huang,Songhu Wang 한국식물학회 2019 Journal of Plant Biology Vol.62 No.6

Soil salinization threats the agricultural productionand food security worldwide. Salt stress induced plantsenescence and chloroplast degradation. However, it remainslargely unknown how the chloroplast-localized proteins affectplant response to salt stress. Here, we characterized a novelgene (At5g39520) in Arabidopsis, which is induced by saltstress and encodes a chloroplast-localized protein. Thus, thisgene was named SALT-INDUCED CHLOROPLAST PROTEIN(SCP). A T-DNA insertion mutant of SCP gene (scp-1)showed the enhanced tolerance to salt stress, as indicated bythe increased survival rates, fresh weights and chlorophyllcontents compared with wild type plants under salt treatment. Salt-induced leaf senescence was also delayed in scp-1 mutant. The scp-1 complementation line and SCP overexpressionlines displayed the hypersensitivity to salt stress. The qRTPCRanalysis indicated that the transcripts of CHLOROPLASTVESICULATION (CV), which mediates stress-inducedchloroplast degradation, were altered in scp-1 mutant andSCP overexpression lines. Taken together, our results suggestthat SCP gene plays a negative role in response to salt stress andhas potential application for genetic modification of improvingplant tolerance to salt stress.

Substituent Effects of Accelerator on Nitroxide-Mediated Radical Polymerization

Jian-ying Huang,Jia-ming Zhuang,You-si Zou 한국고분자학회 2011 Macromolecular Research Vol.19 No.8

The acceleration effect of various agents on the rate of styrene bulk polymerization in 2,2,6,6-tetramethylpiperidinyloxy (TEMPO) mediated polymerization was investigated, including dimethyl malonate (DMM), diethyl methyl malonate (DEMM), diethyl tert-butylmalonate (DEBM), diethyl diethylmalonate (DEDEM), 3-methyl-2,4-pentanedione (MPD), acetyl malononitrile (Ac-MN), and dimethyl malononitrile (DM-MN). Polymerization with the additive proceeded in a living manner, as indicated by keeping a low polydispersity and increasing the molecular weight with the reaction time and conversion. The structure of the styrene polymerization did not change in the presence of these additives. The monomer conversion efficiency was approximately 99% and maintained a relatively narrow polydispersity of 1.29 with the optimal [Ac-MN]/[TEMPO] molar ratios of 4 in 1.5 h. With the accelerator,dipole/dipole interactions led to a weakening of the C-ON bond and an acceleration of the reaction. There is a trend for a higher polymerization rate with more electron-withdrawing substituents.

重混时代下中国重混创作权的构建研究

黄德姬(Deji Huang),庄佩茹(Peiru Zhuang) YIXIN 출판사 2023 Journal of China Studies Vol.1 No.1

随着数字网络技术的发展,公众对于作品的使用、创作、传播能力进一步增强。数字网络技术为获取作品带来新的手段与方式,也进一步引发了创作者对已有的文字、音乐、视频等作品进行重新组合创作新作品的重混文化现象。重混作品在促进文化多元化的同时也引发了许多著作权问题,而目前中国著作权法对重混行为并没有明确规定,这不利于保护重混创作者的创作积极性。因此本文从具体司法案例出发分析现有著作权保护的不足,并尝试提出将重混作品纳入著作权法保护范围、为重混创作者设立重混创作权、引入知识共享协议等建议,以平衡权利人与使用者间的利益分享,构建与重混创作相适应的法律制度。 With the development of digital network technology, the public’s ability to use, create, and disseminate works has further enhanced. Digital network technology has brought new means and methods to obtain works, and has further triggered the phenomenon of remixing culture where creators recombine existing works such as text, music, and videos to create new works. While promoting cultural diversity, remixed works have also raised many copyright issues. Currently, China’s copyright law does not explicitly regulate remixed behavior, which is not conducive to protecting the creative enthusiasm of remixed creators. Therefore, this paper analyzes the shortcomings of existing copyright protection from specific judicial cases, and attempts to propose suggestions such as incorporating remixed works into the scope of copyright law protection, establishing remixed creation rights for remixed creators, and introducing knowledge sharing agreements. A legal system suitable for remixed creation should be constructed in order to balance the benefit sharing between rights holders and users.