Ziwuliuzhu Acupuncture Modulates Clock mRNA, Bmal1 mRNA and Melatonin in Insomnia Rats

Huang Ao,Xiao Gefang,Chen Yiliu,Hu Zuying,Lee Pin-Hsuan,Huang Yusen,Zhuang Zifeng,Zhang Yuling,Qing Peng,Zhao Canghuan 사단법인약침학회 2023 Journal of Acupuncture & Meridian Studies Vol.16 No.3

Background: In clinics, Ziwuliuzhu acupuncture is widely considered an effective method of treating insomnia; however, there is currently limited information available regarding its possible mechanisms. Although the method of Ziwuliuzhu acupuncture possesses a unique rhythmic pattern. Objectives: In this study, we have creatively combined the traditional Chinese medicine of Ziwuliuzhu with a modern biological rhythm to investigate the internal mechanism of insomnia. Methods: Pathological tissue from the hypothalamus was analyzed using hematoxylin– eosin staining. The level of TNF (tumor necrosis factor)-α in the SCN (suprachiasmatic nucleus) area of the hypothalamus was detected in situ using the TUNEL fluorescence staining assay. The concentration of hypothalamic melatonin was detected using the enzyme-linked immunosorbent assay (ELISA). The mRNA expression of Clock and Bmal1 was measured using RT-qPCR. Results: In the Ziwuliuzhu acupuncture groups, the structural damage in the hypothalamic neurons was alleviated compared to the model group and the expression of inflammatory factors was reduced. The mRNA expression levels of Clock and Bmal1 were significantly increased (p < 0.05). The concentration of melatonin was significantly increased (p < 0.001). Although there were no significant differences between the treatment groups (diazepam group, Nazi group, Najia group, and routine group) (p > 0.05). Conclusion: Ziwuliuzhu acupuncture alleviated neuronal damage and modulated the inflammatory reaction in the hypothalamus of rats with insomnia. Moreover, Ziwuliuzhu acupuncture increased the expression levels of Clock and Bmal1 mRNA, and MT content. This study has potentially highlighted one of

SOCIAL CHAOS

황차오 ( Huang Ch Ao ) 한국기초조형학회 2019 한국기초조형학회 작품집 Vol.2019 No.1

SARSEvaluation of CRISPR-Based Assays for Rapid Detection of SARS-CoV-2: A Systematic Review and Meta-Analysis

Pei-Ying Huang,Xin Yin,Yue-Ting Huang,Qi-Qing Ye,Si-Qing Chen,Xun-Jie Cao,Tian-Ao Xie,Xu-Guang Guo 연세대학교의과대학 2022 Yonsei medical journal Vol.63 No.5

Purpose: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the pathogen of coronavirus disease 2019. Diagnosticmethods based on the clustered regularly interspaced short palindromic repeats (CRISPR) have been developed to detect SARSCoV-2 rapidly. Therefore, a systematic review and meta-analysis were performed to assess the diagnostic accuracy of CRISPR fordetecting SARS-CoV-2 infection. Materials and Methods: Studies published before August 2021 were retrieved from four databases, using the keywords “SARS-CoV-2”and “CRISPR.” Data were collected from these publications, and the sensitivity, specificity, negative likelihood ratio (NLR), positivelikelihood ratio (PLR), and diagnostic odds ratio (DOR) were calculated. The summary receiver operating characteristic curve wasplotted for analysis with MetaDiSc 1.4. The Stata 15.0 software was used to draw Deeks’ funnel plots to evaluate publication bias. Results: We performed a pooled analysis of 38 independent studies shown in 30 publications. The reference standard was reversetranscription-quantitative PCR. The results indicated that the sensitivity of CRISPR-based methods for diagnosis was 0.94 (95% CI0.93–0.95), the specificity was 0.98 (95% CI 0.97–0.99), the PLR was 34.03 (95% CI 20.81–55.66), the NLR was 0.08 (95% CI 0.06–0.10), and the DOR was 575.74 (95% CI 382.36–866.95). The area under the curve was 0.9894. Conclusion: Studies indicate that a diagnostic method based on CRISPR has high sensitivity and specificity. Therefore, this wouldbe a potential diagnostic tool to improve the accuracy of SARS-CoV-2 detection.

Reliable Mobility Evaluation of Organic Field-Effect Transistors With Different Contact Metals

Huang, Fanming,Liu, Ao,Zhu, Huihui,Xu, Yong,Balestra, Francis,Ghibaudo, Gerard,Noh, Yong-Young,Chu, Junhao,Li, Wenwu IEEE 2019 IEEE electron device letters Vol.40 No.4

<P>The reliability of mobility evaluation of organic field-effect transistors (OFETs) is a serious issue because numerous overestimated and underestimated mobilities have been reported recently. A reliable approach to accurately evaluate the OFET mobility is therefore highly desirable. Here, in this letter, two commonly employed methods and the Y function method (YFM) were used to extract the mobilities of indacenodithiophene-co-benzothiadiazole (IDT-BT) OFETs with four different contact metals. The mobilities extracted by the commonly used methods varied greatly with the contact metal and channel length, whereas those extracted by the YFM were very stable and approached the intrinsic mobility of IDT-BT. Our results show that YFM is a precise approach that can be widely used to evaluate the OFET mobility.</P>

SOX11-dependent CATSPER1 expression controls colon cancer cell growth through regulation the PI3K/AKT signaling pathway

Huang Yang,Wang Yicheng,Wu Zhongmei,Li Tao,Li Shupei,Wang Chan,Ao Jine,Yang Changming,Zhou Yu 한국유전학회 2022 Genes & Genomics Vol.44 No.11

Background: Colorectal cancer (CRC) is one of the most common malignant tumors and the fourth leading cause of cancer death worldwide. Constitutive activation of the PI3K/AKT signaling pathway is a hallmark of colon tumor growth. CATSPER1 gene encodes a pore-forming and pH-sensing subunit of the CatSper Ca2+-permeable channel, a sperm-specific calcium channel essential for hyperactivated motility and male fertility. However, the function of CATSPER1 outside the male reproductive system is unclear. Objective: This study was designed to explore whether CatSper exerted its functional role in the progress of CRC, and investigate the possible mechanisms. Methods: Microarray data (GSE146587) from 6 patients diagnosed with stage III CRC post-surgery was analyzed by Limma R package. The Kaplan Meier plotter (KM plotter) database was used to assess the relevance of CATSPER1 mRNA expression to the overall survival (OS) rates in CRC. Western blot, real-time PCR and luciferase reporter assays were used to determine the SOX11-CATSPER1 axis in CRC cells. Clustered regularly interspaced short palindromic repeats (CRISPR)-based gene editing was used to generate CATSPER1 knockout (KO) CRC cells. The proliferation of CRC cells was determined by BrdU incorporation and colony formation assays. The effect of CATSPER1 on CRC tumor growth in vivo was investigated in a mice tumor xenograft model. Results: Here, we show that CATSPER1 expression was significantly up-regulated in CRC and elevated CATSPER1 was associated with poor overall survival (OS). Moreover, the transcription factor SOX11 (SRY-related high-mobility-group (HMG) box 11) activated CATSPER1 transcription in CRC cells. Functionally, we showed that CATSPER1 promoted CRC cells proliferation both in vitro and in vivo. At the molecular level, we demonstrated that CATSPER1 might maintain CRC malignant process partly through the activation of the PI3K/AKT signaling pathway. Conclusion: Increased CATSPER1 expression facilitates CRC cells proliferation, suggesting that targeting CATSPER1 might represent a promising strategy for colon cancer treatment.

Analysis of Co-Relation Between Objective Measurement and Subjective Assessment for Dynamic Comfort of Vehicles

Ao Di,Wong Pak Kin,Huang Wei,Mei Xing Tai,Cao Yu Cong,Zhao Jing 한국자동차공학회 2020 International journal of automotive technology Vol.21 No.6

The assessment of vehicle dynamic comfort to human body vibration varies from person to person. Even though the dynamic comfort rating can be obtained by placing measurement devices over the cabin, passengers may have dissimilar sensations. For many car manufacturers, both subject and objective measurements are used together at development of new cars. To effectively assess the dynamic comfort of vehicle, this research explores the correlation between the objective measurement of the whole body vibration, hand-transmitted vibration and subjective assessment. The whole body vibration is measured from the backrest, seat surface and vehicle floor, whereas the hand-transmitted vibration is measured from the steering wheel. Four different vehicles are driven at various speeds on five types of pavements. Both simple linear regression and polynomial regression models based on Stevens’s power law from psychophysics are built to correlate the subjective and objective data. The regression results show that (1) the seat surface is more reliable than other positions for assessing the human body vibration; (2) the hand transmitted vibration on rough roads is more trustful for dynamic comfort evaluation than on smooth roads; (3) the overall dynamic comfort value is the most reliable index for dynamic comfort evaluation.

Experimental Investigation on Fatigue Improvement of Orthotropic Steel Bridge Deck Using Steel Fiber Reinforced Concrete

Huawen Ye,Ao Huang,Chengchuan Jiang,Wenchao Wang 한국콘크리트학회 2023 International Journal of Concrete Structures and M Vol.17 No.5

The high-performance topping concrete overlay would significantly reduce the fatigue stresses at susceptible details of orthotropic steel deck (OSD) and effectively improve the fatigue performance. Full-scale fatigue tests and numerical simulations were carried out to quantify the effects of steel fiber reinforced concrete (SFRC) topping pavement in reducing the OSD fatigue stresses based on a practical bridge design. Fatigue stress of the OSD critical details was measured from tests and cracking was observed in the SFRC overlay. Both an innovative pre-pump-pulse Brillouin optical time domain analysis (PPP-BOTDA) distributed optical fiber sensor and strain gauges were also used to measure the stress at OSD fatigue-prone details, including the crossbeam cut-out, rib-to-deck weld, rib-to-crossbeam weld and splice of U-rib. The corresponding three-dimensional (3-D) finite element model was then established and verified by the test results, and used to conduct a parametric study to obtain a quantitative expression of fatigue stress reduction factor, in terms of relative elastic modulus and thickness of overlay and steel deck plate. The proposed quantitative model of the fatigue stress reduction effect can guide the cost-effective design of composite bridge deck.

Endophytic Fungal strains Specifically Modified the Biochemical Status of Grape Cells

Li-Hua Huang,Ming-Zhi Yang,Xiu-Jin Ao,An-Yun Ren,Ming-Quan Yuan,Han-Bo Zhang 한국식물학회 2018 Journal of Plant Biology Vol.61 No.4

Previously, specific interactions in morphologywere observed between grape cells and endophytic fungalstrains during a dual culture experiment. However, thebiochemical impacts of these fungal endophytes on grapecells is also expected due to their potential application in grapequality management. After assessed multiple physiochemicaltraits to those grape cells which have co-cultured with differentendophytic fungal strains in this study, and found the presenceof fungal endophytes obviously triggered ROS stress responsesin grape cells, and the biochemical status in grape cells weredifferentially modified by different fungal strains. In thosetested endophytic fungal strains, RH37 (Epicoccum sp.),RH6 (Alternaria sp.), RH32 (Alternaria sp.) and RH34(Trichothecium sp.) conferred greater metabolic impacts ongrape cells. And soluble protein (SPr), total flavonoids (TF),total phenols (TPh) and malondialdehyde (MDA) on the otherhand, were sensitive biochemical parameters which can beinfluenced in greater ranges than other detected parameters. Most interestedly, fungal endophytes shaped metabolitespatterns in grape cells during the dual culture appeared fungalgenus/species/strain-specificity. The work confirmed thesignificance of fungal endophytes in grape metabolic regulationand elucidated the possibility to purposely manage grapequality using tool of fungal endophytes.

Early gut microbiota in very low and extremely low birth weight preterm infants with feeding intolerance: a prospective case-control study

Liu Ling,Ao Dang,Cai Xiangsheng,Huang Peiyi,Cai Nali,Lin Shaozhu,Wu Benqing 한국미생물학회 2022 The journal of microbiology Vol.60 No.10

The potential role of the gut microbiota in the pathogenesis of feeding intolerance (FI) remains unclear. Understanding the role of the gut microbiota could provide a new avenue for microbiota-targeted therapeutics. This study aimed to explore the associations between aberrant gut microbiota and FI in very low or extremely low birth weight (VLBW/ELBW) preterm infants. In this observational case-control study, VLBW/ ELBW infants were divided into two groups: FI group and feeding tolerance (FT) group. 16S rRNA gene sequencing was performed to analyze the gut microbial diversity and composition of the infants. The differences in the gut microbiota of the two groups were compared. In total, 165 stool samples were obtained from 44 infants, among which, 31 developed FI and 13 served as controls. Alpha diversity was the highest in the meconium samples of the two groups. LEfSe analysis revealed that the abundances of Peptostreptococcaceae, Clostridiales and Clostridia in the FT group were significantly higher than in the FI group. At the phylum level, the FI group was dominated by Proteobacteria, and the FT group was dominated by Firmicutes. The meconium samples of the FI group had higher proportions of γ-proteobacteria and Escherichia-Shigella and a lower proportion of Bacteroides compared with the FT group. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that aberrant gut bacteria in the FI group were strongly associated with dysregulation of C5-Brancheddibasic- acid-metabolism, protein kinases, and sporulation. These findings reveal candidate microbial markers to prevent FI. Increased relative abundances of γ-proteobacteria and Escherichia-Shigella and decreased abundance of Bacteroides in meconium were associated with an increased risk of FI, while Peptostreptococcaceae, Clostridiales and Clostridia reduced the risk of FI in VLBW/ELBW infants.

IDENTIFICATION OF SINGLE VARIABLE CONTINUITY LINEAR SYSTEMS WITH STABILITY CONSTRAINTS FROM SAMPLES OF INPUT-OUTPUT DATA

Zhao-Qing Huang,Jian-Feng Ao 제어로봇시스템학회 1991 제어로봇시스템학회 국내학술대회 논문집 Vol.1 No.2