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Serum Macrophage Migration Inhibitory Factor as a Biomarker of Active Pulmonary Tuberculosis
Zhong-bo Shang,Jun Wang,Shou-gang Kuai,Yin-yin Zhang,Qin-fang Ou,Hao Pei,Li Hua Huang 대한진단검사의학회 2018 Annals of Laboratory Medicine Vol.38 No.1
Background: Macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine with chemokine-like functions, has been shown to play a central role in several acute and chronic inflammatory diseases. However, limited information is available regarding the use of MIF as an inflammatory pathway marker in patients with tuberculosis. This study aimed to investigate the association of MIF with IFN-γ and TNF-α in active pulmonary tuberculosis (APTB) following anti-tuberculosis treatment. Methods: The MIF, TNF-α, and IFN-γ serum levels were determined in 47 patients with APTB by cytokine-specific ELISA at four phases: prior to anti-tuberculosis drug treatment (baseline), and following 2, 4, and 6 months of treatment. In addition, we measured the MIF, TNF-α, and IFN-γ serum levels in 50 health controls. Results: MIF serum levels were significantly elevated (P<0.05) in patients with APTB prior to treatment compared with that in control subjects, and TNF-α ≥449.7 pg/mL was associated with high MIF levels (≥13.1 ng/mL). MIF levels were significantly reduced (P<0.01) following 2, 4, and 6 months of treatment, with variations in TNF-α and IFN-γ serum levels. MIF levels were positively correlated with the paired TNF-α level at baseline (r=0.1103, P=0.0316) and following 6 months of treatment (r=0.09569, P=0.0364).Conclusions: A reduction in the MIF serum levels in patients with APTB following anti-tuberculosis treatment may positively affect host immune protection against Mycobacterium tuberculosis infection. Thus, serum MIF levels may constitute a useful marker for assessing therapy effectiveness in patients with APTB.
Molecular Cloning and Bioinformatic Analysis of SPATA4 Gene
Liu, Shang-Feng,Ai, Chao,Ge, Zhong-Qi,Liu, Hai-Luo,Liu, Bo-Wen,He, Shan,Wang, Zhao Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.6
Full-length cDNA sequences of four novel SPATA4 genes in chimpanzee, cow, chicken and ascidian were identified by bioinformatic analysis using mouse or human SPATA4 cDNA fragment as electronic probe. All these genes have 6 exons and have similar protein molecular weight and do not localize in sex chromosome. The mouse SPATA4 sequence is identified as significantly changed in cryptorchidism, which shares no significant homology with any known protein in swissprot databases except for the homologous genes in various vertebrates. Our searching results showed that all SPATA4 proteins have a putative conserved domain DUF1042. The percentages of putative SPATA4 protein sequence identity ranging from 30% to 99%. The high similarity was also found in 1 kb promoter regions of human, mouse and rat SPATA4 gene. The similarities of the sequences upstream of SPATA4 promoter also have a high proportion. The results of searching SymAtlas (http://symatlas.gnf.org/SymAtlas/) showed that human SPATA4 has a high expression in testis, especially in testis interstitial, leydig cell, seminiferous tubule and germ cell. Mouse SPATA4 was observed exclusively in adult mouse testis and almost no signal was detected in other tissues. The pI values of the protein are negative, ranging from 9.44 to 10.15. The subcellular location of the protein is usually in the nucleus. And the signal peptide possibilities for SPATA4 are always zero. Using the SNPs data in NCBI, we found 33 SNPs in human SPATA4 gene genomic DNA region, with the distribution of 29 SNPs in the introns. CpG island searching gives the data about CpG island, which shows that the regions of the CpG island have a high similarity with each other, though the length of the CpG island is different from each other.This research is a fundamental work in the fields of the bioinformational analysis, and also put forward a new way for the bioinformatic analysis of other genes.
Development of High Spectral Resolution Lidar System for Measuring Aerosol and Cloud
Ming Zhao,Chen-Bo Xie,Zhiqing Zhong,Bang-Xin Wang,Zhenzhu Wang,Pang-Da Dai,Zhen Shang,Min Tan,Dong Liu,Yingjian Wang 한국광학회 2015 Current Optics and Photonics Vol.19 No.6
A high spectral resolution lidar (HSRL) system based on injection-seeded Nd:YAG laser and iodineabsorption filter has been developed for the quantitative measurement of aerosol and cloud. The laserfrequency is stabilized at 80 MHz by a frequency locking system and the absorption line of iodine cellis selected at the 1111 line with 2 GHz width. The observations show that the HSRL can provide verticalprofiles of particle extinction coefficient, backscattering coefficient and lidar ratio for cloud and aerosolup to 12 km altitude, simultaneously. For the measured cases, the lidar ratios are 10~20 sr for cloud,28~37 sr for dust, and 58~70 sr for urban pollution aerosol. It reveals the potential of HSRL to distinguishthe type of aerosol and cloud. Time series measurements are given and demonstrate that the HSRL hasability to continuously observe the aerosol and cloud for day and night