Novel splice isoforms of pig myoneurin and their diverse mRNA expression patterns

Guo, Xiaohong,Li, Meng,Gao, Pengfei,Cao, Guoqing,Cheng, Zhimin,Zhang, Wanfeng,Liu, Jianfeng,Liu, Xiaojun,Li, Bugao Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.10

Objective: The aim of this study was to clone alternative splicing isoforms of pig myoneurin (MYNN), predict the structure and function of coding protein, and study temporal and spatial expression characteristics of each transcript. Methods: Alternative splice isoforms of MYNN were identified using RNA sequencing (RNA-seq) and cloning techniques. Quantitative real-time polymerase chain reaction (qPCR) was employed to detect expression patterns in 11 tissues of Large White (LW) and Mashen (MS) pigs, and to study developmental expression patterns in cerebellum (CE), stomach (ST), and longissimus dorsi (LD). Results: The results showed that MYNN had two alternatively spliced isoforms, MYNN-1 (GenBank accession number: KY470829) and MYNN-2 (GenBank accession number: KY670835). MYNN-1 coding sequence (CDS) is composed of 1,830 bp encoding 609 AA, whereas MYNN-2 CDS is composed of 1,746 bp encoding 581 AA. MYNN-2 was 84 bp less than MYNN-1 and lacked the sixth exon. MYNN-2 was found to have one $C_2H_2$ type zinc finger protein domain less than MYNN-1. Two variants were ubiquitously expressed in all pig tissues, and there were significant differences in expression of different tissues (p<0.05; p<0.01). The expression of MYNN-1 was significantly higher than that of MYNN-2 in almost tissues (p<0.05; p<0.01), which testified that MYNN-1 is the main variant. The expression of two isoforms decreased gradually with increase of age in ST and CE of MS pig, whereas increased gradually in LW pig. In LD, the expression of two isoforms increased first and then decreased with increase of age in MS pig, and decreased gradually in LW pig. Conclusion: Two transcripts of pig MYNN were successfully cloned and MYNN-1 was main variant. MYNN was highly expressed in ST, CE, and LD, and their expression was regular. We speculated that MYNN plays important roles in digestion/absorption and skeletal muscle growth, whereas the specific mechanisms require further elucidation.

Lag Group Consensus for the Second-order Nonlinear Multi-agent Systems via Adaptive Control Approach

Wanli Guo,Wenqiang Luo,Zhimin Zheng 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.8

Second-order nonlinear multi-agent system whose agents could be divided into multiple subgroups isconsidered in this paper. In order to avoid congestion, lag group consensus of the considered system is investigatedvia adaptive protocol. Without constraint on the topology of the system, sufficient conditions for the lag groupconsensus are proposed analytically. Finally, the efficiency of the proposed methodology is illustrated by numericalsimulation.

Cluster-Based Polarized Spectrum Sharing in Channels with Polarization Mode Dispersion

Dongming Li,Zhimin Zeng,Caili Guo,Xiaolin Lin 한국전자통신연구원 2014 ETRI Journal Vol.36 No.3

Polarized spectrum sharing (PSS) exploits the spectrumopportunities in a polarized domain. However, when it comesto wideband environments PSS is impaired by the frequencydependentpolarization mode dispersion (PMD); thus, theeffective throughput of PSS drops. To combat the PMD effect,this work proposes a cluster-based PSS approach to enablePSS on a narrower frequency span. Simulation results showthat the effective throughput of PSS on cluster basisoutperforms that of PSS on bandwidth and subcarrier basis.

Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig

Pengfei Gao,Zhimin Cheng,Meng Li,Ningfang Zhang,Baoyu Le,Wanfeng Zhang,Pengkang Song,Xiaohong Guo,Bugao Li,Guoqing Cao 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.8

Objective: The aim of this study was to select the candidate genes affecting meat quality and preliminarily explore the related molecular mechanisms in the Mashen pig. Methods: The present study explored genetic factors affecting meat quality in the Mashen pig using RNA sequencing (RNA-Seq). We sequenced the transcriptomes of 180-day-old Mashen and Large White pigs using longissimus dorsi to select differentially expressed genes (DEGs). Results: The results indicated that a total of 425 genes were differentially expressed between Mashen and Large White pigs. A gene ontology enrichment analysis revealed that DEGs were mainly enriched for biological processes associated with metabolism and muscle development, while a Kyoto encyclopedia of genes and genomes analysis showed that DEGs mainly participated in signaling pathways associated with amino acid metabolism, fatty acid metabolism, and skeletal muscle differentiation. A MCODE analysis of the protein-protein interaction network indicated that the four identified subsets of genes were mainly associated with translational initiation, skeletal muscle differentiation, amino acid metabolism, and oxidative phosphorylation pathways. Conclusion: Based on the analysis results, we selected glutamic-oxaloacetic transaminase 1, malate dehydrogenase 1, pyruvate dehydrogenase 1, pyruvate dehydrogenase kinase 4, and activator protein-1 as candidate genes affecting meat quality in pigs. A discussion of the related molecular mechanisms is provided to offer a theoretical basis for future studies on the improvement of meat quality in pigs.

Ds9 was isolated encoding as OsHAP3H and its C-terminus was required for interaction with HAP2 and HAP5

Qikai Xing,Zhimin Zheng,Xiangui Zhou,Xujun Chen,Zejian Guo 한국식물학회 2015 Journal of Plant Biology Vol.58 No.1

Heading date is one of the most important agronomic traits in rice. Here, we analyzed an early flowering mutant ds9 under long day (LD) conditions and found that the early flowering phenotype was controlled by a single dominant gene. Mapping the Ds9 revealed that it was located near SSR marker RM5432 on chromosome 8, close to the known heading date-related DTH8/Ghd8 locus. Sequence analysis indicated that Ds9 was allelic to Ghd8, encoding the OsHAP3H subunit of CCAAT-box binding proteins (HAPs), and transformation of ds9 mutant with the OsHAP3H gene from wild type XS11 plants significantly increased the heading date under LD conditions. Yeast two hybrid and three hybrid assays revealed that the C-terminus of OsHAP3H is required for its interactions with OsHAP2 and OsHAP5. The data demonstrated that OsHAP3H plays a critical role as a repressor of photoperiodic flowering in rice.

Correction to: Effects of solvent extraction on the microstructure of bituminous coal‑based graphite

Lipeng Wang,Zongxu Yao,Zhimin Guo,Xiaofeng Shen,Zhiang Li,Zhengqi Zhou,Yuling Wang,Jian‑Guo Yang 한국탄소학회 2022 Carbon Letters Vol.32 No.3

Content-driven Joint Allocation of Communication and Computing Resources in Vehicular Networks

Xu Zhu,Fangfang Liu,Zhimin Zeng,Caili Guo,Jiujiu Chen 한국통신학회 2021 한국통신학회 학술대회논문집 Vol.2021 No.2

With the rapid growth of computer vision applications, a large amount of video data in the Internet of Vehicles scenario are used for content analysis. Tasks based on video content understanding are usually accompanied by huge amount of calculation, which put great pressure on traditional wireless communication resource and Mobile Edge Computing (MEC) server computing resource. Furthermore, existing resource allocation schemes based on Quality of Service (QoS) or Quality of Experience (QoE) may not be the best choice for the purpose of video content understanding. In this paper, we propose a joint resource allocation scheme based on Quality of Content (QoC) to maximize the accuracy of video content understanding. Due to the real-time nature of resource allocation and the variability of the environment in autonomous driving scenarios, we design a Multi-agent Distributed Q-Learning algorithm to solve such multi-constrained nonlinear programming problems. Finally, the simulation results show that our proposed QoC-based joint resource allocation scheme has better video content understanding performance.

Effects of solvent extraction on the microstructure of bituminous coal-based graphite

Wang Lipeng,Yao Zongxu,Guo Zhimin,Shen Xiaofeng,Li Zhiang,Zhou Zhengqi,Wang Yuling,Yang Jian-Guo 한국탄소학회 2022 Carbon Letters Vol.32 No.3

Coal-based graphite has become the main material of emerging industries. The microstructure of coal-based graphite plays an important role in its applications in many fields. In this paper, the effect of carbon disulfide/N-methyl-2-pyrrolidone solvent mixture extraction on the microstructure of bituminous coal-based graphite was systematically studied through preliminary extraction coupled with high-temperature graphitization. The graphitization degree g (75.65%) of the coal residue-based graphite was significantly higher than that of the raw coal-based graphite. The crystallite size La of the coal residue-based graphite was reduced by 47.06% compared with the raw coal-based graphite. The ID/ IG value of the coal residue-based graphite is smaller than that of the raw coal-based graphite. The specific surface area (16.72 m2/g) and total pore volume (0.0567 m3/g) of the coal residue-based graphite are increased in varying degrees compared with the raw coal-based graphite. This study found a carbon source that can be used to prepare coal-based graphite with high graphitization degree. The results are expected to provide a theoretical basis for further clean and efficient utilization of the coal residue resources.

Glutamic-oxaloacetic transaminase 1 regulates adipocyte differentiation by altering nicotinamide adenine dinucleotide phosphate content

Yang Yang,Cheng Zhimin,Zhang Wanfeng,Hei Wei,Lu Chang,Cai Chunbo,Zhao Yan,Gao Pengfei,Guo Xiaohong,Cao Guoqing,Li Bugao 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.2

Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation. Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content. Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p< 0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content. Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism. Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation.Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content.Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p<0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content.Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism.

An Effective and Green Method for the Extraction and Purification of Aglycone Isoflavones from Soybean

Mengfan Wang,Jian Guo,Wei Qi,Rongxin Su,Zhimin He 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.3

In this paper, an effective and green strategy was developed to obtain aglycone isoflavones from soybean through the extraction, hydrolysis, and purification steps. Firstly, a novel ethanol-alkaline extraction method was designed and optimized. The high extraction yield (94.34%)of isoflavones was achieved under the optimal extraction conditions of pH 9.0, 70oC, 60 min, ethanol concentration of 65%, and 1:15 of the solid to liquid ratio. Then, the cellulase (GC-220) was used instead of traditional β-glucosidase to hydrolyze the glycoside isoflavones into aglycone isoflavones which gave an excellent conversion of 95%. Finally, the crude aglycone isoflavones have been purified by an environmental-friendly procedure comprised of ethanol precipitation, ethyl acetate extraction, and water rinse. The final amount of aglycone isoflavones obtained was 1.279 mg for every gram of defatted soybean by this strategy, and the final recovery, and purity of aglycone isoflavones could achieve 80.38 and 87.21%, respectively.