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조건불리지역 재배를 위한 산화스트레스 내성 고구마 품종의 선발
김윤희,박성철,양경실,Zhilin Zhou,Donglan Zhao,Daifu Ma,정재철,이행순,곽상수 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.3
Oxidative stress derived from excess reactive oxygen species (ROS) is a major damaging factor for plants exposed to environmental stresses. Sweetpotato [Ipomoea batatas (L.) Lam] has a relatively broad adaptability to harsh environmental conditions compared to other staple crops. In this study, to select stress-tolerant sweetpotato cultivars for sources of molecular breeding on marginal lands, we evaluated the ion leakage values in 10 different cultivars after treatment of methyl viologen (MV), an ROS-generating nonselective herbicide, to leaf discs. DPPH radical scavenging activity and the contents of total phenolics were also investigated. The ion leakage of each cultivar showed a diverse value, which is well correlated with DPPH radical scavenging activity of each cultivar. DPPH radical scavenging activity also showed a high corelation with the contents of total phenolic contents. Three cultivars of Yanshu 8, Shinhwangmi and Shinzami showed high antioxidant activity. Our results suggest that a simple and efficient DPPH radical scavenging activity would be a suitable method to select potential cultivars with enhanced tolerance to multiple environmental stress. 식물이 환경스트레스에 노출되게 되면 과다한 활성산 소의 축적으로 인한 산화스트레스가 발생한다. 그러므로 재해내성 작물개발을 위한 분자육종의 재료로써 스트레 스 내성 고구마 품종을 선발하기 위해, 본 연구에서는 10 종의 고구마의 잎 disc에 산화스트레스 유도원인 methyl viologen (MV)을 처리하였다. MV 처리 후 각 고구마 품종의 이온전도도는 DPPH radical 소거활성과 높은 상관관계를 나타내었다. 본 연구 에서 낮은 이온전도도 값과 높은 DPPH radical 소거활성 및 전체 페놀함량을 나타낸 Yanshu 8, 신황미, 신자미 세 품종을 산화스트레스 내성 품종으로 선발할 수 있었다. 본 연구의 결과를 통해 DPPH radical 소거활성이 복합재 해내성 작물선발을 위한 좋은 실험방법임을 알 수 있었 으며, 산화스트레스 내성 고구마 품종들이 보인 MV에 대한 내성은 증가된 항산화 활성과 연관된 것임을 알 수 있었다.
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Case Study on the Method of Rock Mass Overburden Consolidation Grouting for Dam Foundation
Jinxi Dou,Xiaolong Yang,Pan Gong,Zhilin Wang,Kexiang Wang,Tiangang Zhou,Weiquan Zhao 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.10
Conventional grouting methods present challenges in achieving effective surface rock grouting and avoiding interference with concrete grouting and construction processes. The proposed approach includes reserving rock overburden, controlling grouting pressure and concentration, using a segmented grouting method, and selecting appropriate materials to develop a key method for consolidation grouting. This method not only overcomes the problems associated with the conventional consolidation grouting method but also effectively protects the thin layer of breccia lava in the Baihetan Dam foundation and solves the problem of unloading and relaxation of columnar jointed basalt caused by exposed consolidation grouting. The results show that the post grouting inspection hole permeability qualification rate is 99.3%, with the proportion of high-speed measuring points of various rock types ranging from 91.8% to 93.3%, and the proportion of low-speed measuring points ranging from 2.0% to 2.9%. The drop rate of the grout take of sequence I and sequence II is between 30% − 60%, except for some dam sections. These results indicate that the proposed method for consolidation grouting meets the design requirements. The findings have significant implications for the design and construction of consolidation grouting in similar projects.
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