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      • KCI등재

        산업용 고구마 개발을 위한 유전자원 현황 및 전망

        목일진,Donglan Zhao,곽상수 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.3

        In many countries including China and U.S., researchers are developing methods to use sweetpotato as raw material for biofuel. We consider the sweetpotato is not only a source of green fuel, it eventually will provide various material including paper, adhesives, biodegradable plastics, and secondary metabolites. Sweetpotato is one of the high efficiency crop because it yields more calories per unit area than either maize or potato, and it requires the shortest growing cycle of the root crops grown in the tropics. Sweetpotato is the most useful crop for the coming starchbased industry era. Sweetpotato genetic resources are collected, characterized, evaluated, and maintained by U.S., China, Japan, and the International Potato Center. New varieties of sweetpotato using the proper genetic resources and molecular breeding will be developed to cope with the global food and energy in 21st century. 세계는 지금 치열한 자원전쟁에 휩싸여 있다. 원유의 가격급등과 품귀현상에 따라 선진국들도 대체 에너지 개발에 열을 올리고 있다. 신재생에너지의 효율성, 경제 성을 제고하기 이전까지는 당분간 바이오연료를 활용하 는 것이 대안이다. 바이오연료로 활용하기 위해서는 몇 가지 고려해야할 것이 있다. 즉 식량생산과의 경합을 최 소화해야 하며 에너지 고정효율이 높은 작물을 이용해 야 한다. 그리고 그 부산물을 활용할 수 있어야 할 것이 다. 고구마는 이러한 조건을 모두 충족하고 있는 우수한 작물로 재평가되고 있다. 따라서 고구마의 유전자원을 수집, 평가하여 목적에 맞는 계통을 선발하고 전통적 육 종 및 형질전환 기법을 활용하여 목적에 맞는 효율성 높 은 품종을 개발하여야 할 것이다.

      • KCI등재후보

        全球价值链嵌入促进中国经济高质量发展了吗?: 基于GVC嵌入对全要素生产率增长效应的实证检验

        许冬兰 ( Donglan Xu ),戴萌萌 ( Mengmeng Dai ),赵倩 ( Qian Zhao ) 성균관대학교 성균중국연구소 2019 중국사회과학논총 Vol.1 No.2

        以全球价值链嵌入(GVC)形式融入国际分工,使中国经济的质量、效率和动力都发生了巨大变化。鉴于此,本文基于增加值视角利用国际投入产出模型测算了中国全球价值链嵌入程度,然后利用动态GMM模型实证考察了GVC嵌入程度对中国制造业全要素生产率(TFP)的影响效应及内在机制,以此揭示GVC嵌入对中国高质量发展的作用效果。研究表明:GVC嵌入程度的加深显著促进了中国TFP的提升, 而这种提升主要是通过价值链对技术进步、规模效率的显著正向影响来实现的,对技术效率的影响却不显著。可知,全球价值链嵌入主要通过技术溢出效应和规模经济效应带动了中国TFP的提升,进而带来推动经济高质量发展的作用效果。此外,研发投入、国际市场参与度、出口密集度等因素也对TFP产生显著的正向影响。 Integrating into the international division of labor in the form of embedding Global Value Chain has resulted in the tremendous change for the quality efficiency and power of China’s economy. Based on the perspective of added value, this research employed the International Input-Output Model to calculate China’s participation level in global value chain (GVC). And then It used the dynamic Generalized method of moments (GMM) model to examine the influence and inherent mechanism of the participation level in GVC on the TFP of manufacturing industry in order to reveal the effect of Global Value Chain embedding on China’s high-quality development. It is found that the increasing of the participation level in GVC has significantly stimulated the growth of China’s TFP, and the participation level in GVC positively affects the TFP through technical progress and scale efficiency instead of technical efficiency. Therefore, the participation level in GVC drives the upgrading of China’s TFP through technology spillover effect and scale economies effect, thus resulting in the development of economy with high quality. In addition, it should be noted that R&D, international market participation, and export’s intensity can also impose significantly positive impact on the TFP.

      • KCI등재

        Expression of lncRNA NEAT1 in endometriosis and its biological functions in ectopic endometrial cells as mediated via miR-124-3p

        Yuan Donglan,Zhu Dandan,Yin Boyu,Ge Hongshan,Zhao Yinling,Huang Aihua,Wang Xiaosu,Cao Xiuhong,Xia Nan,Qian Hua 한국유전학회 2022 Genes & Genomics Vol.44 No.5

        Background: Endometriosis (EM) is a gynecological disease that poses severe health risks to women, although its pathogenesis has yet to be fully elucidated. It has been shown that long non-coding RNAs (lncRNAs) are closely associated with EM initiation and have a role in the development of this disease. Previous studies exploring the expression of the lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) have shown that this lncRNA functions as a tumor promoter in endometrial cancer. However, its exact mechanism of action in EM remains unclear. Objective: This report was designed to illustrate the potential molecular mechanisms of lncRNA NEAT1 on EM. Methods: Endometrial tissues were extracted from EM model rats and patients with EM. Hematoxylin and eosin staining was applied to detect the morphological changes that occurred in rats after construction of the model. Endometrial stromal cells (ESCs) were extracted from either ectopic endometrium (EC) or eutopic endometrium (EU) tissues from patients with EM. LncRNA NEAT1 and miR-124-3p expression in EM tissues and cells were subsequently evaluated by reverse transcription-quantitative (RT-q)PCR analysis. MTT assay, flow cytometric analysis, western blot assay and Transwell assay were then employed to examine the effect of NEAT1 and miR-124-3p on EC-ESC proliferation, apoptosis, migration and invasion, respectively. The targeted relationship between lncRNA NEAT1 and miR-124-3p was subsequently confirmed by dual-luciferase and co-transfection assays. Results: MiR-124-3p was identified as a target of NEAT1, and could be negatively regulated by NEAT1 in EC-ESCs. The expression level of NEAT1 was evidently increased, whereas that of miR-124-3p was decreased, in the EM in vivo model, EM tissues and EC-ESCs from patients with EM. The loss-of-function assays further established that silencing of NEAT1 could inhibit EC-ESC proliferation, migration, and invasion, but it led to the promotion of apoptosis via targeting miR-124-3p. Conclusions: NEAT1 is significantly upregulated in EM, promoting malignant behavior in EM through targeting miR-124-3p expression.

      • KCI등재

        조건불리지역 재배를 위한 산화스트레스 내성 고구마 품종의 선발

        김윤희,박성철,양경실,Zhilin Zhou,Donglan Zhao,Daifu Ma,정재철,이행순,곽상수 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.3

        Oxidative stress derived from excess reactive oxygen species (ROS) is a major damaging factor for plants exposed to environmental stresses. Sweetpotato [Ipomoea batatas (L.) Lam] has a relatively broad adaptability to harsh environmental conditions compared to other staple crops. In this study, to select stress-tolerant sweetpotato cultivars for sources of molecular breeding on marginal lands, we evaluated the ion leakage values in 10 different cultivars after treatment of methyl viologen (MV), an ROS-generating nonselective herbicide, to leaf discs. DPPH radical scavenging activity and the contents of total phenolics were also investigated. The ion leakage of each cultivar showed a diverse value, which is well correlated with DPPH radical scavenging activity of each cultivar. DPPH radical scavenging activity also showed a high corelation with the contents of total phenolic contents. Three cultivars of Yanshu 8, Shinhwangmi and Shinzami showed high antioxidant activity. Our results suggest that a simple and efficient DPPH radical scavenging activity would be a suitable method to select potential cultivars with enhanced tolerance to multiple environmental stress. 식물이 환경스트레스에 노출되게 되면 과다한 활성산 소의 축적으로 인한 산화스트레스가 발생한다. 그러므로 재해내성 작물개발을 위한 분자육종의 재료로써 스트레 스 내성 고구마 품종을 선발하기 위해, 본 연구에서는 10 종의 고구마의 잎 disc에 산화스트레스 유도원인 methyl viologen (MV)을 처리하였다. MV 처리 후 각 고구마 품종의 이온전도도는 DPPH radical 소거활성과 높은 상관관계를 나타내었다. 본 연구 에서 낮은 이온전도도 값과 높은 DPPH radical 소거활성 및 전체 페놀함량을 나타낸 Yanshu 8, 신황미, 신자미 세 품종을 산화스트레스 내성 품종으로 선발할 수 있었다. 본 연구의 결과를 통해 DPPH radical 소거활성이 복합재 해내성 작물선발을 위한 좋은 실험방법임을 알 수 있었 으며, 산화스트레스 내성 고구마 품종들이 보인 MV에 대한 내성은 증가된 항산화 활성과 연관된 것임을 알 수 있었다.

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