Effects of mouthwashes on cariogenic properties of Streptococcus mutans

Zhibin Liu,Ki-Rim Kim 한국실험동물학회 2023 한국실험동물학회 학술발표대회 논문집 Vol.2023 No.2

Ethanol Production by In situ Xylose Isomerization Using Recombinant Escherichia coli and Fermentation Using Conventional Saccharomyces cerevisiae

Zhibin Liu,Jinchuan Wu 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.5

Xylose isomerase from Geobacillus kaustophilus HTA426 was functionally expressed in Escherichia coli BL21 (DE3) and the recombinant E. coli cells were used together with conventional Saccharomyces cerevisiae to produce ethanol from xylose by simultaneous xylose isomerisation and fermentation. When recombinant E. coli cells were used as the source of xylose isomerase, a significant amount of ethanol was produced from xylose,whereas the control without recombinant E. coli cells did not produce any detectable amount of ethanol from xylose. Ethanol production was increased by 38% by feeding more recombinant E. coli at 48 h compared to adding recombinant E. coli only in the beginning, resulting in more ethanol production than P. stipitis CBS6054 under the same conditions. The xylitol accumulation by the in situ process was only 57% of that produced by the P. stipitis CBS6054. Xylose isomerase from Geobacillus kaustophilus HTA426 was functionally expressed in Escherichia coli BL21 (DE3) and the recombinant E. coli cells were used together with conventional Saccharomyces cerevisiae to produce ethanol from xylose by simultaneous xylose isomerisation and fermentation. When recombinant E. coli cells were used as the source of xylose isomerase, a significant amount of ethanol was produced from xylose,whereas the control without recombinant E. coli cells did not produce any detectable amount of ethanol from xylose. Ethanol production was increased by 38% by feeding more recombinant E. coli at 48 h compared to adding recombinant E. coli only in the beginning, resulting in more ethanol production than P. stipitis CBS6054 under the same conditions. The xylitol accumulation by the in situ process was only 57% of that produced by the P. stipitis CBS6054.

Optimized Distributed Fusion Filtering for Uncertain Nonlinear Systems With Missing Measurements: Algorithm Design and Boundedness Analysis

Zhibin Hu,Jun Hu,Junhua Du,Hongjian Liu,Jun Qi 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.10

This paper investigates the optimized distributed fusion filtering (DFF) problem for a class of nonlinear discrete time-varying stochastic systems with randomly occurring uncertainty (ROU) and missing measurements (MMs). The stochastic nonlinearity is depicted in terms of statistical means. The phenomena of the ROU and MMs are considered during the modelling of state equation and measurement output respectively, which are characterized by Bernoulli distributed random variables. In order to deal with the effect of the parameter uncertainty, the method that the local estimation error covariances and cross-covariances from all estimators at every sample time are replaced by their upper bounds is adopted. Moreover, the minimum upper bounds for each filtering error covariance (FEC) are obtained by designing the corresponding filter gains. Based on the local filters, a new robust DFF algorithm is developed via the matrix-weighted fusion method. In addition, a sufficient condition concerning on the performance analysis of the developed algorithm is given, which can show that the boundedness of the upper bound for each FEC is guaranteed. Finally, a numerical example is provided to manifest the usefulness of the developed distributed fusion algorithm.

miR-143 inhibits proliferation and induces apoptosis of mammary epithelial cells in dairy goat

Zhibin Ji,Guizhi Wang,Lei Hou,Zhaohua Liu,Jianmin Wang,Tianle Chao 한국통합생물학회 2016 Animal cells and systems Vol.20 No.2

MicroRNAs are a class of post-transcriptional regulators of gene expression in multicellular organisms, which play important roles in cell fate, organ morphogenesis and carcinogenesis. In the present study, we demonstrated the critical roles of miR-143 on mammary epithelial cells of dairy goat. The test results of cell count, methylthiazolyldiphenyl-tetrazolium bromide, Hoechst33342/PI (propidium iodide) and flow cytometry showed miR-143-induced G0/G1 phase arrest, inhibited proliferation and promoted apoptosis of mammary epithelial cells; the qRT-PCR test of marker genes related to cell proliferation and apoptosis, BAX and BCL-2, supported the same conclusions. Our study presents evidence that miR-143 is an important post-transcription regulator involving in mammary cells survival, and it may have a value function in mammary gland development, lactation or involution.

Functional analysis of prv-miR-LLT11a encoded by pseudorabies virus

Huimin Liu,Li Yang,Zhibin Shi,Ruiqi Lv,Xia Yang,Chuanqing Wang,Lu Chen,Hongtao Chang 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.6

Viral-encoded microRNAs (miRNAs) play have vital roles in the regulations of virus replications and host immune responses. The results of previous studies have indicated that miRNA clusters are involved in the replication and virulence of the pseudorabies virus (PRV), which may potentially lead to the immune escape or facilitation of PRV replications. This study's previous research revealed that the prv-mirmiR-LLT11a was differentially expressed during PRV infections. The present study's results have demonstrated that the prv-miR-LLT11a could significantly inhibit PRV replications. It was further determined that SLA-1 was the target gene of the prv-miR-LLT11a, and simultaneously, thate overexpression of prv-miR-LLT11a could down-regulate the mRNA and protein levels of SLA-1 in a dose-independent manner. Furthermore, the present study also found observed that the prv-miR-LLT11a canhad also down-regulated the TAP1 expressions. Our findings provide a better understanding of the molecular mechanism involved in on the effects of prv-miR-LLT11a on SLA-1 and TAP1, as well as and its involvement in a potential immune system evasion of PRV.

Microbiota Associated with the Starter Cultures and Brewing Process of Traditional Hong Qu Glutinous Rice Wine

박관화,Zhibin Liu,박천석,Li Ni 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.3

Hong Qu glutinous rice wine (produced mainly in Fujian province, China) is a traditional alcoholic beverage, which is prepared by fermenting cooked rice using a starter containing Monascus purpureus. In this review, the microbial diversity of fermentation starters from Fujian province, including fungi, bacteria, and yeast, is analyzed in comparison with those of “nuruk” (a traditional starter for making alcoholic beverages in Korea). The bacterial organization of Hong Qu starters was vastly variable in species composition and dominated by Bacillus sp. Lactic acid bacteria were also found in some starters. In case of fungi, Monascus sp. was dominant, whereas non-Saccharomyces yeast such as Saccharomycopsis fibuligera was detected. The microorganisms found in the nuruk starter are, in general, not significantly diverse compared with those found in the Hong Qu starter, with the exception of Monascus sp.; however, Hong Qu and nuruk both contain their own unique microbiota, which are quite diverse from each other.

Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats

Ji, Zhibin,Wang, Guizhi,Zhang, Chunlan,Xie, Zhijing,Liu, Zhaohua,Wang, Jianmin Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3

MicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goats (Capra hircus) were constructed and sequenced, individually. Through Solexa high-throughput sequencing and bioinformatics analysis, we obtained 50 presumptive novel miRNAs candidates, and 55,448 putative target genes were predicted. GO annotations and KEGG pathway analyses showed the majority of target genes were involved in various biological processes and metabolic pathways. Our results discovered more information about the regulation network between miRNAs and mRNAs and paved a foundation for the molecular genetics of mammary gland development in goats.

Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers

Sun, Zhibin,Fan, Jiadong,Li, Haoyuan,Liu, Huajie,Nam, Daewoong,Kim, Chan,Kim, Yoonhee,Han, Yubo,Zhang, Jianhua,Yao, Shengkun,Park, Jaehyun,Kim, Sunam,Tono, Kensuke,Yabashi, Makina,Ishikawa, Tetsuya,So American Chemical Society 2018 ACS NANO Vol.12 No.8

<P>It has been proposed that the radiation damage to biological particles and soft condensed matter can be overcome by ultrafast and ultraintense X-ray free-electron lasers (FELs) with short pulse durations. The successful demonstration of the “diffraction-before-destruction” concept has made single-shot diffraction imaging a promising tool to achieve high resolutions under the native states of samples. However, the resolution is still limited because of the low signal-to-noise ratio, especially for biological specimens such as cells, viruses, and macromolecular particles. Here, we present a demonstration single-shot diffraction imaging experiment of DNA-based structures at SPring-8 Angstrom Compact Free Electron Laser (SACLA), Japan. Through quantitative analysis of the reconstructed images, the scattering abilities of gold and DNA were demonstrated. Suggestions for extracting valid DNA signals from noisy diffraction patterns were also explained and outlined. To sketch out the necessary experimental conditions for the 3D imaging of DNA origami or DNA macromolecular particles, we carried out numerical simulations with practical detector noise and experimental geometry using the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory, USA. The simulated results demonstrate that it is possible to capture images of DNA-based structures at high resolutions with the technique development of current and next-generation X-ray FEL facilities.</P> [FIG OMISSION]</BR>

Fatigue study on additional cutout between U shaped rib and floorbeam in orthotropic bridge deck

Xiaochen Ju,Zhibin Zeng,Xinxin Zhao,Xiaoguang Liu 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.28 No.3

The field around additional cutout of the floor beam web in orthotropic bridge deck was subjected to high stress concentration, especially the weld toe between floor beam and U shaped rib and the free edge of the additional cutout. Based on different considerations, different geometrical parameters of additional cutout were proposed in European, American and Japanese specifications, and there remained remarkable differences among them. In this study, considering influence of out-ofplane deformation of floor beam web and U shaped rib, parameter analysis for additional cutout under typical load cases was performed by fine finite element method. The influence of additional cutout shape and height to the stress distribution around the additional cutout were investigated and analyzed. Meanwhile, the static and fatigue test on this structure details was carried out. The stress distribution was consistent with the finite element analysis results. The fatigue property for additional cutout height of 95mm was slightly better than that of 61.5 mm.

Evaluating and predicting net energy value of wheat and wheat bran for broiler chickens

Ning Ran,Cheng Zichen,Liu Xingbo,Ban Zhibin,Guo Yuming,Nie Wei 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.11

Objective: It is crucial to accurately determine the net energy (NE) values of feed ingredients because the NE system is expected to be applied to the formulation of broilers feed. The NE values of 5 wheat and 5 wheat brans were determined in 12-to 14-day old Arbor Acres (AA) broilers with substitution method and indirect calorimetry method. Methods: A total of 12 diets, including 2 reference diets (REF) and 10 test diets (5 wheat diets and 5 wheat bran diets) containing 30% of test ingredients, were randomly fed to 864 male AA birds with 6 replicates of 12 birds per treatment. These birds were used to determine metabolizable energy (ME) (8 birds per replicate) in the chicken house and NE (4 birds per replicate) in the chamber respectively at the same time. After a 4-d dietary and environment adaptation period, growth performance, energy values, energy balance and energy utilization were measured during the following 3 d. Multiple linear regression analyses were further performed to generate prediction equations for NE values based on the chemical components and ME values. The NE prediction equation were also validated on another wheat diet and another wheat bran diet with high correlation (r = 0.98, r = 0.75). Results: The NE values of 5 wheat and 5 wheat bran samples are 9.34, 10.02, 10.27, 11.33, and 10.49 MJ/kg, and 5.37, 5.17, 4.87, 5.06, and 4.88 MJ/kg DM, respectively. The equation with the best fit were NE = 1.968AME–0.411×ADF–14.227 (for wheat) and NE = –0.382×CF –0.362×CP–0.244×ADF+20.870 (for wheat bran). Conclusion: The mean NE values of wheat and wheat bran are 10.29 and 5.07 MJ/kg DM in AA broilers. The NE values of ingredients could be predicted by their chemical composition and energy value with good fitness. Objective: It is crucial to accurately determine the net energy (NE) values of feed ingredients because the NE system is expected to be applied to the formulation of broilers feed. The NE values of 5 wheat and 5 wheat brans were determined in 12-to 14-day old Arbor Acres (AA) broilers with substitution method and indirect calorimetry method.Methods: A total of 12 diets, including 2 reference diets (REF) and 10 test diets (5 wheat diets and 5 wheat bran diets) containing 30% of test ingredients, were randomly fed to 864 male AA birds with 6 replicates of 12 birds per treatment. These birds were used to determine metabolizable energy (ME) (8 birds per replicate) in the chicken house and NE (4 birds per replicate) in the chamber respectively at the same time. After a 4-d dietary and environment adaptation period, growth performance, energy values, energy balance and energy utilization were measured during the following 3 d. Multiple linear regression analyses were further performed to generate prediction equations for NE values based on the chemical components and ME values. The NE prediction equation were also validated on another wheat diet and another wheat bran diet with high correlation (r = 0.98, r = 0.75).Results: The NE values of 5 wheat and 5 wheat bran samples are 9.34, 10.02, 10.27, 11.33, and 10.49 MJ/kg, and 5.37, 5.17, 4.87, 5.06, and 4.88 MJ/kg DM, respectively. The equation with the best fit were NE = 1.968AME–0.411×ADF–14.227 (for wheat) and NE = –0.382×CF–0.362×CP–0.244×ADF+20.870 (for wheat bran).Conclusion: The mean NE values of wheat and wheat bran are 10.29 and 5.07 MJ/kg DM in AA broilers. The NE values of ingredients could be predicted by their chemical composition and energy value with good fitness.