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Zhibin Liu,Jinchuan Wu 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.5
Xylose isomerase from Geobacillus kaustophilus HTA426 was functionally expressed in Escherichia coli BL21 (DE3) and the recombinant E. coli cells were used together with conventional Saccharomyces cerevisiae to produce ethanol from xylose by simultaneous xylose isomerisation and fermentation. When recombinant E. coli cells were used as the source of xylose isomerase, a significant amount of ethanol was produced from xylose,whereas the control without recombinant E. coli cells did not produce any detectable amount of ethanol from xylose. Ethanol production was increased by 38% by feeding more recombinant E. coli at 48 h compared to adding recombinant E. coli only in the beginning, resulting in more ethanol production than P. stipitis CBS6054 under the same conditions. The xylitol accumulation by the in situ process was only 57% of that produced by the P. stipitis CBS6054. Xylose isomerase from Geobacillus kaustophilus HTA426 was functionally expressed in Escherichia coli BL21 (DE3) and the recombinant E. coli cells were used together with conventional Saccharomyces cerevisiae to produce ethanol from xylose by simultaneous xylose isomerisation and fermentation. When recombinant E. coli cells were used as the source of xylose isomerase, a significant amount of ethanol was produced from xylose,whereas the control without recombinant E. coli cells did not produce any detectable amount of ethanol from xylose. Ethanol production was increased by 38% by feeding more recombinant E. coli at 48 h compared to adding recombinant E. coli only in the beginning, resulting in more ethanol production than P. stipitis CBS6054 under the same conditions. The xylitol accumulation by the in situ process was only 57% of that produced by the P. stipitis CBS6054.
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Chow, Yvonne,Li, Ruijiang,Wu, Jinchuan,Puah, Sze Min,New, Shu Wei,Chia, Wei Qiang,Lie, Felicia,Rahman, Talukder Md Mahabubur,Choi, Won-Jae Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.4
The production of semi-synthetic beta-Iactam antibiotics such as Amoxicillin may be performed enzymatically using penicillin acylase under mild conditions. However, the thermodynamically favored hydrolysis of the antibiotic product and the acyl donor substrate needs to be minimized to use the kinetically controlled route. The addition of cosolvents such as ethylene glycol and methanol (the two best solvents identified so far for semi-synthetic beta-lactam antibiotics) can achieve this to some degree, but these additives also produce enzyme inhibition and deactivation. In this study, we compared ethylene glycol and methanol under various substrate conditions. Methanol gave a better synthesis to hydrolysis ratio, although its deactivating effects adversely affected production at lower cosolvent concentrations than ethylene glycol. This effect and its dependence on substrate concentration was further modeled and optimized. A few targets of optimization such as Amoxicillin level, the synthesis to hydrolysis ratio, or a combination, were employed. While maximum levels of Amoxicillin synthesis were achievable only at high substrate concentrations, improvements derived from cosolvents were most significant at low substrate concentrations.
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Characteristics of Copper-Clad Aluminum Rods Prepared by Horizontal Continuous Casting
Yubo Zhang,Ying Fu,Jinchuan Jie,Li Wu,Kateryna Svynarenko,Qingtao Guo,Tingju Li,Tongmin Wang 대한금속·재료학회 2017 METALS AND MATERIALS International Vol.23 No.6
An innovative horizontal continuous casting method was developed and successfully used to prepare copper-cladaluminum (CCA) rods with a diameter of 85 mm and a sheath thickness of 16 mm. The solidification structure andelement distribution near the interface of the CCA ingots were investigated by means of a scanning electron microscope,an energy dispersive spectrometer, and an electron probe X-ray microanalyzer. The results showed that theproposed process can lead to a good metallurgical bond between Cu and Al. The interface between Cu and Al wasa multilayered structure with a thickness of 200 μm, consisting of Cu9Al4, CuAl2, α-Al/CuAl2 eutectic, and α-Al +α-Al/CuAl2 eutectic layers from the Cu side to the Al side. The mean tensile-shear strength of the CCA sample was45 MPa, which fulfills the requirements for the further extrusion process. The bonding and diffusion mechanismsare also discussed in this paper.
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Yvonne Chow,Ruijiang Li,Jinchuan Wu,Sze Min Puah,Shu Wei New,Wei Qiang Chia,Felicia Lie,Talukder Md Mahabubur Rahman,Won Jae Choi 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.4
The production of semi-synthetic beta-lactam antibiotics such as Amoxicillin may be performed enzymatically using penicillin acylase under mild conditions. However, the thermodynamically favored hydrolysis of the antibiotic product and the acyl donor substrate needs to be minimized to use the kinetically controlled route. The addition of cosolvents such as ethylene glycol and methanol (the two best solvents identified so far for semi-synthetic beta-lactam antibiotics) can achieve this to some degree, but these additives also produce enzyme inhibition and deactivation. In this study, we compared ethylene glycol and methanol under various substrate conditions. Methanol gave a better synthesis to hydrolysis ratio, although its deactivating effects adversely affected production at lower cosolvent concentrations than ethylene glycol. This effect and its dependence on substrate concentration was further modeled and optimized. A few targets of optimization such as Amoxicillin level, the synthesis to hydrolysis ratio, or a combination, were employed. While maximum levels of Amoxicillin synthesis were achievable only at high substrate concentrations, improvements derived from cosolvents were most significant at low substrate concentrations.
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