RBPJ contributes to acquired docetaxel resistance in prostate cancer cells

Li Xue,Zhenlong Wang,Hecheng Li,Zhaolun Li,Qi Chen,Peng Zhang,Haiwen Chen,Ziming Wang,Tie Chong,T. Chong 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.3

Our previous work has shown that depletion of recombination signal-binding protein J (RBPJ) results in reduced cell growth in prostate cancer cells. In this study, we aimed to investigate the function of RBPJ in the chemoresistance of prostate cancer. The expression of RBPJ was quantified in docetaxel-resistant and parental prostate cancer cells. Loss- and gainof- function experiments were conducted to explore the regulatory role of RBPJ in prostate cancer sensitivity to docetaxel. The pro-apoptotic effect of RBPJ silencing was checked in docetaxel-resistant prostate cancer cells. We found that docetaxel-resistant PC3-DR and DU145- DR cells expressed 3-5-fold high levels of RBPJ than parental PC3 and DU145 cells. Short hairpin RNAmediated knockdown of RBPJ inhibited cell proliferation and colony formation and reversed docetaxel resistance in docetaxel-resistant prostate cancer cells. In contrast, overexpression of RBPJ promoted cell growth, colony formation, and docetaxel resistance in parental prostate cancer cells. Downregulation of RBPJ induced apoptosis in docetaxel-resistant cells, which was accompanied by enhanced cleavage of caspase-3. In addition, RBPJ silencing or overexpression markedly modulated the expression of the Bcl-2 family members including Bcl-2, Bcl-xL, Mcl-1, Bax, and Bak. Altogether, RBPJ contributes to acquisition of docetaxel resistance in prostate cancer cells and may thus represent a potential target for overcoming chemotherapeutic resistance in this malignancy.

Isolation and Characterization of Cold- Adapted PGPB and Their Effect on Plant Growth Promotion

( Mingyuan Li ),( Jilian Wang ),( Tuo Yao ),( Zhenlong Wang ),( Huirong Zhang ),( Changning Li ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.9

Cold-adapted plant growth-promoting bacteria (PGPB) with multiple functions are an important resource for microbial fertilizers with low-temperature application. In this study, culturable coldadapted PGPB strains with nitrogen fixation and phosphorus solubilization abilities were isolated. They were screened from root and rhizosphere of four dominant grass species in nondegraded alpine grasslands of the Qilian Mountains, China. Their other growth-promoting characteristics, including secretion of indole-3-acetic acid (IAA), production of siderophores and ACC deaminase, and antifungal activity, were further studied by qualitative and quantitative methods. In addition, whether the PGPB strains could still exert plant growth-promoting activity at 4°C was verified. The results showed that 67 isolates could maintain one or more growth-promoting traits at 4°C, and these isolates were defined as cold-adapted PGPB. They were divided into 8 genera by 16S rRNA gene sequencing and phylogenetic analysis, of which Pseudomonas (64.2%) and Serratia (13.4%) were the common dominant genera, and a few specific genera varied among the plant species. A test-tube culture showed that inoculation of Elymus nutans seedlings with cold-adapted PGPB possessing different functional characteristics had a significant growth-promoting effect under controlled low-temperature conditions, including the development of the roots and aboveground parts. Pearson correlation analysis revealed that different growth-promoting characteristics made different contributions to the development of the roots and aboveground parts. These cold-adapted PGPB can be used as excellent strain resources suitable for the near-natural restoration of degraded alpine grasslands or agriculture stock production in cold areas.

EFFECTS OF COOPERATIVE VEHICLE INFRASTRUCTURE SYSTEM ON DRIVER’S VISUAL AND DRIVING PERFORMANCE BASED ON COGNITION PROCESS

Xuewei Li,Zhenlong Li,Xiaohua Zhao,Jian Rong,Yunlong Zhang 한국자동차공학회 2022 International journal of automotive technology Vol.23 No.5

This study explores the influence of cooperative vehicle infrastructure system (CVIS) on the driver’s visual and driving performance. Taking the work zone as an example, a driving simulation experiment involving 37 drivers has been conducted to collect driver’s eye movement and vehicle running data in the same scenario with and without CVIS information respectively. Next, the nonparametric test and grey relational analysis (GRA) have been used to compare the specific performance and to analyze the correlations among the multi-stage of driver’s information process. The results present that CVIS information has the potential to alleviate the driver’s tension and the difficulty of information perception. Meanwhile, the driver tends to search visual information more actively and complete the lane changing behavior earlier. Further analysis indicates that CVIS information can reduce the influence of mental workload on lane-changing decisions, and enhance the correlation between visual information processing and lane-changing decisions, as well as the effect of decision-making timing on the running state. Therefore, drivers’ speed control ability was improved and the traffic flow was smoother. The findings give an insight into the influence mechanism of the cooperative information on driving performance and provide a direction for a comprehensive assessment of CVIS based on human factors.

A New Quinoline-Based Acylhydrazone for Highly Selective Fluorescence Recognition of Cu(II) and Sulfide in Aqueous Solution

Li-Jun Tang,Pei Zhou,Zhikai Qi,Zhenlong Huang,Jia Zhao,Mingjun Cai 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.8

A new quinoline-based acylhydrazone (1) has been synthesized and applied as a fluorescent probe. Probe 1 exhibits high selectivity and sensitivity to Cu2+ with fluorescence “ON-OFF” behavior in HEPES buffered (1‰ DMSO, HEPES 20 mM, pH = 7.4) solution. The on-site generated 1-Cu2+ complex displays excellent selectivity to sulfide ions with fluorescence “OFF-ON” performance through copper displacement approach.

New Application of 2-(4-N-Phenyl-3-thiosemicarbazone)-8-hydroxyquinoline as a Sensor for Relay Recognition of Cu2+ and Sulfide in Aqueous Solution

Li-Jun Tang,Pei Zhou,Zhenlong Huang,Jia Zhao,Mingjun Cai 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.10

Fluorescent and colorimetric recognition properties of 2-(4-N-phenyl-3-thiosemicarbazone)-8-hydroxyquinoline (1) in buffered aqueous solution (1% DMSO, HEPES 20 mM, pH = 7.4) have been examined. Sensor 1 displays highly selective and sensitive recognition to Cu2+ with fluorescence “ON-OFF” performance. The in situ formed 1-Cu2+ complex exhibits an excellent selectivity toward sulfide ions with fluorescence “OFF-ON” behavior via Cu2+ displacement approach. Thus, relay recognition of Cu2+ and sulfide by a known molecule 1 has been achieved.

A Quinoline-thiooxorhodamine Conjugate for Fluorescent Hg2+ Recognition in Aqueous Media and Living Cells

Li-Jun Tang,Xin Wen,Xin Dai,Di Wu,Zhenlong Huang 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.11

A quinoline-thiooxorhodamine conjugate fluorescent sensor (1) has been synthesized. Sensor 1 exhibits high selectivity and sensitivity to Hg2+ in H2O/DMSO (95/5, v/v, HEPES 20 mM, pH = 7.4) solution with fluorescence detection. Other tested metal ions do not induce any significant fluorescence intensity changes. Sensor 1 interacts with Hg2+ through a 1:1 binding stoichiometry with a good anti-inference ability. In addition, fluorescent imaging of Hg2+ in Hela cells is also successfully demonstrated.

CFD Simulation of Effect of Vortex Ring for Squid Jet Propulsion And Expeiments on a Bionic Jet Propulsor

Li Jian,Zhao Jianing,Wang Zhenlong 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.5

Using jet propulsion, squid can swim at high speed or at low speed with good maneuverability, which makes them quiet valuable to be studied for biomimetic purposes. Vortex rings usually occur in the highly-unsteady jet flow in squid, and they play quite important roles in the jet propulsion of squid. This paper tries to investigate the squid jet structure by computational fluid dynamics (CFD) analysis. A simplified squid body model was established. The mantle motion during jet locomotion was explicitly included into the simulations by using a deforming mesh. By solving the 2D-axisymmetric, incompressible, laminar, unsteady Navier.Stokes equations, different vortex evolution behaviors were observed depending on different mantle contraction velocities and nozzle diameters. An important parameter, the formation number of the vortex rings, L/D, which decide the propulsive efficiency of jet propulsion directly, was also discussed in this paper. The numerical results show that adult squid propel themselves by long jet flows with a large formation number, L/D. The results also prove that smaller squid have larger relative funnel diameter. Interaction of vortex rings was simulated in two jet process, which might interpret squid increase their contraction frequencies with elevated swimming speed. To validate the force generated in the simulation, a bionc squid mantel jet propulsor is investigated and tested.

Active Disturbance Rejection Control for Fluidized Bed Combustor

Zhenlong Wu,Donghai Li,Yali Xue,Lingmei Wang,Jing Wang 제어로봇시스템학회 2016 제어로봇시스템학회 국제학술대회 논문집 Vol.2016 No.10

Active disturbance rejection control (ADRC) is applied in fluidized bed combustor (FBC) facing some control difficulty. By analyzing the difficulties, ADRC controllers are designed for the power loop and the bed temperature loop of FBC system. The simulation demonstrates both loops with ADRC have a better tracking speed and a stronger robustness, the power loop can inhibit the input and output disturbance quickly and the temperature loop has a slight advantage in disturbance rejection. Besides, manipulated variables is analyzed by the phase diagrams.

The Disturbance Rejection Design Based on Physical Feedforward for Solid Oxide Fuel Cell

Zhenlong Wu,Wangying Shi,Donghai Li,Ting He,Yali Xue,Minfang Han,Song Zheng 제어로봇시스템학회 2017 제어로봇시스템학회 국제학술대회 논문집 Vol.2017 No.10

The output voltage control of the Solid Oxide Fuel Cell (SOFC) is challenging due to the strong nonlinearity, modelling uncertainty, the dramatic change of the external current load and constraints on the amplitudes and change rates of the actuator. Besides, the desired range of the fuel utilization is another difficult for the control strategy. In this paper, Active disturbance rejection control (ADRC) is proposed to handle the nonlinearity, uncertainty and the external disturbance, and physical feedforward is designed to speed up the response of the whole system and keep the fuel utilization in the desired range. Instead of the SOFC model, the design of physical feedforward is based on the definition of fuel utilization which is featured by simplicity, generality and effectiveness. Simulation results show that the proposed control strategy has smaller variation range of the fuel utilization, shorter time of the changing process of the output voltage and better robustness than others control strategies.

Frequency-domain Analysis of A Modified Active Disturbance Rejection Control With Application to Superheated Steam Temperature Control

Zhenlong Wu,Fan Zhang,Gengjin Shi,Ting He,Donghai Li,YangQuan Chen 제어로봇시스템학회 2019 제어로봇시스템학회 국제학술대회 논문집 Vol.2019 No.10

Superheated steam temperature (SST) systems face many control difficulties caused by unknown disturbances and high order dynamics. This is becoming more and more severe due to the rapid growing of intermittent renewable generation. To improve the tracking and disturbance rejection performance of SST systems, a modified active disturbance rejection control (ADRC) is proposed. Based on its equivalent structure, this paper develops the frequency-domain analysis of the modified ADRC to present the influence of parameters on the control performance. Then the modified ADRC based on the frequency-domain analysis is applied to the SST control to verify the superiority of the modified ADRC. Based on the results of the simulation and the field application on a power plant simulator, the modified ADRC can obtain the best tracking performance with a small overshoot and short settling time compared with other comparative controllers. Besides, the modified ADRC has the smallest fluctuation range of SST under the input disturbance and load varying conditions. The superiority of the modified ADRC ensures the satisfactory control performance and shows a great potential in industrial applications.