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A Simple Benzimidazole Based Fluorescent Sensor for Ratiometric Recognition of Zn2+ in Water
Keli Zhong,Mingjun Cai,Shuhua Hou,Yanjiang Bian,Lijun Tang 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.2
A phenylbenzimidazole derivatized sensor (L) that behaves as a ratiometric fluorescent receptor for Zn 2+ in water has been described. In HEPES buffer at pH 7.4, sensor L displays a weak fluorescence emission band at 367 nm. Upon addition of Zn 2+ , the emission intensity at 367 nm is decreased, concomitantly, a new emission band centered at 426 nm is developed, thus facilitates a ratiometric Zn 2+ sensing behavior. Sensor L binds Zn 2+ through a 1:1 binding stoichiometry with high selectivity over other metal cations. Sensor L displays a linear response to Zn 2+ concentration from 0 to 6.0 × 10 -5 M, sensor L also exhibits high sensitivity to Zn 2+ with a detection limit of 3.31 × 10 -7 M.
Liao Yi,Cai Qi,He Shaopeng,Wang Mingjun,Xiao Hongguang,Gong Zili,Wang Cong,Jia Zhen,Feng Tangtao,Qiu Suizheng 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.4
Shell-and-tube heat exchanger (STHX) is widely used by virtue of its simple structure and high reliability, especially in a space-constrained surface ship. For the STHX of the surface ship, roll, pitch and other motion of the ship will affect the heat transfer performance, resistance characteristics and structural strength of the heat exchanger. Therefore, it is urgent to carry out numerical simulation research on three-dimensional thermal hydraulic characteristics of surface ship STHX under the marine conditions. In this paper, the numerical simulation of marine shell and tube heat exchanger of surface ship was carried out using the porous media model. Firstly, the mathematical physical model and numerical method are validated based on the experimental data of a marine engine cooling water shell and tube heat exchanger. The simulation results are in good agreement with the experimental results. The prediction errors of pressure drop and heat transfer are less than 10% and 1% respectively. The effect of marine conditions on the heat transfer characteristics of the heat exchanger is investigated by introducing the additional force model of marine condition to evaluate the effect of different motion parameters on the heat transfer performance of the heat exchanger. This study could provide a reference for the optimization of marine heat exchanger design.
Atomic Force Microscopy of Asymmetric Membranes from Turtle Erythrocytes
Tian, Yongmei,Cai, Mingjun,Xu, Haijiao,Ding, Bohua,Hao, Xian,Jiang, Junguang,Sun, Yingchun,Wang, Hongda Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.8
The cell membrane provides critical cellular functions that rely on its elaborate structure and organization. The structure of turtle membranes is an important part of an ongoing study of erythrocyte membranes. Using a combination of atomic force microscopy and single-molecule force spectroscopy, we characterized the turtle erythrocyte membrane structure with molecular resolution in a quasi-native state. High-resolution images both leaflets of turtle erythrocyte membranes revealed a smooth outer membrane leaflet and a protein covered inner membrane leaflet. This asymmetry was verified by single-molecule force spectroscopy, which detects numerous exposed amino groups of membrane proteins in the inner membrane leaflet but much fewer in the outer leaflet. The asymmetric membrane structure of turtle erythrocytes is consistent with the semi-mosaic model of human, chicken and fish erythrocyte membrane structure, making the semi-mosaic model more widely applicable. From the perspective of biological evolution, this result may support the universality of the semi-mosaic model.
Atomic Force Microscopy of Asymmetric Membranes from Turtle Erythrocytes
Yongmei Tian,Mingjun Cai,Haijiao Xu,Bohua Ding,Xian Hao,Junguang Jiang,Yingchun Sun,Hongda Wang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.8
The cell membrane provides critical cellular functions that rely on its elaborate structure and organization. The structure of turtle membranes is an important part of an ongoing study of erythrocyte membranes. Using a combination of atomic force microscopy and single-molecule force spectroscopy, we characterized the turtle erythrocyte membrane structure with molecular resolution in a quasi-native state. High-resolution images both leaflets of turtle erythrocyte membranes revealed a smooth outer membrane leaflet and a protein covered inner membrane leaflet. This asymmetry was verified by single-molecule force spectroscopy, which detects numerous exposed amino groups of membrane proteins in the inner membrane leaflet but much fewer in the outer leaflet. The asymmetric membrane structure of turtle erythrocytes is consistent with the semi-mosaic model of human, chicken and fish erythrocyte membrane structure, making the semi-mosaic model more widely applicable. From the perspective of biological evolution, this result may support the universality of the semi-mosaic model.
Zhong, Keli,Cai, Mingjun,Hou, Shuhua,Bian, Yanjiang,Tang, Lijun Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.2
A phenylbenzimidazole derivatized sensor (L) that behaves as a ratiometric fluorescent receptor for $Zn^{2+}$ in water has been described. In HEPES buffer at pH 7.4, sensor L displays a weak fluorescence emission band at 367 nm. Upon addition of $Zn^{2+}$, the emission intensity at 367 nm is decreased, concomitantly, a new emission band centered at 426 nm is developed, thus facilitates a ratiometric $Zn^{2+}$ sensing behavior. Sensor L binds $Zn^{2+}$ through a 1:1 binding stoichiometry with high selectivity over other metal cations. Sensor L displays a linear response to $Zn^{2+}$ concentration from 0 to $6.0{\times}10^{-5}M$, sensor L also exhibits high sensitivity to $Zn^{2+}$ with a detection limit of $3.31{\times}10^{-7}M$.
Li-Jun Tang,Pei Zhou,Zhenlong Huang,Jia Zhao,Mingjun Cai 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.10
Fluorescent and colorimetric recognition properties of 2-(4-N-phenyl-3-thiosemicarbazone)-8-hydroxyquinoline (1) in buffered aqueous solution (1% DMSO, HEPES 20 mM, pH = 7.4) have been examined. Sensor 1 displays highly selective and sensitive recognition to Cu2+ with fluorescence “ON-OFF” performance. The in situ formed 1-Cu2+ complex exhibits an excellent selectivity toward sulfide ions with fluorescence “OFF-ON” behavior via Cu2+ displacement approach. Thus, relay recognition of Cu2+ and sulfide by a known molecule 1 has been achieved.
Li-Jun Tang,Pei Zhou,Zhikai Qi,Zhenlong Huang,Jia Zhao,Mingjun Cai 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.8
A new quinoline-based acylhydrazone (1) has been synthesized and applied as a fluorescent probe. Probe 1 exhibits high selectivity and sensitivity to Cu2+ with fluorescence “ON-OFF” behavior in HEPES buffered (1‰ DMSO, HEPES 20 mM, pH = 7.4) solution. The on-site generated 1-Cu2+ complex displays excellent selectivity to sulfide ions with fluorescence “OFF-ON” performance through copper displacement approach.
Tang, Lijun,Zhou, Pei,Qi, Zhikai,Huang, Zhenlong,Zhao, Jia,Cai, Mingjun Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.8
A new quinoline-based acylhydrazone (1) has been synthesized and applied as a fluorescent probe. Probe 1 exhibits high selectivity and sensitivity to $Cu^{2+}$ with fluorescence "ON-OFF" behavior in HEPES buffered (1‰ DMSO, HEPES 20 mM, pH = 7.4) solution. The on-site generated 1-$Cu^{2+}$ complex displays excellent selectivity to sulfide ions with fluorescence "OFF-ON" performance through copper displacement approach.
Tang, Lijun,Zhou, Pei,Huang, Zhenlong,Zhao, Jia,Cai, Mingjun Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.10
Fluorescent and colorimetric recognition properties of 2-(4-N-phenyl-3-thiosemicarbazone)-8-hydroxyquinoline (1) in buffered aqueous solution (1% DMSO, HEPES 20 mM, pH = 7.4) have been examined. Sensor 1 displays highly selective and sensitive recognition to $Cu^{2+}$ with fluorescence "ON-OFF" performance. The in situ formed 1-$Cu^{2+}$ complex exhibits an excellent selectivity toward sulfide ions with fluorescence "OFF-ON" behavior via $Cu^{2+}$ displacement approach. Thus, relay recognition of $Cu^{2+}$ and sulfide by a known molecule 1 has been achieved.