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        Analysis of multi-coalbed CBM development methods in western Guizhou, China

        Zhaobiao Yang,Yong Qin,Tongsheng Yi,Jun Tang,Zhengguang Zhang,Congcong Wu 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.2

        After more than 20 years of development, the exploration and production of multi-bed coalbed methane in western Guizhou is now in a key growth phase. Based on geological characteristics of CBM and requirements of main development engineering in this area, we have looked into suitable fracturing and drainage modes for this area. This study shows that the Liupanshui coalfield has a high stress field, so staged fracturing with large discharge and high volume of fracturing fluid and sand and increase of the used quantity of high strength ceramic beads is effective for this kind of multi-bed coal reservoir. In contrast, Zhina coalfield has a relatively low stress field, and the Qianbei coalfield has transitional stress conditions. Separate layer fracturing by sleeve diverter in the Zhijin block ensures effective fracturing of multi-production layers. This method enhances fracturing efficiency and decreases fracturing cost and is therefore recommended for the whole area. Coiled tubing fracturing widely used in other countries is recommended for the Zhina and Qianbei coalfields. Commingled drainage and production is considered the best choice for the development of multi-bed CBM reservoirs. The basic geologic conditions for commingled drainage production of multiple layers are that the main productive layers can be ensured to produce gas preferentially and smoothly and the critical desorption pressure liquid level of other productive layers are above the roof depth of the main productive layers. On this basis, all the layers can be produced in an orderly manner by regulating the casing pressure and liquid level depth in different draining phases.

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        Ammonia etched petroleum pitch-based porous carbon as efficient catalysts for CO2 electroreduction

        Ning Hui,Guo Zhihao,Wang Wenhang,Wang Xiaoshan,Yang Zhongxue,Ma Zhengguang,Tian Yangming,Wu Chenghao,Hao Jian,Wu Mingbo 한국탄소학회 2022 Carbon Letters Vol.32 No.3

        Electrochemical reduction of carbon dioxide to valuable chemicals is a promising way of storing renewable energy through electric-to-chemical energy conversion, while its large-scale application is in urgent need of cheap and high-performance catalysts. Herein, we invent a convenient method to synthesize N-doped porous carbon by ammonia etching the pyrolysis carbon of petroleum pitch. We found the ammonia etching treatment not only increase the pyridinic-N content, but also enlarge the specific surface area of the petroleum pitch-based porous carbon. As a cheap and easily available catalyst for carbon dioxide electroreduction, up to 82% of Faradaic efficiency towards carbon monoxide was obtained at − 0.9 V vs the reversible hydrogen electrode in 0.1 M KHCO3. After a long time electrocatalysis of more than 20 h, the Faradaic efficiency of carbon monoxide remains 80%, indicating the porous carbon as made have an ultra-high stability as catalyst for carbon dioxide reduction. Our work provides a new technology to economically prepare efficient electrocatalysts for carbon dioxide reduction.

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        Effect of Pectin on the Expression of Proteins Associated with Mitochondrial Biogenesis and Cell Senescence in HT29-Human Colorectal Adenocarcinoma Cells

        Jose Javier Zamorano-Leon,Sandra Ballesteros,Natalia de las Heras,Luis Alvarez-Sala,Mariano de la Serna-Soto,Khaoula Zekri-Nechar,Gala Freixer,Bibiana Calvo-Rico,Zhengguang Yang,Jose Manuel Garcia-Gar 한국식품영양과학회 2019 Preventive Nutrition and Food Science Vol.24 No.2

        Mitochondria dynamic is regulated by different proteins, maintaining a balance between fission and fusion. An imbalance towards mitochondrial fission has been associated with tumor cell proliferation. The aim of this study was to analyze whether pectin modifies the viability of human colon cancer cells and the expression of proteins involved in mitochondrial fusion and fission. The human colon carcinoma cell line HT29 cells was growth in 10% fetal bovine serum in the absence and presence of pectin. Pectin reduced HT29 cell viability in a concentration-dependent manner, reaching a plateau at 150∼300 μ㏖/L pectin. The presence of 200 μ㏖/L pectin reduced the expression of dynamin-related protein-1 and increased expression of the mitochondrial fusion-associated proteins mitofusin-1 and 2. Expression of cyclin B1, a protein involved in G2/M transition, was found decreased in pectin-incubated HT29 cells. Moreover, expression of p53 protein, the amount of p53 in the nucleous and β-galactosidase activity, which are all biomarkers for cellular senescence, were significantly higher in pectin-incubated HT29 cells than in HT29 cells incubated without pectin. Expression of the protein B-cell lymphoma 2 (Bcl-2) homologous antagonist/killer was increased in response to incubation with pectin. However, incubation with pectin did not affect expression of Bcl-2-associated X protein or Bcl-2, or the caspase-3 activity. Overall, we concluded that pectin reduces the viability of human HT29 colon cancer cells, which is accompanied with a shift in the expression of proteins associated with mitochondrial dynamics towards mitochondrial fusion. Moreover, incubation with pectin favors cellular senescence over apoptosis in HT29 cells.

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