Reference gene selection for RT-qPCR analysis in two invasive whiteflies after the acquisition of vectored or non-vectored viruses

Zhen-Hong Lv,Hui-Peng Pan,Wei Zhang,Tian-Bo Ding,Dong Chu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

Custom reference gene selection is essential for reverse transcriptase-quantitative polymerase chain reaction(RT-qPCR) in different species of insects and various experiment conditions. In this study, 14 candidate referencegenes (HSP40, HSP20, HSP70, HSP90, v-ATPase, RPL29, EF-1, SDHA, Actin, PPIA, GAPDH, MyosinL,NADH, and γ-tubulin) were analyzed using five different programs including ΔCt method, BestKeeper, geNorm,NormFinder, and ReFinder to validate their use as reference genes in two invasive whiteflies, Bemisia tabaci Band Q, after acquiring the vectored virus, Tomato yellow leaf curl virus (TYLCV), or ingesting the non-vectoredvirus, Tomato spotted wilt virus (TSWV), respectively. The results showed that HSP40, v-ATPase, and EF-1 werethe most stable genes in B. tabaci B (B. tabaci B feeding on the healthy, TYLCV- and TSWV-infected tomatoplant), PPIA, SDHA, and RPL29 were the most stable genes in B. tabaci Q (B. tabaci Q feeding on the healthy,TYLCV- and TSWV-infected tomato plant). In addition, EF-1, RPL29, and HSP20 were the most stable referencegenes in B. tabaci B and Q. These findings provide the basis for future RT-qPCR-based studies on whitefly-virus interactions. Meanwhile,this report may set a precedent for reference gene selection in insects after the ingestion of non-vectored viruses.

Inhibition of microRNA-214 promotes epithelial mesenchymal transition process and induces interstitial cystitis in postmenopausal women by upregulating Mfn2

Jian-Wei Lv,Wei Wen,Chen Jiang,Qi-Bo Fu,Yin-Jun Gu,Ting-Ting Lv,Zhen-Dong Li,Wei Xue 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Our study aims to investigate the roles that microRNA-214 (miR-214) plays in the epithelial mesenchymal transition (EMT) process and the development of interstitial cystitis (IC) in postmenopausal women by targeting Mitofusin 2 (Mfn2). IC bladder tissues and adjacent normal bladder tissues were collected from postmenopausal women. Immunohistochemistry (IHC) staining was conducted. The target relationship between miR-214 and Mfn2 was determined by a dual luciferase reporter gene assay. Adipose-derived mesenchymal stem cells (ADMSCs) were extracted from postmenopausal rats and assigned to the blank, mimics, miR-214 inhibitors, mimics negative control (NC), inhibitors NC, Mfn2 siRNA, miR-214 inhibitors and Mfn2 siRNA groups. Exosomes secreted by transfected ADMSCs were instilled into the bladders of postmenopausal rats. The expression of miR-214 and Mfn2 mRNA and EMT markers was assessed by qRT-PCR and western blotting. It was confirmed that Mfn2 was the target gene of miR-214 in IC. Compared with the normal bladder tissues, miR-214 decreased, but Mfn2 increased in IC bladder tissues. Compared with the blank group, the expression of miR-214 and the expression levels of N-cadherin, Fibronectin, Twist1, Snail and Vimentin mRNA and protein increased, whereas the expression levels of Mfn2, E-cadherin and ZO-1 mRNA and protein decreased in the miR-214 mimics and Mfn2 groups. The expression of MiR-214 and the expression levels of N-cadherin, Fibronectin, Twist1, Snail and Vimentin mRNA and protein decreased, whereas the expression levels of Mfn2, E-cadherin and ZO-1 mRNA and protein increased in the miR-214 inhibitors group. Our findings indicate that the inhibition of miR-214 promotes the EMT process and contributes to bladder wall fibrosis by up-regulating Mfn2, thus leading to the occurrence of IC in postmenopausal women.

Study of failure analysis and manufacturing processes improvement of cracking connecting rod

Zhen Zhang,Anyuan Jiao,Xiwei Lv,Haodong Ding 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.11

Herein, we provide a novel method of using finite element analysis to guide the improvement of manufacturing processes on the basis of physicochemical analysis. First, based on the theoretical analysis of the force of the connecting rod, the solid model of cracking connecting rod is established by SolidWorks. Then the static stress analysis, modal analysis and fatigue analysis are carried out by ANSYS, determining the location where the connecting rod is prone to failure. Second, the influence of processing technology and other factors on the failure of connecting rod is analyzed, and several improved schemes for the manufacturing process are proposed. Finally, the fatigue test of the connecting rod is performed to verify the effect. Compared with the unimproved connecting rod, the tension and pressure of the improved connecting rod increased to 92.34 KN and -425.7 KN, respectively. The safety factor under this load is 1.8, which has increased by 20 % compared to 1.5 before the improvement.

Low-Temperature Selective Catalytic Reduction of NO with NH3 Over Mn-Fe Mixed Metal Oxides on the Nitric Vapor Functionalized Carbon Nanotubes Prepared by a Polyol Process

Xin Lv,Liheng Zhang,Sheng Zhan,Zhen Li,Yi Zeng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.4

Highly dispersed Mn-Fe mixed metal oxides were supported on the nitric vapor functionalized carbon nanotubes (CNTs) by the polyol process for the low-temperature selective catalytic reduction (SCR) of NO with NH3. X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), H2 temperature-programmed reduction (H2-TPR) and Fourier transform infrared (FT-IR) spectroscopy have been used to elucidate the structure and surface properties of the obtained catalysts. It was found that the Mn-Fe/ VF-CNTs catalysts synthesized by the polyol process exhibited higher activity and more extensive operating-temperature window, compared to the catalysts prepared by the impregnation method. On the basis of the catalyst characterization, the better dispersion of metal oxides on the CNTs surface, the more chemisorbed oxygen species, the higher Mn4+/Mn and Oα/Oα+Oβ ratios played key roles in the excellent catalytic performance of the catalyst in the low-temperature SCR of NO to N2 with NH3.

Chemical Characterization and Antitumor Activity of an Exopolysaccharide from Pholiota Squarrosa Quel. AS 5.245

Hai-zhen Zhao,Juan Wang,Fengxia Lv,Xiaomei Bie,Zhaoxin Lu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.2

A water-soluble exopolysaccharide (PESP-1) was extracted and purified using DEAE Sephadex A-50 and Sephadex G-100 columns from a submerged culture broth of Pholiota squarrosa Quel. AS 5.245. PEPS-1 was investigated for antitumor activity against Heps tumors implanted in mice. An inhibition rate of 78.46% at a dosage of 100 mg/kg was observed. PEPS-1 significantly (p<0.05) increased the relative spleen/thymus indices of Heps tumor-bearing mice at a dosage of 100 mg/kg, compared with controls. Antitumor properties were probably related to stimulation of the immune response. Preliminary physicochemical analysis identified PEPS-1 as a heteropolysaccharide mainly containing D-mannose, D-glucose and D-galactose at molar ratios of 50:33:18. Small amounts of D-rhamnose and D-xylose were also detected. The average Mw of PEPS-1 was 3.26×104 Da. Structural features probably played an important role in the antitumor activity of PEPS-1.

Fatigue Life Analysis and Experimental Study of the Input Shaft of 6-Speed Automatic Transmission

려건화(Jianhua Lv),종흥(Xing Zhong),주서(Rui Zhou),진진(Zhen Qin),장기(Qi Zhang),류성기(Sungki Lyu) Korean Society for Precision Engineering 2020 한국정밀공학회지 Vol.37 No.8

Online Recognition Method for Target Maneuver in UAV Autonomous Air Combat

Yicong Li,Zhen Yang,Xiaofeng Lv,Jichuan Huang,Yiyang Zhao,Deyun Zhou 제어로봇시스템학회 2022 제어로봇시스템학회 국제학술대회 논문집 Vol.2022 No.11

In unmanned aerial vehicle (UAV) autonomous air combat, target maneuver online recognition is beneficial to predict the tactical intention of the target, which is of great significance to air combat situational awareness and decision-making assistance. However, the target trajectory data acquired by our airborne sensors may contain one or more maneuvers. The existing research methods mainly focus on the recognition of single maneuver trajectory which has been segmented according to maneuver segments or the segmentation of target maneuver trajectory by introducing human experience. The above methods can not meet the requirements of online recognition of the unknown continuous multi-segment maneuvers trajectory in autonomous air combat. In this paper, the online recognition problem of target maneuver is transformed into three classification problems, which are maneuver switch subsequence recognition problem, maneuver switch point localization problem and single maneuver recognition problem. The cascaded classification networks based on Long Short-Term Memory (LSTM) temporal feature extraction is used to map maneuver trajectory to maneuver category sequence. The algorithm proposed in this paper is tested by randomly selecting target trajectories, which can automatically segment the trajectories and recognize the correct categories. The average sequence similarity between the predicted maneuver category sequence and the real maneuver category sequence after segmentation and recognition on the test set is above 0.9. The feasibility and effectiveness of the proposed algorithm are verified.

Development and Application of Polymorphic SSR Markers in Luffa cylindrica

Junjie Cui,Zhen Lv,Tianwen Yang,Jing Wang,Yu Hong 한국원예학회 2022 원예과학기술지 Vol.40 No.5

Two published genomes of Luffa cylindrica, ‘SG2019’ and ‘SO3’, were used to develop polymorphic SSR markers for L. cylindrica by searching and comparing the SSR motifs at the same site in the two genomes. Based on the SSR search conditions and primer design criteria, 2130 polymorphic SSR markers for L. cylindrica were developed. The main motif type was dinucleotide, accounting for 80.28% of the total; the main motif units were AT/AT and AAT/ATT, accounting for 87.80% of the total. Furthermore, 40 polymorphic SSR markers developed in this study were randomly selected and amplified in 24 Luffa samples. According to the results, the 40 polymorphic SSR markers showed an amplification rate of 100% and a polymorphism rate of 80%. Cluster analysis classified the 24 Luffa samples into two main groups, L. cylindrica and L. acutangula. Overall, the polymorphic SSR markers developed in this study display a high polymorphic rate and reliable utilization value.

Evaluation of Bending Fatigue Testing of Austempered Ductile Iron Spur Gears

Jian Hua Lv(려건화),Rui Zhou(주서),Yang Xu(허양),Zhen Qin(진진),Qi Zhang(장기),Sungki Lyu(류성기) 한국기계가공학회 2020 한국기계가공학회지 Vol.19 No.12

An experimental evaluation of bending fatigue strength for austempered ductile iron (ADI) spur gears was performed using a Zwick fatigue tester. The gear material was manufactured using vertical continuous casting, resulting in the radius of the graphite grains being smaller. The stress-number of cycles curve (S-N curve) for the bending fatigue strength of the ADI spur gears thus manufactured, without any specific surface treatments, was obtained using post-processing software. It was observed that when the reliability was 50%, the allowable root stress was 610 MPa. was calculated using an analytical method as well as the finite element method, and the difference between the values calculated using the two methods is only 7%. This study provides a reliable basis to rate the reliability design of small gearboxes in automation in the future.

MAPPING THE IN-FEED NATIVE ADVERTISING RESEARCH: A BIBLIOMETRIC REVIEW AND DIRECTIONS FOR FUTURE RESEARCH

Jun Yan,Chenying Hai,Zhen Li,Hewei Lv 글로벌지식마케팅경영학회 2023 Global Marketing Conference Vol.2023 No.07

Over the past decade, in-feed native advertising has become one of the most popular forms of online advertising, attracting attention from both academia and industry. Compared to traditional online ads, in-feed native ads are less intrusive and have more engaging content, effectively reducing users' tendency to avoid ads and improving advertising effectiveness. Despite existing review studies, most of them focus on specific aspects of in-feed native advertising, such as ad identification, disclosure, or regulation, and lack an overview of the relevant literature. Moreover, with the diversified development of digital media forms, the widespread application of AI technologies, and the improvement of consumers' ad literacy, in-feed native ad research faces new issues and practical challenges. Thus, a systematic review of existing research is necessary to identify valuable future research directions.