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- 저자 : Fengxia Lv (검색결과 3 건)
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Fengxia Lv,Chong Zhang,Fangfang Guo,Yingjian Lu,Xiaomei Bie,Hui Qian,Zhaoxin Lu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.1
The gene encoding the fibrinolytic enzyme (PPFE-I) from Paenibacillus polymyxa EJS-3 was cloned and sequenced (Genbank No. KC176802). The 1773 bp gene encoded 590 amino acids and had low homology with other known fibrinolytic enzymes. PPFE-I was soluble and expressed in E. coli BL21 to enhance the activity. The recombinant enzyme (rPPFE-I) was purified to homogeneity, and enzymatic properties were characterized. The optimal temperature and pH for rPPFE-I were 37℃ and 7.5, respectively. Observed activities of rPPFE-I were highest in the presence of Zn2+, Mg2+, and Fe2+ and the lowest in the presence of Ca2+ and Cu2+. The rPPFE-I activity was strongly inhibited by PMSF. The α-chain was affected by the rPPFE-I cleavage speed of fibrinogen, followed by the b and γ chains. The inhibitory effects of rPPFE-I against ADP-induced human platelet aggregation were dosedependent with an IC50 value of 1.54 μM.
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Hai-zhen Zhao,Juan Wang,Fengxia Lv,Xiaomei Bie,Zhaoxin Lu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.2
A water-soluble exopolysaccharide (PESP-1) was extracted and purified using DEAE Sephadex A-50 and Sephadex G-100 columns from a submerged culture broth of Pholiota squarrosa Quel. AS 5.245. PEPS-1 was investigated for antitumor activity against Heps tumors implanted in mice. An inhibition rate of 78.46% at a dosage of 100 mg/kg was observed. PEPS-1 significantly (p<0.05) increased the relative spleen/thymus indices of Heps tumor-bearing mice at a dosage of 100 mg/kg, compared with controls. Antitumor properties were probably related to stimulation of the immune response. Preliminary physicochemical analysis identified PEPS-1 as a heteropolysaccharide mainly containing D-mannose, D-glucose and D-galactose at molar ratios of 50:33:18. Small amounts of D-rhamnose and D-xylose were also detected. The average Mw of PEPS-1 was 3.26×104 Da. Structural features probably played an important role in the antitumor activity of PEPS-1.
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Effects of Fengycin from Bacillus subtilis fmbJ on Apoptosis and Necrosis in Rhizopus stolonifer
Qunyong Tang,Xiaomei Bie,Zhaoxin Lu,Fengxia Lv,Yang Tao,Xiaoxu Qu 한국미생물학회 2014 The journal of microbiology Vol.52 No.8
The lipopeptide antibiotic fengycin, produced by Bacillussubtilis, strongly inhibits growth of filamentous fungi. Inthis study, we evaluated the effects of fengycin treatment onapoptosis and necrosis in Rhizopus stolonifer by means ofcell staining and epifluorescence microscopy. At fengycinconcentrations less than 50 μg/ml, treated fungal cells demonstrateda dose-dependent increase in apoptosis-associatedmarkers compared with the untreated control. These markersincluded chromatin condensation, reactive oxygen speciesaccumulation, mitochondrial membrane potential depolarization,phosphatidylserine externalization, and the occurrenceof DNA strand breaks. These results showed that fungalcells were impaired in a number of important functionsand entered apoptosis upon treatment with low concentrationsof fengycin. In contrast, high concentrations (>50 μg/ml)induced necrosis, indicating that the fungicidal action offengycin operates via two modes: apoptosis at low concentrationsand necrosis at high concentrations. Additionally,the apoptotic effect that we have shown suggests that lowerconcentrations of fengycin than previously thought may beeffective for food preservation.
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