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      • KCI등재

        Preparation of biomass-based yam solar absorber for enhanced solar evaporation application

        Ting Li,Juan Jia,Yanqing Wang,Hanxue Sun,Jiyan Li,Zhaoqi Zhu,Weidong Liang 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.11

        The solar evaporation system has become a research hotspot in the field of photothermal conversion technology in recent years because of its high photothermal conversion efficiency and practicality of promotion. Biomass solar absorbers have excellent solar absorption properties and high solar-water vapor conversion, but they have the limitations of long feedstock production cycle and high carbonization temperature. As a kind of biomass materials, yam is not only inexpensive, but also has a short production cycle and rich pore structures. Therefore, in this paper, a yam based solar absorber with rich pore structure was prepared, and the photothermal conversion efficiency of the absorber was further investigated. The yam was treated with freeze-dried method and carbonized at different temperatures to obtain yam solar absorbers. Then the thermal stability and porosity of solar absorber was found to gradually rise as the treatment temperature increased. The solar absorption rate of the carbonized yam was close to 90% in the near UV-visible region. Solar-water vapor control experiments with different treatments of the solar absorber of the yam were carried out in simulated solar-driven interface steam generation system (SISGS). An absorber treated at 200 °C in the sun was found to have the best performance with a solar-water vapor conversion of about 90% and a water evaporation rate of 1.3164 kg m−2 h−1.

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        Single-cell transcriptomic analysis reveals transcriptional and cell subpopulation differences between human and pig immune cells

        Li Jie,Xu Yanan,Zhang Jiayu,Zhang Zhaoqi,Guo Han,Wei Dong,Wu Changhong,Hai Tang,Sun Hai-Xi,Zhao Yong 한국유전학회 2024 Genes & Genomics Vol.46 No.3

        Background The pig is a promising donor candidate for xenotransplantation. Understanding the differences between human and swine immune systems is critical for addressing xenotransplant rejection and hematopoietic reconstitution. The gene transcriptional profile differences between human and pig immune cell subpopulations have not been studied. To assess the similarities and differences between pigs and humans at the levels of gene transcriptional profiles or cell subpopulations are important for better understanding the cross-species similarity of humans and pigs, and it would help establish the fundamental principles necessary to genetically engineer donor pigs and improve xenotransplantation. Objective To assess the gene transcriptional similarities and differences between pigs and humans. Methods Two pigs and two healthy humans’ PBMCs were sorted for 10 × genomics single-cell sequence. We generated integrated human-pig scRNA-seq data from human and pig PBMCs and defined the overall gene expression landscape of pig peripheral blood immune cell subpopulations by updating the set of human-porcine homologous genes. The subsets of immune cells were detected by flow cytometry. Results There were significantly less T cells, NK cells and monocytes but more B cells in pig peripheral blood than those in human peripheral blood. High oxidative phosphorylation, HIF-1, glycolysis, and lysosome-related gene expressions in pig CD14+ monocytes were observed, whereas pig CD14+ monocytes exhibited lower levels of cytokine receptors and JAK-STAT-related genes. Pig activated CD4+T cells decreased cell adhesion and inflammation, while enriched for migration and activation processes. Porcine GNLY+CD8+T cells reduced cytotoxicity and increased proliferation compared with human GNLY+CD8+T cells. Pig CD2+CD8+γδT cells were functionally homologous to human CD2+CD4+ γδT cells. Pig CD2−CD8−γδT cells expressed genes with quiescent and precursor characteristics, while CD2−CD8+γδT cells expressed migration and memory-related molecules. Pig CD24+ and CD5+B cells are associated with inflammatory responses. Conclusion Our research with integrated scRNA-seq assays identified the different distribution of pig immune cell subpopulations and the different transcriptional profiles of human and pig immune cells. This study enables a deeper understanding of the development and function of porcine immune cells. Background The pig is a promising donor candidate for xenotransplantation. Understanding the differences between human and swine immune systems is critical for addressing xenotransplant rejection and hematopoietic reconstitution. The gene transcriptional profile differences between human and pig immune cell subpopulations have not been studied. To assess the similarities and differences between pigs and humans at the levels of gene transcriptional profiles or cell subpopulations are important for better understanding the cross-species similarity of humans and pigs, and it would help establish the fundamental principles necessary to genetically engineer donor pigs and improve xenotransplantation. Objective To assess the gene transcriptional similarities and differences between pigs and humans. Methods Two pigs and two healthy humans’ PBMCs were sorted for 10 × genomics single-cell sequence. We generated integrated human-pig scRNA-seq data from human and pig PBMCs and defined the overall gene expression landscape of pig peripheral blood immune cell subpopulations by updating the set of human-porcine homologous genes. The subsets of immune cells were detected by flow cytometry. Results There were significantly less T cells, NK cells and monocytes but more B cells in pig peripheral blood than those in human peripheral blood. High oxidative phosphorylation, HIF-1, glycolysis, and lysosome-related gene expressions in pig CD14+ monocytes were observed, whereas pig CD14+ monocytes exhibited lower levels of cytokine receptors and JAK-STAT-related genes. Pig activated CD4+T cells decreased cell adhesion and inflammation, while enriched for migration and activation processes. Porcine GNLY+CD8+T cells reduced cytotoxicity and increased proliferation compared with human GNLY+CD8+T cells. Pig CD2+CD8+γδT cells were functionally homologous to human CD2+CD4+ γδT cells. Pig CD2−CD8−γδT cells expressed genes with quiescent and precursor characteristics, while CD2−CD8+γδT cells expressed migration and memory-related molecules. Pig CD24+ and CD5+B cells are associated with inflammatory responses. Conclusion Our research with integrated scRNA-seq assays identified the different distribution of pig immune cell subpopulations and the different transcriptional profiles of human and pig immune cells. This study enables a deeper understanding of the development and function of porcine immune cells.

      • KCI등재

        The Perception of Technology Acceptance and Values Regarding ‘Sharing Economy Services’ as well as the ‘Network Effect’ on the ‘Use Intention’

        김승곤,설현도,Zhaoqi Li 한국무역연구원 2020 무역연구 Vol.16 No.5

        Purpose We studied the perceived value, risk and network effect on the intention of using the sharing service and verified how each variable affected the use of the shared service and the existence of variable differences. Design/Methodology/Approach To achieve the goal of this study, the structural model was presented based on precious research and the ‘perceived value’ (economic, empirical, reciprocal value), ‘perceived risk’, ‘perceived network effect’ were used as independent variables that affect the parameter, ‘trust’ and ‘perceived usefulness’, For the dependent variables, ‘use intention’ was put. The Structural Equation Model, as such, was constricted and criteria for suitability of the Structural Model were obtained by using this model, the relationship between independent and dependent variables was explored. Finding First, the perceived value had a significant effect on trust. Second, we confirmed that perceived risks of sharing economic services had a negative effect on trust and not on perceived usefulness. Third, the perceived usefulness and trust in sharing economic services had a significant impact on the intent of use. Fourth, this study showed that perceived risk of car-sharing services was the factor that gave direct negative effect on trust in service. Fifth, the effects of trust in vehicle-sharing services and perceived usability of the intended use were empirically verified. Research Implications Although research on vehicle sharing services and related companies is still lacking, the research on vehicle sharing services is expected to continue to grow and develop in the future, so follow-up study in various fields is needed.

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