The Up-Regulation of miR-199b-5p in Erythroid Differentiation Is Associated with GATA-1 and NF-E2

Yuxia Li,Hua Bai,Zhongzu Zhang,Weihua li,Lei Dong,Xueju Wei,Yanni Ma,Junwu Zhang,Jia Yu,Guotao Sun,Fang Wang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.3

MicroRNAs (miRNAs) represent a class of small non-co-ding regulatory RNAs that play important roles in normal hematopoiesis, including erythropoiesis. Although studies have identified several miRNAs that regulate erythroid commitment and differentiation, we do not understand the mechanism by which the crucial erythroid transcription factors, GATA-1and NF-E2 directly regulate and control differentiation via miRNA pathways. In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. Both GATA-1 and NF-E2 bound upstream of the miR-199b gene locus and activated its transcription. Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. Moreover, we identified c-Kit as a direct target of miR-199b-5p in erythroid cells. Taken together, our results establish a functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p and c-Kit, and provide new insights into the coupling of transcription and post-transcription regulation in erythroid differentiation.

Manganese dioxide-graphene nanocomposite film modified electrode as a sensitive voltammetric sensor of indomethacin detection

Yuxia Liu,Zhenfa Zhang,Cuizong Zhang,Wei Huang,Caiyun Liang,Jinyun Peng 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.8

Excess amount of analgesic and anti-inflammatory drug, such as indomethacin, often leads to serious gastrointestinal complications; therefore, amount of such active compound should be regulated in commercial drugs. This study proposes an efficient analytical technique to detect indomethacin selectively. We prepared and investigated electrochemical properties of a manganese dioxide-graphene nanocomposite film modified glassy carbon electrode (MnO2-Gr/GCE). The behavior of the modified electrode as electrocatalyst towards indomethacin oxidation was also examined. The cyclic voltammetric results reveal that the electrocatalytic activity for the oxidation of indomethacin can significantly be enhanced on the MnO2-Gr/GCE. Indomethacin exhibited a sensitive anodic peak at about 0.90 V at MnO2-Gr/GCE. The data obtained from differential pulse voltammetry showed that the anodic peak currents were linearly dependent on the indomethacin concentrations in the range of 1.0 × 10−7 to 2.5 × 10−5 mol/L with a detection limit of 3.2 × 10−8 mol/L (S/N = 3). Most importantly, the proposed method shows efficient and selective sensing of indomethacin in commercial pharmaceutical formulations. This is the first report of a voltammetric sensor for indomethacin using MnO2-Gr/GCE. We believe that this new method can be commercialized for routine applications in laboratories.

Overexpression of the receptor-like cytoplasmic kinase gene XCRK enhances Xoc and oxidative stress tolerance in rice

Yuxia Zhang,Xiaoling Guo,Yuchao Cui,Chiming Guo,Liang Chen 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5

In this paper, we characterized a differentiallyexpressed receptor-like cytoplasmic kinase XCRK, whichconfers resistance to bacterial leaf streak (BLS). We analyzedthe tissue expression of XCRK and showed that XCRK waswidely expressed in multiple rice (Oryza sativa) organs,including internodes, roots, leaves and flowers. In addition,the expression of XCRK was significantly induced by ABA,salt and H2O2 treatments, suggesting its function in thesepathways. The XCRK-overexpressing transgenic seedlingsexhibited higher tolerance to Xanthomonas oryzae pv.oryzicola(Xoc) compared with the wild-type seedlings. Furthermore,XCRK-overexpressing seedlings showed stronger antioxidantcapacity with reduced MDA and H2O2 content and higherantioxidant enzyme activities. It has been hypothesized thatthe enhanced Xoc tolerance was attributed to the improvedexpression of resistance-responsive factors positively regulatedby XCRK. In accordance with this, the expression of resistanceand oxidation-related genes Wrky77, Wrky13, PAL1, PR5,Fe-SOD and SodCc2 were up-regulated by the overexpressionof XCRK, which might contribute collectively to the increasedXoc tolerance. Overall, overexpression of XCRK couldenhance the antioxidant capacity and Xoc tolerance in rice.

Functional Roles of Ca _v 1.3(α _1D ) Calcium Channels in Atria : Insights Gained From Gene-Targeted Null Mutant Mice

Zhang, Zhao,He, Yuxia,Tuteja, Dipika,Xu, Danyan,Timofeyev, Valeriy,Zhang, Qian,Glatter, Kathryn A.,Xu, Yanfang,Shin, Hee-Sup,Low, Reginald,Chiamvimonvat, Nipavan Ovid Technologies Wolters Kluwer -American Heart A 2005 CIRCULATION - Vol.112 No.13

<P>Previous data suggest that L-type Ca2+ channels containing the Cav1.3(alpha(1D)) subunit are expressed mainly in neurons and neuroendocrine cells, whereas those containing the Cav1.2(alpha1C) subunit are found in the brain, vascular smooth muscle, and cardiac tissue. However, our previous report as well as others have shown that Cav1.3 Ca2+ channel-deficient mice (Cav1.3(-/-)) demonstrate sinus bradycardia with a prolonged PR interval. In the present study, we extended our study to examine the role of the Cav1.3(alpha1D) Ca2+ channel in the atria of Cav1.3(-/-) mice.</P>

Multiple-Period Repetitive Controller for Selective Harmonic Compensation with Three-Phase Shunt Active Power Filter

Zhang, Chao,Gong, Maofa,Zhang, Yijun,Li, Yuxia The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.3

This paper presents a shunt active power filter (SAPF) for compensating inter-harmonics and harmonics when inter-harmonics content is evident in the grid. The principle of inter-harmonics generation in the grid was analyzed, and the inter-harmonics effect on repetitive controllers was discussed in terms of control performance. Traditional repetitive controllers are not applicable in inter-harmonic compensation. Moreover, the effect of an ideal controller on harmonics signals was analyzed on the basis of the internal model principle. The repetitive controller was improved in the form of a basis function according to theoretical analysis. The finite-dimensional repetitive controller, which is also called the multiple-period repetitive controller, was designed for the control of multiple periodic signals. A selective harmonic compensation system was developed with SAPF. This system can be used to compensate harmonics and inter-harmonics in the grid. Finally, system control performance was verified by simulation and experimental results.

Insect cytokine growth-blocking peptide may regulate density-dependent phase trait of cuticular melanization in the larval armyworm, Mythimna separata

Zhang Yingchao,He Juan,Zhang Yuxia,Li Yue,Yu Yang,Jiang Xingfu,Pan Weidong 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2

Growth-blocking peptide (GBP) is a 25 amino acid insect cytokine found in lepidopteran insects that has diverse biological activities such as larval growth regulation, paralysis induction, cell proliferation and stimulation of immune cells. Density-dependent phase polyphenism is a phenomenon of phenotypic plasticity in which the expression of a variety of traits can be affected by local population density. In the present study, the armyworm Mythimna separata larvae with four rearing densities (1 larva/vial, 2 larvae/vial, 4 larvae/vial and 6 larvae/vial) were tested for cuticular melanization and body weight throughout the third-fifth instar, and the functional role of GBP in regulating the changes was investigated. The results indicated that when reared at high densities, the larvae exhibited less body weight and more degree of cuticular melanization than larvae reared at low densities. The gene expression of GBP in armyworm larvae showed an initial rise and then decline trend with increased rearing densities in the third to fifth instar. Compared with control, more degree of cuticular melanization was observed in GBP-injected larvae (500 ng/larva in volume 50 μL) than that in Ringer’s solution-injected counterparts. Furthermore, the gene expression level of dopa decarboxylase and prophenoloxidase increased significantly in GBP-injected fifth instar larvae from 6 h to 12 h after injection, suggesting the role of GBP in modulating density-dependent phase trait of armyworm cuticular melanization.

Multiple-Period Repetitive Controller for Selective Harmonic Compensation with Three-Phase Shunt Active Power Filter

Chao Zhang,Maofa Gong,Yijun Zhang,Yuxia Li 전력전자학회 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.3

This paper presents a shunt active power filter (SAPF) for compensating inter-harmonics and harmonics when inter-harmonics content is evident in the grid. The principle of inter-harmonics generation in the grid was analyzed, and the inter-harmonics effect on repetitive controllers was discussed in terms of control performance. Traditional repetitive controllers are not applicable in inter-harmonic compensation. Moreover, the effect of an ideal controller on harmonics signals was analyzed on the basis of the internal model principle. The repetitive controller was improved in the form of a basis function according to theoretical analysis. The finite-dimensional repetitive controller, which is also called the multiple-period repetitive controller, was designed for the control of multiple periodic signals. A selective harmonic compensation system was developed with SAPF. This system can be used to compensate harmonics and inter-harmonics in the grid. Finally, system control performance was verified by simulation and experimental results.

Cloning and Characterization of an Annexin Gene from Cynanchum komarovii that Enhances Tolerance to Drought and Fusarium oxysporum in Transgenic Cotton

Yongan Zhang,Qinghua Wang,Xue Zhang,Xingliang Liu,Ping Wang,Yuxia Hou 한국식물학회 2011 Journal of Plant Biology Vol.54 No.5

A novel plant annexin, CkANN, was isolated from Cynanchum komarovii. Sequence analysis showed that CkANN contained four conserved exdonexin fold regions, a putative peroxidase heme binding motif and two S3 clusters. Real-time PCR analysis indicated that the CkANN mRNAwas abundant in leaf, flower, root and stem and that its level is highest in leaf and flower. The transcription level of CkANN was increased significantly following stress by salicylic acid (SA), methyl jasmonate (MeJA), abscisic acid (ABA), NaCl, PEG 6000 (polyethylene glycol) and H_2O_2. Over-expression of CkANN in transgenic cotton caused a significant increase in tolerance to drought and led to higher levels of proline and soluble carbohydrates compared to wild-type (WT) plants. In response to drought,the transgenic plants also displayed higher total chlorophyll levels and reduced accumulation of thiobarbituric acidreactive substances (TBARS) compared to WT plants. Furthermore, the transgenic cotton showed enhanced resistance to Fusarium oxysporum via increased total peroxidase activity and induction of the expression of some pathogenesis-related proteins. In conclusion, this study has demonstrated that C. komarovii CkANN is involved in drought tolerance and disease resistance and therefore may contribute significantly to the development of drought- or disease-resistant crops.

The Up-Regulation of miR-199b-5p in Erythroid Differentiation Is Associated with GATA-1 and NF-E2

Li, Yuxia,Bai, Hua,Zhang, Zhongzu,li, Weihua,Dong, Lei,Wei, Xueju,Ma, Yanni,Zhang, Junwu,Yu, Jia,Sun, Guotao,Wang, Fang Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.3

MicroRNAs (miRNAs) represent a class of small non-coding regulatory RNAs that play important roles in normal hematopoiesis, including erythropoiesis. Although studies have identified several miRNAs that regulate erythroid commitment and differentiation, we do not understand the mechanism by which the crucial erythroid transcription factors, GATA-1and NF-E2 directly regulate and control differentiation via miRNA pathways. In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. Both GATA-1 and NF-E2 bound upstream of the miR-199b gene locus and activated its transcription. Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. Moreover, we identified c-Kit as a direct target of miR-199b-5p in erythroid cells. Taken together, our results establish a functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p and c-Kit, and provide new insights into the coupling of transcription and post-transcription regulation in erythroid differentiation.

The short-term impact of fine particulate matter pollution on the incidence of cardiovascular diseases in Beijing, China

Yuxia MA,Haoran JIAO,Yifan ZHANG,Bowen CHENG,Fengliu FENG 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-