Genome‑wide identification of GMP genes in Rosaceae and functional characterization of FaGMP4 in strawberry (Fragaria × ananassa)

Yuanxiu Lin,Jiahao Zhang,Lintai Wu,Yunting Zhang,Qing Chen,Mengyao Li,Yong Zhang,Ya Luo,Yan Wang,Xiaorong Wang,Haoru Tang 한국유전학회 2021 Genes & Genomics Vol.43 No.6

Background GDP-D-mannose pyrophosphorylase (GMP) is one of the key enzymes determining ascorbic acid (AsA) biosynthesis. However, little information about GMP genes is currently available for the Rosaceae species, especially in the AsA-riched cultivated octoploid strawberry (Fragaria × ananassa). Objective To identify the all the GMP genes in Rosaceae, as well as the predominant homologues and the role of GMP genes in strawberry AsA accumulation. Methods In the present study, we performed genome-wide identifcation and comprehensive analysis of the duplicated GMP genes in strawberry and other Rosaceae species by bioinformatics methods, the expression of the GMP genes from cultivated strawberry (Fragaria × ananassa, FaGMP) was specifcally analyzed by qPCR. Finally, the FaGMP4 was transiently overexpressed in strawberry to estimate the role of GMP in regulating AsA accumulation in strawberry. Results As results, a total of 28 GMP genes were identifed in the fve Rosaceae species. The origins of duplication events analysis suggested that most GMP duplications in Rosaceae species were generated from whole genome duplication (WGD). The Ka/Ks ratio suggested that FaGMP genes underwent a stabilization selection. qPCR based expression analysis showed diferent patterns of FaGMP paralogs during fruit ripening, while FaGMP4 expressed higher in the variety containing higher AsA. Overexpression of FaGMP4 in strawberry signifcantly enhanced AsA accumulation. Furthermore, the expression of FaGMP4 under the treatment of blue and red light was largely increased in leaves while signifcantly inhibited in fruit. These results revealed the vital role of FaGMP4 in regulating AsA in strawberry.

Study on Multi-Source Localization of Wireless Sensor Network Based on Quantitative Information

Yunting Liu,Hui Guo,Yuanwei Jing,Siying Zhang 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.4

Against the problem that quantitative localization is concentrated on single-source localization, this paper studies the quantitative multi-source localization methods based on wireless sensor network. Firstly, the log-quantization strategy which reflects the characteristics of acoustic source is proposed. The sensor node transmits the quantized information to base station according to the measurements and log-quantization strategy. Secondly the based station estimates the location of the sources according to the proposed method termed as multiple source localization based on possibilistic C means clustering algorithm. Finally the efficient of the proposed method is verified by the simulation under different parameters. The simulation results show that the proposed method could accurately estimate the location of source. And the proposed method is robust to the packet loss rate.

Preparation and Performance Study of Self-Cleaning TiO2/Kaolin-Finished Cotton Fabric

Qiang Zhao,Yunting Zhang,Zhenjiang Liu,Huiyan Ma,Yan Li,Xiaoping Gao 한국섬유공학회 2023 Fibers and polymers Vol.24 No.12

TiO2/kaolin was first synthesized by using ultrasound-sol–gel method and then deposited onto the surface of cotton fabrics through two dipping and two rolling process. To investigate the properties of this TiO2/kaolin-finished cotton fabric, TiO2/kaolin/cotton composites were characterized by Fourier transform infrared spectrum and energy dispersive spectrometer, while the surface morphologies of the cotton fabrics were analyzed by field emission scanning electron microscopy. The whiteness, air permeability, breaking strength and elongation of TiO2/kaolin/cotton composites were, respectively, measured and the results showed that all these parameters decreased slightly with the appearance of TiO2/kaolin. The UPF value of TiO2/kaolin/cotton composites was significantly enhanced to 100+ , even if after soaping for 30 times, which might be due to the synergistic effect of kaolin and TiO2. In addition, the performance such as hand feel attributes, softness, smoothness and stiffness were measured by PhabrOmeter, respectively. The stiffness of all finished fabrics was obviously better than that of the unfinished cotton. However, the softness and smoothness of finished fabrics were slightly decreased compared to unfinished cotton. Rhodamine B was used as a test contaminant to qualitatively assess the self-cleaning properties of the TiO2/kaolin-finished cotton fabric. The removal of Rhodamine B indicated that the TiO2/kaolin-finished fabric exhibited excellent photocatalytic properties. This study illustrates the great potential of the low-cost kaolin powder as an efficient finishing agent for the fabric finishing process, and meanwhile provides a novel suggestion for fabric modification.

Differential Diagnosis of Axillary Inflammatory and Metastatic Lymph Nodes in Rabbit Models by Using Diffusion-Weighted Imaging: Compared with Conventional Magnetic Resonance Imaging

Junping Wang,Qian Liao,Yunting Zhang,Chunshui Yu,Renju Bai,Haoran Sun 대한영상의학회 2012 Korean Journal of Radiology Vol.13 No.4

Objective: This experiment aims to determine the diagnostic value of diffusion-weighted imaging (DWI) in the differentiation of axillary inflammatory lymph nodes from metastatic lymph nodes in rabbit models in comparison with conventional magnetic resonance imaging (MRI). Materials and Methods: Conventional MRI and DWI were performed at 4 weeks after successful inoculation into the forty female New Zealand white rabbits’ mammary glands. The size-based and signal-intensity-based criteria and the relative apparent diffusion coefficient (rADC) value were compared between the axillary inflammatory lymph nodes and metastatic lymph nodes, with histopathological findings as the reference standard. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic performance of the aforementioned criteria and rADC value in differentiating the axillary inflammatory lymph nodes from metastatic lymph nodes. Results: Thirty-two axillary inflammatory lymph nodes and 46 metastatic ones were successfully isolated and taken into pathological analysis. The differences of the aforementioned criteria between the two groups were not statistically significant (p > 0.05). However, the rADC value of the inflammatory lymph nodes (0.9 ± 0.14) was higher than that of metastatic ones (0.7 ± 0.18), with significant difference (p = 0.016). When the rADC value was chosen as 0.80, the area under the ROC curve is greater than all other criteria, and the sensitivity, specificity, accuracy, positive predictive value, and negative predictive value for differentiating two groups were 86.2%, 79.3%, 81.2%, 84.2%, and 85.6%, respectively. Conclusion: Diffusion-weighted imaging is a promising new technique for differentiating axillary inflammatory lymph nodes from metastatic lymph nodes. Compared with routine magnetic resonance sequences, DWI could provide more useful physiological and functional information for diagnosis. Objective: This experiment aims to determine the diagnostic value of diffusion-weighted imaging (DWI) in the differentiation of axillary inflammatory lymph nodes from metastatic lymph nodes in rabbit models in comparison with conventional magnetic resonance imaging (MRI). Materials and Methods: Conventional MRI and DWI were performed at 4 weeks after successful inoculation into the forty female New Zealand white rabbits’ mammary glands. The size-based and signal-intensity-based criteria and the relative apparent diffusion coefficient (rADC) value were compared between the axillary inflammatory lymph nodes and metastatic lymph nodes, with histopathological findings as the reference standard. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic performance of the aforementioned criteria and rADC value in differentiating the axillary inflammatory lymph nodes from metastatic lymph nodes. Results: Thirty-two axillary inflammatory lymph nodes and 46 metastatic ones were successfully isolated and taken into pathological analysis. The differences of the aforementioned criteria between the two groups were not statistically significant (p > 0.05). However, the rADC value of the inflammatory lymph nodes (0.9 ± 0.14) was higher than that of metastatic ones (0.7 ± 0.18), with significant difference (p = 0.016). When the rADC value was chosen as 0.80, the area under the ROC curve is greater than all other criteria, and the sensitivity, specificity, accuracy, positive predictive value, and negative predictive value for differentiating two groups were 86.2%, 79.3%, 81.2%, 84.2%, and 85.6%, respectively. Conclusion: Diffusion-weighted imaging is a promising new technique for differentiating axillary inflammatory lymph nodes from metastatic lymph nodes. Compared with routine magnetic resonance sequences, DWI could provide more useful physiological and functional information for diagnosis.

A study of factors influencing disruptive innovation in Chinese SMEs

Jin Cheng,Zhaohui Zhu,Yunting Zhang 기술경영경제학회 2017 ASIAN JOURNAL OF TECHNOLOGY INNOVATION Vol.25 No.1

Although around 99% of companies in China today are small- and medium-sized enterprises(SMEs), their innovative status is not optimistic, being constrained by lack of funding, size,and experience. Nevertheless, disruptive innovation provides an opportunity for SMEs tosurpass the large companies. This paper analyses external and internal factors influencingdisruptive innovation in SMEs, and compares those factors between low-end disruption andhigh-end disruption. The results of this empirical study suggest that factors influencing highenddisruptive innovation include government support, external knowledge sources, strategicsupport, and dominant position of R&D; while the factors influencing low-end disruptiveinnovation are cooperation with venture capitalists, external knowledge sources, dominantposition of R&D, and entrepreneurs’ innovation willingness. Two case studies are describedto illustrate our results.

Effect of pre-slaughter fasting time on carcass yield, blood parameters and meat quality in broilers

Wu Xuezhuang,Zhou Yahao,Lu Zhentao,Zhang Yunting,Zhang Tietao 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.2

Objective: The aim of this study was to evaluate the effect of pre-slaughter fasting time on carcass yield, meat quality, blood parameters and glucose metabolism in broilers. Methods: Four hundred and fifty Arbor Acres (AA) broilers at 42 days of age were divided into 5 groups with 6 replicates in each group and 15 chickens as one replicate. Following this period, broilers from each group were distributed among five groups according to preslaughter fasting period as 4, 8, 12, 16, or 20 h. Results: With increasing fasting time, the carcass yield (p<0.01), the breast muscles yield (p<0.01) and the thigh yield (p<0.01) of the broilers were all linearly increased. With increasing fasting time, the L* values (p<0.01), cooking loss (p = 0.020), moisture content (p<0.01) in the leg muscles linearly downregulated, while the drip loss (p = 0.043), pH45 min (p<0.01) and pH24 h (p<0.01) were linearly upregulated. A trend for a lower (p = 0.071) shear force in the leg muscles was also observed in broilers fasted for longer time. Similar results were also found in breast muscles. The different fasting treatments did not influence the breast muscles glycogen content (p>0.10), while the increase of fasting time resulted in a linear decrease of the blood glucose (p = 0.021) and, more specifically, the glycogen content of the liver and leg muscles (p<0.001). With increasing fasting time, the aspartate transaminase (p<0.01), uric acid (p<0.01), and triglycerides (p<0.01) in serum linearly downregulated, while the alanine aminotransferase was linearly upregulated. Conclusion: The results of this study show a significant influence of fasting time on carcass yield and meat quality in broilers. Moderate fasting (8 to 12 h) before slaughter can reduce the weight loss of broilers. Prolonged fasting (≥16 h) increased body weight loss, decreased slaughtering performance and fluctuating blood indexes of broilers.

Evaluation of suitable qRT-PCR normalization genes for various citrus rootstocks

He Wen,Xie Rui,Li Huan,Wang Yan,Chen Qing,Lin Yuanxiu,Zhang Yunting,Luo Ya,Zhang Yong,Tang Haoru,Wang Xiaorong 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.1

Citrus rootstock can modify plant growth and enhance stress resistance. There are many genotypes and species used as citrus rootstocks. Although multiple citrus rootstocks whole genome sequence and transcriptome databases have previously been published, no suitable internal reference genes have been investigated for standardization of gene expression via quantitative real-time PCR. Here we reported the first systematic and comprehensive analysis of reference genes for quantitative real- time PCR standardization in various citrus rootstocks. The expression stability of ten candidate reference genes in diverse sample subsets of flooding, drought, alkaline and cold treatments was evaluated using four statistical algorithms, geNorm, NormFinder, BestKeeper and ΔCt method. The results demonstrated that the expression stability of reference genes varied under different experimental conditions. In addition, normalization of gene expression of 9-cis-epoxycarotenoid dioxygenase 3 (NCED 3), involved in abscisic acid biosynthesis, was conducted to further confirm the reliability of the reference genes. Overall, EF-1α, DIM 1, GAPC and UBC expressed much more stably. ACTIN and GAPDH were not recommended for normalization in given experimental conditions due to low stability. Our main contribution was to identify reference genes with suitable and stable expression in citrus rootstocks varied across selected experimental conditions. Furthermore, these results will provide valuable information for future research on citrus rootstocks.