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Zhang, Zhi-Guo,Li, Gang,Feng, Da-Yun,Zhang, Jian,Zhang, Jing,Qin, Huai-Zhou,Ma, Lian-Ting,Gao, Guo-Dong,Wu, Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1
Several recent studies have showed that the n-myc downstream regulated gene 2 (NDRG2) is a new tumor suppressor gene, and that it plays an important role in tumor suppression in several cancers or cancer cell lines. However, few studies focused on its function in neuroblastoma cells. In the present investigation, we demonstrated that NDRG2 overexpression inhibited their proliferation. Using a cDNA microarray, we found that overexpression of NDRG2 inhibited the expression of cysteine-rich protein 61 (CYR61), a proliferation related gene. From our research, CYR61 may partially hinder NDRG2-mediated inhibition of cell proliferation. Overexpression of NDRG2 resulted in accumulation of cells in the G1 phase, which was accompanied by upregulation of p21 and p27 and downregulation of CDK4 and cyclin D1. Taken together, these data indicate that NDRG2 inhibits the proliferation of neuroblastoma cells partially through suppression of CYR61. Our findings offer novel insights into the physiological roles of NDRG2 in neuroblastoma cell proliferation, and NDRG2 may prove to be effective candidate for the treatment of children with neuroblastoma.
A brain somatic RHEB doublet mutation causes focal cortical dysplasia type II
Shanshan Zhao,Zhenghui Li,Muxian Zhang,Lingliang Zhang,Honghua Zheng,Jinhuan Ning,Yanyan Wang,Feng-Peng Wang,Xiaobin Zhang,Hexia Gan,Yuanqing Wang,Xian Zhang,Hong Luo,Guojun Bu,Huaxi Xu,Yi Yao,Yun-wu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Focal cortical dysplasia type II (FCDII) is a cerebral cortex malformation characterized by local cortical structure disorganization, neuronal dysmorphology, and refractory epilepsy. Brain somatic mutations in several genes involved in the PI3K/AKT/mTOR pathway are associated with FCDII, but they are only found in a proportion of patients with FCDII. The genetic causes underlying the development FCDII in other patients remain unclear. Here, we carried out whole exome sequencing and targeted sequencing in paired brain–blood DNA from patients with FCDII and identified a brain somatic doublet mutation c.(A104T, C105A) in the Ras homolog, mTORC1 binding (RHEB) gene, which led to the RHEB p.Y35L mutation in one patient with FCDII. This RHEB mutation carrier had a dramatic increase of ribosomal protein S6 phosphorylation, indicating mTOR activation in the region of the brain lesion. The RHEB p.Y35L mutant protein had increased GTPλS-binding activity compared with wild-type RHEB. Overexpression of the RHEB p. Y35L variant in cultured cells also resulted in elevated S6 phosphorylation compared to wild-type RHEB. Importantly, in utero electroporation of the RHEB p.Y35L variant in mice induced S6 phosphorylation, cytomegalic neurons, dysregulated neuron migration, abnormal electroencephalogram, and seizures, all of which are found in patients with FCDII. Rapamycin treatment rescued abnormal electroencephalograms and alleviated seizures in these mice. These results demonstrate that brain somatic mutations in RHEB are also responsible for the pathogenesis of FCDII, indicating that aberrant activation of mTOR signaling is a primary driver and potential drug target for FCDII.
Inhibitory Effects of Phenolic Alkaloids of Menispermum Dauricum on Gastric Cancer in Vivo
Zhang, Hong-Feng,Wu, Di,Du, Jian-Kuo,Zhang, Yan,Su, Yun-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24
The present study was conducted to investigate effects and mechanisms of action of phenolic alkaloids of Menispermum dauricum (PAMD) on gastric cancer in vivo. In vitro, cell apoptosis of human gastric cancer cell line SGC-7901 was observed using fluorescence staining. In vivo, a mice model was constructed to observe tumor growth with different doses. Cell apoptosis was examined using flow cytometry and K-RAS protein expression using Western blotting. The mRNA expression of P53, BCL-2, BAX, CASPASE-3, K-RAS was examined by real-time PCR. PAMD significantly suppressed tumor growth in the xenograft model of gastric cancer in a dose-dependent manner (p<0.01). Functionally, PAMD promoted cell apoptosis of the SGC-7901 cells and significantly increased the rate of cell apoptosis of gastric tumor cells (p<0.05). Mechanically, PAMD inhibited the expression of oncogenic K-RAS both at the mRNA and protein levels. In addition, PAMD affected the mRNA expression of the cell apoptosis-related genes (P53, BCL-2, BAX, CASPASE-3). PAMD could suppress gastric tumor growth in vivo, possibly through inhibiting oncogenic K-RAS, and induce cell apoptosis possibly by targeting the cell apoptosis-related genes of P53, BCL-2, BAX, CASPASE-3.
Clinical Significance and Prognostic Value of Pentraxin-3 as Serologic Biomarker for Lung Cancer
Zhang, Dai,Ren, Wei-Hong,Gao, Yun,Wang, Nian-Yue,Wu, Wen-Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7
Purposes: Lung cancer is prevalent worldwide and improvements in timely and effective diagnosis are need. Pentraxin-3 as a novel serum marker for lung cancer (LC) has not been validated in large cohort studies. The aim of the study was to assess its clinical value in diagnosis and prognosis. Methods: We analyzed serum PTX-3 levels in a total of 1,605 patients with LC, benign lung diseases and healthy controls, as well as 493 non-lung cancer patients including 12 different types of cancers. Preoperative and postoperative data were further assessed in patients undergoing LC resection. The diagnostic performance of PTX-3 for LC and early-stage LC was assessed using receiver operating characteristics (ROC) by comparing with serum carcinoembryonic antigen (CEA), cytokeratin 19 fragments (CYFRA 21-1). Results: Levels of PTX-3 in serum were significantly higher in patients with LC than all controls. ROC curves showed the optimum diagnostic cutoff was 8.03ng/mL (AUC 0.823, [95%CI 0.789-0.856], sensitivity 72.8%, and specificity 77.3% in the test cohort; 0.802, [95%CI 0.762-0.843], sensitivity 69.7%, and specificity 76.4% in the validate cohort). Similar diagnostic performance of PTX-3 was observed for early-stage LC. PTX-3 decreased following surgical resection of LC and increased with tumor recurrence. Significantly elevated PTX-3 levels were also seen in patients with non-lung cancers. Conclusions: The present data revealed that PTX-3 was significantly increased in both tissue and serum samples in LC patients. PTX-3 is a valuable biomarker for LC and improved identification of patients with LC and early-stage LC from those with non-malignant lung diseases.
Genetic Analysis of Generalized S-Transform
Yun Lin,Xiaowan Yu,Chunguang Ma,Zheng Dou,Zhiqiang Wu,Zhiping Zhang 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.4
This text starts with the short time Fourier transform and continuous wavelet transform to deduce the generalized S-transformation. From the point of generation views, we analyzed a relative relationship between generalized S-transformation and the short time Fourier transform, and the other relative relationship between generalized S transform and continuous wavelet transform. The article gives the definition of “the gene mutation of formula” and “the genetic restructuring of formula”, and introduces the deriving process of the two core concept. Theoretical analyses show that generalized S-transformation inherited the desirable characteristics in short time Fourier transform which use the window function to select suitable signal. Through genome sequencing of specific parameters, generalized S-transformation has a stronger adaptation that the time-frequency window could make real-time adjusting of frequency. Moreover, generalized S-transformation breaks out limitation that the wavelet function has to content the admissible conditions. From the point of gene mutation, we give the definition of “the gene mutation of formula”. Based on the structure form of wavelet functions, we define the generalized S-transformation with a wider domain of definition. Generalized S-transformation inherited the desirable characteristics of the short time Fourier transform and continuous wavelet transform. It has great utility and flexibility in analyzing non-stationary signals.
Yun-fei Zhang,Man-geng Lu,Hui Liu,Yanguang Wu 한국고분자학회 2017 폴리머 Vol.41 No.2
In this study, effects of mixed hardeners (ammonium chloride mixed with ammonium persulfate, oxalate and phosphoric acid) on the properties of 2,4,6-trimethylolphenate modified UF resin (TMPUF) were studied, and the potlife, gel time and curing temperature of TMPUF/hardeners were characterized. The results indicated that the hardeners used have critical influence on the pH value of TMPUF and some proton donor can be used to lower the pH value of TMPUF with low formaldehyde content. Besides, bamboo particleboards were made with TMPUF/hardeners, and tensile properties, water absorption ratio and the swelling ratio of bamboo particleboards were characterized. The study demonstrated that the tensile strength of bamboo particleboards increased when partial ammonium chloride was replaced by ammonium persulfate to cure the TMPUF adhesives in our experiment.
Amelioration of radiation-induced liver damage by p-coumaric acid in mice
Yun-Hong Li,Jiang-Xue Wu,Qian He,Jia Gu,Lin Zhang,Hao-Zhi Niu,Xin-Wen Zhang,Han-Ting Zhao,Jia-Ying Xu,Li-qiang Qin 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.10
Radiation-induced liver damage (RILD) is a spiny problem in radiotherapy or other circumstances that exposure to radiation. The need for radioprotective agent is increasing to protect liver tissue. This study aimed to explore the hepatoprotective effect of p-coumaric acid (CA) against RILD. C57BL/6 male mice were exposed to 4 Gy irradiation and administrated with CA for 4 days starting on the same day of irradiation. Mice were sacrificed to obtain blood and liver tissues on day 3.5 or 14 post irradiation, respectively. The blood and liver tissues were collected. As compared with the only irradiated group, CA supplementation improved liver morphology, decreased serum alanine aminotransferase and aspartate aminotransferase, inhibited BCL2-associated X (BAX) protein expression, and improved the mice hematopoietic function. CA at the dose of 100 mg/kg body weight showed better effect compared to the other doses. Thus, CA might possess potential to protect against RILD.
The Recognition Method of Radiation Source Based on Information Entropy and Cloud Model
Yun Lin,Can Wang,Chunguang Ma,Zheng Dou,Zhiqiang Wu,Zhiping Zhang 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.2
Information entropy features are often used for radiation source signal recognition, but due to the information entropy is very sensitive to noise, so this method has greater recognition rate changes with the SNR. This paper putting forward a viable recognition based on Entropy and cloud model. using cloud model to extract secondary features of signals, build radiation source signal’s entropy and cloud feature vector. The method uses cloud model description and processing interval fuzzy and observation noise data, better solve the low SNR cases of radiation source signal feature extraction problem. At the same time, putting forward the similar cloud classification recognition algorithm based on cloud model. The simulation results show that Entropy and cloud model has better recognition effect under low SNR, which can improve the signals’ recognition rate under low SNR.