http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Dewang Kong,Min Zhao,Shikuo Li,Fangzhi Huang,Jiming Song,Yupeng Yuan,YUHUA SHEN,ANJIAN XIE 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.1
In this paper, a facile and efficient method combined sol–gel method with hydrothermal process was employed for preparing titanium dioxide (TiO2/reduced graphene oxide (rGO) nanocomposites without using any reductants or surfactants. This approach leads to the reduction of graphene oxide (GO) and the growth of TiO2 nanorods simultaneously. The results reveal that the TiO2 short nanorods with average size of 15 nm in length and 10 nm in diameter were uniformly grown on the rGO sheets with high dispersion. Compared with the pure TiO2 nanoparticles, the TiO2/rGO composites exhibit strong absorption in the visible light range. The photocurrent density of nanocomposites reached 0.826 mA/cm2 under the simulated sunlight illumination, which is 3.5 times than that of pure TiO2. An enhancement of photocatalytic hydrogen formation rate for the water splitting was observed over the TiO2/rGO composite photocatalysts, the fastest formation rate can reach 889.28 µmol·g-1·h-1 when TiO2 coupling with 1wt.% rGO. Also the hydrogen production rate is about 3.27 times larger than pure TiO2 and 2.23 times than P25 due to the excellent electron trapping and transportation properties of rGO and the synergistic effect between TiO2 and rGO.
Wang Jing,Wang Yu,Kong Chang,Liang Yan,Song Wankun,Li Yuhua 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.4
The NAC transcription factor family plays a crucial role in stress response, plant development, and anthocyanin pigmentation. In turnip, anthocyanin biosynthesis is induced by UV-A or blue + UV-B light. However, characterization of turnip NACs is lacking, and whether they are involved in light-dependent anthocyanin accumulation is still unknown. In this study, we identified 200 BrNAC transcription factors in the turnip genome. They were intensively distributed on chromosomes A03, A07, A09, and A10. Phylogenetic analysis classified the turnip BrNAC gene family into 11 subfamilies. The protein subdomains were highly conserved between turnip and Arabidopsis thaliana, and exhibited a close evolutionary relationship. Protein-protein interaction prediction and RNA-seq expression profiles illustrated that the UV-A light responding genes BrNAC183, BrNAC195, and the blue + UV-B light responding gene BrNAC184 were the candidate genes associated with light-dependent anthocyanin accumulation in turnip. We discussed the findings on the relationship between the structure and function of the short-wavelength light responsive BrNAC family proteins from our results and the published data. Our results will aid further functional analysis of BrNAC family transcription factors. This research supports that BrNAC transcription factors regulate light-dependent anthocyanin biosynthesis in turnip.
Xiaonan Fang,Lin-Bai Ye,Yijuan Zhang,Baozong Li,Shanshan Li,Lingbao Kong,Yuhua Wang,Hong Zheng,Wei Wang,Zhenghui Wu 한국미생물학회 2006 The journal of microbiology Vol.44 No.5
GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N)interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted.
Fang, Xiaonan,Ye, Lin-Bai,Zhang, Yijuan,Li, Baozong,Li, Shanshan,Kong, Lingbao,Wang, Yuhua,Zheng, Hong,Wang, Wei,Wu, Zhenghui The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.5
GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N) interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted.
Shuang Li,Bo Wang,Shun Jiang,Xiaohui Lan,Yongbo Qiao,Jiaojiao Nie,Yuhe Yin,Yuhua Shi,Wei Kong,Yaming Shan 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.1
Background: Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide. Objectives: A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy. Methods: In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) in vitro and in vivo after gene transfer. Results: The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2. Conclusions: The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression.