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( Li Xin Deng ),( Yue Mao Shen ),( Si Yang Song ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.1
The pabS gene of Agaricus bisporus 02 encoding a putative PABA synthase was cloned, and then the recombinant protein was expressed in Escherichia coli BL21 under the control of the T7 promoter. The enzyme with an N-terminal GST tag or His tag, designated GST-AbADCS or His-AbADCS, was purified with glutathione Sepharose 4B or Ni Sepharose 6 Fast Flow. The enzyme was an aminodeoxychorismate synthase, and it was necessary to add with an aminodeoxychorismate lyase for synthesizing PABA. AbADCS has maximum activity at a temperature of approximately 25oC and pH 8.0. Magnesium or manganese ions were necessary for the enzymatic activity. The Michaelis-Menten constant for chorismate was 0.12 mM, and 2.55 mM for glutamine. H2O2 did distinct damage on the activity of the enzyme, which could be slightly recovered by Hsp20. Sulfydryl reagents could remarkably promote its activity, suggesting that cysteine residues are essential for catalytic function.
Nematicidal Activity and Chemical Component of Poria cocos
Li, Guo-Hong,Shen, Yue-Mao,Zhang, Ke-Qin The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.1
Poria cocos, a famous traditional Chinese medicine, was found to have nematicidal activity in experiments searching for nematicidal fungi. The experiment showed it could kill 94.9% of the saprophytic nematode, Panagrellus redivivue, 92.6% of the root-knot nematode, Meloidogyne arenaria, and 93.5% of the pine nematode, Bursaphelenchus xylophilus, on PDA plate within 12 hours. According to the nematicidal activity, three new compounds, 2, 4, 6-triacetylenic octane diacid, 2, 4, 5, 6-tetrahydroxyhexanoic acid and 3, 4-dihydroxy-2-keto-n-butyl 2,4,5,6-tetrahydroxyhexanate, were isolated from submerged cultures of Poria cocos. Of these, 2, 4, 6-triacetylenic octane diacid could kill 83.9% Meloidogyne arenaria and 73.4% Panagrellus redivivus at 500 ppm within 12 hours. Here, it is reported for the first time that Poria cocos has nematicidal activity.
New Amide N-glycosides of Ansamitocins Identified from Actinosynnema pretiosum
Juan Ma,Pei-Ji Zhao,Yue-Mao Shen 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.6
By using preparative TLC as the critical isolation procedure, two compounds, including one new amide N-glycosides of ansamitocin (2), were isolated from Actinosynnema pretiosum. The compounds were elucidated as N-demethyl-N-β-D-glucopyranosyl ansamitocin P-2, named ansamitocinoside P-2 (1), and N-demethyl-N-β-D-glucopyranosyl ansamitocin P-1, named ansamitocinoside P-1 (2) on the basis of their spectral data. The 1H-NMR and 13C-NMR assignments were made for 1 and 2 while the 13C-NMR assignment for 1 was revised. Bioassay results showed that 1 had antineoplastic activity.
New Amide N-glycosides of Ansamitocins Identified from Actinosynnema pretiosum
Ma, Juan,Zhao, Pei-Ji,Shen, Yue-Mao 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.6
By using preparative TLC as the critical isolation procedure, two compounds, including one new amide N-glycosides of ansamitocin (2), were isolated from Actinosynnema pretiosum. The compounds were elucidated as N-demethyl-N-${\beta}$-D-glucopyranosyl ansamitocin P-2, named ansamitocinoside P-2 (1), and N-demethyl-N-${\beta}$-D-glucopyranosyl ansamitocin P-1, named ansamitocinoside P-1 (2) on the basis of their spectral data. The $^1$H-NMR and $^{13}$C-NMR assignments were made for 1 and 2 while the $^{13}$C-NMR assignment for 1 was revised. Bioassay results showed that 1 had antineoplastic activity.
Aporphine Alkaloids from Clematis parviloba and Their Antifungal Activity
Jia-Hui Chen,Zhi-Zhi Du,Yue-Mao Shen,Yong-Ping Yang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.1
A new aporphine alkaloid, β-magnoflorine (1), together with a known aporphine alkaloid, α-magnoflorine (2), were isolated from the aerial parts of Clematis parviloba. Their structures were elucidated on the basis of comprehensive spectroscopic techniques. In addition, both compounds showed potent antifungal activities against Penicillium avellaneum UC-4376.
Lin Zhao,Peng-fei Li,Chun-hua Lu,Shu-guang Li,Yue-mao Shen,Yue-zhong Li 한국미생물학회 2010 The journal of microbiology Vol.48 No.4
3-O-α-D-ribofuranosyl epothilone A (epothiloneoside A) is a major component of glycosylated epothilones in Sorangium cellulosum strain So0157-2. The production and glycosylation ratios of epothiloneoside A in both solid and liquid culture conditions with various pH values and carbon sources were studied. The results showed that glycosylation occurs whenever epothilones are produced, regardless of changes in pH values,production time curves, and different carbon sources. We suggest that glycosylation is a stable process,paralleling the biosynthesis of epothilones in the So0157-2 strain.
Antibacterial and Antitumor Macrolides from Streptomyces sp. Ls9131
Pei-Ji Zhao,Li-Ming Fan,Guo-Hong Li,Na Zhu,Yue-Mao Shen 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.11
Four compounds, including two novel macrolides, were isolated from an endophyte Streptomyces sp. ls9131 of Maytenus hookeri. Spectral data indicated that these compounds were dimeric dinactin (1), dimeric nonactin (2), cyclo-homononactic acid (3), and cyclo-nonactic acid (4). Bioassay results showed that dimeric dinactin had strong antineoplastic activity and antibacterial activity.
Antibacterial and Antitumor Macrolides from Streptomyces sp. Is9131
Zhao Pei-Ji,Fan Li-Ming,Li Guo-Hong,Zhu Na,Shen Yue-Mao The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.11
Four compounds, including two novel macrolides, were isolated from an endophyte Streptomyces sp. Is9131 of Maytenus hookeri. Spectral data indicated that these compounds were dimeric dinactin (1), dimeric nonactin (2), cyclo-homononactic acid (3), and cyclo-nonactic acid (4). Bioassay results showed that dimeric dinactin had strong antineoplastic activity and antibacterial activity.
Rational Biosynthetic Engineering for Optimization of Geldanamycin Analogues
Kim, Woncheol,Lee, Dongho,Hong, Seong Su,Na, Zhu,Shin, Jin Chul,Roh, Su Heun,Wu, Cheng-Zhu,Choi, Oksik,Lee, Kyeong,Shen, Yue-Mao,Paik, Sang-Gi,Lee, Jung Joon,Hong, Young-Soo WILEY-VCH Verlag 2009 Chembiochem Vol.10 No.7
<P>Tailor made: We report the rational biosynthesis of C15 hydroxylated non-quinone geldanamycin analogues by site-directed mutagenesis of the geldanamycin polyketide synthase (PKS), together with a combination of post-PKS tailoring genes. Rational biosynthetic engineering allowed the generation of geldanamycin derivatives, such as DHQ3 illustrated in the figure, which had superior pharmacological properties in comparison to the parent compound. <img src='wiley_img/14394227-2009-10-7-CBIC200800763-content.gif' alt='wiley_img/14394227-2009-10-7-CBIC200800763-content'> </P><P>A rational biosynthetic engineering approach was applied to the optimization of the pharmacological properties of the benzoquinone ansamycin, geldanamycin. Geldanamycin and its natural or semisynthetic derivatives have the potential to serve as anticancer chemotherapeutic agents. However, these first-generation Hsp90 inhibitors share an unfavorable structural feature that causes both reduced efficacy and toxicity during clinical evaluation. We report the rationally designed biosynthesis of C15 hydroxylated non-quinone geldanamycin analogues by site-directed mutagenesis of the geldanamycin polyketide synthase (PKS), together with a combination of post-PKS tailoring genes. A 15-hydroxyl-17-demethoxy non-quinone analogue, DHQ3, exhibited stronger inhibition of Hsp90 ATPase activity (4.6-fold) than geldanamycin. Taken together, the results of the present study indicate that rational biosynthetic engineering allows the generation of derivatives of geldanamycin with superior pharmacological properties.</P> <B>Graphic Abstract</B> <P>Tailor made: We report the rational biosynthesis of C15 hydroxylated non-quinone geldanamycin analogues by site-directed mutagenesis of the geldanamycin polyketide synthase (PKS), together with a combination of post-PKS tailoring genes. Rational biosynthetic engineering allowed the generation of geldanamycin derivatives, such as DHQ3 illustrated in the figure, which had superior pharmacological properties in comparison to the parent compound. <img src='wiley_img/14394227-2009-10-7-CBIC200800763-content.gif' alt='wiley_img/14394227-2009-10-7-CBIC200800763-content'> </P>