The Magas1 Gene is Involved in Pathogenesis by Affecting Penetration in Metarhizium acridum

( Yue Qing Cao ),( Xiang Xian Zhu ),( Run Jiao ),( Yu Xian Xia ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.7

Appressorium is a specialized infection structure of filamentous pathogenic fungi and plays an important role in establishing a pathogenic relationship with the host. The Egh16/Egh16H family members are involved in appressorium formation and pathogenesis in pathogenic filamentous fungi. In this study, a homolog of Egh16H, Magas1, was identified from an entomopathogenic fungus, Metarhizium acridum. The Magas1 protein shared a number of conserved motifs with other Egh16/Egh16H family members and specifically expressed during the appressorium development period. Magas1-EGFP fusion expression showed that Magas1 protein was not localized inside the cell. Deletion of the Magas1 gene had no impact on vegetative growth, conidiation and appressorium formation, but resulted in a decreased mortality of host insect when topically inoculated. However, the mortality was not significant between the Magas1 deletion mutant and wild-type treatment when the cuticle was bypassed by injecting conidia directly into the hemocoel. Our results suggested that Magas1 may influence virulence by affecting the penetration of the insects` cuticle.

서울시 생활인구의 시간대별 혼합수준에 영향을 미치는 요인 분석

조월(Cao, Yue),하재현(Ha, Jaehyun),이수기(Lee, Sugie) 대한국토·도시계획학회 2021 國土計劃 Vol.56 No.1

서울시 POI 빅데이터를 활용한 도시활력과 영향요인 분석

조월(Cao, Yue),이수기(Lee, Sugie) 대한국토·도시계획학회 2021 國土計劃 Vol.56 No.7

Characterization and quantification of electron donating capacity and its structure dependence in biochar derived from three waste biomasses

Zhang, Yue,Xu, Xiaoyun,Cao, Lingzhi,Ok, Yong Sik,Cao, Xinde Elsevier 2018 CHEMOSPHERE - Vol.211 No.-

<P><B>Abstract</B></P> <P>Biochar has shown a unique electrochemical property being involved in various redox reactions in soil and water. In this study, the electron donating capacities (EDCs) of biochar pyrolyzed at 200–800 °C from pine wood, barley grass and wheat straw were investigated by using the mediated electrochemical oxidation method. The EDC values for all biochar were in the range of 0.18–1.83 mmol e<SUP>−</SUP> (g biochar)<SUP>−1</SUP>, showing the increase as the temperature increased from 200 °C to 400 °C, the decrease from 400 °C to 650 °C, and then increase from 650 °C until to 800 °C. At low and intermediate temperatures of 200–650 °C, the EDCs were mainly attributed to the phenolic hydroxyl groups, while the conjugated π-electron system associated with aromatic structure dominated the EDCs of biochar at the high temperatures of over 650 °C. The barley grass- and wheat straw-derived biochar had higher EDCs than the pine wood-derived biochar, resulting from the higher phenolic hydroxyl groups in the former samples than the latter one. In conclusion, the reductive property of biochar was mainly attributed to both phenolic hydroxy group and conjugated π-electron system associated with aromatic structure, depending on the pyrolytic temperature and feedstock source. The results will help us to obtain a complete view on the role of biochar in biogeochemical redox reactions and consider developing biochar with controlled redox properties for specific environmental applications such as electron shuttle and catalyst material.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Quantification of the relationship between EDCs and phenolic groups and conjugate π-electron system were reported. </LI> <LI> At low and intermediate temperatures, the EDCs were mainly attributed to the phenolic groups. </LI> <LI> At high temperatures, the conjugated π-electron system associated with aromatic structure dominated the EDCs of biochar. </LI> <LI> Developing biochar with controlled redox properties was proposed for specific environmental applications. </LI> </UL> </P>

Organ-sparing Surgery in Treating Patients with Liposarcoma of the Spermatic Cord: Institutional Experience and Pooled Analysis

Cao, Ming-Xin,Chen, Jie,Zhang, Jun-Long,Wei, Xin,Liang, Yue-You Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Background: Liposarcoma of the spermatic cord is rare and frequently misdiagnosed. The standard therapeutic approach has been radical inguinal orchiectomy with wide local resection of surrounding soft tissues. The current trend of organ preservation in the treatment of several cancers has started to evolve. Herein we present our testis-sparing surgery experience in the treatment of spermatic cord liposarcoma and a pooled analysis on this topic. Materials and Methods: Clinical information from patient receiving organ-sparing surgery was described. Clinical studies evaluating this issue were identified by using a predefined search strategy, e.g., Pubmed database with no restriction on date of published papers. The literature search used the following terms: epidemiology, surgery, chemotherapy, radiotherapy, testis sparing surgery, spermatic cord sarcomas/liposarcomas. Results: Patient received a complete excision of the lesion, preserving the spermatic cord and the testis. The final pathological report showed a well differentiated liposarcoma with negative surgical margins and no signs of local invasion. After 2-year of follow-up, there was no evidence of local recurrence. Since the first case reported in 1952, a total of about 200 well-documented spermatic cord liposarcoma cases have been published in English literature. Among these patients, only three instances were reported to have received an organ-sparing surgery in the treatment of spermatic cord liposarcoma. Conclusions: Radical inguinal orchiectomy and resection of the tumor with a negative microscopic margin is the recommended treatment for liposarcoma of the spermatic cord. But for small, especially well-differentiated, lesions, testis-sparing surgery might be a good option if an adequate negative surgical margin is assured.

The study of the life cycle of small and medium-sized enterprises based on external environmental perspective—empirical research which takes 5 metropolises including Shenzhen as samples

CAO Yu,Wang Fu-Qiang,Ma Yue-ru 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

Based on the research of small and medium-sized enterprises’ life cycle, plus the data and information that acquired from cancelled small and medium ?sized enterprises in five metropolises including Shenzhen, Guangzhou, Changesh, Zhengzhou and Chengdu during period 200-2007, the relationship between the life cycle of small and medium-sized enterprises in the five metropolises and their external environment is evaluated and comparatively analyzed. The results shows that the external environment has positive impact on SMEs’ lifecycle, and economic environment, technological environment as well as human resources environment have prominent influences on SEMs’ lifecycle, but the influences of political environment, socioculture environment and natural resources environment on SEMs’ lifecycle are inconspicuous Further more, the results above is analyzed and the countermeasures on how to prolong the llife cycle of small and medium-sized enterprise in our country from the perspectives of both government and corporation are proposed

Expression profiles of circular RNAs in sheep skeletal muscle

Cao, Yang,You, Shuang,Yao, Yang,Liu, Zhi-Jin,Hazi, Wureli,Li, Cun-Yuan,Zhang, Xiang-Yu,Hou, Xiao-Xu,Wei, Jun-Chang,Li, Xiao-Yue,Wang, Da-Wei,Chen, Chuang-Fu,Zhang, Yun-Feng,Ni, Wei,Hu, Sheng-Wei Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.10

Objective: Circular RNAs (circRNAs) are a newfound class of non-coding RNA in animals and plants. Recent studies have revealed that circRNAs play important roles in cell proliferation, differentiation, autophagy and apoptosis during development. However, there are few reports about muscle development-related circRNAs in livestock. Methods: RNA sequencing analysis was employed to identify and annotate circRNAs from longissimus dorsi of sheep. Reverse transcription followed by real-time quantitative (q) polymerase chain reaction (PCR) analysis verified the presence of these circRNAs. Targetscan7.0 and miRanda were used to analyse the interaction of circRNA-microRNA (miRNA). To investigate the function of circRNAs, an experiment was conducted to perform enrichment analysis hosting genes of circRNAs using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathways. Results: About 75.5 million sequences were obtained from RNA libraries of sheep skeletal muscle. These sequences were mapped to 729 genes in the sheep reference genome. We identified 886 circRNAs, including numerous circular intronic RNAs and exonic circRNAs. Reverse transcription PCR (RT-PCR) and DNA sequencing analysis confirmed the presence of several circRNAs. Real-Time RT-PCR analysis exhibited resistance of sheep circRNAs to RNase R digestion. We found that many circRNAs interacted with muscle-specific miRNAs involved in growth and development of muscle, especially circ776. The GO and KEGG enrichment analysis showed that hosting genes of circRNAs was involved in muscle cell development and signaling pathway. Conclusion: The study provides comprehensive expression profiles of circRNAs in sheep skeletal muscle. Our study offers a large number of circRNAs to facilitate a better understanding of their roles in muscle growth. Meanwhile, we suggested that circ776 could be analyzed in future study.

Simultaneous Determination of Hydroquinone and Catechol by N-doped Porous Biochar-modified Electrode

Yue-Xin Liu,Shi-Man Du,Jie Cao,Wen-sheng Huang,Xiao-Ru Zhang,Bao-Ping Qi,Sheng-Hui Zhang 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.3

N-doped porous biochar (NPB) with large conjugated systems could not only be used as enrichment carriers but also be in favor of electron transport in the electrochemical detection. The NPB-modified electrode was fabricated for the simultaneous detection of catechol (CA) and hydroquinone (HQ) to enhance the signal-to-noise ratio and further improve the sensitivity. A detection limit as low as 37 and 47?nM was achieved for CA and HQ, respectively. The proposed strategy with the merits of high sensitivity, selectivity, and reproducibility exhibited a great potential for the detection.

A New Static Data Flow Clustering Algorithm for Task Scheduling of Irregular Mesh in NoCs Based on Complex Networks

Yue Liu,MengMeng Cao,Kong Jie 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.9

The majority of recent embedded systems are based on MPSoCs (Multi-Processors System on Chip) architectures. The topologies and the interconnections inside multi processors almost adopt NoCs (Networks on Chip) whose topology and task scheduling algorithm have a direct impact on its performances. In this paper, by using static data flow, a task scheduling algorithm which would automatically assign the application tasks onto different processors is proposed based on complex network. The goal of our algorithm is to replace the static data flow subnetwork by a single dynamic data flow actor such that the global performance in terms of latency and throughput is optimized. Through complex network, it greatly enhances the power of our algorithm in terms of avoiding deadlock, saving energy and providing for integration with more general models of computation. Experimental results show up to 60% performance improvement for real-world examples.

Preparation and characteristics of carboplatin-Fe@C-loaded chitosan nanoparticles with dual physical drug-loaded mechanisms

Yue-Hua Guo,Fu-Rong Li,Shi-Yun Bao,Tao Han,Jun-Jian Cao,Han-Xin Zhou 한국물리학회 2007 Current Applied Physics Vol.7 No.s1

The present work is intended to set up the optimal carboplatin-Fe@C-loaded chitosan nanoparticles method and to compare andassess carboplatin-Fe@C-loaded with carboplatin-Fe-loaded chitosan nanoparticles. Both kinds of nanoparticles were prepared by areverse microemulsion method. The carboplatin-Fe@C-loaded chitosan nanoparticles consisted of Fe@C nanopowder with the adsorbeddrug as the magnetic core, chitosan as the matrix and carboplatin as the model drug. The core of the carboplatin-Fe-loaded chitosannanoparticles was pure iron nanopowder, which was unable to adsorb a drug. The characteristics of both kinds of nanoparticles weredetermined and compared. The results showed that both kinds of nanoparticles were spherical in shape with an average size of210 nm ± 26 nm (size range 150300 nm) and a good magnetic responsivity. The drug content of the nanoparticles wasrespectively. The cumulative release percentages of carboplatin-Fe@C-loaded chitosan nanoparticles in vitro in 1d, 2d, 3d, 4d were60%, 74%, 84%, and 92%, respectively, and those of carboplatin-Fe-loaded chitosan nanoparticles in 1d, 2d were 81% and 91%. Thus,the carboplatin-Fe@C-loaded chitosan nanoparticles with dual physical drug-loaded mechanisms (physical encapsulation and adsorp-tion of active carbon) possessed a higher drug content and showed more sustained releasing. The cooperation of multiple mechanismswas a promising feature to improve the properties of nanoparticles.