Metabolism of α-Actin, Expression of α-Actin mRNA and MHC mRNA Induced by Exhaustive Eccentric Exercise in Skeletal Muscles of Rats and Effects of Acupuncture on Them

Wang, Rui Yuan,Yuan, Yu He,Zhang, Wen Dong,Zhao, Lin,Xun, Chuu Yan 한국체육무용국제교류학회 2001 한국체육무용국제교류학회지 Vol.7 No.-

The purpose of this study is to investigate the changes of α-actin metabolism, α-actin mRNA and MHC mRNA expression in skeletal muscles of rats after one set exhaustive eccentric exercise and the effects of acupuncture on them. 130 adult male SD rats were randomly divided into the sedentary group and the exercise group. The rats of the exercise group were again divided into the acupuncture group and non-acupuncture group. According to the sampling time, the exercise group (the acupuncture and non-acupuncture group) was divided into 12 groups, that is the 0 hours, 6 hours, 12 hours, 24 hours, 48 hours and 72 hours groups. In each group there were ten rats. The rats weighting 205±23.3 (mean±SE) were maintained at 23±1℃. They were fed in different chests taking food and drink freely. The rats of the exercise group carried out continuous downhill running with the speed of 16m/min for about 4 hours until exhaustion on the animal treadmill (degree -16°). The rats of the acupuncture group were treated with acupuncture immediately after exhaustive eccentric exercise, but those of the sedentary group and the non-acupuncture group were treated without acupuncture. They were the control groups. The muscle samples were taken immediately, 6 hours, 12 hours, 24 hours, 48 hours and 72 hours after exercise. The contents of G-actin, F-actin and T-actin in skeletal muscles of rats were measured by means of Dnase I assay. The gene expression of α-actin m RNA and MHC isoforms mRNA were determined by using the quantitative reverse transcription PCR technology. Compared with the sedentary group, there was no remarkable change of T-actin after exercise. The decrease G-actin could be detected only immediately after exercise. The content of F-actin was significantly higher than that of the sedentary group 0~6 hours after exercise, but 12~24 hours after exercise it decreased to a lower level than that of the sedentary group. A delayed increase of F-actin depolymerization presented after exercise. The F-actin value of the acupuncture group was improved in a lower range than that of the non-acupuncture group 0~6 hours after exercise, but 12~24 hours after exercise it was remarkably higher than those of the non-acupuncture group. It was shown that acupuncture could regulate the F-actin concentration in skeletal muscles of rats after exercise. Furthermore, the regulation might be bilateral. Acupuncture could stop the increase of F-actin delayed depolymerization induced by high loading exercise. The gene expression of α-actin mRNA in skeletal muscles of rats decreased significantly after one set exhaustive exercise. The duration of its recovery was longer than 72 hours. It might be that acupuncture accelerated the recovery process of α-actin mRNA expression after exercise. The MHC mRNA expression of the non-acupuncture group was lower than that of the sedentary group 6~12 hours after exercise. There appeared a delayed excess expression of MHC mRNA 48 hours after exercise. The curve of the change of the total MHC mRNA expression in the acupuncture groups was similar to that in the non-acupuncture group. The duration of recovery of the acupuncture group was shorter, the delayed excess expression of MHC mRNA was ahead of time and its range was higher than that of non-acupuncture group. It was shown that acupuncture could promote the recovery of MHC mRNA expression after exercise of high load

Long Noncoding RNA FBXL19-AS1-Mediated Ulcerative Colitis-Associated Intestinal Epithelial Barrier Defect

Zhao Xun,Cui De-Jun,Yang Liu-chan,Yuan Wen-Qiang,Yan Fang 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.5

BACKGROUND: This study commenced to uncover the role of long non-coding RNA FBXL19 antisense RNA 1 (FBXL19-AS1) in the development of ulcerative colitis (UC) and its possible mechanism. METHODS: FBXL19-AS1 expression in the colonic sigmoid mucosa of UC patients was detected. A colitis model was induced in mice using 5% dextran sodium sulfate. Hematoxylin–eosin staining was performed for histopathological examination. Apoptosis was detected by Tunel staining and tissue fibrosis was detected by immunohistochemistry. Also, intestinal permeability was examined. The concentrations of inflammatory factors IL-1b and IL-18 were detected by enzyme-linked immunosorbent assay. The relationship between FBXL19-AS1, miR-339-3p and RHOB was verified by RNA immunoprecipitation assay and dual luciferase reporter assay. RESULTS: The expression of FBXL19-AS1 was increased in dextran sodium sulfate (DSS)-induced colitis mouse model. FBXL19-AS1 interference or miR-339-3p overexpression inhibited DSS-induced colonic epithelial cell apoptosis and inflammatory response, and improved intestinal epithelial barrier defects, thereby ameliorating DSS-induced colitis injury in mice. FBXL19-AS1 sponged miR-339-3p while miR-339-3p targeted RHOB. Overexpression of RHOB reversed the protective effect of inhibition of FBXL19-AS1 on DSS-induced colitis in mice. CONCLUSION: FBXL19-AS1 reduces miR-339-3p-mediated targeting of RHOB and aggravates intestinal epithelial barrier defect in DSS-induced colitis in mice.

Effects of antifreeze glycoprotein 8 (AFGP8) supplementation during vitrification on the in vitro developmental capacity of expanded bovine blastocysts

Liang, Shuang,Yuan, Bao,Jin, Yong-Xun,Zhang, Jia-Bao,Bang, Jeong Kyu,Kim, Nam-Hyung CSIRO Publishing 2017 Reproduction, fertility, and development Vol.29 No.11

<P> Cryopreservation is an effective method for the long-term storage of valuable germplasm in the field of reproductive research. The present study examined the developmental capacity of post-thaw bovine blastocysts during vitrification after supplementation with antifreeze glycoprotein 8 (AFGP8). Survival and re-expansion rates in culture during the 12 h after thawing were significantly higher in the AFGP8-treated than untreated group. In addition, blastocysts from the AFGP8-treated group exhibited lower rates of apoptosis. Real-time reverse transcription-polymerase chain reaction analysis showed that the expression of the Bcl-2 gene, coding for an anti-apoptotic protein, was increased significantly, whereas the expression of the pro-apoptotic gene Bax was decreased significantly in the AFGP8-treated group. The cellular proliferation rate and mitochondrial membrane potential were significantly higher in post-thaw re-expanded blastocysts from the AFGP8-treated compared with untreated group. In addition, outgrowth potential in post-thaw blastocysts in re-expanded blastocysts after vitrification was significantly increased in the AFGP8-treated compared with untreated group. Together, these results are the first to demonstrate that the addition of AFGP8 during vitrification can help protect bovine blastocysts against chill-induced injury. </P>

NMAAP1 Expressed in BCG-Activated Macrophage Promotes M1 Macrophage Polarization

Qihui Liu,Xun Zhu,Yuan Tian,Xiangfeng Zhao,Haifeng Jing,Qi Xie,Peng Li,Dong Li,Dongmei Yan 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.10

Macrophages are divided into two subpopulations: classically activated macrophages (M1) and alternatively activated macrophages (M2). BCG (Bacilli Calmette-Guérin) activates disabled naïve macrophages to M1 macrophages, which act as inflammatory, microbicidal and tumoricidal cells through cell-cell contact and/or the release of soluble factors. Various transcription factors and signaling pathways are involved in the regulation of macrophage activation and polarization. We discovered that BCG-activated macrophages (BAM) expressed a new molecule, and we named it Novel Macrophage Activated Associated Protein 1 (NMAAP1). 1 The current study found that the overexpression of NMAAP1 in macrophages results in M1 polarization with increased expression levels of M1 genes, such as inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-α), Interleukin 6 (IL-6), Interleukin 12 (IL-12), Monocyte chemoattractant protein-1 (MCP-1) and Interleukin-1 beta (IL-1β), and decreased expression of some M2 genes, such as Kruppel-like factor 4 (KLF4) and suppressor of cytokine signaling 1 (SOCS1), but not other M2 genes, including arginase-1 (Arg-1), Interleukin (IL-10), transforming growth factor beta (TGF-β) and found in inflammatory zone 1 (Fizz1). Moreover, NMAAP1 overexpression in the RAW264.7 cell line increased cytotoxicity against MCA207 tumor cells, which depends on increased inflammatory cytokines rather than cell-cell contact. NMAAP1 also substantially enhanced the phagocytic ability of macrophages, which implies that NMAAP1 promoted macrophage adhesive and clearance activities. Our results indicate that NMAAP1 is an essential molecule that modulates macrophages phenotype and plays an important role in macrophage tumoricidal functions.

Design and Analysis of an Active Balancing Mechanism for a Vertical Axis Washing Machine

Hai-Wei Chen,Xun-Ting Yuan,Zhen Sun,Qiu-Qi Mou,Ming Xiong,Wei-Hua Wang 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.23 No.7

In recent years, active balancing of washing machines has drawn much attention from manufactures. This paper proposes a novel active balancing mechanism for vibration suppression of a vertical axis washing machine which employs water for counteracting the unbalanced laundry. At first, a novel structure with two balancing planes sharing only one set of nozzles for injecting water is designed and the balancing capability of the structure is analyzed. Then, a dynamic model of the active balancing system is constructed. Vibration characteristics of the system including changes of the vibration amplitude and phase angle under different influences are studied. After that, a strategy for identifying the imbalance is proposed and an active control method is designed. At last, a washing machine armed with the active mechanism is manufactured. Both a fixed eccentric block and a pile of randomly distributed laundry are used to test the balancing effect. The results show that lateral vibrations of the washer can be greatly reduced by the active mechanism. Due to the simplicity of the structure and the satisfactory balancing effect, the mechanism proposed has a big potential to be employed in future washing machines.

Comparison of the Fatty Acid Composition and Small Molecular Metabolites between Yanjin Blackbone Chicken and Piao Chicken Meat

Rong Jia,Wen Xun,Guozhou Liao,Yuan Yang,Guiying Wang 한국축산식품학회 2023 한국축산식품학회지 Vol.43 No.6

The fatty acid composition and small molecular metabolites in breast and leg meat of Yanjin blackbone chickens (YBC) and Piao chickens (PC) were detected by gas chromatography-mass spectrometry and liquid chromatography-quadrupole static field orbital trap mass spectrometry. Thirty-two fatty acids were detected, and the total fatty acid content of PC was significantly higher than that of YBC (p<0.05). Oleic acid, linoleic acid, palmitic acid, stearic acid, and arachidonic acid were the main fatty acids in the two chicken varieties, and the composition of fatty acids in the two varieties were mainly unsaturated fatty acids, being more than 61.10% of the total fatty acids. Meanwhile, 12 and 16 compounds were screened out from chicken legs and chicken breasts of YBC and PC, respectively, which had important contributions to the differences between groups.

Value Distribution of L-functions and a Question of Chung-Chun Yang

Li, Xiao-Min,Yuan, Qian-Qian,Yi, Hong-Xun Department of Mathematics 2021 Kyungpook mathematical journal Vol.61 No.3

We study the value distribution theory of L-functions and completely resolve a question from Yang [10]. This question is related to L-functions sharing three finite values with meromorphic functions. The main result in this paper extends corresponding results from Li [10].

Spectral Analysis of CO2 Corrosion Product Scales on 13Cr Tubing Steel

( Guan Fa Lin ),( Xun Yuan Xu ),( Zhen Quan Bai ),( Yao Rong Feng ) 한국부식방식학회(구 한국부식학회) 2008 Corrosion Science and Technology Vol.7 No.4

Dynamic performance of girder bridges with explosion-proof and aseismic system

Jingyu Wang,Wancheng Yuan,Xun Wu,Kai Wei 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.61 No.3

Recently, the transportation of dangerous explosive goods is increasing, which makes vehicle blasting accidents a potential threat for the safety of bridge structures. In addition, blasting accidents happen more easily when earthquake occurs. Excessive dynamic response of bridges under extreme loads may cause local member damage, serviceability issues, or even failure of the whole structure. In this paper, a new explosion-proof and aseismic system is proposed including cable support damping bearing and steel-fiber reinforced concrete based on the existing researches. Then, considering one 40m-span simply supported concrete T-bridge as the prototype, through scale model test and numerical simulation, the dynamic response of the bridge under three conditions including only earthquake, only blast load and the combination of the two extreme loads is obtained and the applicability of this explosion-proof and aseismic system is explored. Results of the study show that this explosion-proof and aseismic system has good adaptability to seism and blast load at different level. The reducing vibration isolation efficiency of cable support damping bearing is pretty high. Increasing cables does not affect the good shock-absorption performance of the original bearing. The new system is good at shock absorption and displacement limitation. It works well in reducing the vertical dynamic response of beam body, and could limit the relative displacement between main girder and capping beam in different orientation so as to solve the problem of beam falling. The study also shows that the enhancement of steel fibers in concrete could significantly improve the blast resistance of main beam. Results of this paper can be used in the process of antiknock design, and provide strong theoretical basis for comprehensive protection and support of girder bridges.

NMAAP1 Expressed in BCG-Activated Macrophage Promotes M1 Macrophage Polarization

Liu, Qihui,Tian, Yuan,Zhao, Xiangfeng,Jing, Haifeng,Xie, Qi,Li, Peng,Li, Dong,Yan, Dongmei,Zhu, Xun Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.10

Macrophages are divided into two subpopulations: classically activated macrophages (M1) and alternatively activated macrophages (M2). BCG (Bacilli Calmette-$Gu{\acute{e}}rin$) activates disabled $na{\ddot{i}}ve$ macrophages to M1 macrophages, which act as inflammatory, microbicidal and tumoricidal cells through cell-cell contact and/or the release of soluble factors. Various transcription factors and signaling pathways are involved in the regulation of macrophage activation and polarization. We discovered that BCG-activated macrophages (BAM) expressed a new molecule, and we named it Novel Macrophage Activated Associated Protein 1 (NMAAP1). 1 The current study found that the overexpression of NMAAP1 in macrophages results in M1 polarization with increased expression levels of M1 genes, such as inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-${\alpha}$), Interleukin 6 (IL-6), Interleukin 12 (IL-12), Monocyte chemoattractant protein-1 (MCP-1) and Interleukin-1 beta (IL-$1{\beta}$), and decreased expression of some M2 genes, such as Kruppel-like factor 4 (KLF4) and suppressor of cytokine signaling 1 (SOCS1), but not other M2 genes, including arginase-1 (Arg-1), Interleukin (IL-10), transforming growth factor beta (TGF-${\beta}$) and found in inflammatory zone 1 (Fizz1). Moreover, NMAAP1 overexpression in the RAW264.7 cell line increased cytotoxicity against MCA207 tumor cells, which depends on increased inflammatory cytokines rather than cell-cell contact. NMAAP1 also substantially enhanced the phagocytic ability of macrophages, which implies that NMAAP1 promoted macrophage adhesive and clearance activities. Our results indicate that NMAAP1 is an essential molecule that modulates macrophages phenotype and plays an important role in macrophage tumoricidal functions.